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  • Lecture 3: Enzyme Kinetics: Arti Dua Department of Chemistry IIT Madras

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    Mrinal Shankar
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    This document discusses key concepts in enzyme kinetics including the Michaelis-Menten mechanism proposed by Leonor Michaelis and Moud Leonora Menten, using the Michaelis-Menten equation and Lineweaver-Burk equation to determine Km and Vmax values from experimental data, and defining the turnover number as the maximum number of substrate conversions per second by a given enzyme concentration.

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    Enzyme Kinetics

    Original Title

    Lecture3(Chemical Kinetics)

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    This document discusses key concepts in enzyme kinetics including the Michaelis-Menten mechanism proposed by Leonor Michaelis and Moud Leonora Menten, using the Michaelis-Menten equation and Lineweaver-Burk equation to determine Km and Vmax values from experimental data, and defining the turnover number as the maximum number of substrate conversions per second by a given enzyme concentration.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    16 views8 pages

    Lecture 3: Enzyme Kinetics: Arti Dua Department of Chemistry IIT Madras

    Uploaded by

    Mrinal Shankar
    This document discusses key concepts in enzyme kinetics including the Michaelis-Menten mechanism proposed by Leonor Michaelis and Moud Leonora Menten, using the Michaelis-Menten equation and Lineweaver-Burk equation to determine Km and Vmax values from experimental data, and defining the turnover number as the maximum number of substrate conversions per second by a given enzyme concentration.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 8

    Lecture 3 : Enzyme

    Kinetics

    Arti Dua

    Department of Chemistry

    IIT Madras

    !
    Michaelis-Menten Mechanism

    Moud Leonora Menten and Leonor Michaelis


    Enzyme Kinetics
    Enzyme Kinetics
    Enzyme Kinetics
    Enzyme Kinetics
    Enzyme Kinetics

    One can determine Km


    and Vmax from the slope
    and intercept using the
    Lineweaver-Burk Equation.
    .
    Enzyme Kinetics

    Turnover number: Maximum number of chemical conversions


    of substrates to products in a second for a given enzyme
    concentration, E0.

    Vmax
    k2 =
    [E]0
    Turnover number

    Note: The Michaelis-Menten equation, The Lineweaver-Burk


    Equation and turnover number are three important concepts
    in enzyme kinetics.

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