AR226-2747 WL

E. I. du Pont, de Nemours and Company

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Haskell Laboratory for Toxicology and Industrial Medicine

HASKELL LABORATORY REPORT NO. 129-68

Material Tested: Ammonium Perfluorononanoate H. kell No.: 5490

Material Submitted by: Plastics Department Other Code
Fluorocarbons Division

ACUTE ORAL TEST

Procedure: The test material was administered, as a solution in water, by intragastric intubation to young adult ChR-CD
male rats in single doses. Survivors were sacrificed 14-19 days later.

Results:
Solution Dose Weight When Killed L.W./B.W,
% "«/kg Mortalitv* Toxic Signs Bodv Liver X ICO ALP
15 2250 D - l d. Lethal Doses: polyuria, belly-
10 1500 D » l d. crawling, pallor, salivation, half- -
10 1000 D - 3 d. closed eyes, incoordination, 228
15 670 D - 1 d. ruffled fur, diarrhea, continuous « 12.10 187 mg/kg
10 450 D - 1 d. weight loss, emaciation
10 300 D - 6 d. 184 10.48 5.70
2 187 D - 5 d. Nonlethal Doses: pallor, saliva- 185 13.50 7.30
2 130 S - 19 d. tion, polyuria, chewing-motions, 143 13.90 9.92
2 90 S - 14 d. on day of dosing at 26 mg/kg and 178 15.21 8.54
1 60 S - 14 d. above; weight loss for 15, 12, and 177 13.91 7.86
1 40 S - 14 d. 9 days at 130, 90, and 60 mg/kg, 277 23.73 8.57
1 26 S - 14 d. respectively; slight initial 365 2b. 90 7.37
0.1 17 S - 14 d. weight losses at 40 and 26 mg/kg; 321 25.07 7.81
0.1 12 S - 14 d. no clinical signs observed below 316 19.53 6.18
0.1 7.7 S - 14 d. 26 mg/kg 295 20.78 7.04
0,1 5.1 S 14 d. 334 17.42 5.21
0.02 3.4 S - 14 d. 308 14.00 4.54
0.02 2.3 s - 14 d. 331 15.40 4.65
0.02 1.5 s - 14 d. 323 14.70 4.55
0.02 1.0 s 14 d. 348 15.30 4.40

h-*
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1
Controls 352-377 15 .89 4.29-5.11

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* D - ( ) d. Found dead ( ) days after dosing; S - ( ) d. Sacrificed < ) days after dosing. containTSCACBl
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Summary : Ammonium perfluoronanoate

T-- ~~r.......... moderately toxic for the male rat when administered n r » n » ,,
.. símate Let!al Dose (ALD) b ____
^. ! ^ !. ^ lmate.Let.1’,'1 JDOSe < M ? ') bf * "° ^ 8 H n g ? k g=>■
. _ -----------—
Based on- clinical signs, c ue
thev.uui|iuuiiu
compound a pappears
p e a r s t otoa c
act
t asasa an gastro-'
astr 6S’
- *- and
intestinal irritant J “■ produce
to “1 *
heavy 'livers.
* IP hi
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highest n n - o f f a r f 11 lawol
"no-effect” v uthis
level for u ; ~ increased
_____ i i •___ weicht
liver _ . , . and.
increased li\ >r/body weight ratio is 3.4 mg/kg of body weight.

Report by:
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Elizabeth N. Miles
Oral Toxicology Section

ilienry Sherman
Chief, Oral Toxicology Section

ENM:HS/jch
Date: June 13. 196ft

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