Tris-glycine SDS-Polyacrylamide Gel loading buffer (2X)

1M Tris-HCl (phH6.8) 1.6 millilitre (ml)

10% SDS 4 millilitre (ml)
Glycerol (100%) 2 millilitre (ml)
B-mercaptoethanol 1 millilitre (ml)
Bromophenol blue 4 milligram (mg)
ddH2O 1.4 millilitre (ml)
Total volume 10 millilitre (ml)
Note: store at 4 degree

SDS Gel loading Buffer

To make 10 mL of 4X stock:

 2.0 ml 1M Tris-HCl pH 6.8

 0.8 g SDS

 4.0 ml 100% glycerol

 0.4 ml 14.7 M β-mercaptoethanol

 1.0 ml 0.5 M EDTA

 8 mg bromophenol Blue

Final concentrations (1X):

 50 mM Tris-HCl pH 6.8

 2% SDS

 10% glycerol

 1% β-mercaptoethanol

 12.5 mM EDTA

 0.02 % bromophenol blue