_ Biohehnotosy.
Alo + teh log > Joum by kaw eaigy (1870)
fe eae dealer production 4 ry
> te fa the Mae Ley Ouse @r
thy Lbmipoillce tp produce produce and
suwies mas pu. tm humo keting
two
4, Old /troditonat Biotin, —~ bygone 1770
4 New) Modo Divlen
Ola Biotehnetogy : .
fn Ba mama ypitensy eh ving Sagnine
Oe uel dp produer Uaaput product aud sravers
em aTony Yaaurageutes
i by beth
ole q
nolo, enedlo. Laie sca Cutie
aL Ap a"Enter the world of Intelligent Learning & Digital Homework"
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onium —> sel —> desbud ws (nid)
gee Dasa | plasmid)
[Recomtannt SWAT «-DNA
hbrasic DNA
4
Tronspoued, sudo
AuUele hore 2th
OOS
eatin Uti LU te eeabtered!
Olgas ‘ maintained uv culture
Cringe. Cone ; at dale Male
en
Yoo moat Landaate -cliseev.
> atin o HUG endonuclease ergy
a discovery 4 beeen, arate, nicest « fiattng.
exile Lomond 31
4 oli] ond [A Boge]
. ae BEA que
powbiutd, tu autinintis ltt
ebedinuby wrth thy plasrall of - > Sabmometta,
; * wil SNA,Pat. beeyh : he an
A gue. 9} Sv 40 visu > Etat
oy A piage vn
Mees
i fet petmalin of A-DIA
‘ rad cng fe whit, veeliy DNA
> champs of OKA sty sultan, Rout 20
> . auth. iva a Jeoratlinane host cou.
> Maiuininene and ting 4 satan hnaucts
Kew abl aie soap scale to obTariv duired
product.
Y- BNA formation aut a Tanager Yehadque
id +
tools _seyuined a
i= BNA
fassougr / DNA
Hoar cur
ae oa
as
“34°
bth Ugumu9 i Gayyme
Cenjyrte fon tyde ef cebu)
4 “yay (for bacterx)K Oe enone larr eapyeee : [Molecuter Acissoxs |
a eo tn i
pect On dpectyte dete
2 i Reaivicon eugyre dave Lt own specif
diz diseoveud ej. > [hinat_tt | %
oo Harmephitous Saagunangee *
loliv on, ECORT eugymen 6. cow
(oy ftw, Mata aud Arver)
ekevie
> 400 Lypus RE. euggrls
4 eemdat flom sso sain
bun disrvoud fom 230 22
4 basteria [Neer]
RE tagyete
2 fom my
> hae enyymer ever Aula thy DNA q “Ua
gum doactouia cr baetenta mods
reabiotion 4 jh DNA by eben
ttn sien ay mewgyyrmceratlany diese Cag
160& es RE engyme :
pe. doe aud “ype ca
Type-T Uype-a] (Type)
> Net 40 apecipio Mv. speucyic Yype &
e At LU Ld
oe atrand a bot Stroud Type =
4 PNA
> Regudneneent Legquiemout, type T
#
Petey
Stet
S. Adel Mettionire
type T
Ry sbriterit Aults + / / , .
4/3/6 bp ~y | . ,Mode
ObLigue tweeer)
wu DNA near D te 4ue DNA ot tue
LEAT, q podutnidone. LU of Pain coumal
Prod end pe/ aoiky euda Puoduets Atuumt enclt,
E cort Ama“ 2
a eceaaa-
- qoaece-
v
= GAATTC —
~ CTTAAG >
r
L
= 44q | Coo
—¢ce [ee
Lsetotion
y enzyme
po bacteria,
# dlpuawens
Aim4Aiims in2) on en
wpe uta fox sepetlecads 4 DNA
q? RNA P CDNA pw a).
Rue Pauseripkore CRNA DNA)
Wy) Sotut enyyme ( motecrbar ges lqe)
eageyme Aree — ow ny 4 DNA Atqrence,
v T% DNA digas
Wy Bite Capes §
> Alkaline phosphate
> Removes Pg op pom Sled gy DNA
> awed to paneer put eladipation — oY Any
puderz DNA
—
oH 0"
3’ s’TD) Vestn DNA.
> Apican pwn alee Aas ray) Maite Wo
fw Host cel,
« vi
Auton q veelou depenols mo Ag ee
ype ef tose cot
A VeeTBL aniiat have 8 ewe, propertebs
Pater gf Osh - a vector Dna must have
atleast onc ou AO that fe On Atphicale
& dabouie the
pwa [ov RLONA
{' Ariiiieietio ogee
wpe eee pm
(ae
A yuclou. tows hove sustain A
164
\Alins inUp8- plasmid
rae | wm aye
dapaaiig! DSc BRP Aged gon
are
Bam HIT
ter -R
Sat rT
ROP - &
wwtios ‘lipo. tin, moe
4 "a pensetantycecat vector)
BNA MLOMeMUtOUs =
tadtvilopnags
dapectty ? upto ‘Yo! kbp
' pyc Ue
puen@
asthe wT LO Aim4Aiims in————~ mena
L Rewer brane exiption
ee RNA
m-RNA - SMA hyphal
Denarwuration,
———— m-rna
— DNA
| DNA prveeee.
me as DNA
¢-DNA
Cara rd eo
ki) Apethnein BNA pote lien,
7 domypleke ontyeeal —ayritlanrie 4
> da tha » te extue, boss ALG RRL q (
muse bre know ( ne
ade aot Kanaben (96 Sup LicaLiion
“4 ious of bua (template)
.
vitae, DNA
dot m, HG ent eS pe
K bn 1968 > os asian * RNA gory yor
ty bp tong
sron-qqunttiandl...80 eb.
AiméAiims in
schfuats)ta ATF ee Aipettnuiaed Tyrosine + RIA
hie E. cole
07 bp .
BD) Hest 4eees
ry deena Typ (diapers om Aeg, 4 experiment)
Prackun trl
Yeaur
Plant
Aim4Aiims in— A-DNA wumalton and
Y) Sestation of 2 NA
ao ge oy Oey me
hata etn
Tom this, some Large MnBeromatcster Ae
Are ak Atke _protetas
fms EY
be Prgtease RNA it Ribonucliase ete.
ah pups DNA 20 be precipitated
ty) DNA at
obmmiwd deatned fase:
> Wis destied | DNA an be,
obtained, by aly ont the @ muthoas < 20,
> Ak the dame Dimn, & dutibt, Velo, BVA
POLYMERASE CHAIN REACTION (feopte's choice Macbin)
168
Aim4Aiims.inPCR: te & an -AWlomaTd rapid
6] tuvilin DNA Amptigteatimn .
incl, of 1983,
u in vitro DNA Lied isn
y & be i baud mm xp
and denoliialm - sunaluralion propery y Dna
one copy of gent / DNA
t¥ bNe rg mnase
- AL seclaieais
date
f acre
4 41P
dare
| ben efpicteusey ot at wae)
Pun 0
Ver Polrmisade
fiom thvumocereus Lito Aakis
Aim4Aiims inper th a @ abep theumat “gels
+> denaturation (44’c)
om pinaatling (s4*c)
= extension C72"c)
sf s
weno
,
ee ammount of DVR pete double ates Cueuy
BNA moteute 1
@ yaw > ("|9 Yorum alin t A-DNA. oud vector DNA
_% Coast gene/ passenger) DNA, Qe Cue
by ame sustrteDion endonuclease engine
b ote wilh have ot
poly UAB. uk are, te ADI
2 OC iy
oth au miked aud Lipa ew Aad
Vester «BNA Quek forts A-BNA
ae &
ppl TurIIrs,
2
DNA
ani deatied
. —ea—""
Torr
Me ge
“Oo OFWY) Sranapt of ADA ty aultalete hore ett +
Ve comp Lueatid,
deveral method,
* dapanas on enrayell o bore x20.
Yay din Lub mace ompetent
to take ADNA
& taavapr of gene] n- dwn by Matlin elt.
Pactetah cell Yhig create
woth Are ’
* pox aw cell
—> wok baeterta aud
Lio syme and. mn ae
“eet ions Thus aptcdenty ¢
[ee
Aim4Aiims.in* gene x- DNA Pransper et faut cots,
‘omnon de by & plasmic / —_
‘NMasturat gunetiolr form Apesectatee sntgaciens
. Cupineey
Ie eauuses eroum gal diwose
ww dteot
f= monocet
ns ackeim,
Ui~ plasnu’d, > yuner dacdesing plaseta.
Te
a
4 O# eons
a oan
° alias, me tnd
oH aban m0) tnoum 04 Aceto. one
4 E
ee
duthe prosmba ee Kuoush
oid Disaued plasnad
AimdAiims in4 yo [te PNA “Planar
ae pula uy Animal.
foment don he /
cay oy one v7
9 ern a ys Reknovirus
Guat o_o
pe
riche a yet
dot thaniqeut ie
raw “ee 3A mouse Oy a
a tw ashith,
bptantad he Moot
Rirthe to aa
aA
mt
was Se
Rubras ig
ND) uetrepo bactewan Cell
Aatin
AimaAii a q
ims. es -
alortive theel c * ‘4
. a
Ups d i “ai Hikes fpr Sacto ett)
CM
Pump
HO) Partudte / (Miplistix,) Gor planta)
ia oO. of geal Teanga Taw
vi) eAvetion oind dertting of Recombinant host
Cw:
> Reumbinent DNA wily net hh au
Hy boat eh 40 Atconltnau hose cee
176 AimdAiims in> LHun het fue makin we Ae anti bict,
» apddslont un veetvy, thw it Mien0 Uy tut gone ponncts ok tinge sale
wilh expres Weg iw
sniant Gee cu Chany te
ar pe pa St prOleiw.
> whe ObLained sueombinaut host lb Que
as iw Omounk 40 ar new
Po dutlion eeniquts divelops at @ bow:
fob hovel [upstream process]
Pitot plows deel
Triad Lowel [LdowNsTReam process]
bb but +s Bb te dee le
keunno.Loq cu eopmuil. ; Were, & fence
dew oop eck Le Oo Drawaperred
sss ba 9 ) OL CwactoddiN ute#) Pilot plane duet)
Inteunedtate Level
At this Level, cello are oe
~ andi =a oh woeau gue
os dsoreatlow
Ak tha kw, the err of the product LA
alo poleutated.
blomead@r + lo (000k
> provides Qu. cz conttiton
Aima Aims inda euttinn, el ate
matutacned as prypotege
ay mok aeti«x Lo;
61 exponesttral pag
suorard bw VOY
fw dnnge atpad biomnatinr and A pamavan’
asmpitacing, ole A ames
Aim4Aiims.in> Adaltlon 4 paaiowotty es ehe.
4 Quatity sorTio LE
~ Artal tials CH medictne)
kK & Sewn processing are dipgerente