Background

Liver cancer is a major leading cancer cause worldwide. HBV may cause liver cancer
by altering certain gene’s expression. Previous research showed there are three HBV
integration hot spots in human chromosome. Thus, we are interested in identifying the
hot spots and their relationship with liver carcinogenesis.

Experimental Design

First, we will use a previously identified tumor sample with the HBV integration site
at MLL4 to measure the gene’s expression. Next, we will conduct qPCR to find out
which tumor samples show high MLL4 expression to become potential candidates for
HBV integration at MLL4. Then, we will design primers to confirm the junction sites.

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