EFFECT OF AN ACUTE HANDLING STRESS ON HEPATIC OXIDATIVE STATUS

OF EUROPEAN SEA BASS FED DIETS DIFFERING IN LIPID SOURCE AND

NUTRIMU
CARBOHYDRATE CONTENT

Carolina Castro1,2
Aquafeeds
Amalia Peréz-Jiménez2 Sustainable: Plant Stressors
Aquafeeds
feedsuffs rich in eg. Handling,
Filipe Coutinho1,2 carbohydrates and transport,
vegetable oils confinement
Alexandre Diógenes1,2 What is the feed
more suitable to
Geneviève Corraze3 deal with
Non-sustainable: fish stressful
meal and fish oils conditions?
Stéphane Panserat3

Helena Peres2

Aires Oliva-Teles1,2
AIM Assess the impact of an acute handling stress on hepatic oxidative status of European sea bass fed diets differing
in lipid source and carbohydrate content.
1 Department of Biology, Faculty
of Sciences, University of Porto,
Portugal. 13 weeks of feeding Material & Methods
Sampling

Antioxidant parameters
Stress
300L tanks Transport +
2 CIMAR, Centro de Investigação 25.4±0.5°C Filtered sea-water Handling Enzyme activity:
Marinha e Ambiental, Porto, Triplicate groups of 20 (shaking Sampling Glucose 6-phosphate dehydrogenase
Portugal. E. seabass/ tank (IBW 74g) the water) (G6PD) superoxide dismutase (SOD)
4 Fish meal based diets glutathione peroxidase (GPX)
FOCH- FOCH+ (lipids:18% DM): differing catalase (CAT)
in lipid source (fish oil, FO; glutathione reductase (GR)
vegetable oil, VO) and Oxidative damage biomarker:
VOCH- VOCH+
3 INRA UR1067 NUMEA, St-Pée- starch content (0%,CH-; or lipid oxidative damage (LP)
sur-Nivelle, France 20%-CH+)

Results & Discussion CH- CH+

Acknowledgements CH+>CH-, p<0.001 CH+>CH-, p<0.001

800 VO>FO, p=0.025 120 Lipid source x stress, p=0.014 16
GR mU/mg protein

This work was partially supported by

GPX mU/mg protein

non-stress>stress, p=0.022
G6PD mU/mg protein

600 90 12
the FCT (Foundation for Science and
Technology), Portugal (project 400 60 8
PTDC/MAR-BIO/4107/2012) and co- 200 30 4
financed by the European Regional 0 0 0 Nutritional history
Development Fund (ERDF) through FO VO FO VO FO VO FO VO FO VO FO VO did not modulate
the COMPETE - Operational Non-stress Stress Non-stress Stress Non-stress Stress lipid oxidative status
Competitiveness Programme and non-stress>stress, p<0.001 CH+<CH-, p=0.001 of European sea bass
320 20 800 CH+<CH-, p<0.001
LP nmol MDA/g tissue

national funds through FCT –, under CH x lipid source, p=0.0251 non-stress<stress, p=0.002
CAT U/mg protein
SOD U/mg protein

the project “Pest- 15 600

non-stress>stress, p<0.001 under present
240
C/MAR/LA0015/2011”. C.C., A.P-J. 10 stressful condition.
160 400
and F.C. were supported by grants 5
Exposure to handling
80 200
(SFRH/BD/76297/2011; stress increased
0 0
0 oxidative stress in
SFRH/BPD/64684/2009; FO VO FO VO FO VO FO VO
FO VO FO VO
SFRH/BD/86799/2012 respectively) Non-stress Stress fish, and enzymatic
Non-stress Stress Non-stress Stress
from FCT. A.D. supported by the antioxidant
National Counsil of Technological and mechanisms were
Scientific Development (CNPq), Brazil. Antioxidant
LP content and enzymatic antioxidant mechanisms indicate that CH+
defence not enhanced to
groups were less prone to oxidative damage, independently of stress
counterbalance this
conditions.
Stress exposure increased LP levels in all groups, indicating that increased stress
ROS previous feeding with the tested diets had no effect on attenuation of condition.
production
oxidative stress under stressful condition.
Increased LP under stressful conditions suggests that enzymatic antioxidant mechanisms failed
to adjust to the stressful conditions. Indeed, lower enzymes activities were observed in
stressed fish compared to non-stressed fish. Even in VO groups the higher GPX activity
observed under stressful conditions was inefficient against the accumulation of LP.