JOURNAL OF EXPERIMENT

Title of Experiment : Fermentation

Day / Date of Experiment : Monday / 7th October 2019
Name : Rika Rahayu Rustam
ID : 1713442002
Class / Group : ICP of Chemistry Education / VI (six)
Members : 1. Kasriani
2. Nurul Annisa
3. Rulmadhani Annisa
4. St. Aminah
Assistant : Yustika Ayu Lestari Rasyid

A. OBJECTIVE OF EXPERIMENT
To study the ability fermentaion amylum, glucose, fructose, mannose,
galactose by some kind of inoculum pure yeast bread (Saccharomyces cerevisiae)
yeast tape and inoculum pure Rhizopus oligosporus.
B. APPARATUS AND CHEMICAL
1. Apparatus
a. Plate 2 pieces
b. Drop pipette 7 pieces
c. Test tube 15 pieces
d. Spoon 1 piece
e. Rack test tube 2 pieces
f. Rod stirrer 1 piece
g. Rough cloth 1 piece
h. Smooth cloth 1 piece
2. Chemical
a. Bread yeast
b. Tape yeast
c. Tempe yeast
d. Amylum 1%
e. Glucose
f. Fructose 5%
g. Mannose 5%
h. Galactose 5%
i. Iodine 0.1%
j. Reagent Benedict/ Fehling
k. Reagent tollens
l. Aquadest
m. Tissue
C. WORK PROCEDURE
1. Starch Hydrolysis Test

yeastragi
suspensi suspension
tape tape, suspensi ragi and
bread yeast roti tempeh yeast
suspensi ragi tempe

25 ml aquadest
25 mL aquadest 2525mL
ml aquadest
aquadest 25 mL25 ml aquadest
aquadest

1 ml of amylum 1%
added into each plate

1 ml of bread yeast suspension is added into

plate 2,3,4. 1 ml of tape yeast suspension is
added into plate 5,6,7. 1 ml of tempe yeast
suspension is added into plate 8,9,10. 1 ml
of aquadest is added inoi plate 1

2. Alcohol
Fermentation
After 5 minutes, 1 drop of iod 0,1% added
inti plate 2, 5 and 6. And so on
 1 2 11 12 14 15
7 8 9 10 13
3 4 5 6

Masukkan Masukkan
PutMasukkan
in 5-20 mL Put5-20
in 5-20
ml mL Put5-20
in 5-20 Put Masukkan
in 5-20 mL Masukkan
Put in 5-20 mL
5-20 ml pati ml mL 5-20 ml 5-20 ml
amylum 1%1% glucose
glukosa fructose
fruktosa galactose mannose
galaktosa mannosa

1 2 10 1 1 1 1 15
3 4 5 6 7 8 9
1 2 3 4

semuaalltabung
tubes areditutup
covered with cotton kapas
dengan

11 14 6 9 12 15
4 7 10 13 2 5 8 3
1

isi 1 ml isi 1 ml
isi 1 ml suspensi suspensi
suspensi ragi tape ragi tempe
ragi roti

Fill 1 mL of tape Fill 1 mL of tempeh

Fill 1 mL of bread yeast suspension
yeast suspension
yeast suspension
 10 12 13 14 15
4 5 6 7 8 9 11

semua digoyang-goyangkan kemudian

all the tubes are shaken periksa
then check thegas karbon
carbon dioksida-nya
dioxide gas

10 12 13 14 15
4 5 6 7 8 9 11

periksa bau alkohol, dengan mencium mulut tabung

Check the smell of alcohol by the mouth of the tube

a. Benedict / fehling test in tubes 1,2 and 3

1 2 3

1 2 3

panaskan beberapa
tetesi reagen
Dropped with benedict or Heat menit
a few minutes Record the changes that
benedict
fehlingfehling
atau reagents occur

b. Tollens test in tabes 1,2, and 3

 1 2 3

1 2 3

panaskan
Heat a fewbeberapa
minutes
tetesi reagen
Dropped with tollens reagents
tollens
menit

----------------
----------------

Record the changes that

occur

Makassar, September 2019

Assistant Practitioner

Yustika Ayu Lestari Rasyid Rika Rahayu Rustam

ID. 1513140007 ID. 1713442002