Table of Contents

List of Figures .................................................................................................................................................... ii

1 INTRODUCTION .................................................................................... Error! Bookmark not defined.

2 METHODOLOGY ................................................................................... Error! Bookmark not defined.

2.1 IMMUNISATION OF MOUSE ...................................................... Error! Bookmark not defined.

2.2 ISOLATION OF B CELL FROM SPLEEN .................................... Error! Bookmark not defined.

2.3 CULTIVATION OF MYELOMA CELLS ...................................... Error! Bookmark not defined.

2.4 FUSION OF MYELOMA AND B CELLS ..................................... Error! Bookmark not defined.

2.5 HAT MEDIUM ................................................................................ Error! Bookmark not defined.

2.6 SALVAGE PATHWAY .................................................................. Error! Bookmark not defined.

3 IDENTIFICATION AND ISOLATION OF HYBRIDOMA CELLS ...... Error! Bookmark not defined.

3.1 PRECIPITATION OR AGGLUTINATION METHOD ................. Error! Bookmark not defined.

4 IN VITRO OR IN VIVO MULTIPLICATION ........................................ Error! Bookmark not defined.

5 APPLICATIONS OF HYBRIDOMA TECHNOLOGY .......................... Error! Bookmark not defined.

5.1 THERAPY ....................................................................................... Error! Bookmark not defined.

5.2 IMMUNOPURIFICATION ............................................................. Error! Bookmark not defined.

5.3 DIAGNOSIS .................................................................................... Error! Bookmark not defined.

5.4 SEROLOGICAL .............................................................................. Error! Bookmark not defined.

5.5 FUTURE USE ................................................................................. Error! Bookmark not defined.

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 List of Figures

Figure 1: HYBRIDOMA TECHNOLOGY ....................................................... Error! Bookmark not defined.

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