PENGEMBANGAN DETEKSI Aeromonas hydrophila PENYEBAB PENYAKIT MOTILE

AEROMONAS SEPTICEMIA (MAS) PADA IKAN GURAME (Osphronemus goramy)

DENGAN METODE CO-AGGLUTINATION 1)
(Development of Detection Aeromonas hydrophila Causative agent of Aeromonas Septicemia
Motile Disease (MAS) on Gourami (Osphronemus goramy)
with Co-Agglutination Method)
Dian Fitria M2), Sukenda3), Munti Yuhana3)

ABSTRACT
This study aims to develop co-agglutination method for detecting and creating
Aeromonas hydrophila diagnostic rapidly used in field or laboratory analysis. Rabbits were
immunized by injection of heat killed A. hydrophila in order to produce polyclonal antibodies.
The purified serum was coupled with Staphylococcus aureus which contained protein A with
a ratio of 1: 1 (v / v) for a co-agglutination reagent. We compared this method with standard
PCR (Polymerase Chain Reaction) for A. hydrophila detection. No agglutination reaction
between reagent with A. salmonicida, A. sobria, Pseudomonas aeruginosa dan Streptococcus
agalactiae. The reaction occurred only against A. hydrophila showed the specificity of the
gourami tissue supernatant. The specificity test of co-agglutination reagents showed that
positive reaction in gourami infected by A. hydrophila within 10-30 seconds, and sensitivity
test had a detection limit of 106 cfu ml–1. Comparison detection method with PCR showed that
positive result of A. hydrophila was located in 209 bp. The occured of A. hydrophila antigen
with co-agglutination or PCR was able to detect the A. hydrophila antigen. This method could
be detected A. hydrophila of internal organ at 12 h pasca infection, but the PCR method can be
detected at 1 h pasca infection. Co-agglutination method has advantage reaction like
specifically, accurately, rapidly and practically to detect A. hydrophila in laboratory and field
observation.
Keywords: Aeromonas hyrophila, gourami (Osphronemus goramy), Co-agglutination, PCR