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  • Determination of Lods New

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    LuisaFT
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    This document discusses methods for calculating limits of detection (LOD) and limits of quantification (LOQ) for analytical methods and instruments. The LOD can be calculated using the standard deviation of the calibration curve (Sy) and the slope (S) with the formula LOD = 3.3(Sy/S). The LOQ is calculated similarly using the formula LOQ = 10(Sy/S). These values of Sy and S can be obtained from the LINEST, SLOPE, and STEYX functions in Microsoft Excel by analyzing the calibration curve data.

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    Importante para los limites de detección.

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    This document discusses methods for calculating limits of detection (LOD) and limits of quantification (LOQ) for analytical methods and instruments. The LOD can be calculated using the standard deviation of the calibration curve (Sy) and the slope (S) with the formula LOD = 3.3(Sy/S). The LOQ is calculated similarly using the formula LOQ = 10(Sy/S). These values of Sy and S can be obtained from the LINEST, SLOPE, and STEYX functions in Microsoft Excel by analyzing the calibration curve data.

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    © All Rights Reserved
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    8 views1 page

    Determination of Lods New

    Uploaded by

    LuisaFT
    This document discusses methods for calculating limits of detection (LOD) and limits of quantification (LOQ) for analytical methods and instruments. The LOD can be calculated using the standard deviation of the calibration curve (Sy) and the slope (S) with the formula LOD = 3.3(Sy/S). The LOQ is calculated similarly using the formula LOQ = 10(Sy/S). These values of Sy and S can be obtained from the LINEST, SLOPE, and STEYX functions in Microsoft Excel by analyzing the calibration curve data.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    Determination of LODs (limits of detection) and LOQs (limit of quantification)

    LOD’s may also be calculated based on the standard deviation of the response (Sy) of
    the curve and the slope of the calibration curve (S) at levels approximating the LOD
    according to the formula: LOD = 3.3(Sy/S). The standard deviation of the response can be
    determined based on the standard deviation of y-intercepts of regression lines. Note: the
    slope and S can be obtained with one order of magnitude of calibration curve.

    The calculation method is again based on the standard deviation of the response
    (SD) and the slope of the calibration curve (S) according to the formula: LOQ = 10(Sy/S).
    Again, the standard deviation of the response can be determined based on the standard
    deviation of y-intercepts of regression lines.

    The values of Sy and slope can be obtained form the LINEST function (Shown in the
    next figure), when creating calibration curve in the MS Excel. The Sy of y is the standard
    deviation used for LOD and LOQ calculation. Another way to obtain S and Sy values slope is
    to use in MS Excel “SLOPE” and STEYX functions.
    If you are not familiar with detailed use of the LINEST function, follow the document
    calibration curve.pdf

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