Ferric chloride

Group 4
 INTRODUCTION

Proteins are essential to the continuous growth of a

person due to its versatile ability.

In addition, proteins aside from consisting numerous

amino acid, it also consists of many different compounds
that is usually the reason of how each amino acids are
unique.
 INTRODUCTION

In fact, one amino acid- tyrosine- contains an aromatic

compound that is directly attach to an OH group. This
aromatic compound that contains an OH group is
known as a phenol and in this chemical test will have an
important part to play.
 HISTORY
➥In 1834, Runge discovered in the oil of coal tar at least six
new compounds among which was phenol. He discovered
that phenol gave a violet blue coloration with ferric chloride.

➥The first systematic attempt at a study of this reaction was

made in 1871 by Hugo Schiff. His observation led him to
believe that the color obtained by treating a phenol with iron
salts were due to the presence of the hydroxyl group in the
compound
 HISTORY
➥In 1876, O. Hesse endeavored to use the results of Schiff in
determining the structure of a substance which he had
prepared, and which he suspected to be a phenol. He
observed that an aqueous solution of phenol gave a violet
color with ferric chloride while an alcoholic solution of phenol
gave no color at all.
 PROCEDURE
place 5.0 mL of milkon a watch glass and heat over steam bath until a film is
produced on the surface of the liquid.

Using clean and dry stirring rod, remove the film and place a small portion into
it into a clean test tube

Add 5.0 drops of FeCl3 solution

-> To determine the presence or absence of phenol, that would turn violet if
positive
 R E S U LT S
Actual
after heating the milk over a steam bath, a film is produced.
R E S U LT S
Actual
 R E S U LT S
Actual
after adding Ferric Chloride (FeCl3) the film in the test tube became
yellow.
R E S U LT S
Actual
E X P E C T E D R E S U LT S
FA C T O R S T H AT M I G H T H AV E
A F F E C T E D T H E R E S U LT S

1. Heat

2. Composition of Milk

3. Contamination
 PRINCIPLE
The Ferric Chloride Test is used to determine the
presence and absence of phenols in a given sample.

Phenols form a violet complex with Fe(III)+, which is

intensely coloured. The colour may vary from blue, green or
even red depending upon the nature of the phenol.