Science Investigatory Project

Annona Squamosa
leaves extract:
A potential dog gel soap
ingredient for
eradication of ticks
Submitted by:
Abenaza, Azzinette M.
Najial, Nicole Jane C.
Permejo, Jearicko E.

Submitted to:
Mr. Oliver Lagman
(Research Adviser)
 Annona Squamosa leaves extract:
A potential dog gel soap ingredient for eradication of ticks

Background of the Study

Materials
The goal for this experiment is to find out the
efficacy of Sugar Apple (Atis) leaves extract so that it
can be used for preventing the ticks and fleas of the
dog.  Leaves extract of 3 Annona Squamosa (Atis)
We find out too, that our experiment is not
only for dogs but it can be use also for humans
 2 pieces of a Jar
because in this experiment we will come up the
infestation called Pediculosis.
 Alcohol (Green Cross 70% Solution)

 Strainer
Objectives
 Butter cheesecloth

This project aims to do an alternative and

effectiveness dog soap in order to prevent ticks and  Vegetable oil (Hapi Fiesta)
fleas of a dog so that it would be much cheaper for
some dog owner’s.
 Dropper
After spraying, all of the treatment on the
different containers with the specimen inside, we
started to monitor for the effectiveness of all
treatment by observing dead ticks and fleas, from the
container they have placed it on a clean sheet of
paper then every after observation we put it back in
the container. We have determined that the ticks and
fleas are already dead by observing its movement and
by the use of their bare hands.
 Procedure In Trial 2: Using Licealiz 1 out of 4 ticks are dead,
using our new product which is 25 mL 2 out of 4
Wash your hands; soak the leaves to allow ticks are dead and using our new product which
moisture to stay. It is important to pick leaves that are is 50mL 4 out of 4 ticks are dead.
green and moist to allow for easy extraction of the In Trial 3: Using Licealiz 2 out of 4 ticks are dead,
extracts. Get a jar put the leaves and pours enough using our new product which is 25 mL 4 out of 4
alcohol until it covers it. Cover so the alcohol does not ticks are dead and using our new product which
evaporate and shake it up until couple of days or so. is 50mL 4 out of 4 ticks are dead.
After three to seven days, strain the alcohol from the
solids and transfer it to a cover jar. Place the solids Conclusion
back to the original jar, cover with fresh and put the
lid on. After three to seven days strain out the alcohol We conclude that our new product which is
as before from the second extraction. Combine it with Atis Leaves extract a potential dog gel soap is more
the alcohol from the first extraction and filter it all effective in killing ticks unlike to the old product
through butter cheesecloth to remove any remaining named Licealiz because based on our Data and Result
solids. Place the alcohol in an open container and our new product have the higher number of dead ticks
allow it to evaporate for a few days. After the alcohol than the Licealiz so that our new product is more
evaporates you’ll be left with the oils. Put the oils into cheaper than the other one because it is ingredient by
small bottles with eye dropper cap. Atis Leaves that you can buy in Supermarket or
“Palengke” in Filipino.
And test the gel soap for its effectiveness in
killing the ticks and fleas of a dog by spreading the gel Recommendation
soap to its whole body, scrubs it for 2-3 minutes and
drain/cleanse it. And after that we will measure the We recommend our new product to all of you because
amount of ticks and fleas died by using new product
we’ve been prove in our Data and Result that our new
we’ve made and record the data in the data notebook.
product is more effective than the commercial
Data and Result product like Licealiz and it is more cheap because it is
ingredient by Annona Squamosa or Atis leaves extract
After 3 Trial 1 Trial 2 Trial 3 and it can be used for preventing ticks of the dog that
minutes it is the main problem of several Dog’s Owner.
Treatment A: 2/4 1/4 2/4
Licealiz
Treatment B: 3/4 2/4 4/4
Atis Leaves
extract 25
mL
Treatment C: 4/4 4/4 4/4
Atis Leaves
extract 50
mL

This table shows the number of ticks in each trial.

In Trial 1: Using Licealiz 2 out of 4 ticks are dead,

using our new product which is 25 mL 3 out of 4
ticks are dead and using our new product which
is 50mL 4 out of 4 ticks are dead.
Documentation
Documentation Documentation