Antimicrobial Activity of ​Tradescantia spathacea​ (BANGKA-BANGKAAN) Crude Leaf

Extract on S. aureus and E. coli

Animas, Louise Danielle, Faenag, Bea Charissa, Lilia, Reynalyn
RANHS - DISTRICT VII - AREVALO

ABSTRACT

This is an experimental study that determined the antimicrobial activity of ​Tradescantia

spathacea​ (Bangka-bangkaan) Crude Leaf Extract against ​S. aureus​ and ​E. coli​. The study was
conducted from August 5 to September 14, 2019. The plants were collected at Ramon Avanceña
National High School, Arevalo, Iloilo City. Leaves were extracted using a juicer and the
supernatant taken was prepared in three concentrations (100%, 75% and 50%) by adding distilled
water. The crude extract was assayed on two test microorganisms (​Staphyloccocus aureus​ and
Escherichia coli)​ with Amoxicillin as positive control and distilled as negative control. The
method used was Agar Well Assay in Muller Hilton Agar to compare the zone of inhibition of
the treatments. One Way Analysis of Variance was used to determine the significant differences
among the treatments. Results showed that (1) ​Tradescanthia spathacea​ Leaf Crude Extract with
100% concentration has created a zone of inhibition with ​S. aureus​. (2) The average zone of
inhibition of ​T. spathacea​ Leaf Crude Extract 100% concentration and Amoxicillin was 14.95
mm and 40.67mm, respectively. (3) 75% and 50% concentrations have no significant difference
with the negative control. (4) All concentrations of ​T. spathacea​ Leaf Crude Extract showed no
sign of inhibition on ​E. coli.​ It is concluded that ​T. spathacea​ leaf crude extract in 100%
concentration has shown antimicrobial activity against ​S. aureus​ but not as comparable to the
available commercial brand and that further and different extraction processes may be conducted
to express its full antimicrobial components and feasibility.
Oil Sorption and Retention Capacity of ​Zea mays​ cob and​ Cocos nucifera​ husks as Potential
Biosorbents for Oil Spill Clean-ups
Sustituido, Jehu James
RANHS - DISTRICT VII - AREVALO

ABSTRACT

This is an experimental study that determined the Oil Sorption and Oil Retention Capacity of ​Zea
mays​ cob and ​Cocos nucifera​ husks that would provide more possible choices that could be used
for remediation of oil spills. The study was conducted from August 13 to September 14, 2019.
Cocos nucifera​ husks and ​Zea mays​ cobs were collected from the School Material Recovery
Facility. The plant samples were washed and dried at 70˚C in drying oven for 6 h and placed in
desiccators. Oil sorption test and Retention Capacity was performed as follows based on ASTM
Standard Method of Testing Sorbent Performance of Adsorbents (F726-99). Human hair served
as the positive control. One Way Analysis of Variance was used to determine the significant
differences among the biosorbents. Results showed ​Cocos nucifera​ husks has higher oil sorption
capacity compared to ​Zea mays​ cob. Human hair had higher oil absorption compared to the two
biosorbents. It was also observed that ​Cocos nucifera​ had highest oil retention capacity, followed
by ​Zea mays​ cob, then Human hair. There is no significant difference between ​Cocos nucifera
husks and Human hair with regards to oil sorption. However, ​Zea mays​ cob shown low level of
oil sorption. ​Cocos nucifera​ husks had the highest oil retention capacity and comparable oil
sorption to human hair making it a possible sorbent for oil spill clean-up. Further research is
suggested especially on the Oil-Water Selectivity of the proposed biosorbents.