Diagram 3 shows an experiment to study the effect of temperature on the growth of

bacteria, Bacillus subtilis.

Rajah 3 menunjukkan satu eksperimen untuk mengkaji kesan suhu
terhadap pertumbuhan bakteria, Bacillus subtilis.
K L M

0 0
Temperature:5 C Temperature:37 C 0
Temperature:70 C
0 0
Suhu : 5 C Suhu : 37 C 0
Suhu : 70 C

3 3
5 cm nutrient broth + 1 cm bacteria culture
3 3
5 cm bubur nutrien + 1 cm kultur bakteria

Diagram 3
Rajah 3

After three days, the appearance of the nutrient broth in each test tube is
oserved. The result recorded is shown in table 3.
Selepas tiga hari, keadaan bubur nutrien dalam setiap tabung uji
diperhatikan . Keputusan direkodkan dalam Jadual 3
0
Test tube Temperature, C Appearance of nutrient broth
0
Tabung uji Suhu, C Keadaan bubur nutrien
Slightly cloudy
K 5
Sedikit keruh
Cloudy
L 37
Keruh
Slightly cloudy
M 70
Sedikit keruh
Table 3
Jadual 3

(a) State the hypothesis for this experiment

Nyatakan hipotesis untuk eksperimen ini.

_____________________________________________________
_
 _____________________________________________________
_

a) _____________________________________________________
_
1 [1 mark]
[1 markah] For
(b) State the variable in this experiment. Examiner’s
Nyatakan pemboleh ubah dalam eksperimen ini. Use

(i) Constant variable

Pembolehubah dimalarkan.

_______________________________________________

(ii) Responding variable

Pembolehubah bergerak balas.

_______________________________________________ 3(b)3(
[2 marks]
[2 markah] 2

(c) Based on Table 3, what is the optimum temperature for the growth of bacteria?
Berdasarkan Jadual 3,apakah suhu optimum untuk pertumbuhan bakeria ?
3(c)
________________________________________________________________________
[1 mark]
[1 markah] 1

(d) Based on this experiment, state the operational definition for the growth of
bacteria.
Berdasarkan eksperimen ini, nyatakan definisi secara operasi bagi pertumbuhan
bakteria.

___________________________________________________________

___________________________________________________________
3(d)
___________________________________________________________
[1 mark] 1
[1 markah]
Total

A3

5
Jawapan:

3 (a) Able to state the hypothesis for this experiment correctly.

Sample answer:
0
If the temperature is 37 C, then the nutrient broth is cloudy. // 1
0
If the temperature is 5/70 C, then the nutrient broth is slightly
cloudy. //
If the temperature is low/high, then the growth of bacteria is
slow/ retarded. //
0
The optimum temperature for the growth of bacteria is 37 C.
At the optimum temperature the nutrient broth is cloudly.
0
Jika suhu 37 C, maka keadaan bubur nutrient keruh //
0
Jika suhu 5/70 C, maka keadaan bubur nutrient sedikit keruh //
Jika suhu rendah/tinggi, maka pertumbuhan bakteria 1
sedikit/terencat.
0
Suhu optimum bagi pertumbuhan bakteria ialah 37 C. //
Pada suhu optimum keadaan bubur nutrient keruh 1
[Any acceptable answer]
(b)(i) Able to state the constant variable correctly

Sample answer

Volume of nutrient broth // volume of bacteria culture // type of 1 1

bacteria
Isipadu bubur nutrient // isipadu kultur bakteria // jenis bakteria

[Any acceptable answer]

(ii) Able to state the responding variable correctly

Sample answer
Appearance of nutrient broth // the growth of bacteria
Keadaan bubur nutrien // pertumbuhan bakteria 1 1
/mikroorganisma

(c) Able to state the optimum temperature for the growth of

bacteriacorrectly

Sample answer
0
37 C 1 1
(d)
Able to state the operational definition for the growth of
bacteria correctly

Sample answer
The growth of bacteria is a condition shown by the cloudiness of
the nutrient broth
Pertumbuhan bakteria ialah keadaan yang ditunjukkan oleh 1 1
kekeruhan bubur nutrien.
Pertumbuhan bakteria ialah suatu proses yang menyebabkan
bubur nutrien menjadi keruh.

TOTAL 5