Writing Assignment

Biology 212
Winter 2006

Scientific writing
One of the objectives of the laboratory experience this term is to develop your writing
skills. In science, writing is an important means of communication. A scientific paper
usually includes the following parts: a title (statement of the question or problem), an
abstract (short summary of the paper), an introduction (background and significance of
the research, including any hypotheses and predictions), a materials and methods section
(report of exactly what you did), a results section (presentation of data), a discussion
section (interpretation and discussion of results), and references (books and periodicals
cited in the introduction or discussion sections).

Assignment
Write a lab report on your experiment investigating the effect of moist and dry conditions
on optimum interplant distance that maximizes areal yield (yield per square meter of field
surface). The report should be typed with double-spaced text, 12 pt font and pages
numbered with the following components.

Title
The title should be as short as possible and as long as necessary to communicate to the
reader the question being answered in the paper.
(Additional resource http://www.wisc.edu/writing/Handbook/SciRep_Title.html)
Introduction
This section gives the background and rationale for your research question. In other
words, it places your research in context. This introduction section includes your
research question, hypothesis, and prediction. The background and rationale
information should be no more than 1-1.5 pages. You must give credit for any
information that you gain from other sources and present in your report by citing
the reference. Two references are required.
(Additional resource http://www.wisc.edu/writing/Handbook/SciRep_Intro.html)

Methods
Give a brief description of your experimental design (1 paragraph), which includes your
independent and dependent variables.

Results
This section, which is the central section of a scientific paper, has two components: (1)
text describing your results (1-2 paragraphs) and (2) a figure (graph) summarizing your
data. The figure (graph) needs to have a complete title. Cite your figure in the text
portion of your report. Do not interpret your results in this section. Review pages 14-18
in your lab manual for tips on presenting and analyzing results. Use the guidelines in the
handout attached to your in-lab assignment to prepare your graphs.

Discussion
(Additional resource http://www.wisc.edu/writing/Handbook/SciRep_Disc.html)
In this section, you will analyze and interpret the results of your experiment. Use the
following guidelines from your lab manual Appendix A: “Simply restating the results is not
interpretation. The Discussion must provide a context for understanding the significance
of the results. Explain why you observed these results and how these results contribute to
our knowledge. Your results will support or confirm your hypothesis or will negate, refute
or contradict your hypothesis but the word “prove” is not appropriate in scientific writing.
If you results do not support your hypothesis, you must still state why you think this
occurred. Support your ideas from other work (book, lectures or outside reading of
scientific literature). State your conclusions in this section.

“Complete your Introduction and Results sections before you begin writing the
Discussion. The figures and tables in the Results section will be particularly important as
you begin to think about your discussion. The graphs allow you to visualize the effects
that the independent variables had on the dependent variables in your experiment.
Studying these data will be one of the first steps in interpreting your results. As you study
the information in the introduction section and your data in the Results section, write
down relationships and integrate these relationships into a rough draft of your discussion.

“The following steps may be helpful as you begin to outline your discussion and before
you write the narrative:
 Restate your question, hypothesis, and prediction.
 Write down the specific data, including results of statistical tests.
 State whether your results did or did not confirm your prediction and support or
negate your hypothesis.
 Write down what you know about the biology involved in your experiment. How
do your results fit in with what you know? What is the significance of your
results?
 How do your results support or conflict with previous work? Include references
to this work.
 Clearly state your conclusions.
 You are now ready to write the narrative for the Discussion. Integrate all of the
above information into several simple, clear, concise paragraphs. Discuss the
results; do no simply restate the data. Refer to other work to support your ideas”.

References You must give credit to any information that you gain from other sources and
that you present in your report. At least two references are required. See resources
below for guidelines on citing references. References need to be listed in alphabetical
order by author’s last name.
No references from the Internet will be accepted for this assignment unless from an on-line
peer reviewed scientific journal.
 How to decide what information must be referenced
http://www.wisc.edu/writing/Handbook/QPA_plagiarism.html
 How to write a successful summary without plagiarizing
http://www.wisc.edu/writing/Handbook/QuotingSources.html
http://www.wisc.edu/writing/Handbook/QuotingSources.html
 How to cite references
 http://www.wisc.edu/writing/Handbook/DocCBE_NameYear_Intext.html
http://www.wisc.edu/writing/Handbook/DocCBE_NameYear_RefList.html
Additional Resources
 The Biology web site, http://osu.orst.edu/instruct/bi212/writew06.htm, has
information on resources for writing and library research.
 General information on writing a scientific report
Appendix A Scientific Writing in your lab manual.
 Evaluation form included with this assignment sheet.
 Be sure to talk with your Lab Instructor if you have any questions.

Due Date
Your Lab Instructor will assign the due date. The grade on late reports will decrease 5%
per day.

Format

Evaluation
Your lab report will be evaluated on the content (4 points) and the effectiveness of your
communication, including text and figures (5 points). See the attached page for a sample
evaluation form.
 Evaluation of Lab Report on Seedling
Biology 212
Winter 2006

CONTENT COMMENTS
Title
Introduction
Background and rationale
Research question
Hypothesis
Prediction
Methods
Description of experimental design
Procedures
Results
Table or figure (graph)
Text describing results
Discussion
COMMUNICATION
Writing
Grammar, spelling, punctuation,
capitalization
Sentence construction
Paragraph structure: sentences in
logical order supporting one main
idea in each paragraph
Organization of paragraphs
Conciseness and clarity of writing
References
Required number
Listed in Reference section
Cited in text
Figure (graph)
Each title tells a complete story
Organization of report
Headings and subheadings included
Double-space text
Pages Numbered
 Summary from Lab
Spacing Plants for Optimal Yield

The distance between plants greatly affects their areal yield (their final biomass per square
meter of field surface). If plants are too close, intense competition for light and water
causes them to grow poorly or die, and areal yield from a field of these crowded plants
will be low. If the plants are too far apart, individual plants will grow well, but there will
be so few plants per square meter that yield will be low. There is an intermediate distance
between plants at which yield per square meter is maximized. The interplant distance that
produces the greatest rate of areal photosynthesis (mg C per m2 per day), and thus the
largest areal yield, is influenced by several factors, such as temperature extremes, light
intensities and water availability.

Research Question
How would the optimum interplant distance that maximizes areal yield (yield per square
meter of field surface) change with moist and dry conditions?

Hypothesis [Write a hypothesis based on the research question on your worksheet.

Remember your hypothesis should be a biological explanation for how you think the
optimum interplant distances would change with moist and dry conditions. ]

Prediction [Write a hypothesis based on the hypothesis on your worksheet]

If the hypothesis is true, then the results of the computer model should give the following
results:
If the hypothesis is not true, then the results of the computer model should give the
following results:

Results and Discussion

You will report the results and write a discussion of your results for your Writing
Assignment. The instructions for your writing assignment will be provided in a separate
handout. To help you interpret your results for your Writing Assignment, answer the
following questions on your worksheet.

 Why do plants starve and die at very close interplant distances?

 Why do the leaf area index and soil water depletion change with increasing in
interplant distance?
 For your data what is the leaf area index at which plants are first able to realize
positive net photosynthesis?
 How does net photosynthesis for individual plants change as interplant distance
increases?
 Does individual plant photosynthesis reach a maximum value? Why?
 Which interplant distance gives the greatest net photosynthesis per square meter
for your data? Why does areal net photosynthesis decrease once this optimum
interplant distance is exceeded?

Glossary
Allocation The assignment of photosynthate to the growth of roots, stem height, stem
thickness, or leaves.
Areal photosynthesis Net photosynthesis per unit area (in Seedling, mg C/m2/day).
Fixed carbon Carbon that has been incorporated into sugar by photosynthesis.
Leaf-area index (LAI) The ratio of total leaf area to underlying soil area. When leaf-area
index exceeds one, leaves must be shading one another. High LAIs mean both severe shading
and serious water loss through transpiration.
Net photosynthesis Carbon fixed by photosynthesis minus carbon consumed by respiration.
Net photosynthesis is positive when photosynthetic production exceeds the requirements of
respiration and negative when respiration outstrips photosynthetic productivity. Net
photosynthesis must be positive for growth to occur. Negative net photosynthesis (due to
shading, water stress, or old leaves) will cause death by starvation.
Net photosynthesis per square meter (m 2) Net photosynthesis per plant multiplied by the
number of plants per square meter.
Net photosynthesis per plant A measurement equal to photosynthesis (carbon fixed into
carbon compounds) minus respiration (carbon used up by oxidation). If the result is positive,
the plant has photosynthate (carbon products produced by photosynthesis), to devote to growth
after satisfying its respiratory costs. If it is negative, the plant is starving and not growing.
Number of plants per square meter (plants/ m 2) A measurement computed from the
distance between plants.
Percent of water demand satisfied The percentage of the plant’s water needs that can be met
by the uptake of water from the soil.
Percent of the maximum net photosynthetic rate This rate would be 100% if all leaves
were new, if there was no lack of water, and if there was no shading.
Photosynthate The sugars made by photosynthesis.
Photosynthetic rate The rate of carbon fixation expressed as a percentage of the maximum
rate that would occur under optimum conditions of full sunlight, optimum temperature (25ºC),
abundant water, and all leaves new and unshaded.
Relative humidity The amount of water in the air expressed as a percentage of the maximum
amount of water that air of this temperature could hold. Low relative humidities greatly
increase water loss through transpiration.
Respiration The consumption of sugars and other organic molecules in the process of
metabolism. Every cell in a plant requires photosynthate merely to stay alive. If respiration
requires more photosynthate than photosynthesis can produce, net photosynthesis is negative
and the plant must use its food reserves
Soil water depletion The decrease of soil water (in milligrams per cubic centimeter of soil:
mg/cc) that has occurred due to the uptake of water by plants.
Stomata The microscopic openings on the underside of a leaf through which the leaf absorbs
CO2 from the air and loses water. During water stress, these openings close and
photosynthesis stops.
Transpiration Evaporation of water through the stomata of leaves.
Water demand The percentage of its water needs that a plant is currently satisfying by water
uptake from the soil. A water demand reading of less than 100% indicates water stress.