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MICROPLATE READER
Operation Manual
MR-96 Microplate Reader Operation Manual(2.0)
copyright
© 2005 Shenzhen Mindray Bio-medical Electronics Co., Ltd. All rights Reserved.
For this Service Manual, the issued Date is 2006-11 (Version: 2.0).
SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD. (hereinafter called Mindray) owns
the intellectual property rights to this Mindray product and this manual. This manual may refer to information
protected by copyrights or patents and does not convey any license under the patent rights of Mindray, nor the
rights of others. Mindray does not assume any liability arising out of any infringements of patents or other rights
of third parties.
Mindray intends to maintain the contents of this manual as confidential information. Disclosure of the
information in this manual in any manner whatsoever without the written permission of Mindray is strictly
forbidden.
Release, amendment, reproduction, distribution, rent, adaption and translation of this manual in any
manner whatsoever without the written permission of Mindray is strictly forbidden.
, are the registered trademarks or trademarks owned by Mindray in China and other countries.
All other trademarks that appear in this manual are used only for editorial purposes without the intention of
improperly using them. They are the property of their respective owners.
All information contained in this manual is believed to be correct. Mindray shall not be liable for errors
contained herein nor for incidental or consequential damages in connection with the furnishing,
performance, or use of this manual.
Mindray is responsible for safety, reliability and performance of this product only in the condition that:
• all installation operations, expansions, changes, modifications and repairs of this product are conducted by
Mindray authorized personnel;
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MR-96 Microplate Reader Operation Manual(2.0)
• the electrical installation of the relevant room complies with the applicable national and local requirements;
• the product is used in accordance with the instructions for use.
Upon request, Mindray may provide, with compensation, necessary circuit diagrams, calibration illustration list
and other information to help qualified technician to maintain and repair some parts, which Mindray may define
as user serviceable.
Note
This equipment is to be operated only by medical professionals trained and authorized by Mindray or
Mindray-authorized distributors.
Warning
It is important for the hospital or organization that employs this equipment to carry out a reasonable
service/maintenance plan. Neglect of this may result in machine breakdown or injury of human health.
Warranty
Exemptions
Mindray's obligation or liability under this warranty does not include any transportation or other charges
or liability for direct, indirect or consequential damages or delay resulting from the improper use or
application of the product or the use of parts or accessories not approved by Mindray or repairs by
people other than Mindray authorized personnel.
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MR-96 Microplate Reader Operation Manual(2.0)
Return Policy
Return Procedure
In the event that it becomes necessary to return this product or part of this product to Mindray, the
following procedure should be followed:
1. Obtain return authorization: Contact the Mindray Service Department and obtain a Customer Service
Authorization (Mindray) number. The Mindray number must appear on the outside of the shipping
container. Returned shipments will not be accepted if the Mindray number is not clearly visible. Please
provide the model number, serial number, and a brief description of the reason for return.
2. Freight policy: The customer is responsible for freight charges when this product is shipped to Mindray for
service (this includes customs charges).
3. Return address: Please send the part(s) or equipment to the address offered by Customer Service department
Company Contact
Address: Mindray Building, Keji 12th Road South, Hi-tech Industrial Park,
Nanshan,Shenzhen518057,P.R.China,
Safety Precautions
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MR-96 Microplate Reader Operation Manual(2.0)
WARNING Read the statement following the symbol. The statement is alerting
CAUTION Read the statement following the symbol. The statement is alerting you to a
possibility of system damage or unreliable results.
Read the statement following the symbol. The statement is alerting you to
NOTE
information that requires your attention.
The labels attached to some MR-96 panels use symbols with the text to clarify the meaning of the text. The
table below explains the symbols on the labels.
Serial Number
Manufacturer
Date of Manufacture
Graphics
All graphics, including screens and printout, are for illustration purposes only and must not be used for any
other purpose.
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MR-96 Microplate Reader Operation Manual(2.0)
Safety Symbols
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MR-96 Microplate Reader Operation Manual(2.0)
Table of Contents
1. INSTALLATION .............................................................................................................................................................2
2. FUNCTION......................................................................................................................................................................3
3. OPERATION ...................................................................................................................................................................4
4. MAINTENANCE ..........................................................................................................................................................19
4.1. NOTICE...................................................................................................................................................................19
4.2. MAINTENANCE .......................................................................................................................................................19
5. TECHNICAL SPECIFICATIONS...............................................................................................................................19
6. TROUBLESHOOTING................................................................................................................................................20
7. WARNING .....................................................................................................................................................................21
8. PACKAGING ................................................................................................................................................................21
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MR-96 Microplate Reader Operation Manual(2.0)
1. Installation
1.1. Unpacking
When you receive your MR-96, carefully unpack the package and check the device. If you see any
damages, file a claim immediately with your local distributor or our local agency.
Check the MR-96 and accessories against the packing list. If you find anything missing or
The MR-96 should be free of dust, vibration, corrosive and flammable gas, interference by strong
current and magnetic field as well as direct sunlight. Moreover, enough space should be kept
1.3. Connection
Check if the voltage conforms to requirements of the device. Make sure the power jack is
grounded correctly; otherwise you should connect a single ground wire to the Grounding port on
the device.
Connect the printer to the Printer port on the device through a data cable.
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MR-96 Microplate Reader Operation Manual(2.0)
Insert one end of the power cord into the Power Jack on the rear panel of the device and other end
2. Function
2.1. Introduction
The MR-96 Microplate Reader is an 8-channel optical measuring system, which can be used to
measure the density of vertical light. It is a specialized analyzer to hold ELISAs and then take
With the advanced high speed microprocessor in the world and man-machine conversation
design on the software, the MR-96 makes the operation simpler and more convenient, result
Printer Port Serial RS232 Port Power Jack Power On/Off Grounding
Figure 1
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MR-96 Microplate Reader Operation Manual(2.0)
Figure 2
3. Operation
3.1. Power-on
When you place the Power On/Off to ON ( | ), the device will begin self-check and then enter the Main
Menu screen (Figure 3) if everything is normal. After powering on the device, you should wait 10m for it to
preheat, so that each component and the lamp within the device become steady.
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MR-96 Microplate Reader Operation Manual(2.0)
Jan.18,2005
Main Menu
18:18:18
Measure Program
Figure 3
The Main Menu displays the function keys and current date and time. You can enter the submenu
3.3. Measure
Touch the Measure button, the Select Test screen as shown in Figure 4 is displayed. Touch
Pgdn to display the tests of other pages circularly (42 tests in total, 3 pages). Touch the Exit
01 HBsAg 02 HBsAb
03 HBcAg 04 HBcAb
05 06
07 08
09 10
11 12
Pgdn Exit
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MR-96 Microplate Reader Operation Manual(2.0)
After you select the tests, the Plate No. and Vibrancy screen (Figure 5) is displayed.
Plate No. 00
Vibrancy
Exit OK
Touch Plate No. and enter the Plate No. in the Plate No. screen (Figure 6). As shown in figure 6, the
CLR button is to delete what you have entered. After entering, touch YES to confirm and return to
the Plate No. and Vibrancy screen. If you touch Exit , then the Plate No. is 00. Touch OK to begin
measuring and analyzing the ELISA plate. If you need to mix the sample by vibrating the plate before
1 2
3 4
5 6
7 8
9 0
. -
YES CLR
Exit
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MR-96 Microplate Reader Operation Manual(2.0)
3.4. Program
The Program screen includes Edit Test, New Test and Delete Test (Figure 7). Touch Exit to
Program
Edit Test
New Test
Delete Test
Exit
Touch Edit Test to select the test that you want to edit (Figure 4), then enter the Edit Test
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MR-96 Microplate Reader Operation Manual(2.0)
Plate Layout
Calculation Mode
Exit OK
Touch the Prim. Wav. button, a menu will then appear to ask you to select the wavelength.
NOTE: Don’t close all the filters; otherwise the alarm message “Lamp L” will appear.
Touch the Sec. Wav. button, a menu will then appear to ask you to select the wavelength.
In the Plate Layout screen (Figure 9-1), the right column is for selecting the well position
well columns. The box on top right corner displays the current strip being set. Touch
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MR-96 Microplate Reader Operation Manual(2.0)
C01 Sample 01
D01 Sample 02
E01 Sample 03
F01 Sample 04
H01 Sample 07
After selecting the well position, the screen as shown in figure 9-2 will be displayed. The left
column is for setting the filling type and filling No. of the selected well position (Sample: 1-96;
Standard: 1-10; Quality Control: 1-10; Neg./Pos. control: 1-12; Blank well: 1-12), use the
Pgdn button to view each No. of filling type. Touch the selected well position once more to
H01 Sample 07
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MR-96 Microplate Reader Operation Manual(2.0)
You can set the sample position quickly by using the Automatic button as shown in
figure 9-1. Since this function sets No. only for the original sample wells or blank wells, you
should touch Delete All (Figure 10) to delete all settings of the well positions before
auto filling. Then enter the Blank, Standard and Quality Control, etc, touch Automatic
and select Hor. Strip or Ver. Strip and No. of wells that you want to repeat.
Hor. Strip
Ver. Strip
Delete All
Exit
Touch the Calculation Mode button as shown in figure 8, then select a method in the right list
appeared.
2) Qual Calculation: You should set Qual Grouping, Cut-off, Neg. Range, Pos. Range, Validity
Confirm and so forth. Please make settings as instructed by the package insert.
a) Qual Grouping 1-4: You can divide the qualitative results into four groups (Negative-,
Positive+, Weak Positive-+ and Strong Positive ++) in accordance with the cut-off you
have set.
Where,
c) Neg. Range: When the absorbance exceeds the required range, the device will calculate
d) Pos. Range: When the absorbance exceeds the required range, the device will calculate
e) Validity Confirm 1-3: Set the range of absorbance for blank wells, positive and negative
control wells. The device will not calculate results when the absorbance exceeds the
range.
3) Quant Calculation: Some parameters are added on the basis of qualitative settings, such as
STD Concentration, Unit, Reference, Calibration Rule and Factor. Please make settings as
a) STD Concentration: You can set the concentrations of up to 8 standards. When setting,
you should begin with 1 from low concentration to high concentration. If No. of standards
Factor
A标
Formula: A =kC, K =
C标
This calibration rule requires only one standard to obtain the parameter k.
A2 − A1 A − A1
Formula: A =kC+b, K = , b = A1 − ( 2 )C1
C 2 − C1 C 2 − C1
This calibration rule requires two standards. C1 and C2 are concentrations of standard 1
Multipoint Linear
Formula: A =kC+b.
You can get values of k and b through the method of least squares.
Nonlinear
Draw a broken line passing through the standard concentration and its absorbance.
Measure the absorbance of the waiting samples and check relevant concentration on the
broken line.
Multipoint Percent
This calibration rule requires at least 3 standards as the multi-point broken line.
Exponent
Formula: A = k ln C + b
Logarithm
Formula: A = ke
bC
Power function
Formula: A = kC
b
f) Factor Setup: Enter the calibration parameters k and b which are mainly used in the factor
calculation method.
Touch the New Test button to select the test that you want to add or modify (refer to figure 4
for Select Test screen), and then enter the New Test screen (Figure 11).
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MR-96 Microplate Reader Operation Manual(2.0)
1ABCabc 2DEFdef
3GHIghi 4JKLjkl
5MNOmno 6PQRpqr
7STUstu 8VWXvwx
9Yzyz +-*/
0.,() NEXT
YES CLR
Exit
In the New Test screen, if you touch a button repeatedly, the screen will display the next
number, letter or symbol in circular way. NEXT is used to move the cursor
rightward to enter the next character. If you enter a wrong character, delete it with the
CLR button and enter again. Touch YES to save what you have entered and
Exit to return to the Program screen (Figure 7) without saving your entering.
Touch the Delete Test button, select the test that you want to delete (Figure 4).
Touch the Quality Control button, select the test that you want to view (Figure 4), then the QC
plot of this test will be displayed (Figure 12.). Touch the Settings button to set the QC parameters
of this test (V - Mean, SD - Standard Difference). If you want to print the QC data and QC plot, touch
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MR-96 Microplate Reader Operation Manual(2.0)
-2SD
-3SD
Print Exit
After touching the Delete Data button, you need to select Del. Last Month or Del. All Data, as
shown in figure 13. Touch Exit to return to the screen as shown in figure 12.
Exit
3.6. Setup
In the Setup screen (Figure 14), you can set the date and time, vibrant speed, light source, printer and
filter.
Setup
Filter
Exit
Touch the Date and Time button to set Year, Month, Day, Hour and Minute one by one. Touch YES
to confirm your settings and proceed to the next option until you finish all settings.
3.6.2. Vibrancy
The Vibrancy screen (Figure 15) includes Vibrant Speed and Vibrant Time. There are three speeds
available as High, Normal and Low. The vibrant time should not be longer than 60s.
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MR-96 Microplate Reader Operation Manual(2.0)
Exit
This button is used to turn on/off the lamp temporarily for the sake of the lamp’s lifespan.
NOTE: The lamp should be turned on and preheats for 10m before testing; otherwise the
3.6.4. Printer
This button is used to power on/off the printer, so you can select whether to print the results immediately
after testing.
3.6.5. Filter
The MR-96 reads the air blank absorbance of each filter in the 4 channels (F5 is spare), which is used to
check the photometric system. The default absorbance of the 405 - 630 filters is less than 0.500. However,
it may increase gradually in the result of dust, humidification and moldiness when the device is used for a
long time. If the absorbance is greater than 1.000, you should clean the photometric system or replace the
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MR-96 Microplate Reader Operation Manual(2.0)
3.7. Service
Data Transfer
Result Review
Standard Review
Touch the Data Transfer button to transport the test data to the computer which is connected to the
Touch the Result Review button and enter the No. of Plate that you want to search, then touch
YES to display the results. Touch the button to display circularly the results of column 0-6
and 7-12.
After touching the Standard Review button and selecting the test that you want to search, the screen
will display the standard absorbance and relevant concentration, as well as the calibration curve, as shown
in figure 16. However, if the selected test doesn’t use the Quant Calculation Mode, the results will not be
displayed).
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MR-96 Microplate Reader Operation Manual(2.0)
1 1.0 1.000
2 2.0 2.000
3 3.0 3.000
4 4.0 4.000
5 5.0 5.000
6 6.0 6.000
7 7.0 7.000
8 8.0 8.000
k 1.000
b 0.000
Exit
This function is used to read the absorbance of the waiting samples quickly. After touching the
Rapid Measure button, select the primary and secondary wavelength and enter the No. of samples.
Sec. Wav.
Vibrancy
Exit OK
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MR-96 Microplate Reader Operation Manual(2.0)
4. Maintenance
4.1. Notice
To ensure reliable performance and excellent operation of the MR-96, the operator must operate and
4.2. Maintenance
The MR-96 Microplate Reader is a precision optical measuring system. Therefore, it’s necessary to
1) Keeping the operating environment dry and clean. Covering the sample compartment after using
2) Keeping the mechanical and electrical parts of the device away from water and reagents.
3) Switching off the power supply while cleaning the surface and screen of the device. Be sure to use
5. Technical Specifications
2) Wavelength range: 405 nm, 450 nm, 492 nm, 630nm. 4 free positions for optional filters
8) Stability: ≤ 0.005A/10min
9) Reading Speed: Single wavelength <20s/plate (96-well plate); dual wavelength <35s/plate
(96-well plate).
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MR-96 Microplate Reader Operation Manual(2.0)
10) Vibrancy: Vibrant Speed (High, Normal, Low); Vibrant Time (0 - 60s).
6. Troubleshooting
The optical coupler is with dust Remove the dust and replace the
blocked
Sound is abnormal during Foreign matter exists Remove the foreign matter
local agency
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MR-96 Microplate Reader Operation Manual(2.0)
7. Warning
The MR-96 is a mini in vitro diagnosis instrument. It may bring risks to the patients indirectly, and
accompanying risks to the operator and environment. To avoid these risks, be sure to read the
This device adopts AC 220V which may bring electrical harm to human body. Don’t open
the rear cover before switching off the power supply. If you need to open the rear cover to maintain
For the patient samples may contain germina or virus which will cause disease, be
sure to dispose of the waste in accordance with relevant regulations of your hospital.
NOTE: Don’t operate the device unless you have read this Operation Manual carefully.
8. Packaging
Information indicated with symbols: FRAGILE, THIS WAY UP, KEEP AWAY FROM RAIN, DO
NOT ROLL.
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P/N: 0101-20-59654(2.0)