Protein production service

Trimeric Protein Production Service

INQUIRY

Protein trimerization is known as the formation of a protein trimer,

a macromolecular structure consisting of three noncovalently

associated identical or non-identical subunits.

Profacgen provides novel trimeric protein production service by

designing and expressing proteins in disulfide bond-linked

homo-trimeric forms. With years of experience, Profacgen has

accomplished to significantly increase the expression level and

optimize the purification scheme of recombinant trimeric fusion

proteins, the scale can be quickly moved from preclinical stage

towards the bedsides of millions of patients. Our protein

trimerization technology has established a broadly applicable

system for the production and characterization of trimeric proteins

and generated new insights into the assembly and maturation of

trimers that will have implications for the design of an effective

vaccine.

Features of protein trimerization:

 Naturally secreted trimer

 Disulfide bond-linked

 Mammalian expression system

 High purity

 Structurally stable

Currently major Tumor Necrosis Factor (TNF) biologic blockers are

dimeric in structure, whereas some of the TNF family themselves

are homotrimeric in nature. From a structural biology point of view,

a homodimeric structure with a two-fold symmetry cannot perfectly

dock to a homotrimeric structure with a three-fold symmetry, thus

limiting the affinity between the two molecules.

Protein trimerization platform allows secreted protein to be made as

covalently linked homotrimers with greatly increased avidity to their

disease-causing targets, such as the TNF family of cytokines

(TNFRII, TRAIL, etc), HIV gp120/gp41, Influenza HA, DR4, DR5,

sCD4, and ApoAI. Constructs for all of these have already been

made and are in development as biologic drugs against

autoimmune diseases, cancer, AIDS, osteoporosis, and heart

disease.

Reference: Haipeng Liu and Danmei Su, etc. Improvement of

Pharmacokinetic Profile of TRAIL via Trimer-Tag Enhances its

Antitumor Activity in vivo. Sci Rep. 2017; 7: 8953. doi:

10.1038/s41598-017-09518-1.