Rhcg protein

Stable Cell Line Construction for Protein Expression

INQUIRY

When large amount of recombinant protein is required, stable expression line

construction is recommended for reproducible and cost-effective protein
production. Construction of a stable cell line usually takes 3 steps: delivery of
the target gene into the host cell, screening of stable expression clones, and
stability test.

Stable Cell Line Construction for Protein Expression

Features:
High yield and reproducibility
High cell line stability
One-stop service
Service fully customizable
At Profacgen, we use lentiviral vector for efficient gene delivery and to achieve
high integration rate. Limited dilution and antibiotics screening are then applied
to select for high-yield, genetically homogenous stable expression monoclones.
When fluorescent protein is fused to the target protein, we can also use flow
cytometry to screen for positive clones. Profacgen also offers the generation of
Tet-ON inducible stable cell lines for inducible expression of your target gene.
Doxycycline can then be used to induce the expression of your target gene.

To ensure integration of your target gene and stable/inducible expression of

the protein, we can provide PCR, qRT-PCR, microscopy, flow cytometry
analysis and stability test according to your specific requests.

Profacgen provides stable cell line construction service both for research use
and for industrial production. We guarantee well-developed stable cell lines
with robust and reproducible production of your target protein.