Entamoeba Hystolytica

Trophozoite
(“feeding stage”)
(noninfective stage)
- Clean looking cytoplasm
- Mononucleated nucleus with centrally located
kayosome
- Finger pointing pseudopod
*released one at a time in explosive manner
*conforms to progressive/ unidirectional
movement
- Inclusion: ingested RBC (“hematophagous”)

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 Entamoeba Hystolytica
cyst
(infective stage)
- Mature cyst
- Quadrinucleated (tetra nucleated)
- Thin cystic wall
- Chromatoidal bar : sausage shape/cigar
shaped with rounded end

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 Entamoeba Coli
trophozoite
(infective stage)
- Dirty looking cytoplasm
- Eccentric nucleus
- Blunt shaped pseupod
*Conforms to sluggish/nondirectional motility
- Inclusion : yeast/bacteria/debris

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 Entamoeba Coli
cyst
(infective stage)
- Mature cyst
- Number of nucleus is 8
- Thick cystic wall
- Chromatoidal bar : Broomstick with
splintered ends
(Note: if the number of nuclei is > 4, mostlikely it
is E. coli cyst)

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 Iodamoeba Butschlii
Cyst
- Mononucleated cyst (1 nucleaus)
- Large I2 stained glycogen vacuole

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 Chilomastix mesnili
Cyst
- Small protuberance (nipple)
- American Lemon shape
- Mononucleated cyst

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 Giardia Lamblia
trophozoite
- Ventral sucking disc (attachment)
- binucleated
- 4 pairs of flagella
*falling leaf motility

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 Giardia Lamblia
Cyst
- Infective stage
- Tetra-nucleated
- Axoneme

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 Endolimax nana
-

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 Balantidium coli
trophozoite
(motile stage)
- Cytosome = mouth
- Cilia – confers thrown ball motility
(rolling motion)
- Micronucleus (with DNA)
- Macronucleus (kidney/bean shaped)
- Contractile Vacuoles
- Cytopyge = anus

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 Balantidium coli
Cyst
(nonmotile/infective stage)
- Cilia within a cystic wall
- Micronucleus
- Macronucleus

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 Blastocystic hominis
(vacuolar form)
- 2-4 nuclei pushed towards periphery
- Large Vacuole

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 Fat Globule
- Cyst like structure
- Increased in cases of “steatorrhea”
(increased fats in stool)
- Fecal artifacts (pseudoparasite)

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 Vegetable cells
- Vegetable remnants

(air bubble- represents trapped air, indicates

improper placement of cover slip)

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 Muscle Fiber
- Rectangular
- Non striated form
- “creatorrhea”
(presence of too much muscle fibers in stool)

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 Vegetable Fiber
- Food remnant
- Usually mistaken as larva of nematode

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 Vegetable Spiral
- Food remnant

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