Vitamin C estimation by back titration

Pre-Lab: Find balance chemical reaction of Part A & B before coming to the lab.

~ 0.01M KIO3
~ 1.0 M H2SO4
0.1M sodium thiosulfate
0.2M KI
Vitamin C Tablet

Part A: Standardization

1. Pipette out 10cm3 of 0.01M KIO3 into a 100cm3 conical flask

2. Add 5 cm3 1.0 M H2SO4 with measuring cylinder and there after add 15 cm3 of 0.2M
KI(excess) using a 25cm3 measuring cylinder. The solution will appear a dark yellow-brown
colour due to the formation of iodine.
3. Titrate this solution with the standardized 0.1M sodium thiosulfate solution, until the yellow
brown colour of the iodine changes to pale yellow.
4. Add 2 cm3 of 1% starch indicator using a dropper, then continue adding the sodium
thiosulfate drop wise until the blue / dark brown colour of the starch-iodine complex has
just disappeared.
5. You should perform a rough titration.
6. Repeat steps 1-4 till the difference in three readings is not more than 0.1cm 3
7. Make an appropriate table for data collected

Part B: Determination of Vitamin C in tablet:

1. Weigh accurately given Vitamin C tablet (±0.01gm). Dilute the given tablet to 100 cm 3 in
volumetric flask.
2. Pipette out 10 cm3 diluted Vitamin C into a 100 cm3 conical flask. Add 10 cm3 of 0.01M KIO3.
3. Add 5 cm3 1.0 M H2SO4 and there after add 15 cm3 of 0.2M KI. The solution will appear a
dark yellow-brown colour due to the formation of iodine.
4. Titrate this solution with the standard 0.1M sodium thiosulfate solution, until the yellow
brown colour of the iodine changes to pale yellow.
5. Add about 2 cm3 of 1% starch indicator, then continue adding the sodium thiosulfate drop
wise until the blue / dark brown colour of the starch-iodine complex has just disappeared.
6. You should perform a rough titration.
7. Carry out steps 1-5 three more times till you obtain consistent results.

Using Part A & B calculate amount of Vitamin C & % yield in given Tablet.

You will be graded for Analysis and Evaluation