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Culture Media
Culture Media
Selective Differential
1 Nutrient agar Normal
Cytochrome c. Pseudomonas
Oxidase from enterics Red/Blue filter= Positive
2 MacConkey Agar (/) Intestinal Flora )Bile Lactose Fermenter – Red (EC) Red/ Pink- positive, acidic, intestinal,
Salts and crystal violet lactose fermenter
Non Lactose Fermenter –
Creamy Pale (P, S, Colorless- Negative non lac and alkaline
Pseudomonas)
3 Mannitol Salt Agar Mannitol Salt (/) Salt Tolerant= 7.5% Non-patho- Micrococcus Salt Tolerance Growth = Salt tolerant, G
Plates
Fermentation Salt, -
Patho- Staphylococcus Fermentation of
(/) Staphylococci Mannitol Salts Yellow- Pathogenic
Mannitol Fermenter
(/) G+ Gram positive or Pink- nonpathogenic
Negative
(X) Non-Salt Tolerant
Pathogen
(-) no clear
5 Eosin-Methylene Blue Agar (/) G- EC- purple with black center/
green metallic sheen
(/) Enteric Bacteria
(intestinal)
Klebsiella pneumonia- dark
centered colonies/ green
metallic sheen
Salmonella typhimurium-
colorless colonies
6 Simmon’s Citrate Agar Slant Citrate Carbon Source Citrate carbon source (+) Blue
Utilization Enterobacteriaceae end product CO2
Alkaline pH Turns (-) Green
thiol blue green color
to blue
Hydrogen Sulfide
Prod (GAS)
24 Hours
A Acidic
K Alkaline
NC No change
0
Gas production = anaerobic
+ Black H2SO4
8 Thioglycolate Differentiates
aerobes from
anaerobe and other
classifications
11 Motility Indole Ornithine tube Motility Indole Motility (+) Growth away from stab line
Ornithine
Decarboxylation Ornithine Decarboxylase (+) Purple Media
test. Kovacs
Reagent Indole (+) Red top (kovac)
12 Urea Broth Phenol Red Proteus (+) from Salmonella (-) Hydrolysis of Urea to (+) Pink
indicator make an alkaline pH
(-) No change
13 Nitrate Broth NR 1 Nitrate reduced to (+) Red
NR 2 nitrite (-) no change
(-) Solid