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List of Annexes June 7, 2018

denotes a draft is available

Chapter 3
3.1 Shipping clinical samples
3.2 Preparation, shipment and extraction protocols for dried blood spots
3.3 Processing oral fluid samples
3.4 Composition of viral transport media, antibiotics and reagents

Chapter 4
4.1 Comparison of IgM EIA kits for measles and rubella

4.2 Testing oral fluid for measles- or rubella-specific IgM by EIA

Chapter 5

5.1 Vero/hSLAM cells: maintenance, propagation, and preparation of frozen cell stock
5.2 Methods for the inoculation of clinical samples for measles or rubella isolation
5.3 Confirmation of rubella virus in culture by IFA and ICA
5.4 Application of viral lysates or viral RNA to FTA cards

Chapter 6

6.1 RNA extraction methods for clinical samples and FTA cards
6.2 CDC real-time measles RT-PCR protocol targeting measles N gene (75 nt region)
6.3 6.4 Real-time RT-PCR for measles vaccine strains
6.4 CDC diagnostic rubella RT-PCR protocol targeting rubella E1 gene (185 nt region)
6.5 CDC real-time rubella RT-PCR protocol targeting rubella p150 gene (154 nt region)
6.6 Agarose gel preparation and clean-up of PCR products

Chapter 7
7.1 Protocol for amplification of measles sequencing window (N-450)
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7.2 Protocol for amplification of measles full length N gene

7.3 Protocol for amplification of measles full length H gene
7.4 Instructions for entering data and navigating MeaNS for sequence submission
7.5 Protocol for amplification of rubella sequencing window (E1)
7.6 7.5 Instructions for entering data and navigating RubeNS for sequence submission

Chapter 9
9.1 High throughput-optimized soluble neutralization assay for measurement of rubella neutralizing
antibody titer

Chapter 11
11.1 Examples of laboratory request/investigation forms

Chapter 12
12.1 Trouble shooting guide for IgM EIAs
12.2 Recommendations for the evaluation of EIAs for IgM detection
12.3 Levey–Jennings control charts
12.4 Determining concordance for confirmatory testing using Siemens assay
12.5 Supplemental information for optimal laboratory design