Time X ln(X) OUR

0 0.6 -0.510826 0.011

1 0.63 -0.462035 0.008
2 0.63 -0.462035 0.084
3 0.76 -0.274437 0.153
4 1.06 0.0582689 0.198
5 1.56 0.4446858 0.273 2.5

6 2.23 0.8020016 0.393

2
7 2.85 1.047319 0.493 f(x) = 0.3262173692x - 1.2193
8 4.15 1.4231083 0.642 1.5
9 5.37 1.6808279 0.915
10 7.59 2.0268316 1.031

ln(X)
1
11 9.4 2.2407097 1.12
12 11.4 2.4336134 1.37 0.5
13 12.22 2.503074 1.58
0
14 13 2.5649494 1.26 2 3 4 5 6
15 13.37 2.5930134 1.58
-0.5
16 14.47 2.6720775 1.26
Tim
17 15.37 2.7324176 1.12
18 16.12 2.7800607 1.2
19 16.18 2.7837759 0.99
20 16.67 2.8136107 0.86
21 17.01 2.8338014 0.9 1.2

1
f(x) = 0.1302142857x - 0.3341
0.8

0.6
OUR

0.4

0.2

0
2 3 4 5 6
Tim
 f(x) = 0.3262173692x - 1.2193371123

3 4 5 6 7 8 9 10 11

Time

f(x) = 0.1302142857x - 0.3341428571

3 4 5 6 7 8 9 10 11
Time
Dilution Rate (h-1) Substrate Conc. (mg/L) Cell Conc. (mg/L) (So-S)/X 1/D
0.1 16.7 366 2.1401639 10
0.2 33.5 407 1.8832924 5
0.3 59.4 408 1.8151961 3.3333333
0.4 101 404 1.730198 2.5
0.5 169 371 1.7008086 2
0.6 298 299 1.6789298 1.6666667
0.7 702 59 1.6610169 1.4285714
2.5

2 f(x) = 0.055496152x + 1.5958099735

1.5

0.5

0
1 2 3 4 5 6 7 8 9 10 11
v ([Eo]=0.015 g/L) (g/(L*min) [S] (g/L) v ([Eo]=0.00875 g/L) (g/(L*min) [S]/v ([Eo]=0.015)
1.14 20 0.67 17.5438596491
0.87 10 0.51 11.4942528736
0.7 6.7 0.41 9.5714285714
0.59 5 0.34 8.4745762712
0.5 4 0.29 8
0.44 3.3 7.5
0.39 2.9 7.4358974359
0.35 2.5 7.1428571429

35

30
f(x) = 1.0071214694x + 9.6547174942
25

20
[Eo]=0.015 g/L
[S]/v

15 f(x) = 0.5953332672x + 5.5970927758 Li near ([Eo]=0.015 g/L)

[Eo]=0.00875 g/L
10 Li near ([Eo]=0.00875 g/L)

0
0 5 10 15 20 25
[S]
[S]/v ([Eo]=0.00875)
29.8507462687
19.6078431373
16.3414634146
14.7058823529
13.7931034483
Substrate Conc. 0.2 M 0.02 M
1/v [I] 1/[S] 1/v [I] 1/[S]
0.22 0 5 0.68 0 50
0.33 0.0012 5 1.02 0.0012 50
0.51 0.0027 5 1.5 0.0022 50
0.76 0.0044 5 1.83 0.0032 50
0.88 0.0061 5 2.04 0.0037 50
1.1 0.008 5 2.72 0.0044 50
1.15 0.0093 5 3.46 0.0059 50

2.5

1.5
1/v

[I]=0
[I]=0.0012
1
[I]=0.0044

0.5

0
0 10 20 30 40 50 60
1/[S]
[I]=0
[I]=0.0012
[I]=0.0044
 V0 (mmol hydrolzed/L/min)
Free enzyme Immobilized enzyme S0 (mol/L) 1/S0 1/V0 (free) 1/V0 (immobilized)
0.083 0.056 0.01 100 12.048193 17.8571428571
0.143 0.098 0.02 50 6.993007 10.2040816327
0.188 0.127 0.03 33.333333 5.3191489 7.874015748
0.222 0.149 0.04 25 4.5045045 6.711409396
0.25 0.168 0.05 20 4 5.9523809524
0.33 0.227 0.1 10 3.030303 4.4052863436
0.408 0.29 0.29 3.4482759 2.4509804 3.4482758621

20
18
f(x) = 0.1487110679x + 2.9281416418
16
14
12
f(x) = 0.099840011x + 2.0295225856
10 Free
1/V

8 Li near (Free)
Immobi l i zed
6 Li near (Immobi l i zed)
4
2
0
0 20 40 60 80 100 120
1/So
v S 1/S 1/v
0.909 20 0.05 1.10011
0.658 10 0.1 1.5197568
0.493 6.67 0.149925 2.0283976
0.4 5 0.2 2.5
0.333 4 0.25 3.003003
0.289 3.33 0.3003003 3.4602076
0.227 2.5 0.4 4.4052863

5
4.5
f(x) = 9.522507801x + 0.6009970404
4
3.5
3
2.5
1/v

2
1.5
1
0.5
0
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45
1/S
[S] (mol/L) v (umol/min) [S] v/[S]
5.00E-04 125 2.00E+03 2.50E+05
140
2.00E-04 125 5.00E+03 6.25E+05
6.00E-05 121 1.67E+04 2.02E+06 120
4.00E-05 111 2.50E+04 2.78E+06 100
3.00E-05 96.5 3.33E+04 3.22E+06
2.00E-05 62.5 5.00E+04 3.13E+06 80
f(x) = - 2.44319313906126E-06x + 82.960609037
1.60E-05 42.7 6.25E+04 2.67E+06

v
60
1.00E-05 13.9 1.00E+05 1.39E+06
8.00E-06 7.5 1.25E+05 9.38E+05 40

20

0
0.00E+00 5.00E+05 1.00E+06 1.50E+06 2.00E+06 2.50E
1/[S]
06126E-06x + 82.960609037

1.50E+06 2.00E+06 2.50E+06 3.00E+06 3.50E+06

1/[S]
[S] (g/L) v (mg/(L*hr)) 1/[S] 1/v
10 5 0.1 0.2
20 7.5 0.05 0.1333333
30 10 0.0333333 0.1
50 12.5 0.02 0.08
60 13.7 0.0166667 0.0729927
80 15 0.0125 0.0666667
90 15 0.0111111 0.0666667
110 12.5 0.0090909 0.08
130 9.5 0.0076923 0.1052632
140 7.5 0.0071429 0.1333333
150 5.7 0.0066667 0.1754386

16 0.25
14
0.2
12 f(x) =

10
0.15
8

1/v
v

6 0.1

4
0.05
2

0 0
0 20 40 60 80 100 120 140 160 0 0.02
[S]
 0.25

0.2
f(x) = 1.5354624231x + 0.0493329479

0.15
1/v

0.1

0.05

0
0 0.02 0.04 0.06 0.08 0.1 0.12
1/[S]
Part a 250
Bead Diameter, Dp (cm) Rate, v (mg/(L*h))
0.1 200
200
0.2 198
0.3 180
150
0.4 140
0.5 100

v
100
0.6 70
0.7 50
0.8 30 50

Part b 0
0 0.1
S0 (mg UA/L) Rate, v (mg/(L*h)) 1/S0 1/v
10 10 0.1 0.1
25 20 0.04 0.05
50 30 0.02 0.0333333
100 40 0.01 0.025
200 45 0.005 0.0222222
250 46 0.004 0.0217391

0.12

0.1
f(x) = 0.821655606x + 0.0175363888

0.08

0.06
1/v

0.04

0.02

0
0 0.02 0.04 0.06 0.08 0.1 0.12
1/S
 250

200

150
v

100

50

0
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Dp
 curly v (mmol/(L*h))
[S] (mmol/L) 1/[S] [I] = 0 [I] = 1.26 mM [I] = 1.95 mM
0.25 4 1.02 0.9803922 0.73 1.3698630137 0.56
0.33 3.03030303 1.39 0.7194245 0.87 1.1494252874 0.75
0.4 2.5 1.67 0.5988024 1.09 0.9174311927 0.85
0.5 2 1.89 0.5291005 1.3 0.7692307692 1
0.6 1.666666667 2.08 0.4807692 1.41 0.7092198582 1.28
0.75 1.333333333 2.44 0.4098361 1.82 0.5494505495 1.39
1 1 2.5 0.4 2.17 0.4608294931 1.82
1.7857143 2
1.8
1.3333333
1.6
1.1764706 1.4
1 1.2
0.78125 1

1/[I]
0.7194245 0.8
0.6
0.5494505
0.4
0.2
0
0.5 1 1.5 2 2.5 3 3.5 4 4.5
1/[S]

[I] = 0 Li nea r ([I] = 0) [I] = 1.26

Li nea r ([I] = 1.26) [I] = 1.95 Li near ([I] = 1.95)
4 4.5

26
([I] = 1.95)
RPM KL (m/h) curly v (mg substrate/(L*h)) [S0]b [S]s 1/[S]s
1/curly v
25 0.18 30 200 33.333333 0.03 0.0333333
50 0.24 36 200 50 0.02 0.0277778
100 0.33 40 200 78.787879 0.0126923 0.025
200 0.42 41.5 200 101.19048 0.0098824 0.0240964
300 0.88 44 200 150 0.0066667 0.0227273
400 2.25 45 200 180 0.0055556 0.0222222

0.04

0.035

0.03 f(x) = 0.4433862669x + 0.019593203

0.025

0.02
1/v

0.015

0.01

0.005

0
0 0.005 0.01 0.015 0.02 0.025 0.03 0.035
1/[S]s
t X ΔX/Δt 1/dX/dt
0 0.5 0.075 13.333333
2 0.65 0.275 3.6363636
4 1.2 0.5 2 45
6 2.2 1.15 0.8695652 40
8 4.5 0.9 1.1111111
35
10 6.3 0.725 1.3793103
30
12 7.75 0.025 40
14 7.8 0.075 13.333333 25
16 7.95 20

15

10

0
0 1 2 3 4 5 6
Col umn D
4 5 6 7 8 9
Col umn D