Professional Documents
Culture Documents
RATS
________________________
________________________
In Partial Fulfillment
________________________
RESEARCHERS:
Salvador, Shaira P.
Tamot, Nemalyn A.
Torres, Divine D.
MEDICAL COLLEGES OF NORTHERN PHILIPPINES COLLEGE OF MEDICAL TECHNOLOGY
TABLE OF CONTENTS
Title Page 1
Table of Contents 2
Acknowledgment 4
Dedication 5
Abstract 6
Chapter 1:
Introduction 8
Hypotheses 24
Conceptual Framework 26
Definition of Terms 28
Chapter 2
Methodology 30
Chapter 3
Interpretation of Data 39
Chapter 4
Summary of Findings 44
Conclusions 44
Recommendations 45
Bibliography 46
Appendix 49
Documentation 54
MEDICAL COLLEGES OF NORTHERN PHILIPPINES COLLEGE OF MEDICAL TECHNOLOGY
ACKNOWLEDGEMENT
DEDICATION
ABSTRACT
Based from the data obtained, Groups II, III and IV which
minutes.
hypoglycemic effect.
MEDICAL COLLEGES OF NORTHERN PHILIPPINES COLLEGE OF MEDICAL TECHNOLOGY
Chapter 1
THE PROBLEM AND REVIEW OF RELATED LITERATURE
Introduction
agents have side effects in the long run (Samad et al., 2009).
yet the mechanism of most herbals used has not been defined
alternative drugs and strategies and also may guide the way to
Specific objective:
STZ injection.
response time.
Diabetes
hyperglycemic drug.
(www.diabeteswellness.net).
pancreas does not produce enough insulin or when the body cannot
Mutembei J K; 2014)
and less than 10mmol/L after one and a half hour of meals. At
bed times the normal blood sugar level is about 7-10mmol/L. The
normally, people who harvest and eat these plants would not
rhizomes (roots) that are boiled, dried and then ground into a
(Wickenberg, 2015).
MEDICAL COLLEGES OF NORTHERN PHILIPPINES COLLEGE OF MEDICAL TECHNOLOGY
2,4,5,7-tetrathiaoctane-2,2-dioxide, 2,4,5,7-tetrathiaoctane,
Pioglitazone
reduced the heart rate, mean arterial blood pressure, body mass
Streptozotocin
GLUT2, the toxic action is not specific to β cells and can cause
Albino Rats
Research (FBR).
Most of the mice and rats used in medical trials are inbred
Institute.
1. What is the blood glucose level of the male albino rats before
streptozotocin?
treatment?
Hypotheses
groups.
the market.
country.
Conceptual Framework
Ethanolic Extract from Turmeric and Garlic Ethanolic Extract of Turmeric and Garlic
2-3 months old and acclimatize for one week in order to adapt
rat's tail with a sterile lancet, wiping the first drop of blood,
DEFINITION OF TERMS
Active Constituent
activity.
Blood Glucose
bioassay analysis.
minutes.
Experimental Group
extracts.
Extraction
(Chemicool, 2014)
MEDICAL COLLEGES OF NORTHERN PHILIPPINES COLLEGE OF MEDICAL TECHNOLOGY
fasting.
Glucometer
the mice.
Hyperglycemia
Hypoglycemia
Steptozotocin
Chapter 2
METHODOLOGY
Acclimatization
rendered diabetic rats. Twenty (20) 2 months old male rats will
1. Procurement of rats
2. Acclimatization period
(Tulbaghia violacea)
before gathering and after which leaves will be freed from any
4. Plant Authentication
of the variety.
cut rhizomes will be soaked in 95% ethanol, 1000 mL for each 200
MEDICAL COLLEGES OF NORTHERN PHILIPPINES COLLEGE OF MEDICAL TECHNOLOGY
for 5-6 hours to remove the solvent ethanol. The remaining liquid
7. Streptozotocin Preparation
8.Bioassay
sterile lancet, wiping the first drop of blood, and then placing
induction
treatment.
be determined.
pioglitazone:
The solvent from the total extract will be filtered and then
Identification Test:
The individual extract will be subjected to the qualitative
phytochemical screening for the presence of some chemical
constituents. Phytochemical test will be carried out adopting
standards procedure (Trease et.al 1983, Kokate et.al 1997, Hegde
et.al 2010).
Data Analysis
CHAPTER 3
PRESENTATION, ANALYSIS AND INTERPRETATION OF DATA
activity. The results suggest that when rats were given a dose
Paired difference
Mean Std 95% confidence t Df{decrease Sig
error interval of the of freedom t-
Mean difference tailed
lower upper
and the last 5 rats in the garlic dosage group had a mean of
was not, F (4,12)=2.67. Post hoc analyses using the Tukey HSD
145.8).
rats after 120 minutes was analyzed as before showing the effect
test rats were medicated the extract 30 and 120 minutes response
and garlic extract intake than when they are given isotonic
solution alone.
Discussion
that the B cells could have regenerated awhile thus some amount
glucose intake.
MEDICAL COLLEGES OF NORTHERN PHILIPPINES COLLEGE OF MEDICAL TECHNOLOGY
CHAPTER 4
FINDINGS, CONCLUSION AND RECOMMENDATIONS
Summary
rendered diabetic rats. Twenty (20) 2 months old male rats were
drawn and analyzed with RCBD ANOVA. Results shows a prior blood
Conclusion
hence rats in the turmeric and garlic dosage group had the lower
also conclude that hence pioglitazone did not differ in the mean
Recommendation
caution.
MEDICAL COLLEGES OF NORTHERN PHILIPPINES COLLEGE OF MEDICAL TECHNOLOGY
BIBLIOGRAPHY
Nairobi, Kenya.
India.
Pharmacol Sci.
12-60
Department
Pharmacol Sci.
MEDICAL COLLEGES OF NORTHERN PHILIPPINES COLLEGE OF MEDICAL TECHNOLOGY
APPENDIX
Confidence interval:
The mean of Group One minus Group Two equals -166.70
95% confidence interval of this difference: From -233.29 to
-100.11
Total Mean
D
MEDICAL COLLEGES OF NORTHERN PHILIPPINES COLLEGE OF MEDICAL TECHNOLOGY
Block total 816 527 639 1217 1113 4312
Source of SS Df MS F P
variation
Treatments 109653.2 3 36551.0667 4.42 0.025923
(between 5%=3.49
groups)
Blocks 88273.8 4 22068.45 2.67ns
5%=3.26
Error 99165.8 12 8263.8167
Totals 297092.8 19
treatments B1 B2 B3 B4 B5 Treatment
Total Mean
Isotonic 446 142 281 412 417 1698 339.6
A
Pioglitazone 133 125 106 110 173 647 129.4
B
Turmeric 104 109 118 356 128 813 162.6
C
Garlic 140 113 112 294 327 986 197.2
D
Block total 823 489 617 1172 1045 4144
Block mean 206 122 154 293 261 207
Source of SS Df MS F P
variation
(sum of (mean (Fisher’s
(decrease
Square) square) test)
of
freedom)
DOCUMENTATION
ACCLIMATIZATION
CURRICULUM VITAE
NAME:
AGE:
DATE OF BIRTH:
HOME ADDRESS:
CIVIL STATUS:
RELIGION:
CITIZENSHIP:
PARENTS:
SIBLINGS:
SCHOOLS ATTENTED:
ELEMENTARY:
SECONDARY:
TERTIARY:
MEDICAL COLLEGES OF NORTHERN PHILIPPINES COLLEGE OF MEDICAL TECHNOLOGY
NAME:
AGE:
DATE OF BIRTH:
HOME ADDRESS:
CIVIL STATUS:
RELIGION:
CITIZENSHIP:
PARENTS:
SIBLINGS:
SCHOOLS ATTENTED:
ELEMENTARY:
SECONDARY:
TERTIARY: