#3

Characterization of new
strains of Hortaea
werneckii from salt
marshes of Egypt
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

Hortaea werneckii
❖ Phylum Ascomycota; Order Capnodiales

❖ Synonyms: Exophiala werneckii

Phaeoannellomyces werneckii
Cladosporium werneckii

❖ Superficial mycoses

❖ Saprophytic fungus (feeds on decaying

matter) found in tropical/sub-tropical
soils

❖ Halotolerant (favors high salinity)

 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

Hortaea werneckii
❖ Switches between yeast and
hyphal phase

❖ dematiaceous filamentous

❖ Isolated from different marine

habitats
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

Hortaea werneckii
Causative agent of Tinea Nigra
❖ Characterized by brown-black
nonscaly macules

❖ Usually asymptomatic

❖ Confined on the Stratum corneum of the skin

❖ Common on palms of the hand and soles of the

feet

❖ Commonly mistaken for malignant melanoma

 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

Hortaea werneckii
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

Hortaea werneckii
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

Hortaea werneckii
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

Hortaea werneckii
❖ Lab Diagnosis

• KOH Mount (Long, strongly

dematiaceous branching,
septate hyphae & elongated
budding cells)
❖ Treatment

• Whitfield’s Ointment
• 5-10% Salicylic Acid, Tincture of Iodine
• Azoles (e.g. ketoconazole, econazole, miconazole)
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

METHODS
➢ Sample Collection

➢ Northern Egypt

➢ Sealed Glass Jars

 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

METHODS
➢ Sample Collection

➢ Northern Egypt
➢ City of Gamasa

Salt Marsh
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

METHODS
➢ Isolation (saline enriched liquid medium)

➢ Molecular Identification

➢ Biochemical Characterization
➢ Nutritional Characteristics
➢ Enzyme tests (i.e. Catalase, Urease, Lipase, Protease,
Amylase, Cellulase)

➢ Microscopic Characterization
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS
➢ Molecular Identification

➢ EGYNDA08
➢ EGYNDA16
➢ EGYNDA90
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS
➢ Molecular Identification
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS
➢ Biochemical
Characterization
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS
➢ Biochemical Characterization
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS
➢ Biochemical Characterization
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS
➢ Biochemical Characterization
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS
➢ Microscopic Characterization
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS

EGYNDA08
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS EGYNDA08
➢ Microscopic Characterization
(A)
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS EGYNDA08
➢ Microscopic Characterization
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS EGYNDA08
➢ Microscopic Characterization
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS EGYNDA08
➢ Microscopic Characterization
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS

EGYNDA16
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS EGYNDA16
➢ Microscopic Characterization
(A)
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

EGYNDA16
RESULTS
➢ Microscopic Characterization
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS EGYNDA16
➢ Microscopic Characterization
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS

EGYNDA90
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS EGYNDA90
➢ Microscopic Characterization
(A)
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

RESULTS
EGYNDA90
➢ Microscopic Characterization
 Characterization of new strains of
Hortaea werneckii from salt marshes of #3
Egypt

CONCLUSION

➢ EGYNDA08
➢ EGYNDA16
➢ EGYNDA90

➢ First report describing the organism in Egypt

 #3

Characterization of new strains of

Hortaea werneckii from salt marshes
of Egypt

‫توصيف سالالت جديدة من‬

Hortaea werneckii ‫من األهوار‬
‫المالحة في مصر‬
 #3

References / ‫ المراجع‬:

https://www.sciencedirect.com/science/article/pii/S2314808X16300550

http://thunderhouse4-yuri.blogspot.com/2014/03/hortaea-werneckii.html

https://ensia.com/articles/can-software-save-salt-marshes/

https://www.healthline.com/health/tinea-nigra

http://thunderhouse4-yuri.blogspot.com/2014/03/hortaea-werneckii.html