Control Gram Stain Endospore Stain Biochemical Test Biochemical Test

1: Catalase Test 2: Oxidase Test

Escherichia coli Negative Endospore absent Positive Negative
Enterobacter Negative Endospore absent Positive Negative
aerogenes
Klebsilla Negative Endospore absent Positive Negative
pneumoniae

Control Biochemical Test Biochemical Test Biochemical Test Biochemical Test

3: MAC agar plate 4: MSA Plate 5: Gelatin 6: Indole
hydrolysis production
Escherichia coli Positive -, No Growth Negative Positive
Enterobacter Positive -, No Growth Negative Negative
aerogenes
Klebsilla Positive -, No Growth Negative Negative
pneumoniae

Control Biochemical Test Biochemical Test Biochemical Test Biochemical Test

7: Motility-Indole 8: Methyl Red 9: Voges 10: Nitrate
Ornithine Test Proskauer Test Reduction Test
Decarboxylation
Test
Escherichia coli Positive Positive Negative Positive
Enterobacter M & O positive Negative Positive Positive
aerogenes I-Negative
Klebsilla Negative Negative Positive Positive
pneumoniae

Control Biochemical Test Biochemical Test Biochemical Test Biochemical Test

11: Simmon’s 12: Starch 13: Triple Sugar 14: Urease Test
Citrate Agar Hydrolysis Iron Agar Test
Slant
Escherichia coli Negatative Negative Acid/ Gas.Positive Negative
Enterobacter Variable, + Negative Acid/Gas, Positive Negative
aerogenes
Klebsilla Positive Negative A/A Positive
pneumoniae