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I NSCA SPAIN NATIONAL CONFERENCE a decrease of BMD in postmenopausal compared with eumenor-
MADRID, SPAIN rheic women in well-trained females. The loss of BMD after men-
DECEMBER 13–14, 2019 opause seems to be not fully compensated by exercise (4), but this
ORAL ABSTRACT PRESENTATIONS could effectively mitigate the loss of BMD. Moreover, 1RM back
squat reported a slight association to BMD. Hence, strength train-
BONE MINERAL DENSITY IN
ing may be the best choice for preventing BMD loss. Health care
WELL-TRAINED FEMALES
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body fat; 48.52 6 4.41 ml$kg21$min21 V _ O2max) performed 3 displays very early in patients with CF [2]. Recent studies have
intervallic running sessions at different phases of the menstrual shown that CFTR protein is expressed in skeletal muscle, and
cycle (early-follicular phase: EFP; late-follicular phase: LFP; luteal therefore CF has a direct effect in this tissue [3]. Both respiratory
phase: LP). The protocol consisted of 8 intervals of 3 minutes at damage and muscular weakness result in a reduced exercise
85% of the maximal aerobic speed with 90-second rest between capacity that will lead to exercise intolerance and reduction of
them. Results: Mix linear model showed lower VE levels during expectoration and pulmonary function. Objective: Determine the
the LFP (74.25 6 12.78 L$min21), compared to the EFP (78.65 effect of a strength exercise program on muscle function in chil-
6 11.09 L$min21; p = 0.001) and the LP (78.50 6 13.40 dren with CF. Methods: Seventeen pediatric patients with CF
L$min21; p = 0.003). Similarly, V_ O2 reported a trend to significance participated in a randomized, prospective, controlled design. They
(p = 0.072) for menstrual cycle phase factor, being the lowest V _ O2 were divided in 2 groups: Control Group (CG) (n = 9; Age 12.43
registered during the LFP (2,148.50 6 346.01 ml$min21) com- 6 3.78 years; follow standard medical care), and Exercise Group
pared to the EFP (2,242.43 6 279.38 ml$min21) and the LP (EG) (n = 8; Age 12.75 6 3.06 years; follow 8 weeks of super-
(2,148.69 6 453.60 ml$min21). Discussion: Menstrual cycle vised exercise program). We measured muscle strength by 5RM
seems to influence VE response during an intervallic running pro- test of both the upper and lower limbs in pediatric weight training
_ O2. Specifically and contrary to litera-
tocol and to slightly affect V machines. Mean differences between groups and measurements
ture (2) both respiratory variables present their lower values during was determined using a mixed multivariate analysis of variance.
the LFP when the oestrogen peak is produced. This fact points out Results: The EG showed a significant improvement in strength
a higher respiratory efficiency for the same intensity and volume, relative to body weight compared to the CG (p = 0.015). A large
suggesting for coaches and athletes the LFP as the most suitable and significant effect size was observed on the leg press strength
for increments in respiratory strain. Nevertheless, more studies (p=0.003; h2p =0.514), bench press (p=0.008; h2p=0.428) and
analysing the LFP are needed to confirm our findings. Referen- seated lateral row (p=0.008; h2p =0.435). Discussion: In this
ces: 1. Janse de Jonge XA. Sport Med 33: 833–51, 2003. 2. preliminary data it seems that the muscles of the CF pediatric
Smekal G, et al. Med Sci Sport Exerc 39: 1098–106, 2007. patient can respond to exercise and improve muscle strength
Funding: The IronFEMME Study is supported by the Ministerio despite having CFTR deficit. Practical Applications: To maintain
de Economı́a y Competitividad, Convocatoria de ayudas I + D a correct body composition and physical condition in CF it’s
2016, Plan Estatal de Investigación Cientı́fica y Técnica y de In- related with better disease prognosis. Strength exercise programs
novación 2013–2016 (Contract DEP2016-75387-P). Mail to: may be a useful and capable tool to maintain and improve the body
laura.barba@upm.es. muscle mass in CF. References: 1. O’Sullivan et al. Cystic Fibro-
sis. Lancet 373: 1891–904, 2009. 2. De Meer et al. Peripheral
muscle weakness and exercise capacity in children with cystic
fibrosis. Am J Respir Crit Care Med 159: 748–54, 1999. 3.
EFFECT OF A STRENGTH EXERCISE PROGRAM Fiedler et al. Cloning and sequence analysis of rat cystic fibrosis
ON MUSCLE FUNCTION IN CHILDREN WITH transmembrane conductance regulator. Am J Physiol 262(6 Pt 1):
CF: PRELIMINARY RESULTS L779–84, 1992. Funding: This study was funded by Cátedra
Fundación Asisa-UE ref. 2018/UEM50. Mail to: f.cobovicente@
COBO F.,1,* SAN JUAN A.F.,2 FERNANDEZ-LUNA A.,1 gmail.com.
SANTANA E.,1 ITURRIAGA T.,1 DIEZ-VEGA I.,1
SANZ V.,3 AND PÉREZ- RUIZ M.1
1Universidad Europea de Madrid, Faculty of Sport Sciences,
Villaviciosa de Odo´n, Spain; 2Facultad Ciencias Actividad
THE INFLUENCE OF PRE-PREGNANCY
Fı´sica y Deporte-INEF, Universidad Polite´cnica Madrid, PHYSICAL ACTIVITY LEVELS ON CARDIAC
Madrid, Spain; and 3Hospital Universitario Nin˜o Jesu´s de FUNCTION DURING PREGNANCY
Madrid, Madrid, Spain
ROLDAN-REOYO O.,1 MCNARRY M.,1
Introduction: Cystic fibrosis is the most lethal autosomal reces- MACKINTOSH K.,1 GATZEMEIER J.,2 AND
sive disease with higher prevalence in Caucasian population [1]. LEWIS M.J.1
More than 1,800 mutations have been identified and can be clus- 1Collegeof Engineering, Swansea University; and 2College of
tered in 6 functional groups, therefore, altering the secretion and Health and Human Science, Swansea University
absorption in different tissues. Recurrent infections result in an
increased damage to the lungs that leads to hypoxia in peripheric Introduction: Pregnancy triggers some notable haemodynam-
tissues such as skeletal muscle. Then, skeletal muscle weakness ic adaptations that impose increased demands on the
Copyright © 2020 National Strength and Conditioning Association. Unauthorized reproduction of this article is prohibited.
Abstracts
cardiovascular system [1]. Although physical activity during COMPARISON OF MAXIMAL AEROBIC SPEED
pregnancy does not impair resting cardiac function [2], its OBTAINED IN A TREADMILL OR A TRACK TEST
influence during acute exercise is more poorly understood. IN MIDDLE-DISTANCE WOMEN RUNNERS
Pre-pregnancy physical activity level (PAL) could also poten-
tially modify the cardiac response to acute exercise during the MARCO-CONTRERAS L.A.,1,2 BACHERO-MENA B.,3
subsequent antenatal period. This study therefore sought to AND Y GONZÁLEZ-BADILLO J.J.1
determine whether pre-pregnancy PAL influences the antenatal 1Universidad Pablo de Olavide de Sevilla, Espan˜a;
cardiac response to acute exercise, assessed via heart rate 2Universidad San Jorge de Zaragoza, Espan˜a; and
(HR) and stroke volume (SV). Methods: Twenty-six pregnant 3Universidad de Sevilla, Espan˜a
the TM
Copyright © 2020 National Strength and Conditioning Association. Unauthorized reproduction of this article is prohibited.
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16: 456–64, 2014. 4. Jones AM, et al. J Sports Sci 14: 321–7, of metabolic stress (5). Contrary to findings observed in patholog-
1996. 5. Pugh LG. J Physiol 207: 823–35, 1970. Mail to: ical and sedentary subjects under N conditions (1,6) miR-378 does
luisalbertomarcocontreras@gmail.com. not seem to influence the immediate adaptations to a hypertrophy-
RT protocol in trained subjects. Our findings do not support
a potentiated effect of acute moderate terrestrial hypoxia on meta-
bolic and hormonal factors linked to muscle growth after a hyper-
ACUTE MODERATE ALTITUDE EFFECT ON trophic resistance training session. Fundings: DEP2015-64350-
METABOLIC RESPONSE AFTER P, MINECO/FEDER and PGC2018-097388-B-I00. References:
A HYPERTROPHY-ORIENTED RESISTANCE 1. Davidsen, et al. J Appl Physiol 110:309–317, 2011. 2. Millet,
TRAINING SESSION et al. J Appl Physiol 112: 1783–1784, 2012. 3. Schoenfeld, BJ.
Sport Med 43: 179–194, 2013. 4. Scott, et al. Sport Med 44:
BENAVENTE C., FERICHE B., SHOENFELD B.J., 1037–1054, 2014. 5. Scott, et al. J Strength Cond Res 31:
BONITCH-GÓNGORA J., DE LA FUENTE B., 1973–1981, 2017. 6. Xu, et al. Oncotarget 7: 12414–12425,
ALMEIDA F., ORTEGA-RODRÍGUEZ R., AND 2016. Mail to: crismibb@hotmail.com.
PADIAL P.
1Department of Physical Education and Sport, Faculty of
Sport Sciences, University of Granada, Spain; 2Department of ECCENTRIC OVERLOAD TRAINING IN YOUTH
Health Sciences, CUNY Lehman College, Bronx, NY; and
3High performance Center of Sierra Nevada, Spanish Sport
TEAM FEMALE ATHLETES: TRAINING
CONDITIONS AND RELATIONSHIP WITH
Council, Spain
PERFORMANCE CHANGES
Introduction: Evidence supports a potential benefit of resistance
ALGIRDAS STUKNYS, JORGE AREDE, MARC
training in a hypoxic environment for enhancing the hypertrophic
MADRUGA, VICTOR LOPEZ-ROS, OLIVER
response, possibly due to an increased production of metabolites
GONZALO-SKOK, AND NUNO LEITE
linked to muscle growth mechanisms (3,4). However, studies to
date have exclusively employed simulated hypoxia while it has been Lithuanian Sports University, Kaunas, Lithuania; Research
suggested that natural altitude is a more severe environmental con- Center in Sports Sciences, Health Sciences and Human
Development, CIDESD, Vila Real, Portugal; University
dition (2). In addition, some microRNAs, which could be significant
School of Health and Sport (EUSES), University of Rovira
regulators of muscle protein expression and local factors, are being
Virgili, Amposta, Spain; University School of Health and Sport
currently studied in regard to gains in strength and muscle mass
(EUSES), University of Girona, Girona, Spain; Faculty of
(1). This study investigated the effect of acute exposure to moder- Education and Psychology, University of Girona, Girona,
ate altitude on the metabolic stress and associated responses Spain; and Faculty of Health Sciences, University of San Jorge,
induced by a standard hypertrophy-oriented RT session. Method: Zaragoza, Spain
Thirteen males completed 2 counterbalanced hypertrophic RT ses-
sions (3 set 3 10RM, 2 minutes rest) under moderate altitude Introduction: Eccentric overload training is a useful method for
(H;2320 m asl) and normoxic conditions (N; ,700 m asl) with the inter-limb asymmetries detection in sport-specific actions (1)
72 h rest between sessions. Blood (lactate [Lac], inorganic phos- and beneficial to improve unilateral lower limb strength power (2).
phate [Pi], calcium [Ca2], liquid carbon dioxide [CO2L]), hormones We aim to analyze the relationship between training variables
(testosterone [T] and growth hormone [GH]) and miRNA (miR- (velocity, power and movement variability) and mid-term changes
378) were assessed before and throughout 30 minutes post- in single leg countermovement jump (SLCMJ), according training
exercise. Results: Compared to pre-exercise, RT increased serum conditions. Methods: With written informed consent obtained, and
levels of Ca2+, GH and Lac (p0.05) under H and N conditions. conformed to the recommendations of the Declaration of Helsinki,
Altitude effects were observed in Pi and CO2L (3.00 6 0.71 vs 13 under-16 female basketball and volleyball players performed
3.41 6 1.03 mg$dl21; p = 0.044 and 21.06 6 2.62 vs 25.35 6 a 6-week EOT using a flywheel device (Eccomii, Byomedic) which
2.30 mmol$L21; p = 0.020 respectively for H and N). Serum consisted of 1 specific change of direction exercise (Shuffle Step
miRNA-378 and T remained unchanged at all studied conditions [SS] in right and left directions, 1 set of 6 reps, twice/week). The
(p . 0.05). Discussion: The acute moderate altitude did not affect constant group (n = 7) performed the SS in one direction (e.g.,
the serum level of ions, hormones nor miR-378 measured after right) and, thereafter, in the other direction, while variable group (n =
a RT session. Despite the strong influence of oxygen availability 6) performed the exercise in 3 random directions (12 reps in total).
on phosphocreatine resynthesis, recovery times longer than 90s During training sessions, acceleration was measured using an iner-
could limit hypoxic effects and reduce the potential anabolic impact tial measurement unit (WIMU, Realtrack Systems), and the mean
Copyright © 2020 National Strength and Conditioning Association. Unauthorized reproduction of this article is prohibited.
Abstracts
velocity and power of both concentric (CON) and eccentric (ECC) measured using electronic photocells (placed at 0, 10, 20 and
phases were collected using Chronojump software (v1.9). Sample 30 m) and the step frequency and step length were determined
Entropy (SampEn) were computed using SPRO Software v1.0.0 using a video analysis method. RSA was assessed using 5 scores
(Realtrack Systems). The SLCMJs (horizontal, vertical, and lateral) for sprint time, step frequency and step length: the best, the mean
were assessed before and after the training protocol. Results: In and the worst of the 6 repetitions, and 2 fatigue indexes [Fmean =
constant group, the CON velocity (r = 0.80) and power (r = 0.81) (mean/best 3 100) 2 100; Fworst = (worst/best 3 100) 2 100].
recorded during SS left training were significantly correlated with Results: Sprint times increased from the first to the last repetition
changes in SLCMJ right (lateral). In variable group, the CON veloc- (4.1%; p , 0.001), whereas step frequency decreased across
ity (r = 20.95) and power (r = 20.89) during SS left were signif- trials (4.0%; p , 0.001) and step length remained constant. Sprint
icantly correlated with changes in SLCMJ left (lateral). Also, the times decreased (p , 0.001) and step length increased (p ,
SampEn (r = 20.94) during SS left were significantly correlated 0.001) across the 10-m sections into which the sprint was divided,
with changes in SLCMJ left (vertical). Furthermore, the CON veloc- whereas step frequency showed greater values in the 10–20 m
ity (r = 20.94) during SS right were significantly correlated with section in relation to 0–10 m (p , 0.01) and 20–30 m (p , 0.001)
changes in SLCMJ left (lateral). Discussion: Training variables zones. Very large correlations were observed between frequency
during EOT and mid-term changes in SLCMJ are differently related and sprint time for both fatigue indexes (Fmean: r = 20.81, p ,
according to training condition. Practitioners could provide more 0.001; and Fworst index: r = 20.77, p , 0.01), but not for step
accurate daily basis real-time feedback to achieve expected results length. Discussion: Our results suggest that changes in sprint
for each training condition. References: 1. Madruga-Parera, M, times could be related to variations in step frequency during the
Bishop, C, Fort-Vanmeerhaeghe, A, Beltran-Valls, MR, Gonzalo- repeated sprints. This might suggest that maintaining stride fre-
Skok, O, and Romero-Rodriguez, D. Interlimb asymmetries in youth quency values during speed training might be a critical aspect for
tennis players: relationships with performance. J Strength Cond performance in repeated sprint ability tests. References: 1. Ram-
Res 2019. 2. Gonzalo-Skok O, Moreno-Azze A, Arjol-Serrano JL, pinini E, Bishop D, Marcora SM, Ferrari Bravo D, Sassi R, Im-
Tous-Fajardo J, Bishop C. A comparison of 3 different unilateral pellizzeri FM. Validity of simple field test as indicators of match-
strength training strategies to enhance jumping performance and related physical performance in top-level professional soccer play-
decrease inter-limb asymmetries in soccer players. Int J Sport Phys- ers. J Sports Med 28: 228–235, 2007. 2. Girard O, Brocherie F,
iol Perform Perform 2019. Mail to: algirdasstuknys@gmail.com. Morin JB, et al. Comparison of 4 sections for analysing running
mechanics alterations during repeated treadmill sprints. J Appl Bio-
mech 31: 389–95, 2015. Mail to: p.gfrutos.prof@ufv.es.
Copyright © 2020 National Strength and Conditioning Association. Unauthorized reproduction of this article is prohibited.
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Eighty-one young adults (51 males; mean age, 22.7 6 4.4 years Introduction: Intense running exercise produces IL-6 eleva-
old) were included. An incremental exercise protocol in cycle tion, as either myokine, inflammatory cytokine (2) or both. It
ergometer with 2 consecutive phases was performed to deter- results in the up-regulation of hepcidin 3 hours afterwards (2),
mine MFO and V _ O2max using indirect calorimetry. The first phase reducing iron availability (3). This well-known process could
aimed to determine MFO and consist in 3 minutes steps of 15/30 be affected by oestrogen and progesterone fluctuations dur-
W (depending on weight status) increments with a cadence of ing the menstrual cycle, since both hormones had been re-
60–80 rpm. The test was stopped when RER $1. After 3– ported as hepcidin modulators (3). The purpose of this study
5 minutes rest, the second phase to detect the V _ O2max was ini- was to compare the influence of menstrual cycle phases on
tiated with steps of 1 minute and same incremental load until hepcidin and IL-6 regulation after an intervallic running pro-
exhaustion. Bioimpedance analysis was used to assess body tocol in women. Methods: Twenty-one endurance-trained
composition (fat mass and fat free mass (FFM)), following the females (31.75 6 5.69 years; 57.63 6 8.63 kg; 162.96 6
corresponding previous considerations. The muscular strength 6.51 cm; 25.23 6 6.73% body fat; 48.52 6 4.41
(MS) was assessed by handgrip dynamometry and distance in ml$min21$kg21 V_ O2max) performed an intervallic running pro-
longitudinal jump test. Linear regression analyses were performed tocol in the early-follicular phase (EFP), late-follicular phase
to determine the independent associations of CRF and MS on fat (LFP) and mid-luteal phase (LP). These sessions consisted of
oxidation (MFO and MFO/FFM) and sex interaction. ANCOVA 8 3 3 minutes intervals at 85% V_ O2max speed with 90-
was used to observe differences between CRF and MS on seconds recoveries. Blood samples were collected pre-
MFO using combined groups separated by high/low CRF and exercise, 0, 3 and 24 h post-exercise. Results: Mix linear
high/low MS. Then, the differences in MFO between groups were model showed higher hepcidin concentrations at 3 h post-
studied by analysis of variance. Results: MS/FFM and CRF were exercise during the LFP (3.01 6 4.16 nM$L21) than in the
positively correlated with MFO/FFM (r = 0.23, p = 0.04; r = 0.57, EFP (1.26 6 1.25 nM$L21; p = 0.003) and MLP (1.75 6 1.98
p , 0.001). No interaction between sex was found. Significative nM$L21; p = 0.052). As expected, hepcidin exhibited higher
differences were observed between HighCRF/HighMS and values at 3 h post-exercise (2.01 6 2.81 nM$L21) in compar-
LowCRF/LowMS (p , 0.001), HighCRF/HighMS and ison with pre-exercise (0.93 6 1.71 nM$L21; p , 0.001), 0 h
LowCRF/HighMS (p = 0.004). Discussion: Maximal fat oxidation (1.13 6 1.43 nM$L21; p = 0.001) and 24 h post-exercise
capacity is highly influenced by CRF, but the role of MS assessed (1.36 6 2.25 nM$L21; p = 0.026). According to the literature,
by handgrip and longitudinal jump is not relevant. References: 1. IL-6 was higher at 0 h post-exercise (4.44 6 2.26 pg$ml21)
Achten J, Jeukendrup A. Optimizing fat oxidation through exercise compared with pre-exercise (1.70 6 0.71 pg$ml21; p ,
and diet. Nutrition 20: 716–727, 2004. 2. Venables M, Achten J, 0.001), 3 h (1.71 6 0.56 pg$ml21; p , 0.001) and 24 h
Jeukendrup A. Determinants of fat oxidation during exercise in post-exercise (1.81 6 1.06 pg$ml21; p , 0.001). Lastly, no
healthy men and women: A cross-sectional study. J Appl Physiol differences were found regarding IL-6 and menstrual cycle
98: 160–167, 2005. Mail to: ed.opazodiaz@alum.uca.es. phase. Discussion: Menstrual cycle affects hepcidin
POSTER ABSTRACT PRESENTATIONS response to running exercise, but not IL-6. This fact points
out the likely influence of sex hormones on hepcidin synthesis
(3). LFP seems to be the less suitable period of the menstrual
cycle to restore iron reserves in active women, since iron
HEPCIDIN REGULATION IN PHYSICALLY absorption and recycling are expected to be lower than in
ACTIVE WOMEN AFTER AN INTERVALLIC other menstrual cycle phases. Therefore, iron fortified diets
RUNNING PROTOCOL THROUGHOUT THE or supplementation should preferably be reinforced in EFP
MENSTRUAL CYCLE and MLP when the goal is to restore iron reserves. Referen-
ces: 1. Hojman P, et al. Am J Physiol Metab 316: E940–7,
ALFARO-MAGALLANES V.M.,1 RAEL B.,1
2019. 2. Peeling P, et al. Eur J Appl Physiol 103: 381–91,
ROMERO-PARRA N.,1 BARBA-MORENO L.,1
2008. 3. Sangkhae V, Nemeth E. Adv Nutr 8: 126–36, 2017.
BODOQUE M.,1 CUPEIRO R.,1 BENITO P.J.,1 AND
Funding: The IronFEMME Project is funded by the Ministerio
PEINADO A.B.,1 ON BEHALF OF THE IRONFEMME de Economı́a y Competitividad, Convocatoria de ayudas I + D
STUDY GROUP 2016, Plan Estatal de Investigación Cientı́fica y Técnica y de
1LFE Research Group, Department of Health and Human Innovación 2013–2016 (Contract DEP2016-75387-P). Mail
Performance, Faculty of Physical Activity and Sport Science to: vm.alfaro@upm.es.
(INEF), Universidad Polite´cnica de Madrid
Copyright © 2020 National Strength and Conditioning Association. Unauthorized reproduction of this article is prohibited.
Abstracts
EFFECTS OF THE 8-WEEK RESISTANCE Del Coso J, et al. Scand J Med Sci Sport. 26: 156–64, 2016.
TRAINING PROGRAMME WITH ELASTIC BANDS Mail to: angelc.rayo@gmail.com.
FOR PEOPLE WITH MULTIPLE SCLEROSIS
Copyright © 2020 National Strength and Conditioning Association. Unauthorized reproduction of this article is prohibited.
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JM, et al. J Appl Physiol 122: 559–70, 2017. 2. Douglas J, et al. 0.08; ES = 0.94), and concentric CMJ phases (+0.04 seconds,
Sport Med 47: 663–75, 2016. 3. Fernandez-Gonzalo R, et al. J p = 0.17; ES = 0.56). Discussion: In this preliminary data in
Sport Sci Med 10: 692–9, 2011. Funding: The IronFEMME Study Olympic-level boxers it seems that CAFF improves CMJ height.
takes place with the financial support of the Ministerio de Economı́a Our results agree with those of previous studies in Jiu-jitsu ath-
y Competitividad, Convocatoria de ayudas I + D 2016, Plan Estatal letes [4]. Further, the analysis of CMJ eccentric and concentric
de Investigación Cientı́fica y Técnica y de Innovación 2013–2016 phases suggest an enhancement of the stretch-shortening cycle.
(Contract DEP2016-75387-P). Mail to: n.romero@upm.es. These effects of CAFF could be mediated by enhanced motor
neuron transmission and by increased activity of the sodium-
potassium pump [5]. Practical Applications: It seems that
EFFECTS OF CAFFEINE SUPPLEMENTATION ON CAFF at an ergogenic dose (6 mg$kg21) would improve lower
COUNTERMOVEMENT JUMP PERFORMANCE IN limb muscular power in high-performance boxers. References: 1.
OLYMPIC-LEVEL BOXERS: Markovic et al. Reliability and factorial validity of squat and coun-
PRELIMINARY RESULTS termovement jump tests. J Strength Cond Res 18: 551–555,
2004. 2. Glaister et al. Caffeine and physiological responses to
SAN JUAN A.F.,1 LAGO-RODRÍGUEZ A.,2 LÓPEZ- submaximal exercise: A meta-analysis. Int J Sports Physiol Per-
SAMANES A.,3 VEIGA-HERREROS P.,4 SÁNCHEZ- form 13: 402–411, 2018. 3. López-González et al. Acute caffeine
OLIVER A.J.,5 PÉREZ-LÓPEZ A.,6 JODRA P.,4,7 supplementation in combat sports: A systematic review. J Int Soc
AND DOMÍNGUEZ R.2 Sports Nutr 15: 60, 2018. 4. Diaz-Lara et al. Caffeine improves
1Facultad muscular performance in elite Brazilian Jiu-jitsu athletes. Eur J
Ciencias Actividad Fı´sica y Deporte-INEF,
Sport Sci 16: 1079–1086, 2016. 5. Bishop D. Dietary supple-
Universidad Polite´cnica Madrid, Madrid, Spain; 2Facultad de
Ciencias de la Salud, Universidad Isabel I, Burgos, Spain; ments and team-sport performance. Sports Med 40: 995–1017,
3Facultad de Ciencias de la Salud, Universidad Francisco de 2010. Funding: This research was funded by Fundación Univer-
Vitoria, Pozuelo, Spain; 4Facultad de Ciencias de la Salud, sidad Alfonso X el Sabio and Banco Santander. Mail to:
Universidad Alfonso X el Sabio, Villanueva de la Can˜ada, alejandro.sanjuan@upm.es.
Spain; 5Facultad de Ciencias de la Actividad Fı´sica y del
Deporte, Universidad de Sevilla, Sevilla, Spain; 6Facultad de
Medicina y Ciencias de la Salud, Universidad de Alcala´, Alcala´
de Henares, Spain; and 7Departamento de Ciencias de la IS MTDNA COPY NUMBER A GOOD
Educacio´n, Universidad de Alcala´, Alcala´ de Henares, Spain BIOMARKER FOR PREDICT
CARDIORESPITATORY FITNESS LEVEL?
Introduction: Vertical jump measured through countermove-
ment jump, is an indicator of lower limbs muscular power [1]. SÁNCHEZ-LORENTE I.M.,1 ITURRIAGA T.,1
Caffeine (CAFF) supplementation has been considered an ergo- GARCÍA-MERINO J.A.,3 SÁNCHEZ-BARROSO L.,1
genic aid in endurance sport modalities [2]. Moreover, since FERNÁNDEZ-ELÍAS V.E.,2 PÉREZ M.,2
CAFF increases motor unit recruitment and muscular contraction, SANTIAGO C.,2 AND YVERT T.2
it has been also proposed as an ergogenic aid in combat modal- 1Facultad de Ciencias de la Actividad Fı´sica, el deporte y
ities [3]. Objective: Determine the effects of caffeine supplemen-
fisioterapia, Universidad Europea de Madrid, Madrid, Spain;
tation on CMJ performance in Olympic-level boxers. Methods: 2Facultad de Ciencias Biome´dicas y de la Salud, Universidad
Five healthy male athletes, members of the Spanish Boxing Europea de Madrid, Madrid, Spain; and 3Facultad de
National Olympic Team (age: 22.4 6 1.94 years, height: 1.71 Enfermerı´a, Fisioterapia y Podologı´a, Universidad
6 0.08 m, body-mass: 67.52 6 10.45 kg, body mass index: Complutense de Madrid, Madrid, Spain
22.74 6 1.35 kg$m22), participated in the study. In a randomized
double-blind, placebo-controlled, counterbalanced, crossover Introduction: Mitochondrion is the cellular organelle that produ-
design, the athletes completed 2 identical assessment sessions ces energy for the organism, generating adenosine triphosphate
(i.e., CMJ performance on a force platform) at the same time slot using O2 and nutrients. Physical capacity levels are thus directly
(60.5 hours) after the intake of CAFF (6 mg$kg21) or placebo. related with mitochondrial activity (1). Mitochondrial DNA (mtDNA)
Mean differences between measurements was determined using copy number is proportional to the mtDNA volume in a cell, and
a paired samples t-test. Pairwise comparisons significance was can be used as a measure of mitochondrial function (2). The aim of
assessed by calculating Hedges’ g Effect Size (ES). Results: this study was to evaluate the possible relationship between car-
CAFF improved jump height (+3.1 cm, p = 0.02; ES = 0.76), diorespiratory fitness level and leukocyte relative mtDNA copy
and enhanced the time of the eccentric (20.13 seconds, p = number in healthy men. Methods: One hundred three healthy
Copyright © 2020 National Strength and Conditioning Association. Unauthorized reproduction of this article is prohibited.
Abstracts
men aged between 18 and 45 divided into 2 groups: high cardio- Introduction: High soluble-fraction levels of Alpha-Klotho
respiratory fitness level with V_ O2max above 55 ml$kg21$min21 have been related with anti-inflammatory effects on the organ-
(group 1) and low cardiorespiratory fitness level with V _ O2max ism, decreased degenerative processes, and longevity [1–2].
21 21 _
below 45 ml$kg $min (group 2). VO2max was measured in In addition, SaKl levels increase immediately after an acute
a maximal incremental treadmill test a with gas analyser (ULTIMA endurance exercise that does not occur after plyometric exer-
series, MEDGRAPHICS, cardiorespiratory diagnostic). mtDNA cise [3]. Nevertheless, there is no literature to date regarding
copy number was measured in peripheral blood leukocytes. Blood the behavior of the protein during the recovery period follow-
samples were collected in collection tubes containing EDTA and ing plyometric exercise. Due to its well-known anti-
DNA extraction was obtained using the High Pure PCR Template inflammatory response, we hypothesized that a SaKl levels
Preparation Kit. The leukocyte relative mtDNA copy number was increment could happen during the hours following a plyomet-
analysed using a real-time polymerase chain reaction (RT-PCR) ric exercise session. Our main objective was to analyze the
method with SYBR Green. Threshold cycle number (Ct) values acute effect of a single plyometric exercise bout on SaKl and
of the b-globin nuclear gene and the COX1 mitochondrial gene its kinetics during the 72 following hours. Method: Thirty-
were used to determine the mtDNA content. The content of the nine physically-active males (aged = 18–35; V_ O2max =
mtDNA was calculated using the following equation: relative copy 55.6 6 5.19 ml$kg21$min21) underwent one eccentric-
number = 2DCt (DCt = Ct nDNA 2 Ct mitDNA). For statistical focused plyometric session. Their serum SaKL and
analyses, we used the U the Mann-Whitney test with software Creatine-Phosphokinase (CPK) levels were measured before
SPSS for Windows version 25.0 (SPSS Inc., Chicago, IL, USA). (PRE) and immediately after (POST) the session, as well as
Results: V _ O2max was 58.7 6 3.9 ml$kg21$min21 for group 1 and 24, 48 and 72 hours later. Results: We found overall signif-
39.6 6 3.8 ml$kg21$min21 for group 2. We found significant icant differences in the SaKl kinetics (F = 4.182; p = 0.011).
differences in mtDNA copy number between the groups SaKl increased significantly between POST and 24 h (p =
(392.18 6 881.2 for group 1 and 156.41 6 154.3 for group 2) 0.041; d = 0.499) and between POST and 48 h (p = 0.007;
(p = 0.044). Discussion: Our results are in accorance to Chang d = 0.873). Statistically significant differences were also
et al. (2016) results in postmenopausal women (3) and to Bayarca observed in the CPK measures through the different time
et al. (2016) results in athletes (4). In conclusion, it seems that intervals (F = 22.591; p = 0.001). Discussion: There are
mtDNA copy number can represent an interesting novel biomarker some studies focusing on the SaKl response to aerobic or
for cardiorespiratory fitness capacity level. Practical implementa- anaerobic exercise [1, 4], but to the best of our knowledge
tion: mtDNA copy number is a good indirect predictor of mitochon- this is the first study analyzing the acute effect of a specific
drial function and usefull for mesure mitochondrial biogenesis in plyometric exercise protocol on SaKl secretion during 72 h.
physical activity. References: 1. McBride et al. Curr Biol 16: SaKl levels presented a late response after the exercise ses-
R551–60, 2006. 2. Eynon et al. Physiological Genomics 43: sion, remaining elevated from 24 to 72 h post exercise. Fur-
789–798, 2011. 3. Chang et al. Korean J Fam Med 37: 334– thermore, SaKl followed similar kinetics to CPK, a muscle
339, 2016. 4. Baykara et al. Cell Mol Biol 62: 109–115, 2016. damage and inflammation biomarker. In conclusion, we sug-
Funding: Universidad Europea de Madrid. Ref: 2018UEM05. gest that Klotho protein could play an anti-inflammatory role
Mail to: isasanchezlorente@gmail.com. after plyometric exercise. And although more studies are
needed, in this regard, the finding could have a relevant prac-
tical implication both in the clinic and in sport. References:
1. Saghiv MS et al. The effects of aerobic and anaerobic
KINETICS OF Sa-KLOTHO PROTEIN AFTER exercises on circulating soluble-Klotho and IGF-I in young
PLYOMETRIC EXERCISE IN PHYSICALLY and elderly adults and in CAD patients. J Circ biomarkers
ACTIVE YOUNG MEN 6: 1849454417733388, 2017. 2. Semba RD et al. Plasma
Klotho and Cardiovascular Disease in Adults. J Am Geriatr
ITURRIAGA T.1, YVERT T.,2 SANCHEZ-LORENTE Soc 59: 1596–601, 2011. 3. Saghiv M et al. Comparison
I.M.,1 DIEZ-VEGA I.,1 FERNANDEZ-ELIAS V.M.,1 between aerobic and anaerobic training influence on s-
SANCHEZ-BARROSO M.,2 PEREZ-RUIZ M.,2 AND Klotho blood levels following 60 minutes aerobic bout. JJ
SANTIAGO C.2 Physiol 1: 4, 2015. 4. Mostafidi E et al. Serum Klotho Levels
1Facultyof Physical Activity, Sport Science and physiotherapy, in Trained Athletes. Nephrourol Mon 8: e30245–e30245,
Universidad Europea de Madrid, Spain; and 2Faculty of 2016. Funding: Universidad Europea de Madrid (Ref:2019/
Biomedical and Health Sciences, Universidad Europea de UEM08). Mail to: tamara.iturriaga@universidadeuropea.es.
Madrid, Spain
the TM
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30 M-SPRINT DIFFERENCES IN PROFESSIONAL tice. PeerJ 6: e5937, 2018. Mail to: cortesarmadaestrella@
FOOTBALL PLAYERS WITH AND WITHOUT AN gmail.com.
INJURY HISTORY: PRELIMINARY RESULTS
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Abstracts
Maggi S. Sarcopenia and fragility fractures. Eur J Phys Rehabil 1. Behm D, et al. Sport Med 45: 1645–69, 2015. 2. Kohler J,
Med 49: 111–117, 2013. 2. Narici MV, Maffulli N. Sarcopenia: et al. J Strength Cond Res 24: 313–21, 2010. 3. Saeterbakk-
Characteristics, mechanisms and functional significance. Br en A, et al. Eur J Appl Physiol 116: 2247–56, 2016. 4. Górski
Med Bull 95: 139–159, 2010. 3. Granacher U, Gollhofer A, M, et al. J Hum Kinet 63: 95–103, 2018. 5. Lawrence M, et al.
Hortobágyi T, Kressig RW, Muehlbauer T. The importance of J Strength Cond Res 00, 2018. Mail to: moises.mnieto@
trunk muscle strength for balance, functional performance, and alumnos.upm.es.
fall prevention in seniors: A systematic review. Sport Med 43:
627–641, 2013. 4. Fernández Roldán K, Benı́tez Jiménez A.
Influence of the pilates mat practice on the sarcopenia. KRO-
NOS Act fı´sica y salud 1: 51–55, 2013. 5. Fiatarone MA. High- DIFFERENT FACTORS AFFECT MAXIMAL FAT
Intensity Strength Training in Nonagenarians. JAMA 263: OXIDATION DURING EXERCISE IN HEALTHY
3029, 1990. Mail to: ruben.fernandez12@alu.uclm.es. ADULTS: A CROSS-SECTIONAL STUDY
VELÁZQUEZ-DÍAZ D.,1,2 PÉREZ-BEY A.,2,3
CORRAL-ROMAN J.,1,2 FERNÁNDEZ-SANTOS J.R.,2,3
EFFECT OF INSTABILITY ON POWER AND MARÍN-GALINDO A.,2 MONTES-DE-OCA A.,2
EXECUTION SPEED IN PUSH-UP JIMÉNEZ-PAVÓN D.,1,2 AND
PONCE-GONZÁLEZ J.G.1,2
MARQUINA M.,1 RIVILLA J.,1 LORENZO J.,1 AND
1MOVE-IT Research group and Department of Physical
DE LA RUBIA A.1
Education, Faculty of Education Sciences, University of Ca´diz,
1Facultad de Ciencias de la Actividad Fı´sica y el Deporte, Ca´diz, Spain; 2Biomedical Research and Innovation Institute
Universidad Polite´cnica de Madrid of Ca´diz (INiBICA) Research Unit, Puerta del Mar
University Hospital University of Ca´diz, Spain; and
Introduction: Strength training using devices that induce dif- 3GALENO Research group, University of Ca´diz, Ca´diz, Spain
Copyright © 2020 National Strength and Conditioning Association. Unauthorized reproduction of this article is prohibited.
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which affect fat oxidation (b ranges from 20.2 to 1.02; p groups (p , 0.001). Significant differences were observed
0.001, respectively), however this positive association with between the group ReWin(d) and group placebo at 48 h
MS disappeared when CRF and MS were introduced in the post-exercise (p = 0.004; d = 0.82). The muscular power of
same regression model, and on the other hand this association the lower limb showed significant interaction between the
was maintained to CRF. Conclusions: More than 50% of ReWin(d) and placebo groups in the first 24 hours (p =
MFO could be explained by individual fat mass, dietary intake, 0.049; d = 0.63). The muscular power in the placebo group
TAG, OPA, MS and CRF. However, the CRF was the main moderately decreased in the first 24 h. Discussion: Results
factor that affects fat oxidation. References: 1. Venables show that the ReWin(d) supplement in trained youth athletes
MC, Achten J, Jeukendrup AE. Determinants of fat oxidation attenuates DOMS achieving an anticipated recovery compared
during exercise in healthy men and women: a cross-sectional to the placebo group. Practical Applications: Although more
study. J Appl Physiol 98: 160–7, 2005. Mail to: daniel. studies to corroborate these initial results are needed, it seems
velazquez@uca.es. that with the ReWin(d) supplement could anticipate the next
bout of exercise performed by athletes. References: 1. Sell-
ami M, Gasmi M, Denham J, et al. Effects of Acute and Chronic
Exercise on Immunological Parameters in the Elderly Aged:
EFFECT OF THE HERBAL SUPPLEMENT Can Physical Activity Counteract the Effects of Aging? Front
REWIN(D) ON THE RECOVERY OF DELAYED Immunol 9: 2187, 2018. 2. Haefeli WE. Individualisierung der
ONSET MUSCLE SORENESS INDUCED- evidenz—Single case randomized trial. Ther Umschau 63:
EXERCISE 777–81, 2006. 3. Howatson G, Hoad M, Goodall S, et al.
Exercise-induced muscle damage is reduced in resistance-
DOMÍNGUEZ-BALMASEDA D.,1 DIEZ-VEGA I.,2 trained males by branched chain amino acids: A randomized,
LARROSA M.,1 ISSALY N.,3 BURGOS S.,2 SAN JUAN double-blind, placebo controlled study. J Int Soc Sports Nutr 9:
A.F.,4 ROLLER M.,6 AND PÉREZ-RUIZ M.2 20, 2012. Funding: This work was supported by Natural Ori-
1Faculty of Biomedical Sciences, Universidad Europea de gin grant and conducted in the Universidad Europea de Ma-
Madrid, Madrid, Spain; 2Faculty of Sport Sciences, drid, Spain. Mail to: diego.dominguez@universidadeuropea.
Universidad Europea de Madrid, Madrid, Spain; 3Nicolas es.
Issaly, Valencia, Spain; 4Faculty Sciences Physical Activity and
Sport-INEF, Universidad Polite´cnica Madrid, Madrid,
Spain; and 5Natural Origins, Lozanne, France
RATE OF PERCEIVED EXERTION (RPE)
Introduction: The need for strength training with a large com- METHOD FOR QUANTIFYING TRAINING LOADS
ponent of eccentric contraction to achieve positive muscle DURING AN ECCENTRIC- OVERLOAD
adaptations is inherent to the development of delayed onset SQUAT EXERCISE
muscle soreness. Herbal supplementation has increased
among athletes, in order to increase muscle hypertrophy and SOSA-GONZÁLEZ A.,1 DOS’SANTOS T.,2 BRUGAT-
beta-oxidation of fats to improve performance (1). The objec- TRUJILLO P.,1 BELTRAN-GARRIDO V.,3 ROMERO-
tive was to investigate the effectiveness of a polyherbal mixture RODRIGUEZ D.,1 AND MADRUGA-PARERA M.1,3
in the muscle pain and performance in young athletes after 1University School of Health and Sport (EUSES), University
undergoing inducing eccentric exercise. Methods: The study
of Girona, Girona, Spain; 2Human Performance Laboratory,
followed a double-blind, placebo-controlled design (n = 40)
Directorate of Sport, Exercise, and Physiotherapy, University
carried out in physically active males (18–35 years), randomly of Salford, Greater Manchester, United Kingdom; and
assigned to ReWin(d) 2 g intake (natural organic blend of 3University School of Health and Sport (EUSES), University
a Zingiber officinale rhizome powder and Bixa Orellana), or of Rovira Virgili, Amposta, Spain
placebo (maltodextrin). Muscle performance was evaluated in
countermovement jump (CMJ) monitored with an accelerome- Introduction: Rate of perceived exertion scale has been pro-
ter (Encoder) and muscle pain through the visual analog scale posed and validated as an effective tool to monitoring tradi-
(VAS) (2) after performing the exercise protocol (3). For statis- tional resistance training (1). The advantages of eccentric-
tical analysis, a mixed ANOVA 5 3 2 or the non-parametric overload (EO) training is the greater larger overload in the
Friedman or the U Mann Whitney tests were applied adjusting eccentric phase and the fact that the resistance can be adap-
with the Bonferroni test (SPSS v.21, IBM, USA). Results: The ted throughout the entire range of movement in the concentric
perception of pain increased after the training session in both phase (2). Various studies have evidenced positive adaptations
Copyright © 2020 National Strength and Conditioning Association. Unauthorized reproduction of this article is prohibited.
Abstracts
in performance with this method type of training (3). We aim to 4 neuromuscular fitness tests on a representative sample of
compare the mean concentric power and RPE of the muscle adult recreationally active women who regularly attend fitness
fatigue on the YoYo-Squat exercise. Methods: Nineteen semi- centers. Methods: Four hundred seven Spanish women (16–
professional soccer players (mean (SD) age = 22.16 6 2.09 69 year old) (38.82 6 10.75 years, 64.20 6 12.04 kg), per-
years; height = 1.77 6 8.21 m; body weight = 73.21 6 8.15 formed 4 tests: knee Push-Ups in one minute, 458 suspended
kg). Participants underwent 3-sets of an EO-squat incremental rows in one-minute, horizontal jump and 3 kg ball throwing. To
test using a fly-wheel machine (Eccopower Training Force, Byo- find out if there were differences in the results obtained
medic System) equipped with a rotatory encoder. RPE was used between different age decades, a one-way ANOVA for each
in order to assess the muscle fatigue of the lower limbs at the test was carried out. Results: No differences between age
end of each set. The study protocol complied with the Declara- ranges were found for Push-Ups (F2,217 = 0.6; p = 0.7)
tion of Helsinki for Human Experimentation and was approved by and suspended rows (F5,171 = 2.185; p = 0.058), whilst
the ethics committee at the institutional review board. Results: differences were found out for horizontal jump (F5,369 =
Significant associations between RPE and mean power at all 17.944; p , 0.001) and ball throwing (F5,312 = 8.301; p ,
levels of inertia were shown (Low inertia: ⍴ = 20.50; p = 0.001). Discussion: The muscular endurance tests for time in
0.03. Intermediate inertia: ⍴ = 20.58; p = 0.01. High inertia: the upper limb do not reflect changes in physical condition in
⍴ = 20.50; p = 0.03). Discussion: RPE may be a useful tool to this population, in contrast to what happens when shorter ef-
monitoring concentric power at EO-squat iso-inertial exercise. forts are made, that a significant difference is perceived from
Despite high correlations between RPE and mean power, an the age of 38 onwards (3). In tests that involve the evaluation of
individualized “power-load profile” should be obtained to pre- neuromuscular power (horizontal jump and ball throw), a nota-
scribe intensity appropriately. References: 1. Helms ER, Cronin ble change is perceived for decades. It is recommended the
J, Storey A, Zourdos MC. Evidence-Based Personal Training The priority use of these 2 tests to assess neuromuscular fitness in
Evidence-Based Personal Training column provides practical physically active women. References: 1. Vallejo NG, Ferrer
application of research topics specific to enh-ancing results RV, Jimena IC, Fernández JA. DP. Valoración de la condición
for personal trainers. Application of the Repetitions in Reserve- fı́sica funcional, mediante el Senior Fitness Test, de un grupo
Based Rating of Perceived Exerti. Strength Cond J [Internet] 38: de personas mayores que realizan un programa de actividad
42–9, 2016. 2. Tesch PA, Fernandez-Gonzalo R, Lundberg TR. fı́sica. Apunts. Educación fı́sica y deportes 2: 22–26, 2004. 2.
Clinical applications of iso-inertial, eccentric-overload (YoYoTM) Ruiz JR, Castro-Piñero J, España-Romero V, et al. Field-based
resistance exercise. Front Physiol 8, 2017. 3. Núñez FJ, Santalla fitness assessment in young people: The ALPHA health-related
A, Carrasquila I, Asian JA, Reina JI, Suarez-Arrones LJ. The ef- fitness test battery for children and adolescents. Br J Sports
fects of unilateral and bilateral eccentric overload training on Med 45: 518–524, 2011. 3. Negrete RJ, Hanney WJ, Kolber
hypertrophy, muscle power and COD performance, and its de- MJ, Davies GJ, Riemann B. Can upper extremity functional
terminants, in team sport players. PLoS One 13: 1–13, 2018. tests predict the softball throw for distance: A predictive
Mail to: antonio.sosa@gmail.com. validity investigation. Int J Sports Phys Ther 6: 104, 2011.
Mail to: ivan600@gmail.com.
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Seville, Seville, Spain; 4Centro de Estudios Universitarios Resistance Training Volume Enhances Muscle Hypertrophy
Cardenal Spı´nola, CEU, Seville, Spain; and 5Department of but Not Strength in Trained Men. Med Sci Sports Exerc 51:
Physical Education and Sports, University of Seville, Seville, 94–103, 2019. Mail to: manueljesus.costillamacias@alum.
Spain uca.es.
Copyright © 2020 National Strength and Conditioning Association. Unauthorized reproduction of this article is prohibited.
Abstracts
F(2,70) = 315.26, p , 0.001, ƞ2 = 0.82). Conclusions: In x time interactions were found (F(2,70) = 10.55, p , 0.001, ƞ2 =
conclusion, our study shows that an elderly population experi- 0.23; F(2,70) = 9.08, p , 0.01, ƞ2 = 0.21, respectively). When the
enced improvements in balance after performing a high intensity dependent variable was the post-intervention score obtained in the
intervention with a TRX training system. References: 1. Blake FES-I test, the ANOVA showed the existence of a main effect of the
AJ, Morgan K, Bendall MJ, Dallosso H, Ebrahim SBJ, Arie THD, intervention group variable (F(2,69) = 6.68, p = 0.002, ƞ2 = 0.16),
Fentem PH, Bassey EJ. Falls by elderly people at home: preva- as well as a statistically significant interaction of group x measure-
lence and associated factors. Age Ageing 17: 365–372, 1988. ment time (F(2,69) = 8.62, p , 0.01, ƞ2 = 0.20). In the ABC test
2. World Health Organization, World Health Organization. Age- revealed a main effect of the intervention group variable (F(2,69) =
ing, & Life Course Unit. WHO global report on falls prevention in 7.13, p = 0.002, ƞ2 = 0.17). Discussion: In conclusion, our study
older age. World Health Organization. 2008. 3. Hita-Contreras shows that an elderly population experienced improvements in
F, Martı́nez-Amat A, Lomas-Vega R, Álvarez P, Aránega A, Mar- sleep quality and fall risk after performing a high intensity interven-
tı́nez-López E, Mendoza N. Predictive value of stabilometry and tion with a TRX training system. References: 1. Muir SW, Gopaul
fear of falling on falls in postmenopausal women. Climacteric 16: K, Montero Odasso MM. The role of cognitive impairment in fall risk
584–589, 2013. Mail to: amamat@ujaen.es. among older adults: a systematic review and meta-analysis. Age
Ageing 41: 299–308, 2012. 2. Brassington GS, King AC, Bliwise
DL. Sleep problems as a risk factor for falls in a sample of
community-dwelling adults aged 64–99 years. J Am Geriatr Soc
EFFECTS OF HIGH INTENSITY SUSPENSION 48: 1234–1240, 2000. 3. World Health Organization, World
TRAINING ON SLEEP QUALITY AND FALL RISK Health Organization. Ageing, & Life Course Unit. WHO global
IN OLDER PEOPLE report on falls prevention in older age. World Health Organization.
2008. Mail to: josedaniel.jimenez@uca.es.
JIMÉNEZ-GARCÍA J.D.,1,2 VELAZQUEZ-DIAZ D.,1,2
HITA-CONTRERAS F.,3 AIBAR-ALMAZAN A.,3 AND
MARTÍNEZ-AMAT A.3
1MOVE-IT
ERYTHROCYTE MEMBRANE DHA DEPLETION
Research group and Department of Physical
AFTER A GRAN FONDO CYCLING RACE: A
Education, Faculty of Education Sciences University of Ca´diz,
PILOT OBSERVATIONAL CASE STUDY
Ca´diz, Spain; 2Biomedical Research and Innovation Institute
of Ca´diz (INiBICA) Research Unit, Puerta del Mar
PIEROTTI S.,1 TORQUATO P.,1 LAROCCA A.V.,1
University Hospital University of Ca´diz, Spain; and
3Department of Health Sciences, Faculty of Health Sciences, AND FERRERI C.1
University of Jae´n, Jae´n, Spain 1Lipidomic Laboratory Lipinutragen srl, Bologna, Italy
Introduction: Risk of falls is one of the main problems related to Introduction: Fatty acid composition defines structural and func-
aging, increasing disability, and morbidity in this population, and it tional properties of cell membranes and is tissue-specific [1]. FA-
causes serious public health problems [1]. Moreover, fall risk based membrane lipidomic profile assesses membrane fluidity
among the elderly has also been associated with sleep disorders through saturated/monounsaturated ratio and PUFA balance index,
[2]. Falls can also result from the side effects of medication used for inflammatory/anti-inflammatory potential through the presence of
the treatment of sleep problems and other medical conditions [3]. omega-6 and omega-3 FAs and the extent of oxidative damages
Objectives: The aim of this study was to compare the effect of through peroxidation index. Athletes’ health status related to nutri-
high and moderate intensity training program consisted of a 12- tion, workout load, metabolism and oxidative status can be inferred
week TRX system program on the sleep quality and fall risk in older analysing mature Red Blood Cell (mRBC) lipidome due to the high
adults. Methods: A total of 82 persons (68.23 6 2.97 years, value of this reporter cell [2]. The impact of a Gran Fondo cycling
75.61% women) finished the study. Sleep quality was assessed race was determined using membrane FA composition of mRBC of
using the Japanese version of the Pittsburgh Sleep Quality Index an endurance-trained, non-elite road cyclist. Methods: mRBC
(PSQI). The Activities-specific Balance Confidence Scale (ABC) membrane phospholipids were analysed for FA profiling (GC-
was used to assess balance confidence and Fear of falling was MS) 6 weeks prior (t0, baseline), 3 days (t1) and 9 weeks after
assessed using the FES-I. In HIIT group the main squat activity with (t2) the Granfondo Sestriere race. After t0 and t1, 250 mg of
suspension system divided into 4 four-minute intervals at an inten- docosahexaenoic acid (DHA)/day, 3 wk/mo, were supplemented.
sity of 90–95% of the maximum HR and in MIIT group their intensity Results: After the race, a 1.8-fold mRBC DHA reduction was
were lower: 70% the maximum HR. Results: Regarding variables observed (t0 = 4.4% and t1 = 2.4% of total FAs). After DHA
sleep latency and sleep disturbances, statistically significant group supplementation, mRBC DHA levels were restored (t2 = 7.3%),
the TM
Copyright © 2020 National Strength and Conditioning Association. Unauthorized reproduction of this article is prohibited.
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being 3-fold higher than t1. Discussion: After the race, erythrocyte injuries, performance, training, anatomy, prevention, somatotype”
membranes undergo acute remodelling with depletion of DHA lev- were selected. Prisma 2009 Flow Diagram (5) was used. Results:
els, consequent increase of omega-6/omega-3 ratio and decrease After 4 filters applied, 1009 articles related to Q-angle and female
of Omega-3 Index for cardiovascular risk [3]. This study highlights sport were found; 171 articles were selected and 36 of those were
lipidomic analysis as a tool to monitor the impact of athletes’ training related to gymnastics. Four particularly movements that could
routines because heart and skeletal muscle preferentially incorpo- cause injury related to the Q-angle have been identified: jump
rate DHA, modulating oxygen consumption, improving fatigue resis- landing, changes in direction, high jumps with kicks and exercises
tance and neuromotor function [4,5]. Lipidomic analysis can be in which the knee supports weight while 308 flexion angle. Discus-
applied to personalise lipid supplementation and verify the improve- sion and Practical Applications: The proposal of preventive
ment of performance and recovery after exercise and races. Refer- exercises was based on a work of changes of the direction with
ences: 1. Lauritzen L, Hansen HS, Jørgensen MH, Michaelsen KF. coordination exercises and the correction and improvement of the
The essentiality of long chain n-3 fatty acids in relation to develop- jump technique, with the potentiation of the hamstrings and quad-
ment and function of the brain and retina. Prog Lipid Res 1–94, riceps muscles with exercises that increase the knee flexion and hip
2001. 2. Ferreri C, Chatgilialoglu C. Role of fatty acid-based func- flexion/extension balance. Moreover, exercises that decrease the
tional lipidomics in the development of molecular diagnostic tools. valgus of the knee increasing the external rotation of the hip with
Expert Rev Mol Diagn 12: 767–780, 2012. 3. Harris WS. Omega- the potentiation of the gluteus muscles group, specially the medial
3 Fatty Acids and Cardiovascular Disease: A Case for Omega-3 gluteus. In addition, the work of the core and supination and dorsi-
Index as a New Risk Factor. Pharmacol Res 55: 217–223, 2007. flexion exercises for the ankle helps to the balance of the knee.
4. Pepe S, McLennan PL. Cardiac membrane fatty acid composi- References: 1. Vernetta M, Montosa I, López-Bedoya J. Rev Andal
tion modulates myocardial oxygen consumption and postischemic Med Deporte 9:105–109, 2016. 2. Oltean A, Rusu MM, Copoiu N,
recovery of contractile function. Circulation 105: 2303–8, 2002. Calin MF. Sci Mov Health 17:427–432, 2017. 3. Abalo-Núñez R,
5. Peoples G, McLennan P. Dietary fish oil reduces skeletal muscle Gutiérrez-Sánchez A, Iglesias PM, Vernetta-Santana M. Sci Sports
oxygen consumption, provides fatigue resistance and improves 33: 228–236, 2018. 4. Livingston LA. J Orthop Sports Phys Ther
contractile recovery in the rat in vivo hindlimb. Br J Nutr 104: 28: 105–109, 1998. 5. Moher D, Liberati A, Tetzlaff J, Altman DG,
1771–1779, 2010. Mail to: alivis.p@gmail.com. The PRISMA Group. PLoS Med 6: e1000097, 2009. Mail to:
monica.hontoria@upm.es.
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Abstracts
gained increasing interest in sports science (1). The aim of this Introduction: Asymmetries can lead to muscular imbalances or
study was to analyze the kinetics of HRV 72 hours after per- deficits in strength during some activities [1]. However, little is
forming an acute exercise that induces DOMS. Methods: A known while performing water fitness exercises. This study
descriptive study was conducted in which 41 healthy and ath- aimed to assess the upper-limbs’ asymmetry in water fitness
lete’s males participated, who performed the eccentric exer- exercises. Methods: Thirty-two subjects performed the horizon-
cise protocol described by Howatson (2) to induce DOMS. tal adduction (HA) and the rocking horse with horizontal adduc-
The analysis of the HRV was carried out before, immediately tion (RHAdd) over an incremental protocol, starting between
after, and at 24, 48 and 72 hours after the acute exercise, 105 and 150 b$min-1. A differential pressure sensor system
using the Ambit3 monitor (Suunto, Amer Sports, Strava, Fin- (Aquanex, 4.1, STR, USA) was used to collect the propulsive
land). The NN intervals were analyzed using Kubios HRV soft- forces (PF) on dominant (D) and non-dominant (ND) upper-limb.
ware (version 3.1.0) to measure standard deviation of all NN Symmetric Index (SI, %) was estimated and interpreted as pro-
intervals (SDNN) as a metric of a total variability and ratio of the posed by Robinson, Herzog and Nigg [2]. Results: A symmetric
low to high frequency power (HF/LF) as a metric of sympatho- motion was found for the HA at 105 (10.94 6 7.43%), 120
vagal balance. For the statistical analysis the non-parametric (10.77 6 7.54%) and 135 b$min21 (9.57 6 5.50%), except at
tests of Friedman and Wilcoxon were applied, adjusting with 150 b$min21 (11.95 6 7.44%). The RHadd elicited an asym-
the Bonferroni test (SPSS v.21, IBM, EEUU). Results: SDNN metric motion at the majority of the selected cadences (RH
decreased after the acute exercise session (p , 0.001), recov- 105–12.76 6 9.91%; RH 120–14.84 6 9.15%; RH 150–
ering its initial values at 24 hours after the intervention. In addi- 14.64 6 10.54%), except for the 135 b$min21 (10.63 6
tion, the variability exceeded the initial values at 72 hours (p , 8.00%). DHA was the largest at 105 (68.75%) and 120
0.003). HF/LF ratio reflects an increase in sympathetic activity b$min21 (59.38%). At 135 and 150 b$min21 the trend was
after exercise (p , 0.007) to recover the vagal sympathetic for a similar amount of force for both sides (DHA - 50%; NDHA
balance at 24 hours. Discussion: The behavior of the HRV - 50%). The RHadd had a greater PF applied in DRH at 105
indicates that at 24 hours after an acute exercise the auto- (56.25%), 135 (59.38%) and 150 b$min21 (53.13%), whereas
nomic nervous system recovers the initial baseline values, pre- 120 b$min21 seems recruited more the NDRH (56.25%). Dis-
senting a higher HRV at 72 hours, which could be reflecting cussion: Exercises involving multiple hops led to asymmetries
a neuromuscular adaptation that is assumed by the DOMS when comparing with static positions (e.g., HA). Most actions
process. (3). Practical Applications: The determination of were asymmetric except for the 135 b$min21 cadence that elic-
HRV to athletes is a non-invasive technique that could give its symmetric motion in both exercises. Faster cadences elicited
coaches accurate information on when the athlete is recovered a larger percentage of the total subjects that applied more PF in
for a new session, avoiding overtraining episodes that DRH, while the same pattern was found in lower cadences for
decrease the performance of athletes. References: 1. Hotten- DHA. Water fitness instructors should consider the 135
rott K, et al. Heart rate variability and physical exercise. Current b$min21 cadence as a key to the development of strength.
status. Herz. septiembre de 2006. 2. Howatson G, et al. References: 1. Sanders R, Thow J, Fairweather M. Asymme-
Exercise-induced muscle damage is reduced in resistance- tries in swimming: Where do they come from? J Swim Res 18,
trained males by branched chain amino acids: A randomized, 2011. 2. Robinson R, Herzog W, Nigg B. Use of force platform
double-blind, placebo controlled study. J Int Soc Sports Nutr variables to quantify the effects of chiropractic manipulation on
2012. 3. Hody S, et al. Eccentric Muscle Contractions: Risks gait symmetry. J Manipulative Physiol Ther 10: 172–176, 1987.
and Benefits. Front Physiol 2019. Mail to: andreabasfdz@ Mail to: catarina.costa.santos@ubi.pt.
gmail.com.
Faculty of Sports Science and Physical Education, University Universitat Rovira i Virgili (URV), Amposta, Spain; and
2Grup d’investigacio´ LIFE, Universitat Jaume I (UJI),
of Coimbra, Portugal; Department of Sport Sciences, University
of Beira Interior, Covilha˜, Portugal Castello´n, Spain
the TM
Copyright © 2020 National Strength and Conditioning Association. Unauthorized reproduction of this article is prohibited.
the TM
Introduction: Scientific evidence has suggested that sleeps (3.1 vs. 5.3; p , 0.001) at experimental group after the inter-
patterns may influence sports performance [1]. Nonetheless, vention. However, no changes were found in sleep quantity.
intervention research on this topic is limited. The aim of this Conclusions: Eight-week sleep hygiene interventions may
study was to analyze the effects of a sleep hygiene intervention improve sleep quality of young gymnasts. References: 1. Bon-
on sleep quantity and quality of young gymnasts. Methods: nar D, Bartel K, Kakoschke N, Lang C. Sleep Interventions
Twenty gymnasts were allocated in either control group or Designed to Improve Athletic Performance and Recovery: A
experimental group (n = 10). Following a familiarization period, Systematic Review of Current Approaches. Sport Med 48:
an 8-week intervention was applied. The intervention consisted 603–703, 2018. 2. Macı́as Fernández JA, Royuela A. La ver-
of reading infographics weekly plus a complementary talk of sión española del Índice de Calidad de Sueño de Pittsburgh.
sleep hygiene every 2 weeks. Sleep duration was self-reported Inf Psiquiatr 146: 465–72, 1996. 3. Caia J, Scott TJ, Halson
by the participants and sleep quality was measured through the SL, Kelly VG. The influence of sleep hygiene education on
Pittsburgh Sleep Quality Index (PSQI) questionnaire [2]. The sleep in professional rugby league athletes. Sleep Heal 4:
study protocol complied with the Declaration of Helsinki for 364–8, 2018. 4. O’Donnell S, Driller MW. Sleep-hygiene
Human Experimentation and was approved by the ethics com- Education improves Sleep Indices in Elite Female Athletes.
mittee at the institutional review board. Written informed con- Int J Exerc Sci. 10: 522–30. 5. Van Ryswyk E, Weeks R,
sent was obtained from each participant and their parents/legal Bandick L, O’Keefe M, Vakulin A, Catcheside P, et al. A novel
tutors in advance. Results: Improvements in sleep quality were sleep optimisation programme to improve athletes’ well-being
found (3.1 vs. 5.3; p , 0.001) at experimental group after the and performance. Eur J Sport Sci 17: 144–51, 2017. Mail to:
intervention. However, no changes were found in sleep quan- albaencina123@hotmail.com.
tity. Discussion: Improvements in sleep quality were found
Copyright © 2020 National Strength and Conditioning Association. Unauthorized reproduction of this article is prohibited.