Abstracts e179
Conclusions: Asiatic acid improves TNF-a-stimulated endothelial barrier im-
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that IFN-γ induced upregulation of ICAM-1 expression, which was started at 8h,
pairment. This is associated with inhibition of cytoskeletal rearrangement and
VCAM-1 expression induced by TNF-a. Furthermore, asiatic acid also suppresses
the production of sICAM-1 and sVCAM-1, which are soluble biomarkers com-
monly used to assess endothelial function and to predict the risk of future cardio-
vascular events. These data support the use of asiatic acid as a potential therapeu-
tic agent against TNF--induced endothelial dysfunction.
LBPS 01-16 BMP4/ID2 SIGNALING PATHWAY IS A NOVEL
THERAPEUTIC TARGET FOR LATE OUTGROWTH
ENDOTHELIAL PROGENITOR CELL-MEDIATED
ENDOTHELIAL INJURY REPAIR
Wenhao Xia, Long Chen, Jianwen Liang, Chen Su, Xiaoyu Zhang, Bingbo Yu,
Yan Li, Jiang He, Jun Tao. Department of Hypertension and Vascular Diseases,
The First Affiliated Hospital, Sun Yat-Sen University, China
Objective: Endothelial progenitor cells (EPCs) play a pivotal role in endothelial
repair following arteries injury, however, the molecular mechanism of late out-
growth EPCs (LEPCs) in endothelial repair remained to be studied. Bone mor-
phogenetic protein 4 (BMP4) is involved in vascular injury-mediated mobilization
and homing of LEPCs. Here, we investigated the influence of BMP4-modified
signaling pathway in LEPC-related endothelial repair of human and underlying
molecular mechanism.
Design and Method: In vitro, after 28 days culture, human LEPCs were pre-
treated with different concentration of recombinant BMP4 or BMP4 gene transfer,
and the functional capacities and BMP4 signaling pathway of human LEPCs were
investigated respectively.
Results: Recombinant BMP4 pretreatment markedly augmented the migration
and adhesion in vitro and demonstrated a significantly accelerated in vivo en-
dothelial repair capacity of human LEPCs after transplantation into nude mice
with carotid artery denudation injury. Moreover, the main Id gene (Id2), a well-
characterized down-streaming target of BMP4, upregulated in LEPCs incubated
with recombinant BMP4. The BMP4-induced enhancement in in vitro functional
activities and in vivo endothelial repair capacity of human LEPCs were abolished
by pretreatment with BMP antagonist Noggin or shRNA-mediated knockdown
of BMP4 expression. Furthermore, BMP4 gene transfer remarkably activated
BMP4-mediated signaling pathway and facilitated therapeutic endothelial repair
capacity of LEPCs, and the improved functional activities of human LEPCs could
be inhibited by Noggin.
Conclusions: Thus, the present study demonstrates for the first time that BMP4-
related signaling pathway is essential with endothelial repair capacity of LEPCs in
human. The upregulation of BMP4-modified signaling pathway in human LEPCs
may be a novel therapeutic strategy for endothelial repair after injury.
LBPS 01-17 DISRUPTIVE MECHANISMS OF INTERFERON-
GAMMA IN ENDOTHELIAL BARRIER FUNCTION
Chin Theng Ng, Muhammad Nazrul Hakim. Department of Biomedical Science,
Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM
Serdang, Selangor, Malaysia
Objective: The endothelium maintains normal barrier integrity and blood fluidity.
The proinflammatory cytokines affect the endothelial function and endothelium-
derived factors, leading to increased endothelial permeability, impaired nitric
oxide (NO) production and altered adhesion molecule expressions. These are
associated with the progression of several cardiovascular diseases such as ath-
erosclerosis and hypertension. Interferon-gamma (IFN-γ) is a proinflammatory
cytokine primarily released by T helper cells and cytotoxic T cells. This T-cell-
derived cytokine has been shown to perpetuate the development of atherosclerosis
and promote hypertension. However, the effect of IFN-γ on endothelial function
remains to be investigated. The present study aimed to investigate the effect of
IFN-γ on endothelial permeability, NO production and adhesion molecule expres-
sions in human umbilical vein endothelial cells (HUVECs).
Design and Method: HUVECs were challenged with 10 ng/ml of IFN-γ for 4–24 h
and the permeability percentages, the NO levels and the expression of intercellu-
lar adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1)
and platelet endothelial cell adhesion molecule-1 (PECAM-1) were determined
using permeability assay kit, Griess assay and Western blot, respectively.
Results: Permeability assay showed that IFN-γ caused a biphasic increase in HU-
VECs permeability. The first phase of increased permeability occurred at 4–8 h
and the second phase of increased permeability occurred at 16–24 h. The NO
assay showed that IFN-γ significantly reduced both the basal NO levels and in-
creased NO levels donated by sodium nitroprusside. Western blot analysis revealed
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coinciding with downregulation of VCAM-1 expression. However, PECAM-1 ex-
pression remained unaltered.
Conclusions: IFN--induced endothelial dysfunction may be closely related to
the impairment of barrier integrity, reduced NO bioavailability and altered cell
phenotypes. These findings enrich the current knowledge on the mechanisms of
IFN- in disrupting the endothelial function.
LBPS 01-18 THE EXPRESSION OF GLUCAGON-LIKE PEPTIDE-1
AND DIPEPTIDYL PEPTIDASE-4 IN SHRSP.Z-LEPR
FA /IZMDMCR RATS
Sari Sugahama. Department of Pharmacy, Mukogawa Women’s University, Japan
Objective: SHRSP.Z-Leprfa /IzmDmcr rats (SPZF) were established as a model
of metabolic syndrome by crossing stroke-prone spontaneously hypertensive rats
(SHRSP) with Zucker Fatty rats. SPZF rats show hypertension, hyperphagia, obe-
sity, dyslipidemia, and insulin resistance. Many reports have suggested that gluca-
gon-like peptide-1 (GLP-1) and dipeptidyl peptidase-4 (DPP-4) affect various or-
gans. In the study, we observed the expression of GLP-1 and DPP-4 in SPZF rats.
Design and Method: Twelve-week-old male SPZF, lean phenotype (Lean), and
Zuckerfa/fa (ZF) were used in this study. After blood pressure measurement, 24-
hour urine was collected. The blood, fat tissues and kidneys were collected after
anesthesia. Blood GLP-1(7–36) amide and DPP-4 were measured. The 24-hour
urinary albumin excretion was measured. GLP-1 receptor (GLP-1R), DPP-4, and
catalase in the kidneys were detected by Real time-PCR and Western blotting.
Results: SPZF and Lean showed severe hypertension. Blood glucose, triglycer-
ides, insulin, and TBARS in SPZF were significantly higher compared with ZF
and Lean. Fat tissues in SPZF were significantly greater than in Lean and ZF. Se-
rum DPP-4 activity in SPZF was significantly higher than in the other rat groups.
GLP-1R protein expression in the SPZF kidney was not different compared with
the other rat groups. Twenty-four-hour urinary protein and albumin levels in SPZF
rats were significantly higher compared with the other rats.
Conclusions: SPZF showed higher serum DPP-4 activity than in ZF and Lean.
Elevated serum DPP-4 activity may depend on high insulin and increased fat
tissues. Elevated urinary protein and albumin excretion in SPZF was observed.
According to previous reports, elevated serum DPP-4 activity is associated with
renal injury in SPZF.
LBPS 01-19 CHANGES OF BLOOD PRESSURE AFTER
AROMATHERAPY ESSENTIAL OIL INHALATIONS:
LAVENDER AND YLANGYLANG VS. LEMON AND
ROSEMARY
Myung-Haeng Hur1, Eun-sil Min2, Ik-Lyul Bae3, Mi-Eun Kim4, Wonjong Kim4,
Joomi Ahn5, Mi kyoung Lee1, Ji-Ah Song5. 1Department of Nursing, Eulji Uni-
versity, Korea, Republic of, 2Department of Nursing, Daejeon Institute of Science
and Technology, Korea, Republic of, 3Department of Nursing, Daejeon Institute of
Science and Technology, Korea, Republic of, 4Department of Nursing, Gimcheon
University, Korea, Republic of, 5Department of Nursing, Woosong University, Ko-
rea, Republic of
Objective: The aims of this study were to find and compare the effects of aroma-
therapy essential oils (EO) inhalation in laboratory based experiment.
Design and Method: This study was the secondary analysis from two random-
ized controlled trials. The subjects of this study were the normotensive healthy
adults. The Inhaled EO in first study were Lavender and Ylangylang as a balancing
oil and parasympathetic oil in aromatherapy, and the EO in Second study were
Lemon and Rosemary as a sympathetic oils in aromatherapy. Subjects inhaled
slowly for 3 minutes from 10 cm distance from their nose.
Results: The SBP(F = 5.054, p = .008), DBP(F = 3.884, p = .024) and
sympathetic nerve activity(F = 11.322, p < .001) in Lavender and Ylangylang
experimental group were decreased after inhalation of EO. However BP in Lemon
and Rosemary group didn’t be significantly different after inhalation. But sym-
pathetic nerve activity(F = 3.781, p = .027) was significantly increased after
inhalation of Lemon and Rosemary
Conclusions: The possibility of blood pressure reduction of EO was different as a
kind of aromatherapy essential oils. In conclusion, the inhalation of aromatherapy
essential oil as a kind of parasympathetic oils will be possibly effective to decrease
the BP and sympathetic nerve activity, but the inhalation of aromatherapy EO as
a kind of sympathetic oils will be not effective to decrease the BP and will be ef-
fective to increase the sympathetic nerve activity.