METHODOLOGY

1.Research Design

This study involves the procedure method including the control or the

manipulation of condition or purpose of studying the relative effects of various

treatments applied to test animals. (Diano, 2012).

The procedures of the methodology includes the preparation of materials prior to

making the mosquito coil, grinding, of the ponkan peelings and coconut husk, mixing

of the ingredients, molding if the mixture, and drying of the molded coil.

The experimental phase assures to attain the needed data and findings, and

possible cause-and-affect relationships by conducting experiments to one or more

experimental groups, gathering data, and comparing it to one or more control groups

not receiving the treatment without bias.

The experimentation followed the Complete Randomized Design (CRD), which

is the appropriate design to assure the reliability of the experimentation and also to

determine the effect of the ponkan peelings and other included ingredients to Aedes

aegypti mosquito.

2.Preparation of Materials

Organic mosquito coil out of ponkan peelings used different materials provided

by the researchers. The main material used is the ponkan peelings which is obtained

from some stores and eateries residing at Biñan City, Laguna. Damages or soon to

perish ponkan in the market was also collected for a cheaper price. Some ponkan

peelings was collected from the leftovers in few canteens. Adults Aedes aegypti

mosquito was bought by the researchers at RITM Alabang. The coconut husk which
was also used in the making of the organic mosquito coil was collected in some stores

of coconuts for free. Cornstarch used for binding was bought locally in the market

while the mosquito coil used a ls a molder of the mixture for the organic mosquito

coil was also bought in the market. Other materials like oven, weighing scale,

blender, basin , and water are provided by the researchers from their houses.

3.Setting of the Study

The researchers chose the place where the experimentation and testing was

conducted and completed in a time-bound manner.

The researchers produced a mosquito coil at Jacobo Z. Gonzales Memorial

National High School, Romana Subdivision, Brgy. San Antonio, Biñan City, Laguna,

Region IV-A CALABARZON.

The researchers' testing was conducted at DOST (Department of Science and

Technology) located at Los Baños Laguna.

This study will be conducted during the period of June 2018 to March 2019.

4.Procedures of the Study

a. Peeling of Ponkan

In order for the researcher to peel the ponkan they used for the experiment, the

researcher bought first the ponkan from the market, they prepared it in the table and

started peeling the ponkan by the used of their hands.

 b. Burning of Coconut Husk

Coconut husk which is used in making the mixture is burned until it turns like a

charcoal. It is burned for 1 hour to be more effective.

c. Grinding

The blender used for grinding is in the power 3. The ponkan peelings are grinded

using the blender until it is powderized . The coconut husk was blended separately

after it turned into small pieces using a hammer.

d. Mixing

After grinding, the ground ponkan peelings and the ground coconut husk are

mixed together as the base material. Next, the cornstarch is mixed with a little

amount of water and serves as the binder. Then, put the mixture in a basin and mixed

it thoroughly.

e. Molding

The researchers will mold the organic mosquito coil using the commercialized

mosquito coil itself. The mixed material will be put on the space of the coil and cover

it using metallic foil.

f. Drying

The molded organic mosquito coil is placed in an oven for 3 minutes under a

temperature 75°C. After it solidify, the organic mosquito coil it is dried once again

under the sun to totally dry the product before being used on a test for gathering data

results.
5.Experimental Design and Treatment

The researchers will be having four treatments, each treatment they make

will have 3 replications of mosquito coil. The researchers will compare the burning

rate of organic coil to the inorganic mosquito coil and will be labeled as Treatment

1( T1). The researchers will apply 50% ponkan peelings and 50% coconut husk to the

organic coil and test its efficiency, this will be labeled as Treatment 2 (T2). In

treatment 3 (T3), the researchers will test the efficiency of an organic coil with 75%

ponkan peelings and 50% coconut husk. Lastly, Treatment 4 (T4) is an organic coil

with 100% ponkan peelings and 50 % coconut husk. Each treatment will have these

applications to ensure the efficiency of the organic mosquito coil.

6.Statistical Analysis

This study aims to compare four different mosquito coil to identify which is

the best in terms of exterminating the mosquitoes. The researchers will use two-way

ANOVA (Analysis of Variance) test of significance to get the mean score of the four

treatments. Since the study has 4 dependent variables, the two-way ANOVA can

examine the interactions between these, which will lead to accurate results. Lastly,

the researchers are aiming to determine if there is a statistically significant

difference among the four treatment.