Phytochemical Analysis and Amtimicrobial Activity ity Screening of the Crade Extracts from the ‘Actial parts of Tapinanthus globiferus Lina LG-Ndukwe *, .0. Amupitan end O.E. Ashonibare. Department of Chemistry, Alwmadu Bello University, Zaria, Nigeria. Abstract The aerial parts of Tapinanthus globiferus, belonging to the Loranthaceae family, which has various traditional’ medicinal applications, were studied. for Inhibtied by the extract. However, the extract did not inkibit the growth of Candida albicans, Salmonella (opi end Asperger. INTRODUCTION The plant Tapinanthus globiferus belongs to the family called Loranthaccac (Lina)'. It grows itically on trees and shrubs. Nearly all the Lorsnthaceae family grow in the tropics. The plant | was formerly named Tapinantnis bawgwensis but later renamed Tapinanthus globiferus. Plants in these genera have been reported to be of immense use in traditional medicinal applications, hence the need to investigate this particular member of the group’. For example, this plant’s family has been ei ft 43 ! In Nigeria and in the West African | region, the stem and bark infusion is used for treatment of itching skin. The re E pieces and pulverized a blender, Some quantity of the pulverised material (181.003) ‘Nigerian Journal of Chemical Research, Vol. 6, 2001Phytochemical analysis of the plant The pulverised plant santple was, techniques’ for carbohydrates, sugars, starch, cardiat glycosides, tarmins, aloes and alkaloids. ‘Tho nls baie in thie soxsening easton in Table 1. ‘Table 1, Results of the phytochemical screening on the crude sample. Positive “iiss Allaids Positive ‘Aloe Positive Carbohydrates Positive Cavizeplycorides Negative Ctyoosdes Pestve Reducing suum Positive Saperine Poshive ‘Stone obey Negative Taaning Positive pentose Vositve Biossay analysis of the plant Anti-bacterial screening tandard strains of Klebsiella pneumonia, Bacillus aureus, Escherichia coli, Salmonella typhi, Staphylococcus aureus, Providencia Stautti and Pseudomonas aeruginosa were collected from the Microbiology Department, Ahmadu Bello University, Zaria, Nigeria. All cultures were tested for purity. The inocula were prepared by inoculating the stock culture into freshly prepared nutrient broth (Oxoid) and incubating aerobically 4 Aspergiias niger Coa tbc Ti methanol Selanne typi Nigerian Journal of Chemical Research, Vol. 6, 2001Fungicidal activity test . Fresh pure isolates of Candida albicans, Aspergillus niger and Salmonella typhi were ‘obtained from the Microbiology Department of Akmadu Bello University, Zaria. The fungal cultures were maintained on slants of sabourd dextrose agar. The paper disc method was used in ‘this process and the steps involved were the same ‘as those described above for antibacterial screening ‘except that the medium used was potato dextrose ‘agar. However, the cultures and the control ‘experiments were left in the incubator for 72 hours at 27°C. Then the plates were examined and the Determination of the minimum inhibitory concentration (m.1.0.) ‘The tube dilution method"? was in this determination. In a set of five sterile 7.62 em by 1.27 om test tubes, and for each extract, 1.0 inl of the prepared broth was transferred into each ‘Of the test tubes and 1.0 ml of the 400 mg/ml soda elon was andi in tho St tet tube to give a concentration of|299 mg/ml. This ‘was diluted out serially by the dilution method to To” ‘onceniration of exact v/a [aR ORK T O07 concentrations (in g/ml) of 100, 50, Bamscnrens WF ie sive graded 25, 12.5, 6.25, 3.12 and 1.56 respectively. A "romercta stout « loopfial from the culture which showed zone of "krhiacol ir inhibition was introduced into cach of the test "wma eoginss 2s which was, then capped ‘and teft at roar Sép/nocnces aureus aperaturo for 72 hours. The tubes were then Kb memonice $+ddEH exomined for’ the presence or absence of grontblturbidity, The lowest concentration inhibiting growth was taken as the mi.c. and this of Tapinaniivus pines showed the rence of carbrhydrutes, sugars, pentose, sponin glycosides, iatsins, aloes and alkaloids as able 1. It is suggested that dhe tannin content of plant is responsible for its claimed efficacy in ‘ibe treatment of wounds and burns in Zaria, Table 2 shows the result of the ontibacterial screening Cape in millimeters of the diametrical sections of the respective zones of inhibition whereas Table 3 is the result of the m.ic., recorded for the microbes whose growth 45 ‘Again the mic. results, show that the methanol extract of this plant is not just bacteristatic with respect to the organisms under study, but it is also bactericidal. The plant is used locally for the ‘treatment of chest pains among others and this result really provides a scientific basis for the folk medicinal properties of the plant. However, the extract principles responsible for these Nigerian Journal of Chemical Research, Vol. 6, 2001Ahesadu Bello University, Zaria, fan! cidated ane sin being ACKNOWLEDGE We are very grateful to tho this research. REFERENCE: Daziel, 1M., The useful pleois of West Africa, Vol. t, p. 297, Ciowa Agents Colonies, London, 1937. Irvine, R., Woody Plants of Ghana, pp. 476-479, Oxford University Press, London, 1961. Brain, KR, and. Tumer, T.D., The Practical Evaluation of Phytopharmaceuticals, pp. 57 - 63, ‘Wright-Science Technical, Bristol, 1975, Ericsson, H.M. and Shervis, J.C., Acta: bream ‘Scand. Sect, B. Supl, 1971, 257. . Barry, AL, Gracia, F. and Thrupp, L.D., Ames, 3. Clin Path., 1975, 53, 149. Garrod, L..?. aud Weterworth, P.M., Path,, 1971,24, 779. Machido, B.A. and Ado, $.A., J. Pharm, Research and Development, 1999, 4:1, 53. accepted 6/12/2001 received 3/9/2001 Nigerian Journal of Chemical Research, Vol. 6, 2001