Practical No :

Date :

DDBJ DATABASE

INTRODUCTION

DDBJ Center collects nucleotide sequence data as a member of INSDC (International

Nucleotide Sequence Database Collaboration) and provides freely available
nucleotide sequence data and supercomputer system, to support research activities in
life science. Currently, DDBJ Center is in operation at the National Institute of Genetics
(NIG) in Mishima, Japan with endorsement of MEXT; Japanese Ministry of Education,
Culture, Sports, Science and Technology.

Major activities of DDBJ Center are as follows.

 Construction and operation of INSDC :Providing nucleotide and amino acid

sequence data included in patent applications
 Management and operation of the National Institute of Genetics Supercomputer
System
 Providing services to search and to analyze biological data
 Training course and publication
 In Japan, DDBJ Center internationally contributes as a member of INSDC to
collect and to provide nucleotide sequence data with ENA/EBI in Europe
and NCBI in USA.
 DDBJ Center is officially certified to collect nucleotide sequences from
researchers and to issue the internationally recognized accession number to data
submitters. The accession number issued for each sequence data is unique on the
database and internationally recognized to guarantee the submitter the property
of the submitted and published data. Since DDBJ Center exchanges the released
data with ENA/EBI and NCBI on a daily basis, the three data centers share
virtually the same data at any given time. The virtually unified database is
called INSD; International Nucleotide Sequence Database.
DDBJ collects sequence data mainly from Japanese researchers, but of course
accepts data and issue the accession numbers to researchers in any other
countries. 99% of INSD data from Japanese researchers are submitted through
DDBJ.
Procedure and Exercise
1) Open the link http://www.ddbj.nig.ac.jp/ and click on search and
analysis.
2) In the database search click ARSA and enter accession number J00265.
3) Identify the accession number.
4) Name of organism and gene to which the above sequence belong.
5) What is the length of sequence?
6) Give the lineage of the organism.
7) Enlist the name of authors and journal used for references.
8) How many ‘A’,’T’,’G’ and ‘C’ are present in the sequence.