The autoclave uses steam under pressure to sterilize materials by reaching and maintaining a temperature of at least 121 degrees Celsius for a minimum of 15 minutes, which is hot enough and for a long enough period to kill all microorganisms and their spores. It functions similarly to a pressure cooker by pushing steam into its chamber to increase the pressure and temperature, cooking the internal parts of any microorganisms until they die off and leaving the sterilized items free of germs until potential recontamination.
The autoclave uses steam under pressure to sterilize materials by reaching and maintaining a temperature of at least 121 degrees Celsius for a minimum of 15 minutes, which is hot enough and for a long enough period to kill all microorganisms and their spores. It functions similarly to a pressure cooker by pushing steam into its chamber to increase the pressure and temperature, cooking the internal parts of any microorganisms until they die off and leaving the sterilized items free of germs until potential recontamination.
The autoclave uses steam under pressure to sterilize materials by reaching and maintaining a temperature of at least 121 degrees Celsius for a minimum of 15 minutes, which is hot enough and for a long enough period to kill all microorganisms and their spores. It functions similarly to a pressure cooker by pushing steam into its chamber to increase the pressure and temperature, cooking the internal parts of any microorganisms until they die off and leaving the sterilized items free of germs until potential recontamination.
The autoclave carries out that exact function of sterilizing
materials. It is a machine that uses pressure and steam to reach and maintain a temperature that is too high for any microorganisms or their spores to live. Microorganisms are what most people commonly refer to as germs. These are the bacteria, viruses, fungi, parasites, etc. that are able to cause infections in our bodies. Spores are the environment-resistant form of the microorganisms. Even though they are able to withstand harsher conditions, they still can be killed if extreme conditions are maintained for an extended period of time. Autoclaves are pressure cookers very similar. Once an autoclave is started, steam is pushed into the chamber that contains the items that are being sterilized. As the steam goes in, the pressure and temperature within the chamber are increased. Most autoclaves are set to increase steam pressure until a temperature of at least 121 degrees Celsius is reached (about 250 degrees Fahrenheit). This temperature and pressure will remain at this level for at least 15 minutes. This is a high enough temperature for a long enough period of time to kill any and all microorganisms and their spores.The high temperatures cause the internal parts of the microorganisms to essentially cook. Once the internal parts cannot function in the microorganisms, they will die. The steam and pressure are released and brought down to normal room temperature and pressure after the 15 or more minutes of running. The items that were autoclaved will remain sterile until they are contaminated by new microorganisms. SANA HUSSAIN 2018-BM-069