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Form 10a
Among the different methods available, mainly Leaf explants of W. somnifera showed higher
cell suspension culture, multiple shoot culture morphogenetic potential in the presence of
and root culture are reported to be the major Kinetin and 6-Benzylaminopurine each at 4
μM/l (Aruna et al., 2010). An efficient protocol
in-vitro culture systems to enhance the
was developed for high regenerative capacity
secondary metabolite production under in-vitro and easy mass propagation from leaf explants
condition. Hence the present investigation has of Withania somnifera (Arumughan and
been formulated to standardize in vitro Gopinath 2013).
propagation technique of Withania somnifera
and to estimate withanolide content in multiple 9. Outline of the technical programme
shoots derived under in vitro condition by
9.1 Materials
incorporating different elicitors.
Seeds of Withania somnifera, variety Arka
8. Important publications on which the aswagandha procured from IIHR Bangalore
study is based will be used for the study.