Florensia Kristaveren

102316028 / CE1

TUGAS NANOMATERIAL (Gambar 11)

Steps :

1. In ImageJ use the file menu to open an image

2. On the toolbar of ImageJ select the line tool. Hold down the shift key and draw a
straight line along the length of the scale bar of the image being as precise as
possible.This is going to be the known distance that we use as a standard to set the
measurements.
3. Select analyze, then set scale. For the known distance type in the distance of your
scale bar, and then enter the units (microns or nm). Check global so that this
measurement is applied to all of the images taken with the same magnification.

4. Select a region of interest by choosing the box tool, to the far left of the line drawing
tool, and draw a box around the area of interest. Only include in the box the
particles you want to analyze.
5. Under the Image tab select crop

6. Under the Image tab select adjust then threshold. Adjust the threshold by sliding the
bars so that only the particles you wish to analyze are selected. You may need to slide
the top bar all the way to the left and then adjust the lower bar to do this, then choose
apply.
7. Under the analyze tab select set measurements and check the measurements you
wish to take.

8. Under analyze select analyze particles. Check display results and other settings you
wish to use. You will want to set a minimum particle size here so that noise is not
included in your data. Then take the measurements.
9. The measurements will show up in a chart. You can then cut and paste these
measurements into excel in order to analyze the data and create graphs.
Perhitungan Excel

Area Mean Min Max Circ. AR Round Solidity

1 0.013 255 255 255 0.527 1.319 0.758 0.899
2 0.031 255 255 255 0.755 1.604 0.624 0.965
3 0.143 255 255 255 0.303 3.538 0.283 0.812
4 2.50E-05 255 255 255 1 1 1 1
5 0.019 255 255 255 0.535 1.182 0.846 0.92
6 0.059 255 255 255 0.854 1.279 0.782 0.969
7 0.139 255 255 255 0.176 1.471 0.68 0.574
8 0.064 255 255 255 0.736 1.056 0.947 0.954
9 0.057 255 255 255 0.764 1.09 0.917 0.945
10 2.50E-05 255 255 255 1 1 1 1
11 2.50E-05 255 255 255 1 1 1 1
12 0.012 255 255 255 0.605 2.434 0.411 0.943
13 0.038 255 255 255 0.731 1.053 0.95 0.931
14 2.50E-05 255 255 255 1 1 1 1
15 0.051 255 255 255 0.869 1.035 0.966 0.958
16 7.50E-05 255 255 255 1 1.464 0.683 0.857
17 0.03 255 255 255 0.452 1.017 0.983 0.87
18 0.065 255 255 255 0.407 1.688 0.592 0.838
19 2.50E-05 255 255 255 1 1 1 1
20 5.00E-05 255 255 255 0.785 2.646 0.378 0.667
21 0.029 255 255 255 0.836 1.06 0.943 0.966
22 0.038 255 255 255 0.675 1.684 0.594 0.958
23 0.031 255 255 255 0.762 1.591 0.629 0.965
24 5.00E-05 255 255 255 1 2 0.5 1
25 2.50E-05 255 255 255 1 1 1 1
 r2 r d
0.00414 0.064344 0.128688
0.009873 0.099361 0.198722
0.045541 0.213404 0.426809
7.96E-06 0.002822 0.005643
0.006051 0.077788 0.155576
0.01879 0.137076 0.274152
0.044268 0.210398 0.420797
0.020382 0.142766 0.285532
0.018153 0.134733 0.269465
7.96E-06 0.002822 0.005643
7.96E-06 0.002822 0.005643
0.003822 0.06182 0.123639
0.012102 0.110009 0.220017
7.96E-06 0.002822 0.005643
0.016242 0.127444 0.254889
2.39E-05 0.004887 0.009775
0.009554 0.097745 0.195491
0.020701 0.143877 0.287754
7.96E-06 0.002822 0.005643
1.59E-05 0.00399 0.007981
0.009236 0.096102 0.192205
0.012102 0.110009 0.220017
0.009873 0.099361 0.198722
1.59E-05 0.00399 0.007981
7.96E-06 0.002822 0.005643

pembulatan
jumlah data 25
min 0.005643
max 0.426809
range 0.421165
jumlah kelas 5.613202 6
interval 0.070194 0.08

Manual (Rumus Excel)

Bin array Frekuensi
0.085643326 9
0.165643326 3
0.245643326 6
0.325643326 5
0.405643326 0
0.485643326 2
 Perhitungan menggunakan Histogram pada Data Analysis

Cumulative Cumulative
Bin array Frequency % Bin array Frequency %
0.085643 9 37.50% 0.085643 9 37.50%
0.165643 2 45.83% 0.245643 6 62.50%
0.245643 6 70.83% 0.325643 5 83.33%
0.325643 5 91.67% 0.165643 2 91.67%
0.405643 0 91.67% 0.485643 2 100.00%
0.485643 2 100.00% 0.405643 0 100.00%
More 0 100.00% More 0 100.00%

Histogram
10 120.00%
9
8 100.00%
7
Frequency

80.00%
6
5 60.00%
4
3 40.00%
2 Frequency
20.00%
1 Cumulative %
0 0.00%

Bin array