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  • Glomerular Filtration Rate (Inulin Clearance) Renal Blood Flow (PAH Clearance)

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    The document describes a method for measuring glomerular filtration rate (GFR) and renal blood flow (RBF) in mice. GFR is measured by inulin clearance, which is the most sensitive method for assessing renal function. RBF is measured simultaneously by PAH clearance to detect renal vasoconstriction. The method involves intravenous infusion of inulin and PAH via catheter in anesthetized mice, collection of urine and blood samples, and using colorimetric methods to determine inulin concentrations and calculate clearance rates.

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    The document describes a method for measuring glomerular filtration rate (GFR) and renal blood flow (RBF) in mice. GFR is measured by inulin clearance, which is the most sensitive method for assessing renal function. RBF is measured simultaneously by PAH clearance to detect renal vasoconstriction. The method involves intravenous infusion of inulin and PAH via catheter in anesthetized mice, collection of urine and blood samples, and using colorimetric methods to determine inulin concentrations and calculate clearance rates.

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    28 views1 page

    Glomerular Filtration Rate (Inulin Clearance) Renal Blood Flow (PAH Clearance)

    Uploaded by

    bagasputraiyana
    The document describes a method for measuring glomerular filtration rate (GFR) and renal blood flow (RBF) in mice. GFR is measured by inulin clearance, which is the most sensitive method for assessing renal function. RBF is measured simultaneously by PAH clearance to detect renal vasoconstriction. The method involves intravenous infusion of inulin and PAH via catheter in anesthetized mice, collection of urine and blood samples, and using colorimetric methods to determine inulin concentrations and calculate clearance rates.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    Glomerular Filtration Rate (Inulin Clearance)

    Renal Blood Flow (PAH Clearance)

    Scope of application
    The most sensitive method to measure renal function is glomerular filtration
    rate (GFR) measurement by inulin infusion. For detection of renal
    vasoconstriction renal blood flow (RBF) can be measured simultaneously by
    PAH clearance.

    Method
    Measurement of GFR (inulin clearance) and RBF (PAH clearance) will be
    performed in fully anasthetized mice via intravenous infusion of inulin/PAH
    through the tail vene after a catheter is placed into the bladder. After one hour
    equilibration period, urine is collected for 90 min and blood is drawn in the
    beginning and at the end of the infusion. Infusion solutions for the
    measurement of the GFR and RBF are 15% inulin, 3,75% PAH and 1% bovine
    albumin in 0.9% saline at a rate of 5 µl/min per 100 g body weight. Inulin
    concentrations in urine and plasma aliquots are obtained using the standard
    colorimetric methods.

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