High Performance Liquid Chromatography, Altus A-10 Perkin Elmer

Schematic representation of an HPLC unit.

(1) Solvent reservoirs,

(2) Solvent degasser,
(3) Gradient valve,
(4) Mixing vessel for delivery of the mobile
(6) Switching valve in "inject position",
phase, (5) High-pressure pump,
(6') Switching valve in "load position",
(7) Sample injection loop,
(8) Pre-column (guard column),
(9) Analytical column,
(10) Detector (i.e. IR, UV),
(11) Data acquisition,
(12) Waste or fraction collector.
Analysis using empower software from waters over view :
Analysis process :
1. Pre analysis (Sample & Solvent Preparations)
 Making solvent
 Filtering sample & solvent
 Degassing sample & solvent
2. Run analysis (Methode setup & run sample)
 Power supply on
 Computer on
 Main instrument on (sampler and solvent module)
 Detector on (wait until lamp flash off)
 Open empower software on computer desktop
 Log in system
 Method setup
 Run sample
3. Post analysis (reprocess, Review & report)
 process data
 review
 adjust integration & identification
 save result
 preview result
 print result
 log out system
 close empower software
 main instrument off
 detector off
 computer off