1.

Calculate the concentration of methyltestosterone in an ethanolic solution of which

the absorbance in a 1 cm cell at its λmax, 241 nm, was found to be 0.890. The 𝐀𝟏%
𝟏𝐜𝐦
in the B.P. monograph of Methyltestosterone is given as 540 at 241 nm.
𝐴 0.89
𝑐 (𝑔%) = 1% = = 0.00165 𝑔%
𝐴1𝑐𝑚 . 𝑏 540 × 1

2. Calculate the concentration in µg mL-1 of a solution of tryptophan (molecular weight

204.2) in 0.1M hydrochloric acid, giving an absorbance at its λmax 277 nm, of 0.613
in a 4 cm cell. The molar absorptivity at 277 nm is 5432.
𝐴 0.613
𝑐 (𝑀) = = = 2.82 × 10−5 𝑀
𝜀. 𝑏 5432 × 4
𝑔 𝑔
𝑐 ( ) = 𝑐 (𝑀) × 𝑀. 𝑊 = 2.82 × 10−5 × 204.2 = 0.00576
𝐿 𝐿
𝜇𝑔 𝑔 𝜇𝑔
𝑐 ( ) = 𝑐 ( ) × 1000 = 0.00576 × 1000 = 5.76
𝑚𝐿 𝐿 𝑚𝐿

3. Calculate the absorbance of a solution having a %T of 89 at 400 nm.

𝐴 = 2 − log %𝑇 = 2 − log 89 = 0.051

4. A solution contains 4.50 ppm of a colored species. It is found to have an absorbance

of 0.30 at 530 nm in a 2.00 cm cell. Calculate the absorptivity (a) of this species.
𝑔 𝑐(𝑝𝑝𝑚) 4.50 𝑔
𝑐 ( )= = = 0.0045
𝐿 1000 1000 𝐿
𝐴 0.3
𝑎= = = 33.33
𝑏. 𝑐 2 × 0.0045

5. A solution of Co(H2O)2+ has an absorbance of 0.20 at 530 nm in a 1.00 cm cell. ε is

known to be 10 L mol-1 cm-1 what is its concentration?
𝐴 0.20
𝑐 (𝑀) = = = 0.02 𝑀
𝜀. 𝑏 10 × 1

6. The absorbance of an unknown MnO4- solution is 0.50 at 525 nm. When measured
under identical conditions, a 1.0×10-4 M MnO4- is found to have an absorbance of
0.20. Determine the conc. of the unknown.
𝑐𝑢 𝐴𝑢
=
𝑐𝑠 𝐴𝑠
𝑐𝑢 0.5
−4
=
1 × 10 0.2
𝑐𝑢 = 2.5 × 10−4 𝑀
7. Guanosine has a maximum absorbance at 275 nm. ε at 275 = 8400 L mol-1 cm-1 and
the pathlength is 1 cm. Using a spectrophotometer, it was found that at 275 =
0.70. What is the concentration of guanosine in g%? (M.W of guanosine = 283.24).
𝐴 0.7
𝑐 (𝑀) = = = 8.33 × 10−5 𝑀
𝜀. 𝑏 8400 × 1
𝑐 (𝑀) × 𝑀. 𝑊 8.33 × 10−5 × 283.24
𝑐 (𝑔%) = = = 0.0024 𝑔%
10 10

8. There is a substance in a solution (4 g/liter). The length of cuvette is 2 cm and only

50% of a certain light beam is transmitted. What is the specific absorbance of this
substance?
𝐴 = 2 − log %𝑇 = 2 − log 50 = 0.3
𝑔
𝑐 (𝐿 ) 4
𝑐 (𝑔%) = = = 0.4 𝑔%
10 10
𝐴 0.3
𝐴1%
1𝑐𝑚 = = = 0.376
𝑏. 𝑐 2 × 0.4

9. In question (8), what is the % transmittance if the conc. is 2 g/liter?

𝑐2 𝐴2
=
𝑐1 𝐴1
2 𝐴2
=
4 0.3
𝐴2 = 0.15
𝐴2 = 2 − log %𝑇2
0.15 = 2 − log %𝑇2
%𝑇2 = 70.7%

10. In question (8), what is the molar absorptivity if the molecular weight of this
substance is 100?
𝐴1%
1𝑐𝑚 × 𝑀. 𝑊 0.376 × 100
𝜀= = = 3.76 𝐿 𝑚𝑜𝑙𝑒 −1 𝑐𝑚−1
10 10