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Labortory Manual For South African Sugar Factories PDF
Labortory Manual For South African Sugar Factories PDF
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LABORATORY MANUAL
FOR
S O U T H AFRICAN
SUGAR TECHNOLOGISTS' ASSOCIATION
22810617B
Chapter VI Reagents
1 . General 38- 1962
2. Clarifying reagents .. . . .. .. 38 - 1962
a. Lead subacetate solution for general use 38 - 1962
b. Neutral lead acetate solution .. .. 38 - 1962
c. Dry subacetate of lead . . .. .. 38 - 1962
d. Alumina cream (aluminium hydroxide) 40 - 1962
3. Indicators 40 - 1962
a. Indicators for measurement of pH .. 40 - 1962
b. Neutral water for dilution of indicators 40 - 1962
c. Buffer solutions . . .. .. .. 40 - 1962
d. Indicator solutions made up in the
laboratory .. .. .. .. 40 - 1962
4. Standard acid and alkali solutions .. . . 41. - 1962
5. Iodimetry 42 - 1962
a. General 42 - 1962
b. Preparation of N/32 sodium thiosulphate
solution.. .. .. .. .. 4 2 - 1962
c. Standardization of N/32 sodium thiosul-
phate solution . . .. .. .. 42 - 1962
d. Preparation of iodine stock solution,
N / 1 . 6 approx 43-1962
e. Preparation of N/32 iodine solution . . 43 - 1962
f. Standardization of N / 3 2 iodine solution 43 - 1962
6. F o r the determination of sucrose . . . . 43 - 1962
a. Hydrochloric acid solution .. .. 43 — 1962
b. Sodium chloride solution. . .. .. 43 - 1962
7. F o r the determination of reducing sugars .. 43 - 1962
a. Preparation of standard invert sugar
solution . . .. . . 43 - 1962
b. Preparation of Fehling's solution,
Soxhlet's modification .. .. 44 - 1962
c. Standardization of Fehling's solution . . 44 - 1962
d. Preparation of Luff-Schoorl solution . . 44 - 1962
8. Juice preservative . . .. .. .. .. 45 - 1962
9. F o r the determination of calcium and mag-
nesium . . .. .. . . .. .. 45 - 1962
a. Ethylenediaminetetraacetic a c i d . . .. 45 - 1962
b. Standard calcium chloride solution .. 45 - 1962
c. Buffer solution (according to Saunier
and Lemaitre) . . .. .. .. 45 - 1962
d. Eriochrome black T .. .. .. 45 - 1962
e. Murexide . . .. .. .. 45 - 1962
f. Caustic soda solution .. .. .. 46 - 1962
g. Standardization of ethylenediaminetetra-
acetic acid solution . . .. .. 46 - 1962
FIG URES
1 Counterpoise weight .. .. . . . . .. Chapter IV, 2, o
2 Bagasse container .. .. . . . . .. „ V, 3, a, v
3 First expressed juice sampler .. . . . . .. „ V, 4, a, ii
4 Signalling device for first expressed juice sampler .. „ V, 4, b
5 Mixed juice sampler . . .. .. .. .. „ V, 5, c
6 Brix spindle reading .. .. . . . . .. „ VII, 3
7 Apparatus for determination of SO 2 by Monier-
Williams method . . .. .. . . .. „ VII, 7, c, ii
DEFI N I T I O N S
C A L C U L A T I O NS
1. General
Control data are required for the purpose of factory control and as a
basis for cane payment. The former are recorded in daily, weekly,
monthly and annual reports.
Control d a t a are usually calculated from quantities obtained by
direct weighing (infrequently by direct measuring) in conjunction with
figures obtained by direct analysis.
Quantities obtained by direct weighing a r e : tons* of cane, tons of
imbibition water, tons of mixed juice, tons of filter cake, tons of final
molasses and tons of sugar. T h e m e t h o d s of weighing are described in
chapter II.
2. Calculation of quantities in stock
F o r the purpose of correctly ascertaining sucrose, pol, brix, non-sucrose
and other balances, and certain efficiency data, account must be taken
of the materials currently being processed.
The estimation of the quantities in process is called stocktaking.
In stocktaking a list should be made of the estimated quantities of the
various products and their percentages of sucrose and brix. F r o m these
data the total weights of sucrose and brix in stock and the average
purity of the products in stock are calculated. F r o m the purity figures,
the fraction of sucrose in stock which shall be recovered as sugar is cal-
culated using the S.J.M. formula:
S (J—M)
x = 100 x
J (S—M)
where x = recovery of sucrose % sucrose in the primary
product
S = expected purity of the sugar to be produced
J = purity of the primary product
M = expected purity of the final molasses to be pro-
duced which in Natal is taken as equal to the
average purity of the final molasses produced
in the preceding week.
* Wherever the term tons is used it means tons of 2,000 lb. each.
4. R u l e s for r o u n d i n g off
Wherever a decimal place to be discarded is represented by a n u m b e r less
t h a n five, the preceding digit (that is the last to be recorded) shall remain
as it stands; but where the n u m b e r to be discarded is greater t h a n five,
one shall be added to the preceding digit. Where the n u m b e r to be
discarded is exactly five, the preceding digit shall be unaltered if it is
an even number, but if it is an odd n u m b e r one shall be added to it.
5. Standardization of m e t h o d s of calculation
The calculations in this section are given with a view to standardization
of methods. The formulae do not include all the calculations which the
chemist may be required to m a k e in his capacity as statistician. Others
will be found in subsequent chapters of this manual.
In the following formulae, numbers in square brackets [ ] refer to
the n u m b e r of decimal places to which calculations should be made,
numbers in round brackets ( ) refer to the calculations hereunder.
The letter w signifies a quantity obtained by direct weighing or direct
measurement and x denotes a value obtained by direct analysis.
a. Weights
1. T O N S OF BRIX IN CANE [3]
Tons of brix in mixed juice (9) + tons of brix in bagasse (5).
2. TONS OF SUCROSE IN CANE [3]
Tons of sucrose in mixed juice (10) + tons of sucrose in bagasse (6).
3. TONS OF FIBRE IN CANE [3]
Is equal to tons of fibre in bagasse (7).
4. TONS OF BAGASSE [3]
Tons of cane w + tons of imbibition water w — tons of mixed juice w.
5. TONS OF BRIX IN BAGASSE [3]
Tons of bagasse (4) x brix % bagasse (26)
100
* These are general formulae; if quantities in stock are to be taken into account
the procedure mentioned under 2 above should be followed.
39. O V E R A L L R E C O V E R Y [2]
x 100
7. S u c r o s e b a l a n c e s
T h e sucrose balance offers a convenient m e a n s of detailing losses of
sucrose in process of manufacture. T h e use of t w o such balances is
recommended:
Final Bagasse
Filter Cake
Final Molasses
made and
estimated
Sugar made and
estimated
Undetermined
Cane 100.00
SAMPLI NG
1. General
a. Samples are taken in the sugar industry either from distinct quantities
of material like a t a n k of syrup, a lorry load of cane, a bag of
sugar, or from material which is moving past a sampling point.
Examples of the latter are the sampling of juice from a gutter,
syrup from a continuously running sampling cock, etc. If the
distinct quantity of material to be sampled were truly homogeneous,
the composition of the sample would be identical to that of the
material and the size of the sample would be decided only by the
a m o u n t required for the actual analysis. In practice this is rarely,
if ever, the case. Hence the sample should be composited from a
large n u m b e r of primary samples taken at various spots in the mass
to be sampled. T h e larger the n u m b e r of these primary samples,
the closer the composition of the composite sample will be to the
average composition of the sampled material. If the weight of the
composite sample becomes excessive due to a very large n u m b e r of
primary samples being used in compositing, the composite sample,
after t h o r o u g h mixing, should be sub-sampled. To do this, various
suitable methods are available. Where moving material is being
sampled, sampling is either continuous or semi-continuous. We
speak of continuous sampling when the sample is withdrawn
uninterruptedly and of semi-continuous sampling when the samples
are taken at, usually pre-determined, time intervals. Sampling
sugar by taking a primary sample from each or every fifth bag
which is filled, is semi-continuous. Sampling of final bagasse by
taking a primary sample once every quarter of an h o u r can also
be called semi-continuous, but due to the relatively large time
interval between two primary samples, one could also speak of
snap samples being taken.
The particular m e t h o d of sampling to be employed in each
separate case should be decided on very carefully. It should be
remembered that the aim is to produce a sample, the composition
of which is as similar to that of the mass of the material which is
being sampled as is required by the use of the analytical d a t a
determined on the sample. F o r this reason where great accuracy is
not required, catch samples taken at fairly large time intervals will
often be adequate, particularly when the variability in the c o m p o -
sition of the material to be sampled is relatively small.
2. Cane
a. The very nature of cane makes representative sampling a precarious
undertaking and special care is required to achieve some measure
of accuracy.
To sample a field of cane for the purpose of studying changes
in its sucrose content as an indication of the ripening process,
or for other purposes, in each sampling operation one stalk should
be taken from a large number of marked stools randomly distributed
over the field. If a second sample has to be taken, it too should
consist of one stalk from each of the marked stools used for the
first sampling operation.
b. If a fairly large sample has to be sub-sampled to obtain a quantity
sufficiently small to be crushed conveniently in a laboratory mill,
the following procedure should be used. Sticks are arranged in
descending order of length with tops all one way. Each stick is
then cut into three (approximately equal) lengths and replaced in
position. Sub-sampling is done by taking the t o p sections from the
first, fourth, seventh, etc., the middle sections from the second,
fifth, eighth, etc., and the butt sections from the third, sixth, ninth,
FIGURE 2
BAGASSE CONTAINER
* An illustration of the trier will be found in Spencer and Meade, Cane Sugar
Handbook, New York, John Wiley and Sons, Inc., 8th edition, p. 506.
REAGENTS
1. General
In addition to the name and strength of the reagent, the date of its
preparation should be shown on the label. Solutions should be m a d e
in distilled or deionized water. Volumes should be completed at a
temperature as near 20°C as circumstances will allow.
2. Clarifying reagents
a. Lead subacetate solution for general use: Lead subacetate solution
of 30° Be is prepared by dissolving dry subacetate of lead powder
in hot distilled water and subsequently diluting to 30° Be (54° Bx,
1.25 S.G.).
N o t e : Bottles containing solutions of lead subacetate should always be
provided with a soda-lime tube as a protection against the carbon dioxide of
the atmosphere.
b. Neutral lead acetate solution: Dissolve neutral lead acetate crystals
in distilled water to m a k e a saturated solution, then add glacial
acetic acid to render the solution faintly acid to litmus paper.
Dilute to 30° Be (54° Bx, 1.25 S.G.).
c. Dry subacetate of lead
i. The salt produced shall be dry and conform to the following
specifications of the American Chemical Society which,
together with the methods of testing, have been taken from
I. & E.C., Anal. Ed., 16 (1944) 282.
Basic lead (PbO): not less than 33 %
Insoluble in acetic acid: not more than 0.05 %
Insoluble in water: not more than 2 . 0 %
Moisture (loss at 100°C): not more than 1.5%
Chloride (CI): not more than 0.005 %
Nitrate ( N 0 3 ) : to pass test (limit about 0.003 %)
Substances not precipitated
by hydrogen sulphide: not more than 0.30%
Copper (Cu): not more than 0.005 %
I r o n ( F e ) : not more than 0.005 %
The salt must also conform to the following specifications for
fineness:
1. 100% to pass through a sieve with aperture 0.42 mm
(35-mesh Tyler sieve).
2. 7 0 % to pass through a sieve with aperture 0.125 mm
(115-mesh Tyler sieve).
3. Indicators
a. Indicators for measurement of pH: A complete list of indicators
with directions for their preparation is given in Browne and Zerban,
Sugar Analysis, New York, J o h n Wiley & Sons, Inc. (3rd edition),
p. 559. While it is agreed that indicators can be successfully employed
in chemical control when proper precautions are taken, it is never-
theless recommended that use be made, wherever possible, of pH
meters of which reliable models are available. T h e use of indicator
paper for pH control in the factory is not recommended since such
paper has often been found to yield erroneous results.
b. Neutral water for dilution of indicators: Distilled water which has
been boiled for about 5 minutes and cooled in a flask fitted with
a soda-lime tube, if continuously protected from the atmosphere,
will read 7.0 pH (very nearly) and will constitute a suitable un-
buffered water for dilution.
c. Buffer solutions: Ranges of buffered solutions may be purchased.
The use of these commercial buffers is recommended, but chemists
who prefer to make their own buffer standards are referred to
Browne and Zerban, Sugar Analysis, New York, J o h n Wiley &
Sons, Inc. (3rd edition), p. 556.
It may be recorded that the pH of a 1 % solution of
a m m o n i u m acetate is very nearly equal to 7.0 and again that a
solution of 1.02 g of potassium hydrogen phthalate crystals in
100 ml of distilled water has a constant pH of 4.0 between 18° and
40°C. These buffer solutions will be found suitable for readjusting
pH-meters.
5. lodimetry
a. General
A standardized iodine solution is generally used for measuring the
a m o u n t of what is usually termed " S O 2 in juice". Since one litre
of N/32 iodine solution corresponds to 1 g of S 0 2 , it is convenient
to titrate the juice with an iodine solution of this concentration.
The (unstable) N / 3 2 iodine solution is prepared from N/1.6
iodine stock solution and at least once per 24 hours titrated against
N / 3 2 sodium thiosulphate solution. The thiosulphate solution
should be standardized against potassium iodate at least twice per
month.
b. Preparation of N/32 sodium thiosulphate solution
i. A solution of sodium thiosulphate prepared from the pure
salt in pure water (conductivity water) is quite stable. Slow
decomposition, however, usually occurs, partly due to an
excess of carbon dioxide in the water, partly as a result of the
growth of micro-organisms. Thiosulphate solutions therefore
must be standardized twice per m o n t h ,
ii. N/32 sodium thiosulphate solution contains 7.753 g/litre
N a 2 S 2 0 3 . 5 H 2 0 , but it is not possible to prepare sodium
thiosulphate solutions of definite strength by weighing out the
pure salt as purchased because sodium thiosulphate penta-
hydrate effloresces very easily,
iii. Dissolve 7.75 g of the pentahydrate in 1 litre of freshly boiled,
cooled water, and add 0.1 g of sodium carbonate to the
solution in order to retard deterioration.
c. Standardization of N/32 sodium thiosulphate solution
Potassium iodate reacts with potassium iodide in acid solution to
liberate iodine which can be titrated with thiosulphate:
K I 0 3 + 5KI + 3 H 2 S 0 4 = 3 K 2 S 0 4 + 3I 2 -+- 3 H 2 0
Potassium iodate has an equivalent weight of 35.67 and the A . R .
salt has a purity of 99.9 per cent. It should be dried at 120°C
before use. Weigh out accurately about 1.2 g of pure potassium
FIGURE 6
BRIX
SPINDLE READING
7. Sulphur dioxide
M e t h o d s a. and b. given below yield results which are frequently higher
t h a n the true concentrations of sulphur dioxide present due to the presence
of some other reducing substances in the samples being analysed. However
these methods are sufficiently accurate for factory control purposes.
W h e n highly accurate analyses are required, method c, the Monier-
Williams method, should be employed.
a. The iodine titration method: This m e t h o d is applied especially
for the determination of S 0 2 in juices. The specified volume of
juice (see chapter VIII, 4, e) or any other solution is diluted with the
appropriate volume of water and titrated against N / 3 2 iodine
solution, using a few drops of starch indicator to determine the
end-point. The end-point m a y be m a d e sharper by adding a few
drops of concentrated hydrochloric acid when near the end of the
titration.
b. The rapid method of determining available S 0 2 in sugars: Available
sulphur dioxide m a y be determined rapidly in sugars by titration
with an iodine solution. 100 g of the sugar is dissolved in 150 ml
distilled water in a 500 ml Erlenmeyer flask. 5 ml of approximately
6 N sulphuric acid is added followed by a measured a m o u n t of
N / 3 2 iodine solution r u n in from a burette until there is a slight
excess of iodine. A d d 1 ml of starch solution a n d back-titrate
the excess iodine with N / 3 2 thiosulphate solution until the colour
due to the starch iodine complex j u s t vanishes. T h e available
S 0 2 content of the sugar in parts per million = 10 x (number of
ml N / 3 2 iodine used — n u m b e r of ml N / 3 2 thiosulphate used).
FIGURE 7
A P P A R A T U S FOR D E T E R M I N A T I O N O F SO2 B Y M O N I E R - W I L L I A M S M E T H O D
ANALYSIS OF PRODUCTS
b. Fibre % cane
i. GENERAL FACTORY METHOD: The fibre percentage of
a quantity of cane crushed in a period which begins a n d ends
with an empty mill train is determined by substituting the
relevant data in the expression,
Fibre % cane =
90 Excellent
85 Satisfactory
80 M i n i m u m requirement
x 100.
X
5. Mixed juice
a. Brix: As described in chapter VII, 3.
b. Pol: The pol of mixed juice should normally be determined using
the method given in chapter VII, 4, a. Sometimes, for example,
when juice contains much suspended matter (clay) it is advisable to
use the method given under chapter VII, 4, b.
c. Clerget sucrose by the Jackson and Gillis method IV
i. The cooled sample of mixed juice is brought into the
laboratory and thoroughly mixed. T h e density of the hourly
sample is determined in the usual m a n n e r with a brix hydro-
meter. A sub-sample from each hourly sample is added to a
wide-mouthed glass-stoppered bottle. The weight of each
sub-sample must always be in the same p r o p o r t i o n to the
weight of juice recorded by the factory mixed juice scales
during the period in which the sample was collected. D r y
basic lead acetate is added to the composite sample with
every sub-sample at the rate of 1 g per 100 ml of juice. T h e
a m o u n t of sub-sample and the weight of basic lead acetate
Sucrose
i. THE JACKSON & GILLIS METHOD IV: Weigh out
32.5 g of molasses. Dissolve and make up to the m a r k with
water in a 250 ml flask. Part of this solution is used for the
determination of reducing sugars as described in 10, d below.
To the remainder add sufficient dry lead sub-acetate for clari-
fication (usually about 6 g), shake well and allow to stand for
a few minutes before filtering. To the filtrate add the m i n i m u m
of anhydrous potassium oxalate necessary for de-leading
(usually about 4 g), shake well and again filter. N o w pipette
50 ml portions into 100 ml flasks and proceed with either
method a or b of chapter VII, 5. The readings are made in
200 mm tubes. If the solution is very dark, add a pinch of
zinc dust for decolourizing.
d. Sulphur dioxide: F o r raw and refined sugars either the rapid method
or the Monier-Williams method is used—see chapter VII, 7, b
and 7, c.
e. Moisture: F o r white sugars and raw or similar type sugars take 25
and 10 g of sample respectively. Accurately weigh the sample of
the sugar into a polished aluminium dish provided with a tight-
fitting cover. The dish shall be 8 cm in diameter, 1.1 cm in height
a n d m a d e of 24-gauge aluminium sheet. D r y in an oven at 105°C
for 3 hours. Replace the cover of the dish, cool in a desiccator
and weigh.
f. Sulphated ash: See chapter VII, 13.
g. Conductometric ash {for mill white and refined sugars): The gravi-
metric determination of bisulphated ash on a refined sugar
containing less than 0 . 0 3 % ash requires the use of a precision
U S.G.S. U S.G.S.
mm
F o r practical use in
sugar analysis
First fraction 4.712 2.122 4.8 2.1
Second „ 7.235 1.382 7.2 1.4
Third „ 10.17 0.983 10.0 1.0
Fourth „ 14.37 0.695 14.3 0.7
Fifth „ 24.47 0.409 25.0 0.4
Sixth „ 48.49 0.206 50.0 0.2
2 5 m l Fehling's
10 m l Fehling's Solution 1 Sol Jtion
ml Sugar
Solution mg deducing Sugars per 100 ml so ution w h e n
Required the concentration of s ucrose (g/100 ml) is:—
Og 0.5g 1g 2g 3g 4g 5g 10g 25g 0g 1g
15 .. 336 335 333 329 325 ! 322 317 307 289 824 817
16 .. 316 314 312 309 305 301 297 288 271 772 766
17 .. 298 296 295 291 287 284 280 271 255 727 722
18 . . 282 280 278 274 271 268 264 256 240 687 682
19 267 265 264 260 257 254 250 243 227 651 846
20 255 253 251 249 248 242 238 231 217 619 614
21 . . 243 241 239 236 233 230 227 220 206 589 585
22 .. 232 230 228 225 222 220 216 210 196 563 559
23 222 220 219 216 213 210 207 200 187 1
539 534
24 213 211 210 207 204 202 198 192 179 517 512
25 ! 205 203 202 198 196 194 190 184 171 496 492
26 197 195 194 191 189 186 183 177 164 477 473
27 .. 190 189 187 184 182 179 176 170 158 460 456
28 184 182 180 178 175 173 170 164 152 444 440
29 .. 178 176 174 171 169 167 165 159 147 428 424
30 172 170 168 166 164 161 159 153 142 414 410
31 . . 166 165 163 161 159 157 154 148 137 401 397
32 . . 161 160 158 156 154 152 149 143 132 389 385
33 . . 157 155 153 151 149 147 145 139 128 377 373
34 . . 152 151 149 147 145 143 140 135 124 366 363
35 . . 148 147 145 143 141 139 136 131 121 356 352
36 . . 144 143 141 139 137 135 133 127 117 346 342
37 .. 140 139 137 135 133 131 129 124 114 337 333
38 .. 137 135 134 131 130 128 126 120 111 328 325
39 .. 133 132 130 128 126 124 122 117 107 320 316
40 . . 130 129 127 125 123 121 119 114 104 312 308
41 .. 127 125 124 122 120 118 116 III 102 304 301
42 . . 124 123 121 119 117 116 114 109 99 297 294
43 .. 121 120 118 116 115 113 111 106 97 290 287
44 .. 119 117 116 114 112 110 108 103 94 284 281
45 . . 116 114 113 111 110 108 106 101 92 278 275
46 .. 114 112 111 109 107 105 104 99 90 272 269
47 . . III NO 108 106 105 103 102 96 88 266 263
48 .. 109 108 106 104 103 101 99 94 86 261 258
49 .. 107 106 104 103 102 100 97 92 84 255 252
50 .. 105 103 102 100 99 98 95 90 82 251 248
Temp. Correc- Temp. Correc- Temp. Correc- Temp. Correc- Temp. Correc-
(°C) tion (°C) tion (°C) tion (°C) tion (°C) tion
15.0 2.50 16.0 2.00 17.0 1.50 18.0 1.00 19.0 0.50
15. 1 2.45 16. 1 1.95 17.1 1.45 18.1 0.95 19.1 0.45
15.2 2.40 16.2 1.90 17.2 1 .40 18.2 0.90 19.2 0.40
15.3 2.35 16.3 1.85 17.3 1.35 18.3 0.85 19.3 0.35
15.4 2.30 16.4 1.80 17.4 1.30 18.4 0.80 19.4 0.30
15.5 2.25 16.5 1.75 17.5 1 .25 18.5 0.75 19.5 0.25
15.6 2.20 16.6 1 .70 17.6 1.20 18.6 0.70 19.6 0.20
15.7 2 . 15 16.7 1.65 17.7 1.15 18.7 0.65 19.7 0.15
15.8 2.10 16.8 1 .60 17.8 i .10 18.8 0.60 19.8 0.10
15.9 2.05 16.9 1.55 17.9 1.05 18.9 0.55 19.9 0.05
20.0 0.00 23.0 1.50 26.0 3.00 29.0 4.50 32.0 6.00
20.1 0.05 23.1 1.55 26. 1 3.05 29.1 4.55 32. 1 6.05
20.2 0.10 23.2 1 .60 26.2 3.10 29.2 4.60 32.2 6.10
20.3 0.15 23.3 1 .65 26.3 3.15 29.3 4.65 32.3 6.15
20.4 0.20 23.4 1.70 26.4 3.20 29.4 4.70 32.4 6.20
20.5 0.25 23.5 1.75 26.5 3.25 29.5 4.75 32.5 6.25
20.6 0.30 23.6 1.80 26.6 3.30 29.6 4.80 32.6 6.30
20.7 0.35 23.7 1.85 26.7 3.35 29.7 4.85 32.7 6.35
20.8 0.40 23.8 1.90 26.8 3.40 29.8 4.90 32.8 6.40
20.9 0.45 23.9 1.95 26.9 3.45 29.9 4.95 32.9 6.45
21.0 0.50 24.0 2.00 27.0 3.50 30.0 5.00 33.0 6.50
21 .1 0.55 24.1 2.05 27.1 3.55 30.1 5.05 33.1 6.55
21.2 0.60 24.2 2.10 27.2 3.60 30.2 5.10 33.2 6.60
21.3 0.65 24.3 2.15 27.3 3.65 30.3 5.15 33.3 6.65
21.4 0.70 24.4 2.20 27.4 3.70 30.4 5.20 33.4 6.70
21.5 0.75 24.5 2.25 27.5 3.75 30.5 5.25 33.5 6.75
21.6 0.80 24.6 2.30 27.6 3.80 30.6 5.30 33.6 6.80
21.7 0.85 24.7 2.35 27.7 3.85 30.7 5.35 33.7 6.85
21 . 8 0.90 24.8 2.40 27.8 3.90 30.8 5.40 33.8 6.90
21.9 0.95 24.9 2.45 27.9 3.95 30.9 5.45 33.9 6.95
22.5 1.25 25.5 2.75 28.5 4.25 31.5 5.75 34.5 7.25
22.6 1.30 25.6 2.80 28.6 4.30 31.6 5.80 34.6 7.30
22.7 1.35 25.7 2.85 28.7 4.35 31.7 5.85 34.7 7.35
22.8 1.40 25.8 2.90 28.8 4.40 31.8 5.90 34.8 7.40
22.9 1.45 25.9 2.95 28.9 4.45 31.9 5.95 34.9 7.45
23.0 1 .50 26.0 3.00 29.0 4.50 32.0 6.00 35.0 7.50
T A B L E VI (continued)
First expressed juice Continuous 1 hour 4-hour sample to be pre- Pol Pol
served in mill lab. Brix