7/1/2020 NetPrimer :: PREMIER Biosoft

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Oligo Analysis

Name : Silvia Hasanah

Description : Gene homo sapiens gastrine

Sequence #1 (5' to 3')

Length : 20

GGGAATACAGGTGCATGCCA

Sequence #2 (5' to 3')

Length : 20

TGCTCAGCCCAAGACTTGTT

Reaction Conditions

Oligo Concentration : 250.0 pM

Monovalent Ion Concentration : 50.0 mM

Free Mg++ Ion Concentration : 1.5 mM

Total Na[+] Equivalent : 204.92 mM

Temperature for Free Energy Calculation : 25.0 °C

Default Analyze

Analysis Results #1: GGGAATACAGGTGCATGCCA

Rating : 77.0 3' end stability : -10.12 kcal/mol

Molecular Wt : 6191.11 ΔH : -153.9 kcal/mol
Tm : 61.32 °C ΔS : -0.4 kcal/°K/mol
GC% : 55.0 5' end ΔG : -9.66 kcal/mol
GC Clamp : 3 Self Dimer ( ΔG) : -12.66 kcal/mol
nmol/A260 : 4.94 Hairpin ( ΔG) : kcal/mol
ug/A260 : 30.6 Repeats (# of pairs) : kcal/mol

ΔG : -35.53 kcal/mol Run (# of bases) : 3 kcal/mol

Analysis Results #2: TGCTCAGCCCAAGACTTGTT

Rating : 88.0 3' end stability : -7.18 kcal/mol

Molecular Wt : 6068.04 ΔH : -150.1 kcal/mol
Tm : 58.1 °C ΔS : -0.39 kcal/°K/mol
GC% : 50.0 5' end ΔG : -8.27 kcal/mol
GC Clamp : 1 Self Dimer ( ΔG) : -6.5 kcal/mol

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7/1/2020 NetPrimer :: PREMIER Biosoft
nmol/A260 : 5.49 Hairpin ( ΔG) : kcal/mol

ug/A260 : 33.34 Repeats (# of pairs) : kcal/mol

Run (# of bases) : 3 kcal/mol
ΔG : -33.79 kcal/mol

Cross Dimer (ΔG) : -4.3 kcal/mol

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