Claudia Schäfer, CIR, Penzberg
4486

MODULAR ANALYTICS E170, cobas e 601, cobas e 602,

Elecsys® 2010, cobas e 411
Elecsys® Dilution Recommendations
Reagent type Test Recommended dilution Concentration of diluted
ratios sample must be
Thyroid Function FT3 III ∅
Test FT4 II ∅
T3 ∅
T4 ∅
TSH 1:10 with Diluent MA > 10 µIU/ml
T-uptake ∅
TG II 1:10 with Diluent MA > 50 ng/ml
Anti-TG @
Anti-TPO @ 1:5 with Diluent Universal > 200 IU/ml
manually
Anti-TSHR 1:5 or 1:10 with Anti-TSHR > 4 IU/l
negative human sera*
Calcitonin 1:100 with Diluent MA > 20 pg/ml
Cardiac CK-MB (STAT) 1:2 with Diluent MA > 50 ng/ml
Digoxin 1:2 with Diluent Universal > 2.5 ng/ml
>3.2 nmol/l
Digitoxin 1:2 with Diluent Universal > 30 ng/ml
> 39 nmol/l
Myoglobin (STAT) 1:10 with Diluent Universal > 50 ng/ml
proBNP II (STAT only 1:2 with Diluent Universal > 15000 pg/ml
cobas e 601/2) > 1770 pmol/l
Troponin I (STAT) 1:10 with Diluent MA > 3 µg/l
> 3 ng/ml
Troponin T hs (STAT ) 1:10 with Diluent MA > 1000 ng/l

Autodilution possible for bold mentioned dilution ratios

∅ Dilution not necessary due to the broad measuring range or not possible e.g. can not be diluted because of changing in the concentration of
the binding proteins alters this equilibrium.

@ The autoantibodies are heterogeneous and this gives rise to non-linear dilution phenomena.

* Please check the method sheet.

STAT for Elecsys 2010, cobas e 411, cobas e 601 (SW version 04-03 onwards) and cobas e 602

# Diluent Universal can be used to dilute the samples.

♣ Auto-dilution is not possible as assay uses a three-step method.

∞ Use Elecsys® Diluent Universal for automatic sample predilution.

∇ Autodilution not possible.

Diluent ratio Version 6.3 Aug. 2016 1/7

Claudia Schäfer, CIR, Penzberg
4486

MODULAR ANALYTICS E170, cobas e 601, cobas e 602,

Elecsys® 2010, cobas e 411
Elecsys® Dilution Recommendations
Reagent type Test Recommended dilution Concentration of diluted
ratios sample must be
Hormones ACTH ∅
AMH 1:2 with Diluent Universal 2 > 10 ng/ml

Cortisol II 1:10 with Diluent Universal > 50 nmol/l

2 > 1.8 µg/dl
DHEA-S 1:5 with low concentrated > 1.5 µmol/l
human sera* > 45 µg/dl
Estradiol III 1:10 with Diluent MA > 881 pmol/l
> 240 pg/ml
FSH ∅
HCG+β β 1:100 with Diluent > 100 mIU/ml
HCG (STAT) Universal*
LH ∅
Progesterone III 1:10 with Diluent Estradiol / > 6 nmol/l
Progesterone ∇ * > 2 ng/ml
Prolactin II 1:10 with Diluent Universal > 50 µIU/ml,
> 2.4 ng/ml
SHBG 1:10 with Diluent MA > 20 nmol/l
Testosterone II ∅
Diabetes C-Peptide 1:10 with Diluent MA > 4 ng/ml
(serum and urine) > 1.3 nmol/l
Insulin ∅
Preeclampsia PlGF ∅
sFLT-1 ∅*
Down`s Free ßhCG 1:10 with Diluent Universal > 19 IU/l
syndrome PAPP-A 1:10 with Diluent Universal > 500 mIU/l
screening
Tumor Marker AFP 1:50 with Diluent Universal > 20 U/ml,
> 24 ng/ml
CA 125 II 1:5 with Diluent Universal > 1000 U/ml
CA 15-3 II ∞ 1:10 with Diluent Universal > 30 U/ml
(only 1:10 possible on E170 /
cobas e 601/2)
CA 19-9 1:10 with Diluent Universal > 50 U/ml
CA 72-4 1:2 with Diluent Universal > 150 U/ml
CEA 1:50 with Diluent Universal > 20 ng/ml
Cyfra 21-1 1:2 with Diluent Universal > 250 ng/ml
HE4 1:20 with Diluent MA > 75 pmol/l
NSE 1:2 with diluent NSE ∇ > 50 ng/ml
ProGRP 1:10 with Diluent MA > 500 pg/ml
Free PSA ∅
Total PSA 1:50 with Diluent Universal > 2 ng/ml
S 100 1:5 with S100 analyt free > 1 µg/l
human serum or CalSet 1
SCC 1:20 with Diluent Universal > 3 ng/mL
Anaemia Vitamin B12 II 1:2♣ with Diluent Universal > 1000 pg/ml,
>738 pmol/l
Ferritin 1:50 with Diluent Universal > 40 ng/ml

Diluent ratio Version 6.2 Nov. 2015 2/7

Claudia Schäfer, CIR, Penzberg
4486

MODULAR ANALYTICS E170, cobas e 601, cobas e 602,

Elecsys® 2010, cobas e 411
Elecsys® Dilution Recommendations
Reagent type Test Recommended dilution Concentration of diluted
ratios sample must be
Folate III 1:2♣ with Diluent Universal > 10 ng/ml,
> 22.7 nmol/l
Folate RBC 1:2 with 0.2% ascorbic acid > 602 nmol/l
solution > 265 ng/ml
Bone ß-CrossLaps ∅
hGH 1:2 with Diluent Universal 2 > 25 ng/ml
N-MID Osteocalcin 1:5 with Diluent Universal > 60 ng/ml
Total P1NP 1:2 with Diluent Universal > 100 µg/l
PTH (STAT) ∅
PTH (1-84) 1:2 with Diluent Universal > 1150 pg/nl
> 122 pmol/l
Vitamin D total 1:2 ♣ with Diluent Universal > 35 ng/ml
> 87.5 nmol/l
Varia IgE II 1:20 with Diluent Universal > 125 IU/ml,
> 300 ng/ml
Rheumatoid Anti-CCP 1:2 to 1:5 with neg. human
Arthritis serum pool*@
Sepsis Brahms PCT 1:4 with neg. human sera > 1.0 ng/ml
IL 6 1:10 with Diluent MA > 50 pg/ml
ISD Cyclosporine 1:3 with Diluent Universal > 500 ng/ml
Everolimus 1:2 with Diluent Universal or > 12 ng/mL
Diluent Universal 2
Sirolimus 1:2 with Diluent Universal or > 12 ng/mL
Diluent Universal 2
Tacrolimus 1:3 with Diluent Universal > 5 ng/ml
Infectious Anti-HAV with Diluent Hepatitis A ∇ > 20 IU/l
Diseases Anti-HAV IgM ∞ #
Anti-HBc #*
Anti-HBc IgM ∞ #
Anti-HBe #*
HBeAg #*
Anti-HBs II 1:100 with Diluent Universal > 10 IU/ml
HBsAg II *
HBsAg II quant II 1:100 or 1:400 depending
on the used system with
Diluent HepB*
Anti-HCV II *
STDs HIV Ag *
HIV combi PT *
HTLV-I/II *
Syphilis *
Torch CMV IgG 1:20 with Diluent Universal ≥ 15 U/ml
CMV IgG Avidity 1:20 with Diluent Universal
1:2 with Dil CMV Av*
CMV IgM ∞ *
HSV-1 IgG *
HSV-2 IgG *
Rubella IgG 1:20 with Diluent Universal > 10 IU/ml

Diluent ratio Version 6.2 Nov. 2015 3/7

Claudia Schäfer, CIR, Penzberg
4486

MODULAR ANALYTICS E170, cobas e 601, cobas e 602,

Elecsys® 2010, cobas e 411
Elecsys® Dilution Recommendations
Reagent type Test Recommended dilution Concentration of diluted
ratios sample must be
Rubella IgM ∞ *
Toxo IgG 1:20 with Diluent Universal > 3 IU/ml
Toxo IgG Avidity 1:2 with Diluent Universal
1:2 with Dil Toxo Av*
Toxo IgM ∞ #*

Diluent ratio Version 6.2 Nov. 2015 4/7

Claudia Schäfer, CIR, Penzberg
4486

MODULAR ANALYTICS E170, cobas e 601, cobas e 602,

Elecsys® 2010, cobas e 411
Elecsys® Dilution Recommendations

Used Documents:
Assay Method Sheet date / version
FT3 III 1212/01
FT4 II 1212/01
T3 0811/20
T4 1011/16
TSH 0911/20
T-uptake 1111/18
TG II 1014/02
Anti-TG 0911/02
Anti-TPO 0811/02
Anti-TSHR 0911/09
Calcitonin 0513/02
CK-MB + STAT 0812/03
Myoglobin + STAT 0812/17
proBNP II + STAT 0512/07
Troponin I + STAT 0712/05
Troponin T hs + STAT 0512/07
Digoxin 0512/18
Digitoxin 0612/11
HCG+β + HCG STAT 0412/15
ACTH 1211/07
AMH 0714/01
Cortisol II 0615/01
DHEA-S 0912/16
Estradiol III 1114/01
FSH 0412/18
LH 0412/18
Progesterone III 0316/01
Prolactin II 0312/05
SHBG 0512/10
Testosterone II 0512/06
C-Peptide 0412/08
Insulin 0412/13
PlGF 0512/05
sFLT-1 0512/05
AFP 0911/10
CA 125 II 1014/01
CA 15-3 II 0512/16
CA 19-9 0911/20
CA 72-4 0312/10
CEA 0512/20
Cyfra 21-1 0812/15
HE4 0612/05
NSE 0812/11
free PSA 0211/10
total PSA 0510/07
proGRP 0913/01

Diluent ratio Version6.2 Nov. 2015 5/7

Claudia Schäfer, CIR, Penzberg
4486

MODULAR ANALYTICS E170, cobas e 601, cobas e 602,

Elecsys® 2010, cobas e 411
Elecsys® Dilution Recommendations

Assay Method Sheet date / version

S100 0510/07
SCC 0316/02
Free ßHCG 0812/06
PAPP-A 0411/03
Vitamin B12 II 0515/01
Ferritin 0312/12
Folate III 0712/10
Folate RBC 1011/01
IgE II 0312/06
β-CrossLaps/serum 1211/12
hGH 0211/02
N-MID Osteocalcin 1211/14
PTH + STAT 1012/22
PTH (1-84) 1012/02
total P1NP 0812/09
Vitamin D total 0812/04
Brahms PCT 0712/07
IL-6 0512/05
Cyclosporine 0913/01
Everolimus 0316/01
Sirolimus 0316/01
Tacrolimus 0913/02
Anti-CCP 0611/04
Anti-HAV 0712/09
Anti-HAV IgM 1012/12
Anti-HBc 0610/20
Anti-HBc IgM 0212/14
Anti-HBe 0610/13
HBeAg 0610/15
Anti-HBs II 1015/01
HBsAg II 1112/11
HBsAg II quant II 0415/01
Anti-HCV II 1211/02
HIV Ag 0712/11
HIV combi PT 1212/03
HTLV-I/II 0615/01
Syphilis 0114/01
CMV IgG 1012/06
CMV IgG Avidity 0712/03
CMV IgM 0712/05
HSV-1 IgG 1211/02
HSV-2 IgG 1211/02
Rubella IgG 1012/06
Rubella IgM 1012/07
Toxo IgG 0612/10
Toxo IgG Avidity 0712/03
Toxo IgM 1211/07

Diluent ratio Version 6.2 Nov. 2015 6/7

Claudia Schäfer, CIR, Penzberg
4486

MODULAR ANALYTICS E170, cobas e 601, cobas e 602,

Elecsys® 2010, cobas e 411
Elecsys® Dilution Recommendations

For this document are the contents of the current Elecsys method sheets used.
The used method sheet are listed above.
Original datas are available in the corresponding method sheets.

Diluent ratio Version 6.2 Nov. 2015 7/7