 Making reagents

a. Tollens reagents
2 mL 5% silver nitrate solution

1. Put into test tube

2. Add 2 drops of 5% NaOH solution

Brown precipitate

1. Add drop by drop of 2% NH4OH solution

2. Shake until dissolved

Tollens reagent (Colorless)

b. Fehling reagents
5 mL Fehling A
1. Put into Erlenmeyer 50 mL
2. Add 5 mL of Fehling B
3. Shake it
Fehling reagent

1. Tollent test
Tollens Reagent
1. Put into 4 test tubes each 1 mL

Test tube 1 Test tube 2 Test tube 3 Test tube 4

1. Add 2 drops of 1. Add 2 drops of 1. Add 2 drops of 1. Add 2 drops of

benzaldehyde acetone cyclohexanone formalin
2. Shake it 2. Shake it 2. Shake it 2. Shake it
3. Wait for 10 3. Wait for 10 3. Wait for 10 3. Wait for 10
minutes minutes minutes minutes
4. Place in hot 4. Place in hot 4. Place in hot 4. Place in hot
water (35-500C) water (35-500C) water (35-500C) water (35-500C)
for 5 minutes (if for 5 minutes (if for 5 minutes (if for 5 minutes (if
there is no there is no there is no there is no
change) change) change) change)
Result Result Result Result
2. Fehling and Benedict test
 Fehling test
Test tube 1 Test tube 2

1. Add 2,5 mL Fehling reagent 1. Add 2,5 mL Fehling reagent

2. Add 2 drops of formaldehyde 2. Add 2 drops of acetone
3. Place in boiling water 3. Place in boiling water
4. Wait 10 - 15 minutes 4. Wait 10 - 15 minutes
5. Observed 5. Observed
Observation results Observation results

Test tube 3
1. Add 2,5 mL Fehling reagent
2. Add 2 drops of cyclohexanone
3. Place in boiling water
4. Wait 10 - 15 minutes
5. Observed
Observation results

 Benedict test
Test tube 1 Test tube 2

1. Add 2,5 mL benedict reagent 1. Add 2,5 mL benedict reagent

2. Add 5 drops of formaldehyde
3. Place in boiling water
4. Wait 10 - 15 minutes
5. Observed
Observation results
2. Add 3 drops of acetone
3. Place in boiling water
4. Wait 10 - 15 minutes
5. Observed
Observation results

Test tube 3
1. Add 2,5 mL benedict reagent
2. Add 3 drops of cyclohexanone
3. Place in boiling water
4. Wait 10 - 15 minutes
5. Observed
Observation results
3. Bisulfite Addition
5 mL of saturated NaHSO3

1. Put into test tube

2. Chill the solution in ice water (write
the temperature)
3. Add 2,5 mL of acetone drp-by-drop
while shaking
4. Wait for 5 minutes
5. Added 10 mL of ethanol
6. Filtered with filter funnels

Hablur Filtrate

1. Add a 30 drops of concentrated HCl

2. Write down the change that occur
Observation results

4. Testing with phenylhidrazine

a. Benzaldehyde test
2,5 mL of phenylhidrazine

1. Put into test tube

2. Added 10 drops of benzaldehyde
3. Close the test tube and shake it firmly
1-2 minutes until become hablur
4. Filtered with filter funnels

Hablur Filtrate

1. Wash with a little cold water

2. Hablur it again with a little ethanol or
methanol
3. Dry it
4. Determined its melting point
Observation results
b. Cyclohexanone test

2,5 mL of phenylhidrazine

1. Put into test tube

2. Added 10 drops of cyclohexanone
3. Close the test tube and shake it firmly
1-2 minutes until become hablur
4. Filtered with filter funnels

Hablur Filtrate

1. Wash with a little cold water

2. Hablur it again with a little ethanol or
methanol
3. Dry it
4. Determined its melting point
Observation results

5. Haloform reaction
 Test tube I
3 mL of NaOH 5% solution

1. Put into test tube

2. Added 5 drops of acetone
3. Added iodine solution 2 mL while
shaked until iodine solution
become colorless
Yellow precipitate
4. Smelled
Smell
 Test tube II
3 mL of NaOH 5% solution

1. Put into test tube

2. Added 5 drops of isopropil alcohol
3. Added iodine solution 2 mL while
shaked until iodine solution
become colorless
Yellow precipitate

4. Smelled
Smell

6. Aldol condensation
4 mL of NaOH 1% solution

1. Put into test tube

2. Added 3 drops of acetaldehyde
3. Shaked
4. Heated for 3 minutes
5. Smelled
Roncidness
7. Carboxylic identification
a. 5 mL of CH3COOH solution

1. Put into test tube

2. Added 3 mL of KMnO4 solution
3. Observed the changes that occur
Experiment results

5 mL of NaOH 5% solution

4. Put into test tube

5. Added 3 drops of FeCl3 5%
solution
Red solution

3. Heated
Brownish red precipitate

2. Filtered with filter funnels

Filtrate Residue

1. Show that is does not contains

ferric ions with K4FeCN6
Color change occurs