1 2 3 4 5 6 7 8 9

A 0.103 0.357 1.678 1.883 1.575 1.917 2.105 1.814 1.876

B 0.095 0.35 0.526 1.651 1.851 1.948 2.11 1.678 1.79
C 0.132 0.606 1.537 1.718 1.939 1.505 2.019 1.82 1.88
D 0.146 0.627 0.641 1.69 1.632 1.716 1.98 1.553 1.818
E 0.162 1.083 1.483 1.699 1.912 1.958 1.737 0.149 1.654
F 0.159 1.031 1.547 1.695 1.712 1.875 1.711 1.616 1.796
G 0.226 1.669 1.415 1.566 1.844 1.584 1.952 1.607 1.715
H 0.231 1.632 1.388 1.454 1.582 1.761 1.736 1.708 1.824

MEAN
0.103 0.095

0.132 0.146
1.8
0.162 0.159
1.6
f(x) = 0.120217168943631 exp( 0.65403753687975
0.226 0.231
1.4 R² = 0.976841120665918
1.2
0.357 0.35
1
0.606 0.627
0.8
0.6 1.083 1.031
0.4
1.669 1.632
0.2
Conc LOG abs
0
0 0 0.099
-0.5 0 0.5 1 1.5 2 2.5 3 3.5 4
0.78 -0.248461 0.139
1.56 0.444686 0.1605
3.13 1.141033 0.2285
6.25 1.832581 0.3535
12.5 2.525729 0.6165
25 3.218876 1.057
50 3.912023 1.6505 3.912023
FORMULA =(LN(absorbance)-(LN(0.12)))/0.654
Formula= exp(value found out after the above formula) This is Concentration in n
1.678 4.033434 56.45447
0.526 2.259648 9.579712
1.537 3.899229 49.36439
0.641 2.561984 12.96151
1.483 3.844542 46.73728
1.547 3.909145 49.85633
1.415 3.772772 43.50049
1.388 3.743314 42.23774
1.883 4.209679 67.33489
1.651 4.008631 55.07143
1.718 4.069456 58.52513
1.69 4.04433 57.07296
1.699 4.052452 57.53835
1.695 4.048848 57.33135
1.566 3.927811 50.79564
1.454 3.814345 45.34706
1.575 3.936573 51.2427
1.851 4.18347 65.59309
1.939 4.254489 70.42083
1.632 3.990932 54.10531
1.912 4.233048 68.927
1.712 4.064107 58.21289
1.844 4.177677 65.21417
1.582 3.943354 51.59134
1.917 4.237041 69.2028
1.948 4.26157 70.92124
1.505 3.867059 47.80158
1.716 4.067675 58.42098
1.958 4.269399 71.47868
1.875 4.203168 66.89796
1.584 3.945286 51.6911
1.761 4.107256 60.7797
2.105 4.38009 79.84524
2.11 4.383718 80.13541
2.019 4.316309 74.91159
1.98 4.286484 72.71035
1.737 4.086274 59.5177
1.711 4.063213 58.1609
1.952 4.264706 71.14403
1.736 4.085393 59.46531
1.814 4.152596 63.5989
1.678 4.033434 56.45447
1.82 4.157645 63.92083
1.553 3.915064 50.1523
0.149 0.33097 1.392318
1.616 3.975868 53.29634
1.607 3.967328 52.84315
1.708 4.06053 58.00505
1.876 4.203984 66.95252
1.79 4.132231 62.3168
1.88 4.207241 67.17093
1.818 4.155964 63.81346
1.654 4.011407 55.22451
1.796 4.137348 62.63648
1.715 4.066784 58.36894
1.824 4.161002 64.13577
1.846 4.179334 65.32236
1.625 3.98436 53.75087
1.717 4.068566 58.47305
2.023 4.319335 75.13864
1.796 4.137348 62.63648
1.93 4.247375 69.92166
1.825 4.16184 64.18954
1.63 3.989057 54.00396
1.797 4.138199 62.68982
1.943 4.25764 70.64308
1.731 4.080983 59.20363
1.925 4.243409 69.64487
1.813 4.151753 63.5453
1.556 3.918015 50.30051
1.82 4.157645 63.92083
1.843 4.176847 65.16011
2.165 4.423064 83.35129
1.993 4.29649 73.44157
1.775 4.119364 61.52009
1.335 3.683784 39.79671
1.659 4.016022 55.47998
1.168 3.479444 32.44168
0.103 -0.233582 0.791693
1.718 4.069456 58.52513
 10 11 12
1.846 1.797 2.165
1.625 1.943 1.993
1.717 1.731 1.775
2.023 1.925 1.335
1.796 1.813 1.659
1.93 1.556 1.168 1.678
1.825 1.82 0.103 0.526
1.63 1.843 1.718 1.537
0.641
1.483
1.547
1.415
1.388
1.883
1.651
0.099 1.718
0.139 1.69
0.1605 1.699
0.2285 1.695
0.3535 1.566
31 exp( 0.654037536879754 x )
8 0.6165 1.454
1.057 1.575
1.6505 Column D 1.851
Logarithmic 1.939
(Column D) 1.632
Exponential 1.912
(Column D)
1.712
1.844
1.582
1.917
1.948
1.505
2 2.5 3 3.5 4 4.5
1.716
1.958
1.875
1.584
1.761
2.105
2.11
2.019
This is Concentration in ng/ml 1.98
1.737
1.711
1.952
1.736
1.814
1.678
1.82
1.553
0.149
1.616
1.607
1.708
1.876
1.79
1.88
1.818
1.654
1.796
1.715
1.824
1.846
1.625
1.717
2.023
1.796
1.93
1.825
1.63
1.797
1.943
1.731
1.925
1.813
1.556
1.82
1.843
2.165
1.993
1.775
1.335
1.659
1.168
0.103
1.718