mg Ca in spike Conc.

In 25 ml Absorbance Absorbance
solution (x) minus (0.002) (y) Best Fit line unknown
0 0 0.159 -3.53951837 0.001185999
0.03203 1.28 0.227 5.376 0.40726
0.06406 2.56 0.273
0.09609 3.84 0.344 E2= INTERCEPT(B2:B6,C2:C6)
0.12812 5.12 0.392 E3= MAX(B2:B6)*1.05
F2= H2*E2+I2
C= given - 0.002 F3= H2*E3+I2

Con. Ca in dry
milk sample % by mass
-3.539518369 4.424397961 2.21219898 C12=
D12=
Stdev t value n Stdev=
1.8081769979 3.182 5 Tvalue=

confidence interval @ 95% B18=

2.21219898 + or - 2.5730967333 % by mass of Ca in milk powder

Calcium curve
0.45
0.4
0.35
0.3
Absorbance

0.25 Column C
0.2 Column F
0.15
0.1
0.05
0
-4 -3 -2 -1 0 1 2 3 4 5 6
Concentration in 25 ml
 Slope Intercept
0.045546875 0.1624

TERCEPT(B2:B6,C2:C6) Slope= SLOPE(C2:C6,B2:B6)

AX(B2:B6)*1.05 Intercept= INTERCEPT(C2:C6,B2:B6)

B12*-0.025/0.002*0.1
C12/200*100
STDEV(C2:C6,B2:B6)
From book

((B15*C15)/2.236067977)