Reference Wavelength Quantification Typical Low Typical High

Parameter Reagents
EPA AFNOR/ISO (nm) Limit (mg/L) Range (mg/L) Range (mg/L)

Nitrite (as N) TON3 [ N-(naphty)léthylène diamine ] 550 0.03 mg/L ± 0.002 0.03 - 3 mg/L
Cadmium

Nitrite (as NO2) TON3 [ N-(naphty)léthylène diamine ] 550 0.1 mg/L ± 0.007 0.1 - 9.9 mg/L

Nitrate (as N) IMI / TON3 / Cd 550 0.5 mg/L ± 0.0 0.5 - 20 mg/L
[ Imidazole / N-(naphty)léthylène diamine ]

Nitrate (as NO3) IMI / TON3 / Cd 550 2.2 mg/L ± 0.0 2.2 - 89 mg/L
[ Imidazole / N-(naphty)léthylène diamine ]

Phosphate (as P) PO4A / PO4B 880 0.1 mg/L ± 0.000 0.1 - 5 mg/L
[ Molybdate / Tartrate / Ascorbic Acid ]

Phosphate (as PO4) PO4A / PO4B 880 0.25 mg/L ± 0.000 0.25 - 15.4 mg/L
[ Molybdate / Tartrate / Ascorbic Acid ]

Fluoride (as F) ISO 17951 FLUOR A / FLUOR B / FLUOR C 630 0.1 mg/L ± 0.0071 0.1 - 1.5 mg/L
Adapté (CFA)

Nitrate (as NO3) 550 0.6 mg/L ± 0.0416 0.6 - 15 mg/L

Hydrazine

Nitrate (as N) 550 0.1 mg/L ± 0.009 0.1 -3.4 mg/L

Hydrazine
 Reference Wavelength Quantification Typical Low Typical High
Parameter Reagents (nm) Limit (mg/L) Range (mg/L) Range (mg/L)
EPA AFNOR/ISO

Aldehyde - - MBTH / Ferric Cholride 630 0.02 0.02 - 1 -

W-E1-13 SC200 310.2 Adapted from 9963-2 Methyl Orange / Potassium Hydrogenophtalate 550 30 30-300

Alkalinity (as CaCO3) EPA 310.2 - Methyl Orange / Potassium Hydrogenophtalate 550 10 10 - 200 10 - 200

Alkalinity (as CaCO3) - - Bromophenol Blue 590 10 10 - 200 10 - 200

Aluminium - - Phenanthroline / Eriochrome R / Cetyl trimethyl ammonium 420 0.03 0.03 - 0.5 0.1 - 1

Ammonia (as N) 350.1 NF ISO 15923-1 Salicylate / Nitroprusside / Dichloroisocyanurate 660 0.010 0.01 - 1 1 - 100
Annex B

Ammonia (as N) 350.1 - Phenolate / Hypochlorite / Nitroprusside 660 0.010 0.01 - 1 1 - 100

Ammonia (as N) - - Nessler Reagent 420 0.010 0.01 - 1 0 - 40

Boron (as B) EPA-NERL 212.3 NF X 31-122 Azomethine H / EDTA 420 0.5 0,5-10 0 - 10

Bromide (as Br) 4500 NO ISO in colorimetry Chloramine T / Sodium thiosyulfate / Phenol sulfonephtalein 590 0.3 0,3-10

Calcium 215.2 - Arsenazo III 630 1 1 - 25 0 - 100

CEC - - Salicylate / Nitroprusside / Dichloroisocyanrate 660 0.5 me/100g 0.5 - 30 me/100g -

Bromide 4500 - Red Phenol / Chloramine T 590 0.13 0 -1 0-4

Chloride 325.2 NF ISO 15923-1 Ferrous Nitrate / Mercury Thiocyanate / Ascorbic Acid / 480 0.1 0,1-3 1-100
Annex E Antimony Tartrate

Chromium VI 7196-A ISO 23913 Sulfuric Acid / Diphenylcarbazide 550 2 0,002-0,1 0,01-1

COD 410.4 NF EN 1484 (T90-102) Dichromate digestion 570 3 3 - 500 3 - 500

Color Index - ISO 2211 Potassium chloroplatinate / Cobalt Chloride 420 5° 5° -

 Copper - - Bathocuproine 550 0.05 0-5 -

Fluoride 340.1 / 340.3 ISO 17951-2 Alizarin / Lanthanum Nitrate 620 0.1 0,1-1,5 0 - 1.5

Formic Acid EPA with LC K-FORM 340 0.01 0,01-0,1

Formaldehyde Adapted from Ammonium acetate / Ammonium hydroxide / Acetyl acetone 420 5 5-50 10-100
ISO 14184

Adapted from Hydrogenophosphate / EDTA / Sulfate de N,N

Free Chlorine - 520 0.25 0,25-5 1 - 50
ISO 7393-2 DiethylPhenylene-1,4-Diamine DPD

Hardness (as CaCO3) 130.1 - EDTA / Calmagite 520 20 20 - 500 20 - 500

Hydrazine (as N2H4) EPA en LC-MS² Pas d'ISO p-Dimethylaminobenzaldehyde / Methanol / Hydrochloride 460 0.01 0,01-0,5
ASTM D 1385 – 07 acid

ISO with Phenantroline Ascorbic acid / Ferrozine / Acetic acid / Ammonia solution /
Iron EPA with SAA not Ferrozine Hydrochloric acid 550 0.05 0,05-1 1-65

Magnesium - - Xylidil Blue 510 0.7 0 - 15 0 - 50

Manganese EPA with SAA Adapted from Ascorbic acid / Hydroxylammonia chloride / Formaldehyde 480 0.05 0,05-1 0.1 - 10
ISO 6333:1986 solution / Ammonia chloride / Ammonia solution / EDTA

Molybdenum - - Stannous Chloride / Thiocyanate / Sulfo-Nitric Acid 480 0.01 0.01 - 1 -

Nitrite (as N) 354.1 NF EN ISO 13395 (T90-012) Sulphanilamide / N-Naphtyl Ethylene Diamine 550 0.01 0,01-1 0 - 15
NF ISO 15923-1

Phenol Index 420.1 ISO 14402 (T90-127) 4-Amino antipryrine / Ferricyanide Alkalin 520 0.010 0.01 - 0.5 -

NF EN ISO 15681 (T90-083-2)

Phosphates (as P) 365.1 Molybdate / Tartrate / Ascorbic Acid 880 0.05 0,05-2 1 - 50
NF ISO 15923-1

Silicates (as Si) 370.1 NF EN ISO 16264 (T90-053) Molybdate / Oxalic Acid 660 0.4 0,4-40
NF ISO 15923-1

Sulfates 375.4 ISO 22743 Barium Chloride 420 1 1-10 0 - 40

NF ISO 15923-1

Sulfide 376.2 - Dimethyl-p-phenylenediamine / Ferric Chloride 660 0.1 0.1 - 10 -

TKN 351.2 - Salycylate / Nitroprusside / Dichloroisocyanrate 660 0.1 0.1 - 2 1 - 50

 TON (as N) 353.1 NF ISO 15923-1 Hydrazine / Sulphanilamide / N-Naphtyl Ethylene Diamine 550 0.01 0 - 2.5 1 - 25

TON (as N) - Cd 353.2 NF EN ISO 13395 (T90-012) Sulphanilamide / N-Naphtyl Ethylene Diamine / Imidazole 550 0.01 0.01 - 1 1 - 50

TON (as N) - Vanadium - - Vanadium / Sulphanilamide / N-Naphtyl Ethylene Diamine 550 0.05 0.05 - 1 0.8 - 50

Total Cyanides 335.4 - Pyridine / Chloramine T / Barbituric Acid 620 0.005 0.005 - 0.5 -

Total Iron - - Ferrozine 550 0.02 0 - 0.500 0-1

Total Phosphorus 365.4 NF EN ISO 15681-2 (T90-083-2) Molybdate / Tartrate / Ascorbic Acid 880 0.01 0.01 - 1 1 - 50

Urea - - Enzymatic Reaction 340 0.5 0.5 - 10 0 - 1000

Zinc - - Boric Acid / Zincon / EDTA 630 0.13 0.13 - 2 0 - 10

 Reference Wavelength Quantification Typical Low Typical High
Parameter Reagents
ISO (nm) Limit (mg/L) Range (mg/L) Range (mg/L)

Ammonia (as N) 7150* Citrate / Phenol / Nitroprusside 660 0.010 0.01 - 1 1 - 100
adaptée

Boron Azomethine H / EDTA 420 0.01 0.01 - 1.5 -

Calcium Arsenazo III 630 1 1 - 25 -

Bromide Red Phenol / Chloramine T 590 0.13 0 -1 0-4

Chromium VI Sulfuric Acid / Diphenylcarbazide 550 0.002 0 - 0.200 0 - 0.200

COD Dichromate digestion 570 3 3 - 500 3 - 500

Copper Bathocuproine 550 0.05 0-5 -

Fluoride Alizarin / Lanthanum Nitrate 620 0.07 0.070 - 0.700 0 - 1.5

Magnesium Xylidil Blue 510 0.7 0 - 15 0 - 50

Manganese Formaldoxime 480 0.01 0.01 - 1 0.1 - 10

Molybdenum Stannous Chloride / Thiocyanate / Sulfo-Nitric Acid 480 0.01 0.01 - 1 -

Nitrite (as N) 13395* Sulphanilamide / N-Naphtyl Ethylene Diamine 550 0.0005 0 - 0.040 0 - 0.500
adaptée

Phenol Index 4-Amino antipryrine / Ferricyanide Alkalin 520 0.010 0.01 - 0.5 -

Phosphates (as P) 6878* Molybdate / Tartrate / Ascorbic Acid 880 0.005 0.005 - 1 1 - 50
Adaptée

Silicates (as Si) 16264 (CFA)* Molybdate / Oxalic Acid 660 0.003 0 - 0.700 0 -10
adaptée
 Sulfates Barium Chloride 420 0.4 0 - 20 0 - 40

Sulfide Dimethyl-p-phenylenediamine / Ferric Chloride 660 0.1 0.1 - 10 -

TKN Salycylate / Nitroprusside / Dichloroisocyanrate 660 0.1 0.1 - 2 1 - 50

TON (as N) 13395* Hydrazine / Sulphanilamide / N-Naphtyl Ethylene Diamine 550 0.02 0 - 2.5 1 - 25
adaptée

TON (as N) - Cd Sulphanilamide / N-Naphtyl Ethylene Diamine / Imidazole 550 0.01 0.01 - 1 1 - 50

TON (as N) - Vanadium Vanadium / Sulphanilamide / N-Naphtyl Ethylene Diamine 550 0.05 0.05 - 1 0.8 - 50

Total Cyanides Pyridine / Chloramine T / Barbituric Acid 620 0.005 0.005 - 0.5 -

Total Iron Ferrozine 550 0.02 0 - 0.500 0-1

Total Phosphorus Molybdate / Tartrate / Ascorbic Acid 880 0.01 0.01 - 1 1 - 50

Urea Enzymatic Reaction 340 0.5 0.5 - 10 -

 Parameter Reagents Wavelength Quantification Typical Low Typical High
AFNOR/ISO (nm) Limit (mg/L) Range (mg/L) Range (mg/L)

Aluminium - Phenanthroline / Eriochrome R / Cetyl trimethyl ammonium 420 0.03 0.03 - 0.5 0.1 - 1

Ammonia (as N) NF EN ISO 14256-2 Salicylate / Nitroprusside / Dichloroisocyanurate 660 0.010 0.01 - 1 1 - 100

Ammonia (as N) - Phenolate / Hypochlorite / Nitroprusside 660 0.010 0.01 - 1 1 - 100

Ammonia (as N) - Nessler Reagent 420 0.010 0.01 - 1 0 - 40

Boron NF X 31-122 Azomethine H / EDTA 420 0.01 0.01 - 1.5 0 - 10

Calcium - Arsenazo III 630 1 1 - 25 0 - 100

CEC ISO 11260 Ammonium AcetateSalicylate / Nitroprusside / 660 0.5 me/100g 0.5 - 30 me/100g -
Dichloroisocyanrate

Chloride - Ferrous Nitrate / Mercury Thiocyanate / Ascorbic Acid / 520 0.75 0.75 - 50 10 - 250
Antimony Tartrate

Chromium VI - Sulfuric Acid / Diphenylcarbazide 550 0.002 0 - 0.200 0 - 0.200

Copper - Bathocuproine 550 0.05 0-5 -

Fluoride - Alizarin / Lanthanum Nitrate 620 0.07 0.070 - 0.700 0 - 1.5

Magnesium - Xylidil Blue 510 0.7 0 - 15 0 - 50

Manganese - Formaldoxime 480 0.01 0.01 - 1 0.1 - 10

Molybdenum - Stannous Chloride / Thiocyanate / Sulfo-Nitric Acid 480 0.01 0.01 - 1 -

Nitrite (as N) NF EN ISO 14256-2 Sulphanilamide / N-Naphtyl Ethylene Diamine 550 0.0005 0 - 0.040 0 - 15
Organic Material (C/N) ISO 14235 Potassium Tartrate / Salicylate / Nitroprusside / 660 C/ 10 N/ C/ 0 - 320 -
Dichloroisocyanurate / Sulfochromic solution 1 N/ 0 - 40

Phenol Index FD X31-144 4-Amino antipryrine / Ferricyanide Alkalin 520 0.010 0.01 - 0.5 -

Phosphates (as P) NF ISO 11263 Molybdate / Tartrate / Ascorbic Acid 880 0.005 0.005 - 1 1 - 50

Silicates (as Si) - Molybdate / Oxalic Acid 660 0.003 0 - 0.700 0 -10

Sulfates - Barium Chloride 420 0.4 0 - 20 0 - 40

TKN ISO 11261 Salycylate / Nitroprusside / Dichloroisocyanrate 660 0.1 0.1 - 2 1 - 50

TON (as N) - Hydrazine / Sulphanilamide / N-Naphtyl Ethylene Diamine 550 0.01 0 - 2.5 1 - 25

TON (as N) - Cd NF EN ISO 14256-2 Sulphanilamide / N-Naphtyl Ethylene Diamine / Imidazole 550 0.01 0.01 - 1 1 - 50

TON (as N) - Vanadium - Vanadium / Sulphanilamide / N-Naphtyl Ethylene Diamine 550 0.05 0.05 - 1 0.8 - 50

Total Cyanides NF EN ISO 17380 Pyridine / Chloramine T / Barbituric Acid 620 0.005 0.005 - 0.5 -

Total Iron - Ferrozine 550 0.02 0 - 0.500 0-1

Total Phosphorus (as - Joret Hebert Method 880 0.01 0.01 - 1 1 - 50

P2O5)

Total Phosphorus (as - Dyer Method 880 0.01 0.01 - 1 1 - 250

P2O5)

Urea (BIURET) EN15479 Potassium Tartrate / Copper Sulfate 550 0.5 0.5 - 10 100 - 1000
 Wavelength Quantification Typical Range
Parameter Reagents (nm) Limit (mg/L) (mg/L)

Acetic Acid Enzym / Acetate Kinase 340 5 5 - 500

Calcium Arsenazo III 570 0.5 0 - 50

Ferrous Nitrate / Mercury Thiocyanate / Ascorbic Acid /

Chloride 520 0.75 0 - 400
Antimony Tartrate

Color Direct Read 420 0.05 Units 0.05 - 500 Units

D-Lactic Acid Enzym / D-Lactate Dehydrogenase 340 6 0 - 600

Flavonoide P-dimethylaminocinnamaldehyde 630 3 3 - 2000

Magnesium Xylidil Blue 550 1 0 - 100

L-Lactic Acid Enzym / L-Lactate Dehydrogenase 340 5 0 - 500

Phosphate (as P) Molybdate / Tartrate / Ascorbic Acid 880 0.5 0 - 50

Polyphenol Carboxymethyl Cellulose / EDTA 590 1.6 0 - 800

Potassium TPB-Na / TCA 570 1 1 - 500

Reducing Sugars 2.6 Dichlorophenol indophenol 520 2.2 Units 2.2 - 950 Units

SO2 Total DTNB 480 0.1 0 - 10

Sulfate Barium Chloride 520 20 20 - 200

 Reference Wavelength Quantification Typical Range
Parameter Reagents
OIV (nm) Limit (mg/L) (mg/L)

Acetaldehyde - Enzym / Aldehyde Dehydrogenase 340 10 0 - 1000

OIV-MA-AS2-05 Potassium Hydrogenophtalate / Hydrochloric acid / Methyl 30 mg/L ± 2.94 30-250 mg/L
Alkalinity (as CaCO3) 550
adapté orange 100 mg/L ± 3.41 100 - 1 000 mg/L

Acetic Acid - Enzym / Acetate Kinase 340 0.2 g/L ± 0.019 0.2 - 1 g/L

Acetaldehyde OIV-MA-AS315-01 340 20 mg/L 20 - 100 mg/L

Alpha aminic nitrogen 340 50 mg/L 50 - 250 mg/L

Ammoniacal nitrogen - Enzyme / Glutamate Dehydrogenase 340 10 0 - 300

Anthocyanins - Hydrochlorix Acid 510 10 0 - 1000

Citric Acid OIV-MA-AS313-09 Enzym / Citrate Lyase 340 0.2 g/L ± 0.0034 0.2 - 1 g/L

CO2 - Enzym / PEP+ / PEP Carboxylase 380 30 0 - 1500

0.1 mg/L ±
0.0130 0.1 - 2 mg/L
Copper - 590 0.5 mg/L ± 0.5 - 4 mg/L
0.0321
Ethanol - Enzym / Alcohol Deydrogenase 340 10 0 - 500

D-Fructose OIV-MA-AS311-02 Enzym / Hexokinease / PGI / G-6-PD 340 50 0 - 8000

D-Glucose OIV-MA-AS311-02 Enzym / Hexokinease / G-6-PD 340 50 0 - 8000

D-Gluconic Acid - Enzym / Gluconate Kinase 340 50 0 - 5000

 D-Lactic acid OIV-MA-AS313-06 340 0.15 g/L 0.15 - 0.6 g/L

Folin Index OIV-AS2-10 Folin Reagent 660 5 0 - 300

Free SO2 590 0 mg/L 0 - 60 mg/L

Glucose-Fructose 340 0.78 g/L 0.78 - 8.01 g/L

Glycerol OIV-MA-AS312-05 Enzym / GlycerolKinase 340 20 0 -1000

Iron - Iron Ferene 590 2.5 mg/L ± 0.0707 2.5 - 20 mg/L

L-Arginine - Enzym / Arginase 340 2 0 - 100

L-Ascobirc Acid - Enzym / Ascorbic Acid Oxidase / MTT 570 2 0 - 100

L-Lactic Acid OIV-MA-AS313-07 Enzym / L-Lactate Dehydrogenase 340 50 0 - 3000

L-Malic Acid OIV-MA-AS313-11 Enzym / L-Malate Dehydrogenase 340 0.12 g/L 0.12 - 3.15 g/L

Optical Density OIV-MA-AS2-O7B Direct Read 420, 520, 620 - -

Potassium 550 150 mg/L 150 - 1 500 mg/L

pH - Colorimetric Measurement 520 - 2.8 - 4.5 pH

Primary Amino Nitrogen - O-Phtaldialdehyde 340 3 0 - 150

SO2 Free - P-Rosaniline 560 1 0 - 100

 SO2 Total - DTNB 410 3 0 - 250

Sorbic Acid - Thiobarbituric Acid 550 5 0 - 300

Succinic Acid - Enzym / Succinic-CoA Synthetase 340 20 0 - 1500

Sucrose - Enzym / b-Fructosidase / Hexokinase 340 50 0 - 8000

Tartaric Acid OIV-MA-AS313-05B Rebelein 480 0.1 g/L 0.1 - 5 g/L

Total Acidity OIV-MA-AS313-01 Colorimetric Titration 420 - 0 - 5000

Total SO2 420 0 mg/L 120 mg/L

Total polyphenol (Folin- 630 300 mg/L 300 -3 000 mg/L

Ciocalteu)

Urea - Enzym / Urease 340 2 0 - 0.1