Bio-Chemistry Analyzer: CS-T240 Operation Manual

CS-T240 Operation Manual
CS-T240
BIO-CHEMISTRY ANALYZER
Instruction:
Dear user, thanks for purchasing our CS-T240 Auto-Chemistry Analyzer.
Please read the user manual carefully in order to operate the instrument correctly. Incorrect operation may
effect the precision and accuracy of the test results, or endanger personal safety.
Please keep the user manual safely for your any time reference.
Note:
● Instrument should be operated by medical inspection specialist, physician, nurse or lab assistant whom are
specially trained.
● Instrument should be controlled by special software. Please install the software that is appointed by our
company. Installation of other software/hardware may interfere normal operation. Don’t operate other software
when instrument operating.
● Dust may accumulate on the surface of instrument after long time storage. Soft cloth or gauze can be used for
cleaning work, and a little detergent can be used if necessary. Please cut off the power supply before cleaning.
When instrument is not used, make sure shut the lid down.
●As to the use and storage method of the sample, reagent, Controls, Calibrator, please refer to the relevant
instructions.
●Sample, Controls, Calibrator and waster solution have the potential biochemical infectivity, the detergents are
corrosive that may hurt eyes, skin and mucosa. Operator should refer to the safety regulation for lab operation.
Protective measure should be taken to operator (Such as lab protective clothes and gloves).
●Avoid contact with eyes and skin, in case of skin contact, flush the area with water, rinse immediately with
plenty of water and seek medical advice.
● Operator should comply with the local regulation when draining and dealing with reagent, waste solution,
waste sample, consumable etc. Please dispose the waste solution and instrument consumable according to the
regulation of medical waste, infective waste and industrial waste.
Warning:
●. Instrument should be operated in a good ground condition, and an independent power supply is a must, the
input power should be conformed to instrument requirement.
●. Don’t pull the electrical wire with wet hand, or there is a risk of electrical shock.
●. Don't stamp, twist, drag the wire and cable, or it may cause a fire.
●. Please don’t open the back and side cover board before cutting the general power supply except DIRUI
special service staff.
●. If liquid occurs in instrument interior or there is an internal pipeline leakage, please immediately cut off the
general power supply, and contact DIRUI customer service dept.
● Please don’t touch sample probe, reagent probe and stirring rod, etc. when instrument operating, don’t put your
hand into the opening part, or it may cause body injury or instrument damage.
● Cut off the power supply before replace light source lamp. Don’t touch the lamp before it is cool to avoid
burning.
● Periodic maintenance should be executed strictly according to the user manual. Or it may cause instrument
malfunction, and effect the accuracy and precision of test results.
● Make sure that the Auto-Chemistry Analyzer is operated according to the user manual, or the measuring result
is not a reliable one, and the damage on instrument may endanger human safety.
● Please don’t place combustible material around the instrument.
Catalogue
Chapter 1 Brief Introduction................................................................................................................................. 7
1.1 Summary........................................................................................................................................................ 7
1.2 Main Technical Index .................................................................................................................................... 7
1.3 Composition of instrument ............................................................................................................................ 8
1.3.1 Front picture ............................................................................................................................................... 8
1.3.2 Back picture ................................................................................................................................................ 9
1.3.3 Leftside picture ........................................................................................................................................... 9
1.4 Configuration and function.......................................................................................................................... 10
1.4.1 Operating system ...................................................................................................................................... 10
1.4.2 Analytical system...................................................................................................................................... 10
1.4.2.1 Sample reagent disk ........................................................................................................... 10
1.4.2.2 Sampling mechanism ......................................................................................................... 11
1.4.2.3 Reaction disk ..................................................................................................................... 13
1.4.2.4 Incubation bath .................................................................................................................. 14
1.4.2.5 Stirring mechanism ............................................................................................................ 14
1.4.2.6 Reaction cuvette rinsing mechanism ................................................................................. 15
1.4.2.7 Reagent cooling system ..................................................................................................... 17
1.4.2.8 Optical system ................................................................................................................... 17
1.5 Instrument Symbol ...................................................................................................................................... 18
Chapter 2 Measuring Principle ........................................................................................................................... 19
2.1 Mechanism movement principle.................................................................................................................. 19
2.1.1 Operation flow .......................................................................................................................................... 19
2.1.1.1Rinsing unit......................................................................................................................... 19
2.1.1.2 Sample and reagent adding unit ......................................................................................... 19
2.1.1.3 Stirring unit ........................................................................................................................ 20
2.1.1.4 Reaction disk unit .............................................................................................................. 20
2.1.2 Metering Characteristics ........................................................................................................................... 22
2.2 Analytical mode ........................................................................................................................................... 22
2.2.1Assay mode variety ................................................................................................................................... 23
2.2.2 Calibration Method................................................................................................................................... 28
2.3 Check of measure ........................................................................................................................................ 42
2.3.1 Calibration check ...................................................................................................................................... 42
2.3.2 Absorbance limit....................................................................................................................................... 43
2.3.3 Linearity Abnormal Check ....................................................................................................................... 44
2.3.4 Prozone check........................................................................................................................................... 45
Chapter 3 Instrument Installation ....................................................................................................................... 47
3.1 Installation requirement ............................................................................................................................... 47
3.1.1 Space Requirement ................................................................................................................................... 47
3.1.2 Environment requirement ......................................................................................................................... 47
3.1.3 Power requirement.................................................................................................................................... 47
3.2 Open package .............................................................................................................................................. 47
3.2.1 Procedure .................................................................................................................................................. 47
3.2.2 Handling method ...................................................................................................................................... 48
3.3 Installation procedure .................................................................................................................................. 48
3.3.1. software installation................................................................................................................................. 48
3.3.2 Peripherial device connection................................................................................................................... 51
3.3.2.1 Connection of pure water inlet pipeline............................................................................. 51
3.3.2.2 Connection of waste liquid outlet pipeline ........................................................................ 51
3.3.2.3 Connection of computer .................................................................................................... 51
3.3.2.4 Printer installation.............................................................................................................. 51
3.3.3 System login ............................................................................................................................................. 51
Chapter 4 Accessory Device............................................................................................................................... 56
4.1 Barcode reader ............................................................................................................................................. 56
4.1.1 Scan range of barcode reader.................................................................................................................... 56
4.1.2 Sample container requirement .................................................................................................................. 56
4.1.3 Barcode using requirement ....................................................................................................................... 56
4.1.4 Stick requirement of sample barcode ....................................................................................................... 57
4.1.5 Reagent bottle requirement：................................................................................................................... 57
4.1.6 Reagent barcode using requirement.......................................................................................................... 57
4.1.7 Reagent barcode stick requirement........................................................................................................... 57
4.1.8 The rule of reagent barcode ...................................................................................................................... 57
4.1.9The using of sample reagent barcode reader ............................................................................................. 59
4.2 Purified water equipment............................................................................................................................. 59
Chapter 5 Software Operation ............................................................................................................................ 60
5.1 Software interface instruction...................................................................................................................... 60
5.1.1 Main interface composition ...................................................................................................................... 60
5.1.2 Keyboard function .................................................................................................................................... 62
5.1.3 Software function frame ........................................................................................................................... 62
5.2 Software Operation...................................................................................................................................... 64
5.2.1 Icon move ................................................................................................................................................. 64
5.2.2 Function key selection .............................................................................................................................. 64
5.2.3Open Form ................................................................................................................................................. 64
5.2.4The operation of list box and scroll bar ..................................................................................................... 65
5.2.5 Pull down menu operation ........................................................................................................................ 66
5.2.6Button box and check box ......................................................................................................................... 66
5.3 Instrument standard specification ................................................................................................................ 66
Chapter 6 Instrument Operation ......................................................................................................................... 68
6.1Operation overview ...................................................................................................................................... 68
6.2Detailed operation......................................................................................................................................... 69
6.2.1 Check before measurement....................................................................................................................... 69
6.2.2 Power on and software login .................................................................................................................... 69
6.2.3Check instrument status ............................................................................................................................. 69
6.2.3.1 Alarm check....................................................................................................................... 69
6.2.3.2 Light quantity check .......................................................................................................... 71
6.2.3.3 Cuvette blank check........................................................................................................... 72
6.2.3.4 Check the temperature of incubation bath ......................................................................... 73
6.2.4 Check analyze condition........................................................................................................................... 73
6.2.4.1 Check analysis conditions of colorimetric item ................................................................. 73
6.2.5 Reagent preparation .................................................................................................................................. 75
6.2.5.1 Reagent usage and important notice .................................................................................. 75
6.2.5.2 Reagent manual registration .............................................................................................. 76
6.2.5.3 Barcode scanning ( automatic registration) ....................................................................... 77
6.2.5.4 Reagent Horizontal ............................................................................................................ 78
6.2.5.5 Delete reagent information ................................................................................................ 79
6.2.6.2 QC item registration .......................................................................................................... 81
6.2.7 Sample registration and testing（Sample registration）.......................................................................... 81
6.2.7.1 Single sample registration.................................................................................................. 81
6.2.7.2 Registration of batch routine sample ................................................................................. 83
6.2.7.3 Edit the patient info ........................................................................................................... 84
6.2.7.4 Data application ................................................................................................................. 86
6.2.7.5 Modification and deletion of sample information ............................................................. 86
6.2.8 Test preparation ........................................................................................................................................ 87
6.2.8.2 Test..................................................................................................................................... 87
6.2.9 Testing process.......................................................................................................................................... 87
6.2.9.1 System monitor .................................................................................................................. 87
6.2.9.2 Emergence stop.................................................................................................................. 90
6.2.9.3 Sample addition ................................................................................................................. 90
6.2.10 Test result checkup (Result data) ............................................................................................................ 91
6.2.10.1 Daily result ...................................................................................................................... 91
6.2.10.2 Check results within three days ....................................................................................... 97
6.2.11 Sample recheck ..................................................................................................................................... 100
6.2.12 Analyze complete ................................................................................................................................. 103
Chapter 7 Calibration Information.................................................................................................................... 105

7.1 Colorimetric calibration............................................................................................................................. 105
7.1.1 Calibration registration for colorimetric items ....................................................................................... 105
7.1.2Calibration result of colorimetric item .................................................................................................... 107
Chapter 8 Quality Control .................................................................................................................................111
8.1 QC registration ...........................................................................................................................................111
8.1.1 QC regulation setup ................................................................................................................................ 112
8.1.2 QC name setup ....................................................................................................................................... 113
8.1.3 QC item registration ............................................................................................................................... 114
8.1.4QC parameter modification ..................................................................................................................... 114
8.1.5 Delete OC item ....................................................................................................................................... 114
8.2QC interval ................................................................................................................................................. 114
8.3 Monthly quality control ............................................................................................................................. 116
Chapter 9 System Setup.................................................................................................................................... 119
9.1 Chemistry parameter.................................................................................................................................. 119
9.1.1 Add/delete item ...................................................................................................................................... 119
9.1.2Analysis parameter .................................................................................................................................. 120
9.1.3 Calibration parameter ............................................................................................................................. 124
9.1.4 Range parameter ..................................................................................................................................... 125
9.2 Item combination ....................................................................................................................................... 127
9.3Calculated itme ........................................................................................................................................... 128
9.4 Cross contamination .................................................................................................................................. 130
9.4.1 Reagent probe cross contamination ........................................................................................................ 130
9.4.2 Reaction cuvette cross contamination .................................................................................................... 131
9.5 Report sheet format ................................................................................................................................... 132
9.5.1 Basic information setup .......................................................................................................................... 132
9.5.2 Print sequence setup ............................................................................................................................... 132
9.5.3 Report printout format setup................................................................................................................... 133
9.5.3.1 Report template setup ...................................................................................................... 133
9.5.3.2 default format setup ......................................................................................................... 134
9.6 Other setup................................................................................................................................................. 134
9.7 Manual item setup ..................................................................................................................................... 136
9.8 LIS communication setup .......................................................................................................................... 137
9.9 Reagent topping up setup .......................................................................................................................... 138
9.10［Reagent+sample］disk setting ............................................................................................................. 139
Chapter 10 System management ...................................................................................................................... 141
10.1 User information...................................................................................................................................... 141
10.2 Hospital information................................................................................................................................ 142
10.2.1 Delivery dept. ....................................................................................................................................... 142
10.2.2 Delivery doctor ..................................................................................................................................... 143
10.3 Other information .................................................................................................................................... 143
10.3.1 Patient type ........................................................................................................................................... 143
10.3.2 Clinic diagnosis .................................................................................................................................... 144
10.3.3 Report remark ....................................................................................................................................... 145
10.3.4 Item unit................................................................................................................................................ 146
10.4Workload statistics.................................................................................................................................... 147
10.5 Database maintenance ............................................................................................................................. 149
10.6 System log ............................................................................................................................................... 150
Chapter 11 System Help ................................................................................................................................... 151
11.1System help application ............................................................................................................................ 151
Chapter 12 System Maintenance ...................................................................................................................... 152
12.1 System maintenance preparation ............................................................................................................. 152
12.1.1 Instrument and tools ............................................................................................................................. 152
12.1.2 Pure water ............................................................................................................................................. 152
12.1.3 Detergent .............................................................................................................................................. 152
12.2 The Application of system maintenance menu ........................................................................................ 153
12.2.1 Reset ..................................................................................................................................................... 153
12.2.2 Cleaning water tank .............................................................................................................................. 153
12.2.3 Light quantity check up ........................................................................................................................ 153
12.2.4 Cell blank check ................................................................................................................................... 154
12.2.5 Air exhaustion of syringe...................................................................................................................... 155
12.2.6 Rinsing /air exhaust detergent pipeline................................................................................................. 156
12.2.7 Rinsing reaction cuvette ....................................................................................................................... 156
12.2.8 Rinsing incubation bath ........................................................................................................................ 156
12.2.9 Sample reagent probe vertical checkup ................................................................................................ 156
12.2.10 Sample reagent probe horizontal checkup .......................................................................................... 156
12.2.11Stirring mechanism horizontal checkup............................................................................................... 156
12.2.12 Mechanism operation checkup ........................................................................................................... 157
12.2.13 Bar code reader checkup..................................................................................................................... 157
12.2.14 Automatically rinse the pipeline of concentrated liquid ..................................................................... 157
12.2.15 Manually rinse concentrated waste liquid pipeline............................................................................. 158
12.3 Maintenance and checkup points and parts ............................................................................................. 159
12.3.1 Periodic cleaning ,checkup and parts replacement ............................................................................... 159
12.3.2 Periodical replacement parts list ........................................................................................................... 160
12.4 Maintenance method................................................................................................................................ 161
12.4.1 Sample reagent probe ........................................................................................................................... 161
12.4.1.1 Daily washing (automatic washing) .............................................................................. 161
12.4.1.2 Cleaning outside of probe tip......................................................................................... 162
12.4.1.3 Cleaning clogged probe ................................................................................................. 162
12.4.1.4 Adjusting probe position................................................................................................ 164
12.4.1.5 Cleaning rinsing bath ..................................................................................................... 165
12.4.2 Reaction disk ........................................................................................................................................ 166
12.4.2.1 The confirmation of the contaminated reaction cuvette................................................. 166
12.4.2.2 Reaction cuvette cleaning .............................................................................................. 167
12.4.2.3 Replace reaction cuvette ................................................................................................ 167
12.4.2.4 Cleaning incubation bath and the drain filter of the incubation bath ............................. 168
12.4.2.5 Liquid level sensor of the incubation bath ..................................................................... 169
12.4.3 Cleaning detergent bottle ...................................................................................................................... 169
12.4.4 Light source lamp ................................................................................................................................. 170
12.4.4.1 Light quantity check ...................................................................................................... 170
12.4.4.2 Replace the light source lamp ........................................................................................ 170
12.4.5 Cleaning the rinsing nozzle .................................................................................................................. 172
12.4.6 Stirring rod............................................................................................................................................ 173
12.4.6.1 Cleaning of the stirring rod ............................................................................................ 173
12.4.6.2 Replacing the stirring rod .............................................................................................. 173
12.4.7 Reagent sample cooling unit................................................................................................................. 175
12.4.8 Syringe pump........................................................................................................................................ 175
Chapter 13 Alarm and Processing .................................................................................................................... 176
13.1 Alarm information type ........................................................................................................................... 176
13.2 Countermeasure to malfunction do not issue alarm................................................................................. 176
13.2.1 Data malfunction which do not issue alarm.......................................................................................... 176
13.2.2 Instrument malfunction which do not issue alarm ................................................................................ 177
13.3 Alarm information content and countermeasure...................................................................................... 178
Chapter 14 Risk Evaluation .............................................................................................................................. 193
Chapter 15 Instrument Transportation and Storage ............................................................................................. 197
15.1 Transportation requirement ..................................................................................................................... 197
15.2 Storage requirement................................................................................................................................. 197
15.3 Storage environment ................................................................................................................................ 197
Addendum A Product Warranty ........................................................................................................................ 197
Addendum B Product Description.................................................................................................................... 198
Statement ............................................................................................................................................................. 203
Chapter 1 Brief Introduction

1.1 Summary
CS-T240 Auto-Chemistry Analyzer is an instrument with discrete system, reagent open function, emergency priority
function as well as an external computer. The instrument is composed of humanized software operation system,
intelligentized optical unit, complicated mechanism system, precision liquid path and accuracy electrical system.
The instrument could automatically realize sampling, reagent injection, anti-interference, mixture, pre-
temperature, reaction measurement, rinse, calculation, display and print function. The substitution of manual
operation for automatic operation could not only enhance the working efficient but also decrease the test error, thus
greatly enhance the accuracy and precision of test results.
CS-T240 Auto-Chemistry Analyzer could carry out the immunology check and biochemical analyze of blood,
urine, ascites, cerebrospinal fluid and other body fluid. The instrument could also carry out clinic test, such as:
myocardium enzymogram, blood sugar, blood fat, liver function, renal function, immunoglobulin, etc.
1.2 Main Technical Index
Instrument structure: Discrete system

Throughput: 200-300 tests/ hour
Simultaneous analysis item No.: At most 60 colorimetric items
Sample volume: 3 to 50μl（Stepping 0.1μl）
Reagent volume: 10 to 450μl（Stepping 1μl）
Reaction solution volume: 150～550μl
Liquid level sensor: Integration of sample reagent probe with touch sensor and probe block test
function.
Stirring: Independent stirring after reagent injecting.
Sample position, reagent position: The reagent and sample share one disk, totally 66 positions. User-defined
proportion of reagent position and sample position
Photometer: Grating spectrophotometry system in a range of 340～750nm, wavelength:
340, 380, 405, 450, 480, 505, 546, 570, 600, 660, 700, 750nm
Wave length accuracy: ±2nm
Light source: 20W /12V Long life quartz halogen lamp ( water cooling) Measurement
range: 0 to 3.3Abs
Reaction disk: 120 pcs of reusable rigid optical plastic reaction cuvette. Reaction cuvette
optical diameter: 6mm
Reaction cuvette rinse: Automatic
Incubation bath temperature: 37℃±0.1℃
Reaction time: 13 minutes
Analysis method: Rate assay ,end-point assay, 2-point assay.
Calibration method: 1-point linearity , 2-point linearity, multi-point linearity, non-linearity
method.
Reagent bottle volume: 20ml, 70ml ,100ml
Reagent cooling unit: All reagents keep at 5℃ - 15℃ or 2～8℃(Optional Refrigeration),
semiconductor refrigeration.
Barcode scanning: 1 internal barcode scanner( scan the barcode on the routine sample and
reagent, scan the barcode of outer track reagent and sample .)
Reagent volume test : Test and report the reagent remaining volume.
Power supply: ~220V 50 Hz
Ambient condition:
──Ambient temperature： 15℃～32℃，suitable temperature：18℃～25℃；
──Relative Humidity： 40%～85%；
Relative humidity: 40%～85% Appearance dimension:
Chemistry Analyzer dimension: 998×752×517mm(length×width×height)；
With cabinet: 998×752×1142mm(length×width×height)；
Output power: 650VA Weight: About 120Kg
1.3 Composition of instrument

1.3.1 Front picture
①cover symbol ②cover ③detergent and detergent sensor ④model

⑤reaction cuvette rinsing unit ⑥reaction disk ⑦stirring unit ⑧probe ⑨reagent sample disk
Figure 1-1 Front of instrument
1.3.2 Back picture
②
③
⑥
④ ⑤
①Syringe ② back nameboard ③purified water injection inlet

④Waste discharge outlet ⑤Bio-hazard identification ⑥RS-232 interface
Figure 1-2 Back of instrument
1.3.3 Leftside picture
①
②
③
④ ⑤
① Cooling Indicator ②Analysis Indicator ③Power Switch ④ Electrical outlet ⑤
Analysis Switch
Figure 1-3 Leftside of instrument

1.4 Configuration and function
CS-T240 Auto-Chemistry Analyzer is composed by operating system and analytical system. The two parts is
connected by RS-232 serial wire.
1.4.1 Operating system
Operating system is composed of mainframe, 17 inch CRT display monitor, keyboard, mouse and printer.
Mainframe: Windows XP system
Special applied software and database.
Computer configuration: CPU basic frequency ≥2.8GHz ， hard disk≥160 G ，
Memory≥1G，with RS-232 serial port、internet port and USB interface
with RS-232 serial interface, website interface and USB interface.
Display monitor: Display all kinds of form, curve and test data of CS-T240 software.
Keyboard : Operation control and data input.
Mouse: Carry out software operation
Printer : Print out test data and chart.
1.4.2 Analytical system
Analytical system is composed of sample reagent disk, sample reagent pipetting mechanism, reagent disk,
stirring mechanism, cooling system, rinsing mechanism, optical system etc.
1.4.2.1 Sample reagent disk
! Warning:
△
Do not touch sample reagent cover when the instrument is running, or it may cause body injury or instrument
damage.
① ② ③ ④ ⑤ ⑥ ⑦
①Sample reagent disk cover ② Disk cover lock knob ③sample tube ④sample reagent
disk handle ⑤ Disk cover detection switch ⑥Inner reagent bottle ⑦Outer reagent bottle
⑧Disk Lock Buckle ⑨Disk-oriented pin
Figure 1-4 Sample reagent disk
(1) Function
Sample reagent disk is used for sample and reagent bottle placing. Place the containers ( standard cup, micro
cup , test tube) which contain calibrator, sample, control on the sample position, and then place the reagent,
CS-anti-bacterial phosphorus-free detergent on the reagent positon, the disk will send them to the sampling
position in the sampling mechanism.
Cooling system provide cooling condition for sample reagent disk to facilitate low-temperature reagent storage.
Refrigerated warehouse with a bar code reader window, and can scan the barcode of outer reagent and sample.
(2) Specifications
The reagent and sample share one disk, totally 66 positions. User-defined proportion of reagent position and
sample position.（The maximum reagent position is 42，the minimum is 6）, no. 45 positon should be
CS-anti-bacterial phosphorus-free detergent
Reagent bottle volume：20ml、70ml、100ml。
Sample cup：standard cup, micro-cup, test tube.
(3) Movement
At Power on: it turns counterclockwise to move No.1 position to the pipetting mechanism sucking positon.
At analysis: At the beginning of analysis, sample disk makes the same movement as ―power on‖. During
analysis, sample disk turns to the direction allowing a quicker access.
At resetting: Make the same movement as at ―power on‖.
(4) Dismounting
The two locking buckle is used for two fix the plate. In dismounting, release the lock buckle fisrst, be sure
to set the position port matching with the guide pin.
Be sure to secure the cooling unit lid on the inner track, The outer track can be demounted without
removing the inner track.
Note: The instrument will issue alarm when the cover is opened under the condition of standby or testing.
Under standby, the instrument will carry out reagent horizontal scan.
(5) Action check
Single-click ― maintenance‖ key, select ― mechanism operation checkup‖, input the check times,
single-click ― Execute ‖ button. If abnormality exits,instrument will issued alarm.
1.4.2.2 Sampling mechanism
! Warning
△
● Make sure that the sample reagent disk cover is well covered when the instrument is running.
① ② ③ ④
① Rinseing bath of pipetting probe ② Pipetting probe

③ Pipetting probe arm ④ Pipetting probe elevating
Figure 1-5 Sample reagent pipetting
(1) Function
Assmilates a specified amount of sample from sample container and a specified amount of reagent from
reagent container, and put them into the reaction cuvette.The pipetting probe is also a liquid level sensor.
Calculate the left reagent volume through the decrease distance of the probe. The left reagent volume will
be displayed in “reagent information‖form.
(2) Specification
Sample setting volume: 3～50ul, set in 0.1ul stepping.
Reagent setting volume: 10～450ul, set in 1ul stepping.
In sample pre-dilution, the specified amount of purified water from the inner wall of pipetting probe will be
added into reaction cuvette.The diluent volume is 10～450ul.
Important Notice: residual reagent volume、remaining tests is calculated upon（Setting amount + residual）.
(3) Movement
At power on: The sample probe comes over above the reaction cuvette, and then returns above the
sample probe rinse trough.
At analysis: The probe moves follow the sequence of sample cup, reagent bottle, reaction cuvette,probe
rinsing bath.
At resetting: Makes the same movement as at power on.
(4) Automatic rinsing
After reagent pipetting, assimilate CS-anti-bacterial phosphorus-free detergent from the 45th position of
sample reagent disk. And pipet them into the reaction cuvette, and then return to pipetting probe washing
tank to wash the inner and outer wall. Adding detergent for 3 times, totally 1.05ml.
(5) Operation check
Single-click the ― System Maintenance‖ key, select ― mechanism operation checkup‖, and input the check
times. Click ―Execute ―. If abnormality exists, instrument will issue alarm.
1.4.2.3 Reaction disk
! Warning:
△
● Please don’t touch the lid of the reaction disk when running, or it may cause body injury and instrument
damage.
① ② ③ ④ ⑤ ⑥
① Reaction cuvette rinsing unit ② Reaction disk ③Reaction disk fixed knob
④Cup holder fixing screw ⑤Guide pin and guide hole ⑥Reaction cup component handle
Figure 1-6 Reaction Disk

(1) Function
Fix the reaction cuvette to the rotating reaction disk with screw, the reaction liquid reacts at 37 ℃ reaction
tank and conduct absorbance measurement in the rotation.
(2) Specifications
Reaction cuvette No.：20/unit×6 unit，totally 120 reaction cuvettes.
Light path ：6mm
Reaction cuvette material：optical plastic
(3) Movement
Usually counter clockwise rotation.
At power on: Rotate, stop at the starting position. No. 1 reaction cuvette is under the first cleaning nozzle.
At analysis: Initial operation is the same as at power on. And then add two reaction cuvettes after one
circle (122 reaction cuvettes). Repeat this action process. It takes about 18 seconds to rotate a
circle
At resetting: Make sure same as at power on.
(4) Reaction cuvette cleaning
Place a anti-bacterial phosphate-free detergent bottle at 45 position of sample reagent disk. Open the reagent
bottle cover and conduct "reaction cuvette cleansing" in the "system maintenance" form, all of the reaction
cuvette can be cleaned. However, due to automatic cleaning by using CS-alkaline detergent in CS-alkaline
detergent box of the working analyzer, everyday maintenance do not needed.
(5) Operation check
Single-click the ― Maintenance‖ key, select ― mechanism operation checkup‖, and input the check times.
Click ― Execute―. If abnormality exists, instrument will issue alarm.
(6)Mounting/ Dismounting
Reaction disk: First remove the reaction cuvette cleaning unit of the reaction disk(top), then screw the
central knob of the reaction cuvette,the reaction disk can be lifted. In the installation, matching the guide
hole with the guide pin of the reaction disk seat, and then tighten the fixed knob.
Reaction cup: remove the screw of the reaction cuvette, grasp the handle of reaction cuvette component
upward, the reaction cuvette can be removed from the reaction disk.
Note: Place the removed the reaction cuvette in pure water to save. In addition, if the analyzer has been
shutdown for at least 3 days, reaction cuvette need to be removed, and placed in pure water.
1.4.2.4 Incubation bath
! Warning:
△
● Keep the cleanness of purified water in incubation bath, or it may effect the test precision.
● When instrument startup or rinsing incubation bath, make sure there is enough CS-anti-bacterial
phosphor-free detergent at No.45 position.
(1) Function
Keep the reaction solution in the reaction cuvette at a constant temperature.
(2) Operation
At power on: Automatic exchanges the constant temperature water once, the CS-anti-bacterial phosphor-free
detergent in position No.45 of both reagent disks is added in incubation bath.
At analysis: Incubation bath water is circulating. Instrument may automatically supply water when water
shortage comes in operation process.
Exchange water: In ―maintenance‖ window, select ―rinsing incubation bath‖, and then the constant
temperature water may exchange, and then add 2.7ml CS anti-bacterial phosphor-free detergent in
incubation bath water.
Note: After running for 24 hours, instrument may require ―incubation bath water exchange‖, please carry
out ―Rinsing incubation bath‖.
1.4.2.5 Stirring mechanism
! Warning:
△
● Please don’t touch stirring mechanism when operate, or it may cause body injury or instrument damage.
② ③
① mixer ② mixer rinsing bath ③mixer arm
Figure 1-7 Reaction disk
(1) Function
Stirring the reaction solution in each reaction cuvette.
(2) Operation
At power on: Move to the side of reaction cuvette and then stops above the rinsing bath, move to the side
of reaction cuvette again, and then stops above the rinsing bath.
At analysis:The mechanism descends, rotates, risees and stops between two locations: reaction cuvette and
stirring rod rinsing bath.
Stirring is carried out after each addition of reagent.
(3) Automatic rinsing
Automatic rinsing of mixer: when mixer descends into mixer rinsing trough, mechanism may automatically
rotates and washes the mixer with purified water.
Sampling finishing: mixer is stirring in reaction cuvette in which detergent is added, thus rinse the mixer.
(4) Operation check
Single-click the ―maintenance‖ key, select ―mechanism operation check‖, and input the check times. Click
―Execute‖. If abnormality exists, instrument will issue alarm.
1.4.2.6 Reaction cuvette rinsing mechanism

! Warning:
△
● Please don’t touch the rinsing mechanism when operate, or it may cause body injury or instrument damage
● Avoid directly contact with body, or it may cause infection. Please adopt protective measure. In case of skin
contact, flush the area with water, rinse immediately with plenty of water and seek medical advice.
(1) Function
Eliminates the reaction solution, rinse the reaction cuvette,injects and eliminates purified water which
used for test cell blank
(2) Rinsing composition of nozzle
Nozzle1 Nozzle 2 Nozzle 3 Nozzle 4

C D A G A B E B
Reaction Disk
Figure 1-8 Rinsing nozzles arrange
The cleaning of one reaction cuvette needs five steps：

Move away the waste and add detergent.
Step 1：Nozzle 1D suck reaction mixture，1C distribute detergent into colorimetric tube.
Step 2：Nozzle 2G suck the detergent in the colorimetric tube，and then 2A distribute deionized water into
colorimetric tube.
Step 3：
Nozzle 3B suck deionized water in the colorimetric tube，and then 3A distribute deionized water into
colorimetric tube
Step 4：photometry，the colorimetric tube which is full of deionized water can conduct cuvette blank
absorbance measurement
Step 5：nozzle 4B suck deionized water in the colorimetric tube，meanwhile, wipe the colorimetric tube.
The distribution of 4 nozzle
A distribute pure water used for rinsing… …………………… ………1
B suck water used for cleaning………………… ………………………1
C distribute detergent……………………………………………………1
D suck reaction solution…………………………………………………1
E distribute pure water……………………………………………………1
F suck pure water…………………………………………………………2
G suck detergent…………………………………………………………1
（3） Operation
Power on：First descend by about 5mm and then rise .
Analysis ：According to the direction of figure1-8―Rinsing nozzles arrange‖ to conduct reaction cuvette
cleaning and bottle blank measurement.
（4） Operation check
Single-click the ―maintenance‖ key, select ―mechanism operation check‖, and input the check times. Click
―Execute‖. If abnormality exists, instrument will issue alarm.
（5）Mounting/ Dismounting
Unscrew the screw counter-clockwise, lift the cleaning unit; in installation, matching the cleaning unit with
seat pin, and tighten the screws.
1.4.2.7 Reagent cooling system
(1) Composition and function:

Reagent cooling
(2) Specification
Temperature: 5℃～15℃ or 2℃～8℃
Warning:
● Even the analyzing system is power off, cooling system is still at working status. The cooling system
only stop working when main power supply is cut off.
● The usage and storage of reagent should be performed strictly according to user manual.
1.4.2.8 Optical system
(1) Function
When the reaction disk rotates, the absorbance of purified water or reaction solution is measured in each
reaction cuvette. As figure1-9 shows.
(2) Specifications
Carry out photometry with dual-wavelength or single-wavelength at wavelengths: 340 nm,380 nm,405
nm,450 nm,480 nm,505 nm,546 nm,570 nm,600 nm,660 nm,700 nm,750nm.
Wavelength accuracy: ±2nm
Measuring range: 0 -3.3 Abs
Spectral bandwidth: FHW 8 to 10nm
Detector: Silicon photodiode
Light source: 12V, 20W halogen lamp
Figure 1-9 Photometer

1.5 Instrument Symbol
Symbol Meaning
The prompts to pay attention, otherwise, may result in personal

injury.
To perform as the instruction under the symbol, emphasize the

important information and special contents.
To perform as the instruction under the mark, or it may cause

biological infection
AC symbol
Only diagnostic use
Storage at
Batch code
Use by
Serial number
Measurement Control
Date of Manufacture
Manufacture by
Grounding terminal
Figure 1-1
Chapter 2 Measuring Principle
The measuring principle is composed of mechanism movement principle and analyzing assay.
2.1 Mechanism movement principle

CS-T240 Auto-Chemistry Analyzer consists primarily of the sample reagent disk, sampling mechanism,
reaction disk, reaction bath, rinsing mechanism,stirring system and photometer. Operation of each
mechanism is explained according to figure 2-1:
120 reaction cuvette

position No.
Reaction cuvette
rinsing unit
Reaction disk
Reagent resetting
sample
adding
position
Stirring position Photoelectric detection

position
Reference position
No.
Figure 2-1
2.1.1 Operation flow
2.1.1.1Rinsing unit
Rinse from the first reaction cuvette (Rinse each position twice), the reaction disk will pause after rotating
38 reaction cuvette position, and then pause after two reaction cuvette position, and then pause again after two
reaction cuvette position, then stop after 80 reaction cuvette position. In the cleaning process, due to reaction
cuvette go through metering section, so the cell blank can be tested. The cell blank value can be used as the
benchmark value of absorbance (Abs). The detergent in the reaction cuvette will be sucked by detergent nozzle .
2.1.1.2 Sample and reagent adding unit
Sample and reagent adding share one disk ,one set of injector, the order is reagent and then sample. When
the reagent control panel get adding sample command, the sample reagent disk will move to the corresponding
reagent position, the probe will move to the up direction of the reagent. And the then, the probe conduct rinsing,
the sample reagent disk will move to the corresponding sample position, the probe move to above the sample. At
this point, the probe will wait above the sample cup. When the reaction disk pause after two reaction cuvette
position, the probe will move to the reaction cuvette position to add sample, and the move to the rinsing position.
In addition, in reagent 1adding, reagent and sample is added at the same time. In reagent 2 adding, only
reagent is added.
Probe unit will move to the reagent or sample position upon receipt of an sample adding command, the
probe will move to the top of reagent or sample. With the liquid level sensor, the probe tip will stop after enter
into the sample. And then the probe will move to above the reaction cuvette, to discharge reagent or sample.
Then the probe will conduct rinsing.
2.1.1.3 Stirring unit

Start testing，reaction disk rotate 5 circles＋42 reaction cuvettes（about 100s）thatis（reaction disk move 38
＋2＋2 circles），mixer begin to work. The one circle process of the reaction disk is 38 cuvettes（pause）＋ 2
cuvettes（pause）＋2 cuvettes（pause）＋ 80 cuvettes（pause），the time is 18s.
2.1.1.4 Reaction disk unit

① Reference position of parts:
Reaction disk reset point is in the 71position；
Reagent 1 ,Reagent 2 and sample proble in position 1 and the mixer probe in position 3
Reaction cuvette rinsing probe is in position 71、73、81
② Reset Process：
The probes move to the last point → sample reagent probe、the mixer move to the rinsing bath position →
the reaction cuvette move to 0（cuvette 1is in the position of）
；
③ Reacton disk：
From the reset point, the reaction disk roatate counter clock is the distance of 38、2、2、80 cuvette. The
sample, reagent adding, mixture and rinsing will be carried out during the pauses.
④ Reaction cuvette rinsing:
Reaction cuvette rinsing is carried out at the beginning of testing. Therefore, reaction diks is rinsed once in
each circle. The time is fixed—18s
Rinsing sequence：
Odd NO.：1 → 3 → 5 → 7 → 9 → …… →117 → 119 （18 minutes，60 times）
Even No.：2 → 4 → 6 → 8 → 10 → …… → 118 → 120 （18 minutes，60 times）
Figure 2-2、2-3：
38 、 2 、 2 、 80
poisition ， reaction
cuvette 3 begin to
rinse
Original position
Reaction cuvette 1
begin to rinse
Figure 2-2 Figure 2-3
⑤ Sampling：
Sampling is conducted after10 times’ rinsing. After position resetting, reaction cuvette 1 is at the position of
71，after 10 times’ rinsing, reaction cuvette 1is at the position of sampling position. As show in figure 2-5
No. 1 sample and

reagent adding
position
Figture 2-4
Therefore, the sequence of sampling is the same as reaction cuvette sequence
Odd No.：1 → 3 → 5 → 7 → 9 → …… →117 → 119
Even No.：2 → 4 → 6 → 8 → 10 → …… → 118 → 120
The first 10 times rinsing need 5×18s＝90s，and then sampling after 18s. The sampling time is after 2
positions. In the process of continuous sampling，the sample reagent probe need to finish sucking, sampling,
rinsing in 18 seconds. The individual dilution steps should be added. When the ISE functioned added, a sampling
process of sucking and ISE position is needed.
21
⑥ Reagent adding and mixture：

The reagent and sample share one probe and one mixer.
Reagent 1、2 adding and mixe have fixed position and time. Single reagent can be used as reagent 1.
Reagent 1adding and mix：
Reagent 1sampling is after the beginning of the test. Reaction disk rotate 5 circles＋38＋2 cuvettes to
sample。
The mix of reagent 1 is after reagent 1 adding.
No.3 mix position
Figure 2-5
Therefore，reagent 1sampling and mix is donein one rotate(18s).
Reagent 2 sampling and mix：
Reatgent 2 sampling is after the beginning of test. Reaction disk begin to sample at the 25 th circle, at 38＋
2＋2＋80 reaction cuvette.The mix of reagent 2is after reagent 2 adding.
2.1.2 Metering Characteristics

The instrument adopts the whole reaction monitoring system, which continuously measures the absorbance of
reaction solution for a reaction time of 18 minutes. The reaction disk rotates 1 turn plus 2 patches in about 18
seconds and during this time the absorbance is measured for all of 120 reaction cuvettes which go across the optical
axis of the photometer. For each reaction cuvette, measurement is made 10 times (10 photometric points) in a
reaction time of about 3 minutes. 20 times (10 photometric points) measurement are made during 6 minutes.
30 times (30 photometric points) measurement is made during 9 minutes, 49 times (49 photometric
points)measurement is made during 15 minutes. The lens condenses the white light emitted from the light source
lamp, which passes through reaction cuvette and is to be separated by concave grating. The separated respective
wavelength components are simultaneously received on the 12 fixed detectors and amplified by 12 amplifiers, then
logarithmically converted to obtain the absorbance or absorbance change rate. In 2 wavelengths
photometry, concentration is measured by the value of the difference of dominant wavelength and
complementary wavelength. This means that the photometer features a correcting effect for lipemia, hemolysis and
icterus of sample and has a compensating effect for fluctuation in source voltage, thus realizing stable measurement.
2.2 Analytical mode
The assay mode of Auto-Chemistry Analyzer is based on the Beer-Lambert law that the material selective
absorption light.
The main principle is: When monochromatic light with specific wavelength passes through the cuvette with
sample, the monochromatic light absorbency and sample liquid concentration are varies directly as the distance
which is passed through sample liquid by light:
1 I
A = lg（）= lg（ 0 ）= ε b c
T It
A －Absorbency of the light when passing through liquid .

T －Transmitted intensity and incident intensity ratio: transmittance It/I0.
I0 － Incident intensity .
It － Transmitted intensity.
ε － Molar absorption coefficient of solution（ml×mmol 1×cm 1）.
－－
c － Mol concentration of the solution（mmol/ml）.

b － Solution layer thickness（cm）.
Solution layer thickness (b): Optical path, which is fixed by instrument. Molar absorption coefficient (ε) is the
correlation coefficient of the wavelength, solution and solution temperature. Linear relationship is displayed
between solution thickness and absorbency when in stable temperature and single wavelength（ε value is given
on the reagent bottle by factory）
If the sample liquid adequate distribution, interaction between liquid and incidence monochromatic light only
happens during absorbing process. No fluorescence, disperse and photochemical appear. No interaction
between substances in the solution while absorbing process. The absorbency possess conducts nature, and this
condition conforms to the Beer-Lambert law.
2.2.1Assay mode variety
As to how to set the assay parameter and standard liquid parameter, please refer to user manual. Assay mode is
shown as table 2-1:
Method Item Photometry point Cell blank Formula Note
1-point L–0–0–0 B1 + B 2 + B 3 Al + Al−1

Assay 1 ≤ L ≤ 49
3 2
t :time(minute)b
2-point L– m–0–0 B1 + B 2 + B 3 ( AM + AM −1 ) − k( AL + AL −1 ) etween
Assay 1≤ L＜m ≤ 49 photometry point
3 2
L,m
AM + AM −1 AL + AL −1
2-point L– m–0-0 B1 + B 2 + B 3 −
rate assay 1≤ L＜M ≤ 49 2 2
3
t
L– m–0-0
Rate A B1 + B 2 + B 3 △A（M-L）
1 ≤ L＜m ≤ 49
Assay
L +2＜m 3
Table 2-1 Assay mode table
23
Explanation of symbols:
L,m, : Photometric points

B1、B 2、B3 :Stopped cell blank
B1,B2,B3 :Passed cell blanks
(B1,B2,B3)/3 : Mean value of 3 times passed cell blanks
Ax :Absorbance at photometric point x
△A(m-L) :Change in absorbance per minute between photometric points L and M
k :Liquid volume correction factor
a
S + ∑ Rj
j =1
k= b
S + ∑ Ri
i =1
S :Sample volume
Rj,Ri a: No. of reagents without correction
b: No. of reagents with correction
Note 1: The 21 th Photometric point won’t be stirred after adding reagent 2. Stirred when the reaction disk
pauses after rotates one circle plus 2 pitches plus 80 more pitches.
Note 2: liquid in the reaction cuvette should be more than or equal to 150 ul, less than or equal to 550ul.
Note 3: Do input 0 if the photometric point is not used.
(1) 1-point Assay
Endpoint assay in which absorbance is measured at a designated photometric point (specific time point when
reaction reach balance) after addition of sample and reagent. Figure 2-6 explains the 1-point assay.
Figure 2-6 1-point Assay

(a) Photometric point : 【L】-【0】-【0】-【0】（1< L ≤ 49）
(b) Calculation of absorbance
The average of absorbance at measurement points L and L-1 is used.
AL + AL−1
AX =
2
(c) Calculation of concentration

C X = {K × ( AX − B ) + C1 }× IFA + IFB
B1~B2: Passed cup blank
R1, R2: Reagent adding position
Cx concentration of standby sample
C1: Concentration of standard 1 solution(reagent blank)
K: Factor
B: Absorbance of blank
IFA and IFB: Instrument constants, representing slope and intercept
(d) Analytical idems TP, ALB, etc.
(2) 2-point Assay
Endpoint assay in which measurement is made twice at different points to obtain the difference in
absorbance. One point is measured as the action initial, the other point is measured when the action reach
endpoint or balance. The difference between the absorbance of two photometric points is used for
calculation sample concentration. Figure 2-7 explains the 2-point assay:
Figure 2-7 2-point Assay

(a) Photometric point : 【L】-【M】-【0】-【0】（1≤ L ≤ 49）
The difference between the average of absorbance at measurement point m and m-1 and that at measurement
points l and l-1 is used.
( AM + AM −1 ) − k ( AL + AL −1 )
AX= 2
a
S + ∑R j
j =1
k= b
S + ∑ Ri
i =1
a: No. of reagents at AL measurement

b: No. of reagents at Am measuremen

C X = {K × ( AX − B ) + C1 }× IFA + IFB
25
SB: Stopped cup blank B1~B3:

Passed cup blank
R1~R2: Reagent adding position
Ax: the defference between the photometric point M and L
Cx concentration of standby sample
C1: Concentration of standard 1 solution(reagent blank)
K: Factor
IFA and IFB: Instrument constants, representing slope and intercept
(d) Analytical idems CRE, etc.
（3） 2-point Rate Assay
Measurement is made twice at different measurement points (The two point are neither measured initial nor
endpoint) to determine the change in absorbance per minute in order to calculate sample concentration. For
check of reaction limit level, refer to Figure 2-8:
Figure 2-4 2-point Rate Assay

(a) Photometric point : 【L】-【M】-【0】-【0】（1< L <M≤ 49）
The difference between the average of absorbance at measurement points M and M-1 and that at
measurement points L and L-1, then divide the result by time.
( Am + Am−1 ) ( AL + AL−1 )
−
2 2
AX= t
t : Time (minute) between measurement points L and M
C X = {K × ( AX − B ) + C1 }× IFA + IFB

R2-R2: Reagent adding position
Ax: change in absorbance per minute between measurement points L and M
Cx: Concentration of standby sample
C1: Concentration of standard solution 1(reagent blank)
K: Factor
IFA and IFB: Instrument constants, representing slope and intercept.
(d) Analytical idems BUN, CRE etc.
(4) Rate A Assay
Ordinary Rate Assay. The concentration or activity level is obtained from the change in absorbance between
the specified measurement points. Figure 2-9 explains the Rate A Assay.
Figure 2-9 Rate A Assay

(a) Photometric point : 【L】-【M】-【0】-【0】（1<L <M≤ 49） L+2<m)
The change in absorbance per minute between measurement point L and M is obtained by the least squares
method
AX=△A(M-L)
C X = {K × ( AX− B ) + C1}× IFA + IFB
R2-R2: Reagent adding position
△A(M-L): change in absorbance per minute between measurement points L and M
C1: Concentration of calibrator 1(reagent blank)
K: Factor
B: change in absorbance per minute of calibrator 1
(d) Analytical idems AST, ALT, etc.
2.2.2 Calibration Method
（1）Linearity Method (K-factor assay)
The absorbance and input K value of blank (or calibrator 1) is measured to prepare a working curve. Figure
2-10 explains the linear method.
Figure 2-10 1-point linearity

(a) Calibration Parameter input
Calibration type: 【1-point linear】
Calibration point: 【1】 (number of calibrator sample )
Span point: 【0】
(b)check K factor
Input K factor in the ―calibration result ‖
(c) Calculation of parameters for working curve
S1ABS (B): Change in absorbance per minute of blank (standard 1)
K: Input value.
C1: Concentration of standard 1（reagent blank ), input value.
(d) Calculation of concentration
C X = {K × ( AX − B) + C1}× IFA + IFB
AX: Calculated absorbance or change of absorbance per minute.
(e) Applicable assay
1-point assay, 2-point rate assay, 2-point assay, rate A assay
(2) 2-point linearity

Blank (or calibrator 1) and calibrator 2 are measured to prepare a linear working curve Figure 2-11 explains
the linear method.
Figure 2-11 2-point linearity
(a) Calibration Parameter input Calibration type:

【2-point lineariaty】 Calibration point: 【2】
(number of calibrator ) Span point: 【2~6】
(b) Calculation of parameters for working curve
S1ABS (B): Absorbance or change in absorbance per minute of blank (standard 1)
K: Calculated from measured values and input values of blank (standard 1) and standard sample (standard 2)
C1: Concentration of standard 1（reagent blank)
C2: Concentration of standard 2
A2: Absorbance or change in absorbance per minute of standard 2.
C 2 − C1
K=
A2 − B
C X = {K × ( A− B ) + C1 }× IFA + IFB
AX: Absorbance or change in absorbance per minute
(d) Applicable assay
(3) Multi-point linearity
Blank (or standard 1) and standard samples (standard 2 and standards 6) are measured and linear working
curve. Figure 2-12 explains the linear method.
Absorbance
Concentration
Figure 2-12 Multi-point linearity

Calibration type:【 multi-point linearity】
Calibration point: 【3-6 】(number of standard sample)
Span point: 【3-6】
S1ABS (B):Linear primary regression intercept for absorbance or change in absorbance per minute of blank
(standard)
K: Inverse number of working curve slope in the result of linear primary regression.
S1ABS and K values can be calculated by the formulas below:
X × Cr
S1ABS (B) = A −
Y
A1,A2: Each measured absorbance in duplicate measurement of standard(1)

n : No. of standards(N) ×2
Cri: Concentration of standard (i)
C X = {K × ( AX − B) + C1}× IFA + IFB
AX: Absorbance of sample or its change per minute.
(d) Applicable assay
(4) Logit-log 3P (Non-linearity Method)
This is applied to a working curve in which the absorbance converges as the concentration increases. Figure
2-13 explains the non-linearity method.
Figure 2-13 Logit-log3P

Calibration type: 【Logit-log3P】
Span point: 【0】span calibration invalid
B: the absorbance or approximate value measure of the absorbance change per minute when CX
approaches ∞.
K: blank (standard 1) absorbance or value calculate by the approximation formula of the absorbance change
per minute subtraction B
a : Constants in approximation formula. Automatically calculated.
S1ABS,K,a are displayed on the Calibration List screen.
(c) Calculation of concentration.
C X = (C + C1 ) × IFA + IFB
K
AX = B +
1 + aC)
1 ⎧ K − ( A X − B) ⎫
C= ×⎨ ⎬
a ⎩ AX − B ⎭
C1: Blank concentration.
K: Constants in approximation formula. The more Cx approaches ∞, AX approaches B
When K＜0, AX≤B+K or K＞0,When AX≥B+K,C=C1
IFA,IFB Instrument constants, representing slope and intercept.
(d) Calculation of SD value
∑∑ (A )
N 2
, 2
IJ − A1
i =1 j =1
SD =
2N − 3
（N=3～6,j=1or 2）
（Aij-Ai’）: Difference between approximate absorbance Ai’ and measured value Aij or A12. Each standard
sample is measured in duplicate so the number as measurement points Aij is 12 at maximum
1-point assay, 2-point rate assay, 2-point assay, Rate A assay.
(5) Logit-log4P (Non-linearity method 2)
It is applied to a working curve in which the absorbance converges as the concentration increases. Figure
2-104explains the non-linearity method.

(a) ―Calibration Parameter‖ input

Calibration type: 【Logit-log4P】
Calibration point: 【4-6】 (number of standard sample)
Span point: 【0】
B: approximation for the absorbance or it’s change per minute when CX approaches ∞.
K: blank (standard 1) absorbance or value calculate by the approximation of the absorbance change per
minute subtraction B
a ,b : Constants in approximation formula. Automatically calculated.
S1ABS,K,a are displayed on the Calibration List screen.
C X = (C + C1 ) × IFA + IFB
K
AX = B +
1 + aC b )
1 ⎧ K − ( AX − B) ⎫
C = b× ×⎨ ⎬
a ⎩ AX − B ⎭
When K＜0, AX≤B+K or when K＞0,AX≥B+K C1=0
∑∑ (A )
N 2
, 2
IJ − A1
i =1 j =1
SD =
2N − 4
（N=4~6,j=1or 2）
(6) Logit-log5P (Non-linear method 3)
There is no distinct difference between the working curves prepared by the non-linear method 2 and 3.
However, in some cases, the non-linear method 3 allows more accurate approximation this method has one
more calculation parameter than the non-linear method 2. Figure 2-15 explains the non-linear method 3.
Absorbance
Concentration

Calibration type : 【Logit-log5P】
Calibration point: 【5-6】( number of standard sample )
Span point: 【0】Span point calibration invalid.
B: approximation for the absorbance or it’s change per minute when CX approaches ∞.
K,a,b,c: Constants in approximation formula. Automatically calculated.
S1ABS,K,a ,b,c :are displayed as S1ABS,K,A,B,C on the Calibration List screen.
AX − B
a+b·lnC+c·C-ln{ }=0
K − ( A − BX )
Calculate C according to the Newton approximation formula.
C X = (C + C1 ) × IFA + IFB
K
1 + exp× (− a − b × ln C − c × C )
AX=B+
When K＜0, AX≤B or when K＞0,AX≥B,C=0
∑∑ (A )
N 2
, 2
IJ − A1
i =1 j =1
SD =
2N − 4
（N=5~6,j=1 or 2）
sample is measured in duplicate so the number as measurement points Au is 12 at maximum
(7) Exponential function method (Non-linear method)
Unlike non-linear methods 1,2 and 3.Exponetial function method prepares a working curve in which the
absorbance disperses as the concentration increases. Figure 2-16 explains the exponential function method.
Absorbance
Concentration
Figure 2-16 Exponential function method

Calibration type: 【exponential function】
B: approximation formula for the absorbance or it’s change per minute of blank (standard 1)
K,a,b,c: Constants in approximation formula. Automatically calculated.
S1ABS,K,a ,b,c :are displayed as S1ABS,K,A,B,C on the Calibration List screen.
AX=B+K × exp{a × (ln C ) + b ×(ln C )

2
+ c × (ln C )
3
}
⎛ A − B⎞
a × (ln C ) + b × (ln C )2 + c × (ln C )3 -ln ⎜ X ⎟= 0
⎝ K ⎠
C X = (C + C1 ) × IFA + IFB
C1, C2~CN: Blank and standard concentration.
When K>0, AX≤B or when K<0,AX≥B,C=0

∑∑ (A )
N 2
, 2
IJ − A1
i =1 j =1
SD =
2N − 5
(N=5~6,j=1 or 2）
(Aij-Ai’）: Difference between approximate absorbance Ai’ and measured value Aij or A12. Each standard
1-point Assay, 2-point Rate assay, 2-point Assay, Rate A assay.
(8) Spline function method (Non-linear method)
In this method, line is connected in each section so as to form a curve as a whole. Since each section is
smoothed including the error in measured value, more accurate approximation is possible than the polygonal
line approximation. Figure 2-17 explains this method.
Absorbance
Concentration
Figure 2-17 Spline function method

Calibration type: 【Spline function】
Calibration point: 【5-6】(number of standard sample)
A(1),b(1),c(1),d(1): Constants in approximation formula, l=1～N
In the ―calibration‖ menu, S1ABS show as a (1) (intercept of absorbance axis ).
AX=a(I)+b(I) × (C X − C(1) + c(I)) × C ( X )

− C(1)2 + d (I) × (C X - C(1) )3
f × (C X − C(I )) = a × (I ) + b × (I ) × (CX − C(I )) + d × (I ) × (CX − C(I ))2 + d(I ) × (C X − C(I )) 3 − AX

C X = (C + C1 ) × IFA + IFB
C1~CN: Blank and standard concentration.
AX, A2~AN: Absorbance of sample and standard or its change per minute.
IFA, IFB Instrument constants, representing slope and intercept.
∑∑ (A )
N 2
, 2
IJ − A1
i =1 j =1
SD =
2N − 4
（N=5～6,j=1 or 2）
1-Point Assay, 2-Point Rate Assay, 2-Point Assay, Rate A Assay.
(9) Polygon method (Non-linear method)

The range between standard samples 1 to 6（5） is subject to approximation in consideration of measured
values across them and line is connected in each section so as to form a curve as a whole. Since each section
is smoothed including the error in measured value, more accurate approximation is possible than the
polygonal line approximation. Figure 2-18 explains the polygonal line method.
Absorbance
Concentration
Figure 2-18 Polygon method

Calibration type: 【polygon】
Calibration point: 【5-6】(number of calibrator)
S1 ABS is the average nalue of two measure value (absorbance or it’s change)
C 2 − C1
K=
A2 − B
B: Absorbance or it’s change rate (1)
A2: Absorbance or it’s change rate (2)
C1:calibrator (1) concentration (input value)
C2: calibrator (2) concentration (input value)
Same as calibrator（2）～calibrator（6），calculate K2、K3、K4、K5。
C X = {K N × ( AX − AN ) + CN }× IFA + IFB
1-Point Assay, 2-Point Rate Assay, 2-Point Assay, Rate A assay.
（10）Isozyme Method
In a sample in which 2 different isozymes coexist, a reagent containing inhibitor may fail to completely
suppress the activity of either isozyme alone. In this case, isozyme activity is determined from total activity
and activity residual rate. Each working curve for total activity and isozyme activity are prepared by using 2
channels. If the activity of a specific isozyme of the coexistent two can be suppressed completely by using
monoclonal antibody, etc. this calibration method is unnecessary. As figure 2-19, 2-20 shows:
Figure 2-19 Isozyme Method
Figure2-20 Isozyme Method

(a) Calibration principle

Isozymes method uses 2 reagent position. The total activity Cf is supposed to be caculated first with a
certain reagent in the isozyme P position. Calculate the activity Cm or Cn of isozymes M or N with a reagent
which can suppress N or M substance.
The isozymes M inhibitor testing isozymes M.
Generally speaking isozymes N inhibitor cannot suppress the activity of isozymes N completely, and the
activity of isozymes M is suppressed in a degree at the meantime.
The isozymes Method uses 2 channels to test the total activity and standard cost of isozymes M,N, K value
is tested by total activity channel, both two channels are used.
M and N activity residual rate of M and N is calculated by inhibitor (ratio between absorbance of standard 3
and standard 4 in two channel.).Calculate the total activity and isozymes M activity upon above method. The
activity of isozymes N is observed by calculation.
(b) Input of parameters:
Reagent: Reagent for measurement of total activity, reagent for measurement of isozyme activity.
Standard sample: Standard sample F (containing both isozymes M and N), standard sample M (containing
isozyme M), standard sample N (containing isozyme N)
Reagent position: isozymes M and N are placed in different positions
Entry on Chemistry parameters screen.
Make entry for each of the isozyme P and Q channels as shown in below Table:
Con. and Pos.of F Activity Con.and Pos. of M isozyme

Calibrator
(Isozyme P) (Isozyme Q) (Isozyme Q)
(concentration) (position) (concentration) (position)

Calibrator（1） Blank concentration………………….... [S1] Blank concentration……..……..[S1]
Calibrator（2） Concentration value of calibrator F……..[S2] 0…………………………….…….0
Calibrator（3） 0 ………………[S3] (Isozyme M Calibrator) 0………..[S3](Isozyme M Calibrator)
Calibrator（4）
0……………….[S4] (Isozyme N Calibrator) 0………. [S4](Isozyme N Calibrator)
Figure 2-2
S1 to S4 are calibrator code numbers of calibrator 1 to calibrator 4 respectively. Enter the same calibrator
code number in both channels for each of calibrator 1,3,4. place the calibrator of isozyme M at position
Calibrator 3 and that of isozyme N at position Calibrator (4). It is unnecessary to enter the concentrations of
calibrators 3,4 of isozyme P,Q, and it is unnecessary to enter the concentrations and positions of calibrator 2
of isozyme Q. The isozyme Q item name must be specified when that of isozyme P is set, or the parameter
can not be saved and alarm occurs, and instrument stops testing. However, the item name of isozyme P is not
needed to be specified at isozyme Q side.
Note: The item name of isozyme Q should be specified while setting that of isozyme P.
(c) Calculating K value
C 2 − C1
A −B
K= 2
B: Absorbance of blank (standard 1) or its change per minute
A2: Absorbance of calibrator F (standard 2) or its change per minute
C1: Concentration of blank (calibrator 1 )
C2: Concentration of calibrator F(calibrator 2 )

(d)Calculation of activity for total activity measurement channel (isozyme P)
CF={K·（AF-B）-C1}·IFA-IFB
C3={K·（A3-B）-C1}·IFA-IFB
C4={K·（A4-B）-C1}·IFA-IFB
CF ,C3 ,C4: is sample, activity of standard 3, standard4. Ap, A3, A4: is absorbance or change of the absorbance
per minute of standard 3 , standard 4. IFA, IFB Instrument constants, representing slope and intercept.
(e) Calculation of activity for isozyme measurement channel (Q)
CM’={K·（AM’-B’）-C1}·IFA-IFB
C3’’={K·（A3’- B’）-C1}·IFA-IFB
C4’={K·（A4’- B’）-C1}·IFA-IFB
CM: Isozyme M activity of sample. AM: Isozyme absorbance of sample or its change per minute.
C3’ ,C4’:each inhibited activity of standard 3 and 4. A3’,A4’ Each inhibited absorbance of standard 3 and 4 or
its change per minute. B: Absorbance of blank or its change per minute IFA,IFB Instrument constants,
representing slope and intercept.
(f)Calculation of activity residual rate
α=
{K × ( A , -B )+ C }× IFA + IFB
3 1
{K × ( A -B) + C } × IFA + IFB

3 1
β=
{ -B )+ C } × IFA + IFB
K ×(A ， 4 1
{K × ( A -B ) + C } × IFA + IFB
4 1
when C1=0, IFA=1,IFB=0
A3, -B,
α=
A3-B
A4， -B，
β=
A4-B
(g) Calculation of isozyme M activity CM
CM’=α×CM+β×CN
CM , − α × CM
C M = C F- C N = C F-
β
β×CM=β×CF- CM’+α×CM
（α-β）×CM= CM’-β×CF
CM , − β × CF
CM =
(α − β )
(h) Applicable assay
1-Point Assay, 2-Point Rate Assay, 2-Point Assay, Rate A Assay.
Note: Suggest operator use 6 or 5 calibrators in Logit-log5P, exponential,spline non-linear calibration.
2.2.3 Calibration types
According to the number of calibration, there are four types of calibration. Blank calibration: Only reagent
blank (calibrator 1) is calibrated. Only one calibrator other than the reagent blank is calibrated. Reagent
blank and a single calibrator are calibrated. All standard solutions specified on the Chemistry Parameters
screen are calibrated. These are selectively usable so as to meet your analytical purpose. Each calibration is
explained below.
Set the calibration method in the "chemical parameters" form.

(1) Blank Calibration
Only reagent blank (calibrator 1) is calibrated. Table 2-3 lists the calculation method for each calibration type.
(a) S1ABS calculation.
Calibration type SIABS calculation

1-point linearity （A11+ A12）/2
2 point linearity （A11+ A12）/2
Multi-point linearity {（AU+ A12）/2-（AU’+ A12’）/2}+SIABS’
Logit-Log 3P {（A11+ A12）/2-（A11’+ A12’）/2}+SIABS’
Logit-Log 4P {（A11+ A12）/2-（A11’+ A12’）/2}+SIABS’
Logit-Log 5P （A11+ A12）/2
Exponential function （A11+ A12）/2
Spline function {（A11+ A12）/2- SIABS’}+a（I）
Polygon method （A11+ A12）/2
Isozyme P （A11+ A12）/2
Isozyme Q （A11+ A12）/2
Table 2-3 S1ABS Calculation

A11, A12:1st and 2nd absorbance values of calibrator (1) measured presently.
A11’, A12’:1 st and 2 nd absorbance values of calibrator (1) measured previously.
SIABS’:Previous SIABS value
a（I）:I=1~N,N representing the number of calibrator and factor of the curve（refer to 5.3.3 Logit-Log 5P
method）
Note*: Only blank reagent can be carried out in case 1 is entered for the number of calibrator (K
factor method).
(b)Applicable calibration assay
Linear (2-point), Linear (multi-point), Linear (1-point), Isozyme P, Isozyme Q, Logit-log 3P, Logit-log4P,
Logit-log 5P, Exponential, Spline
(2) Span Calibration
This is one-point calibrator assay which only calibrate reagent blank. The calibrator which corresponds to
the span point entered on the Chemistry Parameters screen is measured, this calibration method is as follow:
（a） K factor and S1ABS calculation
Calibration type K factor calculation S1ABS
Two-point Linear （C2-C1）/（A2-S1ABS） Previous value
Full-point Linear （C2-C1）/（AN-S1ABS） Previous value
Figure 2-4 S1ABS and K value calculation

C2:Concentration of calibrator (2)

CN:Concentration of Calibrator N (N represents span point)
A2:Average of measured absorbance values of calibrator (2)
AN:Average of measured absorbance values of calibrator (N)
(b) Applicable calibration assay
Linear (2-point), Linear (multi-point).
(3) 2-point calibration

Reagent blank and a single calibrator are calibrated. The calibrator and reagent blank, which correspond to
the span points entered on the Chemistry Parameters screen, are measured. Table 2-5 explains the calculation
method.
（b） K factor and S1ABS calculation
Calibration type S1ABS Calculation K factor calculation
Two-point linearity （A11+ A12）/2 （C2-C1）/（A2-S1ABS）
Full-point linearity （AU+ A12）/2 （CN-C1）/（AN-S1ABS）
Table 2-5 S1ABS, K value calculation

A11, A12:1st and 2nd absorbance values of calibrator (1) measured presently.
CN:Concentration of calibrator N (N represents span point)
A2:Average of measured absorbance values of calibrator (2)
AN:Average of measured absorbance values of calibrator (N)
A1: Average of measured absorbance values of calibrator (1)
(b) Applicable calibration assay
Linear (2-point), Linear (multi-point).
(4)Multi-point Calibration
All calibrator (including reagent blank) specified on the ―Chemistry Parameters‖ screen are calibrated. After
this calibration, Calibration result will be updated after calibration.
(a) Calculation formula
The calculation varies from the calibration methods.
(b)Applicable calibration types
Linearity (multi-point),Isozyme P, Isozyme Q, Logit-log 3P, Logit-log 4P, Exponential, Spline
2.3 Check of measure
Various checks are performed to enhance the reliability of measured results. Below are check description
2.3.1 Calibration check
Doing the calibration analysis, you can check a variety of calibration items, such as blank level
check,discreteness check, sensitivity check, K-factor check, deflection check.
(1) Blank level check
In calibration, a warning –level alarm is issued if the measured absorbance of blank is not within the input
range of standard 1 absorbance. In this case, the result of measurement and alarm (S1ABS) are printed out.
To avoid check, enter-―-3.3～3.3‖.
(2) Discrete check
A warning level alarm is issued if the difference of the two times measured absorbance value is larger than
the set value. In calibration, each Calibrator (include reagent blank: Calibrator 1) is tested twice.
To avoid the check, enter‚3.3‛.
Discrete check is performed by the below formula:
≤Absorbance Discrete Check（ABS）

(3) Sensitivity check
If the difference of standard absorbance (average between 2 times measure) from Max. concentration
standard absorbance (sensitivity) exceeds the permissible absorbance sensitivity value (Sensitivity Limit), a
warning- level alarm is issued. In this case, alarm mark is printed out together with the result of
measurement.
The working curve of the alarmed analytical item will be renewed, and the Kvalue won’t be renewed. To
avoid the check, enter-―0‖.
Check the permissible sensitivity by the below formula:
Acalibratior(N)-Acalibration (1) > sensitivity value (ABS)
(4) K factor check
If the fluctuation in factor K value between previous calibration and current calibration is 20% ,a warning
level alarm is issued. The working curve and K factor will be renew and testing can be carried out. Make
sure check the reason of alarm.
Check the K factor by the below formula:
K this − Klast
×100%
(K last + Kthis ) / 2 ≤20%
(5) Drift rate check
In calibration, a warning –level alarm is issued if the difference between the calculated absorbance and
tested absorbance has exceeded the drift rate set value. The working curve and K factor will be renew and
testing can be carried out. Make sure to check the reason of alarm. To avoid the check, enter―3.3‖.
2.3.2 Absorbance limit
As to the Rate A assay and 2 point tate assay, correct data won’t be obtained when concentration or activity
exceeds the quantitative span. Thus, set the upper limit value and lower limit value of the absorbance, print
the alarm sign. Input the calibration value on the screen. To avoid the check, enter-0 (decrease) or 3.3
(increase).
When 4 or more than 4 tested absorbance value is not accord with the set value of reaction limit absorbance,
alarm is issued as figure 2-21shows:
Reaction limit level
Time
Input photometry range
Figure 2-21
2.3.3 Linearity Abnormal Check

In the rate A assay, relation between absorbance change and time should be Linearity. Thus, check on the
linearity is a necessity.
Select ―Linearity check‖ in ―Alarm Info.‖, and input the limit check value in corresponding textbox as figure
2-22 shows:
Figure 2-22
If not selected, even if input value in textbox, linearity check is not carried out.
(1) When number of measurement points (N) more than 9 (N>9)
Linearity is checked by dividing the difference in absorbance change between the first and last 6
measurement points by the average absorbance change for all. If the value thus obtained is beyond the limit
linearity value, alarm is printed out together with the result of measurement as figure 2-23 shows:
ΔAf − ΔAb
ΔA
×100＞Limit linearity value（%）
Photometry point
Time
Figure 2-23 Linearity check N≥9

(2) When number of measurement points (N) between 4 and 8 （4≤N≤8）
Linearity is checked by dividing the difference in absorbance change between the first and last 6
measurement points by the average absorbance change for all. If the value thus obtained is beyond the limit
linearity value, alarm is issued as figure 2-24 shows:
ΔAf ，− ΔAb，
ΔA， ×100＞Limit linearity value（%）
Photometry point
Time
Figure 2-24 Linearity check（4≤N≤8）
No linearity check is needed if the following happens.
1) The No. of photometric points under absorbance of reaction limits is less than 3.(N<3)
2) ΔA/ΔA,≤0.006 or∣ΔAf-ΔAb∣≤0.006
2.3.4 Prozone check
In immunoreaction, working curve descends if antigen concentration is abnormally high beyond the suitable
range (prozone area). This is called prozone or zone phenomenon.
This instrument can check whether concentration is in the absorbance decreasing range (post zone). For
prozone check, the following 2 methods are available: antigen readdition method in 1-point assay with
prozone check and reaction rate method in 2-point assay. To Avoid check, input ―-3.3 lower limit‖ in
―checkup value‖ function box in ―analyze parameter‖ menu.
Antigen supplement method:
Take 1-point essay for example, measure reagent 1, and take it’s value as reference value. Replace reagent
with serum diluent, which contain antigen, add 20ul. Compare the prozone limit value with absorbance
difference (before add reagent 2 and after add reagent 2).
Input method:
Prozone check value (PC value): 【】
Upper /lower limit: 【】
Analytical method: 【】
Photometric point: 【Q1】【Q2】【0】【0】
1≤Q1≤Q2≤49
Aq 2 + A( q 2 − 1) Aq1 + A( q1 − 1)
PC = −k×
2 2
K=total liquid volume when test q1/total liquid volume when test q2
When 1≤Q1≤Q2≤16 or 17≤Q1≤Q2≤110, K=1.
AQ1,AQ2:absorbance of photometric point:Q1,Q2
Absorbance
Time
Figure 2-25 Antigen supplement method

Chapter 3 Instrument Installation
To make sure normal operation, install or initialize the CS-T240 only by authorized staffs from DIRUI
Company.
3.1 Installation requirement
There are two types of CS-T240 auto-chemistry analyzer, one is sold with a cabinet, the other without a
cabinet.The first one should be placed on the cabinet,the second one should be placed on smooth cabinet or
table, and then put it on the ground.But it should not be put directly on the ground. Before installation,
operator should check the space, power and environment requirement.
3.1.1 Space Requirement
To make sure the space of maintenance, please follow the instruction as below:
● Space between left (right) side of analyzer and the wall should ≥50cm
● Space between rear panel of analyzer and the wall should ≥50cm
● Space in front of analyzer should≥100cm
● Make sure there is enough space for waste device and purified water equipment.
3.1.2 Environment requirement
Operate or store the analyzer according to the following requirement:
● Working environment: 1 5 ℃ ～32 ℃
● Relative humidity: 4 0 ％～85 ％
● Atmospheric pressure: 7 6 k P a ～106kP a
● Environment should with no dust, no mechanical vibration, no noise source and power interference
● Do not put the analyzer in the vicinity of brush motor, flicker fluorescent tube and other constant on-off
electrical equipment.
● Avoid direct sunlight, do not put the analyzer in front of heat source and wind source.
● The maximum sound 1m distance around the instrument is limited at 40dB when it is working.
3.1.3 Power requirement
● Power supply: ~2 20 V/230 V, 50 Hz

● Power:650VA
● Circuit breaker: 250V, 6A
●It can not be insert into one socket with heavy load appliance such as air conditioner, refrigerator and oven etc.
△! Warning：
Note: The unqualified environment may cause test value inaccuracy , analyzer damage and it is also harmful to
human body.
3.2 Open package

3.2.1 Procedure
Check if there is a physical damage on the packing when analyzer arrived. If yes, contact DIRUI company
or local distributor. If not, open the package according to below procedure.
● Make sure that arrow on the package is up, upright the package.
● Open the accessory box and mainframe box, check if parts in box are complete, if not, please contact
DIRUI company or local distributor.
● Check the packing and appearance of the analyzer, if there is a damage, please contact DIRUI company
or local distributor.
3.2.2 Handling method

● Only push the analyzer in short and smooth distance .
● Make sure analyzer stands upright, no slope, no side lay.
● Avoid vibration while transportation, check and debug the analyzer after transportation.
3.3 Installation procedure

Only by professional staff of company. Do not disassemble the analyzer except normally system maintenance.
3.3.1. software installation
Install hardware only by professional staff of company. Do not disassemble the analyzer except normally
system maintenance.
Install software only by professional staff of our company. User is not allowed to uninstall software unless
abnormality occurs. Uninstall the software according to the following procedure:
Put CS-T240 applied software into CD-Rom, click ―set up.exe ‖ file, installation program initialize as figure
3-1 and 3-2 show:
Figure 3-1
Figure 3-2
In Figure 3-2, click "Next" to pop up the selection form of software installation folder, and the software default
installation directory (full installation) is "C: \ Program Files \ DIRUI\CS-T240 automatic biochemical analyzer
application software \ ", as figure 3-3 shows below:
Figure 3-3
In figure 3-3，click ―change(C)…‖function key，change the install catalog of the software，and the
click―next‖function key，installation of the starting software，as shown in figure 3-4：
Figure 3-4
Click ―install‖ button in figure 3-4, initialize the software as figure 3-5, 3-6 shows:
Figure 3-5
Figure 3-6
Click ―finish‖ button in figure 3-6 to complete the installation process.After installation, the menu ―CS-T240
auto-chemistry analyzer software‖ can be shown on the screen .
3.3.2 Peripherial device connection
3.3.2.1 Connection of pure water inlet pipeline
Connect outlet of pure wate machine and pure water inlet of the analyzer.( ③ in figure1-2)
3.3.2.2 Connection of waste liquid outlet pipeline
Connect one end of the concentrated waste pipeline taken with the analyzer with the concentrated waste liquid
outlet interface ④ in figure 1-2, and place the other end into the waste liquid collector.
3.3.2.3 Connection of computer

Connect one end of the communication cable taken with the analyzer with the interface of ―RS-232‖
(figure1-2 ⑥) in the analyzer, and connect the other end with serial port of computer mainframe.
3.3.2.4 Printer installation

Make sure do the following checkup before print:
(1) Check if install the driver of printer.
(2) Check the specifications of all printing paper
3.3.3 System login
After installation, following power supply need to be got through:
General power supply（Figure1-3 ③） of the instrument, analytical system(Figure1-3⑤)and printer.
Then, click the icon on computer screen or click ―start-up‖, then find the software CS-T240 in
―program‖ and click it, after that, enter ―system logging‖ window as the figure3-7 and 3-8 show.
Figure 3-7
Figure 3-8
Input user name, password, click ―login‖ or ―enter‖ to get into the main menu of software, as figure 3-9 shows:
( Initial user name: 001, password: 001)
Note：If the inputted user name or password is wrong, wrong login will be displayed on the screen. If the
inputted user name or password is incorrect for continuous three times , the system will exit
automatically.
Figure 3-9
After successfully login, the software show as offline state, browse menu, check alarm information, user
logout function can be used at this state.
a) Connection: in figure 3-9 interface, click ― ”, connecting will show, after success of access, the
status bar will display ―standby……‖. At this time, all operation and tests can be carried out.
After logging, the instrument partially connected or not connected the cable, click the ― ‖, the
screen will show as figure 3-10 shows.
Figure 3-10
If this shows on the screen, connect after get the power supply connected.
b) Exit system:
In the window as figure 3-9 shows, click ― ‖, enter exit confirrm form figure 3-11 shows:
Figure 3-11
Click ―ok‖ in figure 3-11 to exit software.
Only exit system at off-line state. If analyzer is on-line, click ―off-line‖ button to exit system.
c)Logout user：under figure 3-9 interface，click shortcut key―Logout user‖ the screen will display as figure
3-8，the user can switch.
Note:
● In order to prevent data from being damaged or revised by other people, exiting software when doctor takes
a rest is strongly suggested. Periodically backup database in order to avoid data lose.
●. Input initial user name and password when first login, select ―user information‖ in ―management‖ menu, set
user name, password and access authority for next time login.
The analyzer will be in sleep status after 20 mins of power on.(waiting for the stability of power and
temperature).
3.3.4 Uninstallation of software
Method 1:If the uninstallation of applied software of auto-chemistry analyzer of CS-T240 is needed, please
enter ―addition or cancel program‖ in setting board, click ―delete‖ button, window ―addition or cancel
program‖ will pop up. Then window like figure 3-12 will show:
Figure 3-12
In the figure 3-12, click ―Yes‖ to complete the uninstallation.
Method 2: Click ―start‖ form, find ―Di Rui CS-T240 Auto-chemistry Analyzer‖, click‖uninstall CS-T240
Auto-chemistry Analyzer‖ can also uninstall that software. As show in figure 3-13:
Figure 3-13
In the figure 3-13, click ―Yes‖ to complete the uninstallation.

Chapter 4 Accessory Device

4.1 Barcode reader
4.1.1 Scan range of barcode reader
Barcode reader is used for identify routine reagent barcode of outer circle of sample reagent diskand the
routine sample of 25th to 44th position(It is changed as the reagent position setting changing) and 45th
position(It should be CS-anti-bacterial phosphorus-free detergent).
4.1.2 Sample container requirement
Specification:
cuvette: Φ10mm×75mm,Φ10mm×100mm,Φ13mm×75mm,Φ13mm×100mm(±1 mm)
standard cuvette: Φ14mm×37mm(±1 mm)
Orifice of the cuvette should be regular. Deformation and extrusion is not allowed.
4.1.3 Barcode using requirement
(a) Type: code 128,code 39,code 93,12of5,UPC/EAN
(b) Size: The width of barcode should be 8~12mm, and the valid length of barcode should not be more than
40mm. Blankness between start and finish line should be within 3mm when cutting barcode as figure 4-1
show:
Figure 4-1
（c） Digit of different barcode types,shown as table 4-1:
Sample barcode type Digit

Code39 5～10
Code93 4～12
Code128 5～22
12of5 4～15
UPC-A 11
UPC-E 6
EAN-8 7
Figure 4-1
4.1.4 Stick requirement of sample barcode
(a) No cockle, no contamination one the label, no line deformity when stick barcode , otherwise the barcode
reader cannot correctly read the barcode.
(b) Stick barcode on correct place:
In order to obtain correct barcode, 15mm-20mm between cuvette bottom and barcode lower line is
required. Make sure barcode is on the outer side of sample disk when place on the test tube rack., as figure
4-2 show:
Figure 4-2
Note: The ―‖, ‘’,（）are not allowed for the barcode, or it cannot be identified.
When the CODE39 is not capitalized, please add ―+‖ before the corresponding capitalized letters of the ID
code displayed on the screen and the report sheet printed after scanning.
4.1.5 Reagent bottle requirement：
Specifications：70ml、20ml、100ml
4.1.6 Reagent barcode using requirement
（a） Barcode type： code 128（17）
（b） Borcode label size：its width should be within 12mm～15mm，the length should be no more than
40mm（Figure 4-1）。
（c） Blankness between start and finish should be within 3mm when cutting barcode as figure 4-1 show.
4.1.7 Reagent barcode stick requirement
(a) Stick the barcode with no cockle, make sure there is no deformity on barcode line. Contamination is not
allowed on lable, or barcode cannot be read correctly.
(b) Stick the barcode on the correct place.
Blankness between bottle bottom and barcode should be within 15mm-25mm, thus barcode could be
read correctly.
4.1.8 The rule of reagent barcode
Barcode can be made by user according to their needs with the rule listed in the table 4-2:
Barcode Detailed explanation

Information Barcode range of barcode Remark
digit implication
Biochemical reagent
0~94 item code ( represent
different item name)
1~2 Item name CS-antibacterial
98 phosphor-free
detergent
99 CS-alkaline detergent
1 20ml
2 70ml
3 Bottle 3 100ml
specification
4 500ml
5 2000ml
1 R1
2 R2
4 Reagent type Reagent type reagent
should be 5 if item code
5 No
is ISE reagent or CS
series detergent.
0~9 Year
5~9 Production
01~12 Month
date (Lot)
01~31 Day
1 2 weeks
2 1 month
3 3 months
4 6 months
10 Expiry date 5 12 months
6 18 months
7 2 year
8 3 year
9 5 year
11~14 Bottle code 0001~9999 The XXXXXth bottle
Numeric or alphanumeric
15~17 Parity bit
(automatically generated)
Figure 4-2
Reagent barcode information can only be read by barcode reader, the information will coupling with
chemistry parameter which stored in instrument, this process is called reagent registry information. Reagent
information registration could check reagent position on reagent disk.
The read information could be showed in ―reagent information‖ menu as ―disc No.‖, ―position‖, ―reagent
name‖, ―reagent type‖.
Reagent name: Chemical name of analyze item.

Reagent position: User defined, 45 position is specially used for place CS-anti-bacterial phosphor-free
detergent.
4.1.9The using of sample reagent barcode reader
When test startup, sample disk will stop turning on barcode reader position, and then barcode reader will
read barcode. If barcode is not identified correctly, the barcode reader will repeat scanning three times.
Sample supplement cannot be taken when scanning, it can be taken only after scanning. If ―scan sample
barcode‖ is setted, sample probe will stop sampling operation, sample disk will turn to barcode reader
position, start scanning. Sample disk turns to sampling position when scanning finish. Scanning information
will be showed in ―sample register‖ and ―test result‖ menu.
Barcode reader will continually identify 1-50 sample on outer track of sample disk when processing barcode
reader checkup, and the scanned information will be showed in ― maintenance‖ menu.
―??‖ means no effective barcode exist.
Note：Regular cleaning of sample-reagent disk barcode reader reading window should be conducted.
4.2 Purified water equipment

Instrument consumes 4.5L/h water at peak value. The purified water equipment should meet the following
requirement:
① water should be obtained from tap water pipe
② water conductivity should within 1us/cm
③ water supply volume should reach 20L/h or more
④ The hydraulic pressure should within 49-343 Kpa
Note: To use/maintenance the purified water equipment, please refer to user manual, or consult the
distributer or manufacturer.
Chapter 5 Software Operation

5.1 Software interface instruction
5.1.1 Main interface composition
The main menu of software is composed of status bar, main function keypad, workspace, column tips，
shortcut keypad.
(a) Status bar: Shows display status on the top of menu, real-time display instrument status, as figure 5-1
shows:
Figure 5-1
Description:
: Represent display status: stand-by, testing, emergence stop, sampling stop, maintenance
operation, sleeping mode.
: Communication monitor mark. When communication is under normal status, the icon turns blue,
when communication abnormal, the icon turns black. Once the icon color turns from blue to black, that
indicate communication failure.
: Alarm icon. This icon occurs in status bar when alarm is issued. Click the icon, start alarm checkup,
solve the problem according to the remedy.
: Display temperature of circulating water in incubation bath, regular water temperature is

within 37℃±0.1℃. Alarm issued when temperature above 45℃. Alarm also occurs during test status. In
testing, alarm will be issued if the temperature of reaction bath exceeds（37±0.5）℃.
: Display ID information of current user. To setup, change or remove the

information, click ―user information‖ in ―management‖ menu.
: Display computer system time

(b) Main function keypad: Select menu by single mouse click. Single click on corresponding function key,
the border will change color correspondingly. As figure 5-2 show:
Figure 5-2
(c) Working space: According to the function selected by the user, corresponding function interface will show up,
Single click system setting in keypad area, as shown in figure 5-3
Figure 5-3
(d) Hint bar: instruct user how to use software, hint the input range, input method, operation error, as figure
5-4 show:
Figure 5-4
(e) Shortcut key space: for convenience use, as figure 5-5 shows.Some commonly used function key is set in
shortcut keypad area.
Figure 5-5
Click corresponding key, or press F2-F8 key. F1 is default as help key.

Click ―CS-T 240 Chemistyr analyzer ‖ key to display edition information of software. As figure 5-6 show.
Figure 5-6
Pop-up window as a model window, such as to turn off, click "Confirm" button.
5.1.2 Keyboard function
(a) Num Lock

This button is used for checking if number keypad is open..
(b) Caps Lock
This button is used for switch letter case.
(c) Shortcut Key
F1 Software help shortcut
F2 Start test shortcut
F3 Sampling stop/ continuous sampling shortcut
F4 Emergence stop shortcut
F5 System monitor shortcut
F6 Alarm information shortcut
F7 User log-out
F8 Exit system shortcut.
5.1.3 Software function frame
Sample test Edit sample, doctor, patient test information
Test result delete result, result query, review, preview, audit, print,
batch print, batch print, reaction curve
Reagent Info Manual registry, remove reagent info, barcode scan, reagent level
Calibration registry Calibration registry type and item

Calibration
Information
Calibration result Calibration data and reaction curve.
QC registry QC registry and parameter setup
QC control QC interval Analyze QC test data
Chart monthly Analyze QC data in one month

CS-240 Automatic Biochemistry Analyzer
Parameter Analyze, calibration, range parameter
Item combination Edit item combination information
Calculate item Edit calculation item information
Cross infect Cross contamination avoiding
System setup Report format Report Info, print sequence, print format setup
ISE setup ISE parameter setup
System setup Barcode, ISE, time awaken setup.
Manual item setup Add manual item.
LIS setup ISE communication setup.
User Info. Operator ID, name, password, access authority.
Hospital info Test delivery department and doctor.
Other Info. Sample type, patient type, clinic

System diagnose, report remark,item unit.
Management
Workload statistic
Database backup and restore
System log Login, maintenance, operation, alarm log

System Periodical maintenance and checkup
Maintenance
System help Provide help to user
Figure 5－7
5.2 Software Operation

Select function key of software by single click mouse button. Input value and character combine with keypad
(switch by shift + control, input method is depend on windows system).
5.2.1 Icon move
Icon moves as single mouse click on target input space or target item.
5.2.2 Function key selection
Select function key by single mouse click.
5.2.3Open Form
In order to open form, click function key corresponding to form. Form is divided into mode form and modeless
form.
Mode form: other menu cannot be opened until mode menu is closed.The setting of adding items of parameter
analysis, as figure 5-8 show:
Figure 5-8
Modeless form: other menu can be opened when modeless menu open like form ―check th result‖As figure 5-9
show:
Figure 5-9
5.2.4The operation of list box and scroll bar

(a) List box
List box is used for displaying part of all information. List box is also used for finding and selecting needed
information from displayed information. As figure 5-10 shows:
Figure 5-10
(b) Scroll bar

Scroll bar is used for adjusting display range in list box. Scroll bar is divided into longitudinal scroll bar and
horizontal scroll bar. As figure 5-10 shows:
Single-click ― ‖ or drag and pull the key ― ‖ to view the content.

5.2.5 Pull down menu operation
Click ― ‖ on the right side of menu to open or close pull-down menu. More information can be showed in
pull-down menu. Once select , the selected item can be showed on the top column, and pull-down menu
disappears at the same time.
5.2.6Button box and check box
Button box: only one function can be chosen among many functions, for example, ―Manual Registration
Form‖ ―Barcode‖ and ―Reagent Name ‖can not be chosen at the same time, as figure 5-11 shows:
Figure 5-11
Check box: more than two functions can be chosen at the same time, for instance ―Calibration Register‖
interface,multiple calibration items can be selected at the same time, as figure 5-12 shows:
Figure 5-12
5.3 Instrument standard specification

Performance index Standard specification
Plat image grating spectro photometry system,photometry by 12
Wavelength range wavelength. wavelength:340, 380, 405, 450, 480, 505, 546, 570, 600,
660, 700, 750nm
Wavelength
featur
Basic
±2nm
precision
e
Reaction temperature 37℃±0.1℃

Measure method End point assay, Rate assay,2-point assay
Measure speed Constant speed 200 test /hour，300tests /hour with ISE
Reagent position 、 The reagent and sample share one disk, totally 66 positons, User
sample position defined proportion of reagent position and sample position. (The
maximum reagent position is 42, the minimum is 6.)
Sample type Serum,plasma, urine, cerebrospinal fluid, ascitic fluid
Reagent+Sample System
Sample Volume 3～50ul

Sample liquid level
Integrate with sample reagent probe.
sensor
Reagent volume 10～450ul
Reagent bottle 20mL、70mL、100mL
volume
Reagent restore Temperature within 5～15℃ or 2－8℃,adopt semi conducting cooling
temperature
Reagent liquid level
Integrate with sample reagent probe.
sensor
Reaction cuvette
Discrete
type
Reaction cuvette
6mm
optical diameter
Reaction cuvette 6 unit，each unit 20，totally 120
number
Reaction liquid
Analyze
system
150～550ul
volume
Light source 20W/12V long life quartz halogen lamp
Absorbance range 0～3.3ABS
Quality control QC interval, Montly QC
Automatic rinsing Rinsing cuvette automatically.
Stirring mechanism Singleness stirring after reagent adding
port Standard RS-232
Data Printer stylus printer,support user-defined mode
system
Connecting LIS/HIS
Connect with LIS/HIS system
system
Weight About 120Kg
Integrated
Equipment size 998 mm×752 mm×517mm(length×width×height)
system Power consumption
650VA
（VA）
Table 5-1
Note： (1) It may affect the accuracy of test results when the total volume of reaction solution is150 ul.
(2) According to different test condition,sometimes instrument processing capability is lower than 200 test/hour.
Test condition Processing capability lower degree（estimated）
Test after sample pre-dilution 100 tests/hour（test under pre-diluent condition）
At least 100test/hour（reaction cuvette,sample probe）

Cross contamination avoiding function
100～200test/hour（sample reagent probe）
Table 5-2
Chapter 6 Instrument Operation
6.1Operation overview
Table 6.1 shows the operation flow. For detailed operation, please refer to 6.2.
Consult
Operation step Form / key Operation
index
1. Check before operation Check before turning on power 6.2.1
2. Connect water unit and CS-T240 Logging in Open water faucet, turn on the power of purified
Auto-chemistry Analyzer power water unit and CS-T240, input operator’s ID No.
6.2.2
Log Software and password.
3. Check instrument status Please refer to chapter 13 Alarm and Handling of
1) Check alarm Alarm information Instrument Failure
2) Check light quantity of photometer System maintenance Execute light quantity checkup, check if test value
3) Check cell blank is within regular range
6.2.3
4)Check temperature of incubation bath. System maintenance Execute cuvette blank check, check if test value is
Status bar witin regular range
Check if temperature of incubation bath is within
37.0℃±0.1℃
4. Check analytical conditions Add chemistry item
1) Item adding System setup Check chemistry parameter
2) Chemistry parameter input and System setup Check calibration curve and K factor 6.2.4
confirm Calibration info
3) Check K value
5. Reagent preparation (reagent info)1) Check reagent remaining volume and remaining
1)Check reagent residuel volume Reagent info test times. Place the reagent at the corresponding
6.2.5
2) Preparation for photometry item and position.
preparation for ISE item Reagent info
6. Setup of calibration and control item Calibration info Check item name of calibration analysis
6.2.6
Quality control Check item name of QC
7. Sample registration and test Sample registration Conducting single or batch sample registration,
single emergency sample registration, patient 6.2.7
information editing, modification and deletion.
8. Test Place sample on the sample+reagent disk
Sample Test Start test Implement "Start test" to carry out analysis
(1) sample preparation
(2) Send test instruct Place control on the sample+reagent disk
QC Test Implement "QC" ->‖QC test‖to carry out analysis
(1)Control preparation QC test 6.2.8
(2) Send test instruct
Calibration Test
Place calibrator on the sample+reagent disk
(1)Calibrator preparation Calibration test
Implement "Calibration infomation"
(2) Send test instruct
->‖calibration test‖to carry out analysis
9. Testing Process System moniter Moniter the instrument when testing.
(1)System moniter Sampling Carry out sample edit when testing and click start
(2) Sampling stop/continue stop/continue analyze. 6.2.9
(3) Emergence stop Emergence stop
(4) Sample superaddition. Sample registry
10. Check test result (result data) Result To search, amend, delete test result, check, print
6.2.10
sample reaction curve
11. Recheck sample test Test result Check the recheck item condition. In ―recheck‖
form, click ―start recheck‖, and send the recheck 6.2.11
instruction.
12 Completion of analysis Test result Check, confirm and printout the test result. 6.2.12
1) Recheck result System management Periodical backup database, one week is
2) Database backup suggested.
3) System dormancy Instrument could automatic start at specified time
4) Turn off instrument after setting
5) Preparation of next operation Cutting instruments, computers, pure water
supply device power for next time use.
Table 6-1 Overview of the operation process
6.2Detailed operation
6.2.1 Check before measurement

The following check should be carried out before testing：
（a）Check power supply and voltage.

（b）Check communication wire and power wire which connecting host computer, instrument and printer. Make
sure the wires are well connected .
（c）Check if print paper is enough, add paper if necessary.
（d）Check if there are water drops, contamination and bending of reagent probe, sample probe and stirring
mechanism.
（e）Check if the detergent is enough. Place CS anti-bacterial phosphor-free detergent at 45th position on sample
reagent disk. For detailed information please refer to ―12.1.3 detergent ‖.
（f）Check if waste solution bottle is empty. Ignore this operation if drainage device is connected with down
pipe.
（g）Check if there are air bubbles in syringes ( leakage and air bubble may cause incorrect data)
! Warning :
△
CS serial detergent is corrosive liquid. In case of skin contact, flush the area with water, rinse immediately
with plenty of water and seek medical advice.
6.2.2 Power on and software login
（a）Turn on the power of purified water, and open the water faucet.The purified water device should use one
power supply alone.
（b）Turn on the power supply of CS-T240.The main power switch lies in the left lower side of instrument.
Turn on the switch when there are reagents in sample reagent disk, therefore the cooling system may under
normal working condition. The power of analyzed part lies in the left lower side of instrument.

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CS-T240 Operation Manual

Chapter 7 Calibration Information.................................................................................................................... 105

Chapter 1 Brief Introduction

1.2 Main Technical Index

Instrument structure: Discrete system

1.3 Composition of instrument

①cover symbol ②cover ③detergent and detergent sensor ④model

1.3.2 Back picture

①Syringe ② back nameboard ③purified water injection inlet

Figure 1-3 Leftside of instrument

1.4.2.1 Sample reagent disk

1.4.2.2 Sampling mechanism

① Rinseing bath of pipetting probe ② Pipetting probe

1.4.2.3 Reaction disk

Figure 1-6 Reaction Disk

1.4.2.4 Incubation bath

1.4.2.5 Stirring mechanism

Figure 1-7 Reaction disk

1.4.2.6 Reaction cuvette rinsing mechanism

Nozzle1 Nozzle 2 Nozzle 3 Nozzle 4

The cleaning of one reaction cuvette needs five steps：

(1) Composition and function:

1.4.2.8 Optical system

Light source: 12V, 20W halogen lamp

Figure 1-9 Photometer

1.5 Instrument Symbol

The prompts to pay attention, otherwise, may result in personal

To perform as the instruction under the symbol, emphasize the

To perform as the instruction under the mark, or it may cause

Only diagnostic use

2.1 Mechanism movement principle

120 reaction cuvette

Stirring position Photoelectric detection

2.1.1.3 Stirring unit

2.1.1.4 Reaction disk unit

Figure 2-2 Figure 2-3

No. 1 sample and

⑥ Reagent adding and mixture：

No.3 mix position

2.1.2 Metering Characteristics

A －Absorbency of the light when passing through liquid .

c － Mol concentration of the solution（mmol/ml）.

Method Item Photometry point Cell blank Formula Note

1-point L–0–0–0 B1 + B 2 + B 3 Al + Al−1

Table 2-1 Assay mode table

L,m, : Photometric points

Figure 2-6 1-point Assay

(c) Calculation of concentration

Figure 2-7 2-point Assay

a: No. of reagents at AL measurement

(c) Calculation of concentration

SB: Stopped cup blank B1~B3:

（3） 2-point Rate Assay

Figure 2-4 2-point Rate Assay

B1~B3: Passed cup blank

Figure 2-9 Rate A Assay

Figure 2-10 1-point linearity

(2) 2-point linearity

Figure 2-11 2-point linearity

(a) Calibration Parameter input Calibration type:

Figure 2-12 Multi-point linearity

A1,A2: Each measured absorbance in duplicate measurement of standard(1)