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Immuno Lab .2ppt PDF
Immuno Lab .2ppt PDF
2: Ouchterlony Double
Immunodiffusion
STUDY QUESTIONS
3. You
are provided with antigen A & B, how do you
determine the antigenic similarity?
Answer:
Ouchterlony method can be used to determine the antigenic similarity.
The Ouchterlony method is one method where a concentration of an antibody can
be measured and seen.
A gel is made up and the antigen and antibody diffuse across the gel towards each
other. Where they meet precipitation/cross linking occurs and this can clearly be
seen on the gel.
Ouchterlony double diffusion (ODD) test, in this test both antigen and antibody
are allowed to diffuse into the gel. This technique can be used to test the
similarity between antigens. Antigens from different species are loaded into two
wells and the known antibody is loaded in a third well located between and
slightly below the antigen wells to form a triangle. Depending on the similarity
between the antigens, different geometrical patterns are produced between the
antigen and antiserum wells. The pattern of lines that form can be interpreted to
determine whether the antigens are same or different.
We observed that
A Shows the pattern of identity (the antibodies in the antiserum react with
both the antigens).
B Shows the pattern of partial identity (the antibodies in the antiserum react
more with one of the antigens than the other).
C Shows the pattern of non-identity (none of the antibodies in the antiserum
reacts with antigenic determinants that may be present in both the antigens).