TCVN TIEU CHUAN QUOCGIA TCVN 5255 : 2009 Xuat ban lan 2 CHAT LUGNG BAT _ PHUGONG PHAP XAC DINH HAM LUGNG NITG DE TIEU Soil quality - Method for the determination of bio-available nitrogen HA NOI - 2009TCVN 5255 : 2009 Loi noi dau TCVN 5255 : 2009 thay thé cho TCVN 5255 1990 TCVN 5255 : 2009 do Ban ky thuat tidu chudn quéc gia TCVN/TC 190 Chat long dat bién soan, Tang cuc Tidu chugn Bo lvang Chat lugng dé nghj, 86 Khoa hee va Gang nghé cdng bé. eoee TIEU CHUAN QUOCGIA TCVN 5255 : 2009 EEE Chat lugng dat — X4c dinh ham lugng nit dé tiéu ‘Soil quality - Method for the determination of bio-available nitrogen 1 Pham vi ap dung Tiéu chugn nay qui dinh phuong phap xac dinh ham ludng nite dé tiéu trong dal bang phudng phap chung cat, 2 Tailigu vién dan Cac lai ligu vién din sau rat cn thidt cho vide 4p dung tiéu chudn nay. 86 véi cac tai héu vien dan ght nam cong bé thi ap dyng phién ban duge néu, Dé) vél cdc tal liéu vién dn khong ghi ndm céng bé thi 4p dung phién ban méi nha, bao gém ca cae sila déi, bé sung (néu cd). TCVN 6647 (ISO 11484), Chal luting dat — Xir ly so b6 dt dé phan tich hoa-ly. TCVN 7538-1 (ISO 10381-1), Chat Jugng dat - Lay mau ~ Ph8n 1. Huang daa thiét KE chung toner lay mau, 3. Nguyén ly Ding dung dich kali ciorua 1 moll 62 chiét cac dang nite dé tiéu cia dat, kht nitrat bang hdn hp Devarda va sau dé chung ¢8t dich chiét i natri hydroxyt trong 6 cl micro Kendan. isp phy amonvaac bang dung dich axit boric va xdc dinh ham Iugng nito bang each chudn do vai cae dung dich chudi axct clohydric. 4 Thiét bj va dung cu Si dung cae dung cu thong thydng trong phong thi nghiém va ede thiét bi dung cu sau 4.1. Gan phan tich, c6 sai s6 + 0,0001 g; 4.2. Can ky thuat, cO sai s6 + 0,1 g; 4.3. BQ cat nito micro Kjendalh;TCVN 5255 : 2009 4.4 Phéu loc, duéng kinh tir 8 em dén 10 em; 4.5 Binh tam giae, dung tich 100 mi, 250 mi; 4.6 Pipet bau, cing tien 20 mi sal sé 40,1 mi 4.7 Buret, dung tich 25 ml, noac 50 mI sai 64 = 0,1 mk: 4.8 May lac 4.9 Binh hut dm. 4.10. Binh dinh mu, dung tich 7000 mL 414 COt loc ionit 5 Thu6c thu, hoa ch: 5.1 Hod chat “hi phan tich, ngoai ter trucing hep cé nhiing chi dln riéng, chi ding cae thuée thes tinh khiét phan tich cat hode nude tinh khiél trang dutdng. Bot nhorn (At) Bot déng (Cu): ~ Ac éng teu chudin axit ciohydric (HCI), ~ Cc chi thi mau: phenolttaiein. mety! dé; bromocresol xan. ~ Nuée cat khOng cé nid Loc nutéc ct qua cae ¢6t ionit. 5.2 Dung dich, thudc ther §.2.1 Dung dich kali clorua 1 moll Diing can ky thudt (4.2) cn 74 g kali clorua, thém nutis edt khong cé nite dén 1 000 ml 5.2.2 Chit mau phenolftalein: Diing cn phan tich (4.1) c&n 0.1 g phenolftalein pha thanh 100 mi bang etanol 70% 5.2.3. Chithima hop metyl dé va bromocresol xan. g can phar tich (4.1) can 0,66 9 melyl dd pha thanh 100 mi véi etanol 95 % ~ Sung ean ph’n rich (4 1) edn 6.99 g bromocresol xanh pha thanh 100 m! véi elanc! 95 %. ts thd Trén 2 dung eich chi thi mau thes.TCVN 5255 : 2009 5.2.4 Dung dich axit bone 2 %: Diing can kj thugt (4.2) cn 20 g axit boric cho vao binh dinh mite da ung sn 700 mi nude cat khong cé nitd dang amoni {NH."). Khudy cho tan hét va dé ngudi, chuyén ‘yao binh dinh mite 1000 mi dug san 200 mi chi th| mau hén hgp. Lac tron déu Diéu chinh maj trudng bang cach nhé ting got dung dich natri hydroxit 10 % cho dn khi may cla dung dich edn hei d3 tia. Pha thém nude ct kndng 66 c41 khéng 6 nite dang amoni cho dén vach dinh mifc, Lac déu va chuyén qua dung trong binh kin ¢6 mau t6h 5.2.5 Hn hdp Devarda; trén déu cdc bét kim loai kém, déng, nhom min va khé theo ty 1é khdi lugng $: 50: 45, Bao quan hdn hgp trong binh hut &m. 5.2,6 Dung dich natri hydroxyt (NaOH) 10 mol/l hoae 40 % 5.2.7 Dung dich chuan axit clohydric 0,01 moll: pha tt cdc 6ng tigu chugn bang nue cat khdng co nite dang ameni trong binh dinh mite 1000 mi. 6 Lay mau va chuan bi mau dy mau dat dai dién thee TCVN 7538-1 (ISO 10381-1). XU ly so bo mau dat theo TCVN 6847 (ISO 11464). 7 C&ch tién hanh 7A Chiét Ding can ky thuat (4.2) can 20,0 g mau dat cho vao binh tam giac cé dung tich 250 ml, thém vao 40 mi dung dich kali clorua 1 mol/l. Lac trong 1 gié va Ine ty dich trong. 7.2. Chimg cat nita 7.2.1 L&p b6 chumg cat nite micro Kendan; dudi Sng ngung lanh ngdp trong 20 mi dung dich axit boric 2% dung trong binh tam gid dung tich 100 ml, 7.2.2, Chung cat nit dang amoni ' Ding pipet hul 20,0 m! dich [oc cho vao binh chung eat nite. - Thém knoding 20 ml nuéc cat va vai giot chi th] phenolftalein, - Thém tir tif dung dich natri hydroxyt (5.2.6) d8n khi dung dich xuat hién mau héng. ~ Cho nuée chay qua éng ngung lanh va dun binh chung cat nits, Thdi glan cat khodng 5 phut ké tir ‘shi sdi dich. = Sau khi chung c&t hét amoniac (c6 thé thir bang gidy tam thuée Netle tr8n ludng hai 4 cudi dng Agung fanh) thi ngiing chung cat va chudn dO lugng nite théng qua lugng amoni borat dude tee 7TCVN 5255 : 2009 thanh trong axit boric bang dung dich tiéu chudn axit clohydric 0,01 mol/| cho dén khi mau chuyén ter xanh sang he dé tia 7.2.3. Chung cat nite dang amoni va nitrat Tién nanh nhu 7 2.2 nbung & bude thir ba déng thai thém dung dich natr hydroxy! va thém 0,2 g hén hop Devarda 7.2.4 Chumg cit riéng nite dang amoni nitrat Nita dang nitrat cé thé tink duse 18 hiéu cla nite hai dang amoni cng véi nitrat véi nite dang amoni Ciing c6 thé chumg ct riéng tién hanh hu 7.2.2 nhung sau khi chung cat hét nitd dang amoni thi thay binh tam gite dung 20 ml ax boric kha va thém 0,2 g hén hdp Devarda vao binh chug cat va tiép tue dun binh chung cat cho dén khi hét amoniac. Chudn dé nite qua lugng amoni borat tao thanh trong axit pone bang cae dung dich chudn axit clohydric 0,01 mot. 7.2.5 Chung cat mau trang chung cat mau trang nhut 7 2.2, thay 20,0 mi dich foc bang 20.0 ml dung dich kali clorua + molt 8 Tinh toan két qua Tinh ham (ueng nit dé tiéu ctla dt (X) tinh bing miligam trong 100 g dat theo céng thie sau’ (V- Vb ox 14x 100K eG Trong do V la thé tich dung dich axit clohydric chudn 6 ding khi chudn 66 dich loc, tinh bang mill V, | thé tich dung dich axit clohydric chudn @ ding khi chudin dé mau trang, tinh bang milli fa néng dd ciia axit clohydric tinh béing mot trén lit; G ta khéi luang dt img voi dich les, tinh bang gam: K la hé sf khd tuyat d8i cia dat: 14 Ia phan tit wong cua nite; 100 18 hé sé quy vé 100 gam dat. 9 Cac yéu td can trd 9.1 Bo nity cain dam bao-tuyét déi kin, tranh mat nit. Trude le sis dung cn kiém tra nhu sau Cho vae binh chung cat 20,0 mi dung dich amoni ciorva va sau dé cho 20,0 ml natri hydroxit 49 % ‘chung cat cho dé khi hét amoniac. 8TCVN 5255 : 2009 phdi chudn d6 nat Chuan dé lueng nits hap thu trong bin ntng dung axit bore Néu 86 cat nito tot ! 7,10 mi dén 7,15 mi dung dich axit clohydre 0.41 mot 9.2. Phong chudn bi mau va chung cat khéng duce cé khi amoniac, 9.3. Nube edt st dung king duge 66 Khi mite, cn toe qua edt rontt. 9.4. Phai tién hanh chung cdt mau tréng véi mdi lin xac dinh. 10 Bao cao thir nghiém Bao cao thin hiém bao gém hing théng tin sau: - Vin dan tiéu chudn nay; ~ Bac diém nhan dang mau; Két qua xde dinh nam luang nita d3 téu: = Moi thao tac khong quy dinh trang ti@u chudn nay ho&e dud cor la tuy chon va cac yéu t6 co thé anh meing dn St qua thir aghiém.