APPENDICES

APPENDIX A

Reducing Sugar Estimation by Dinitrosalicylic Acid Method (Wood et al., 1988)

Dinitrosalicylic acid, 40 g

Phenol, 8 g

Sodium sulfite, 2 g

Rochelle salt, 800 g

All are dissolved in 2 liters of 2% (w/v) NaOH solution and then diluted to 4 liters

with distilled water.

Glucose standard (2.0 mg/mL)

Procedure:

1. Diluted sugar solution (1.5 mL) to be assayed is mixed with 3 mL DNS

reagent.

2. Placed in a boiling water bath for 5 min and cooled to room temperature.

3. Read the absorbance at 540 nm.

 APPENDIX FIGURES

Figure 1. Isolates streaked on MHA supplemented with 1% CMC

Figure 3. Submerged fermentation set-up

 Figure 4. Enzyme assay set-up

APPENDIX B
Table 1. Tukey HSD test on the enzyme activity between and among treatments
Substr Isola {1} - . {2} - . {3} - . {4} - . {5} - . {6} - . {7} - . {8} - . {9} - .
ate te 00002 00002 00003 00003 00003 00003 00003 00002 00002
0.99991 0.98828 0.99882 0.94256 0.99948 0.99538 1.00000 1.00000
1 1
0 7 2 2 1 6 0 0
0.99991 0.88860 0.96304 0.75154 0.97467 0.92955 0.99989 0.99973
1 2
0 8 5 9 4 5 7 4
0.98828 0.88860 0.99999 0.99999 0.99999 1.00000 0.98895 0.99307
1 3
7 8 9 7 2 0 2 1
0.99882 0.96304 0.99999 0.99964 1.00000 1.00000 0.99892 0.99949
2 1
2 5 9 6 0 0 6 1
0.94256 0.75154 0.99999 0.99964 0.99915 0.99996 0.94467 0.95893
2 2
2 9 7 6 4 1 8 0
0.99948 0.97467 0.99999 1.00000 0.99915 1.00000 0.99953 0.99980
2 3
1 4 2 0 4 0 4 3
0.99538 0.92955 1.00000 1.00000 0.99996 1.00000 0.99570 0.99759
3 1
6 5 0 0 1 0 8 6
1.00000 0.99989 0.98895 0.99892 0.94467 0.99953 0.99570 1.00000
3 2
0 7 2 6 8 4 8 0
1.00000 0.99973 0.99307 0.99949 0.95893 0.99980 0.99759 1.00000
3 3
0 4 1 1 0 3 6 0

Table 2. Tukey HSD test on the enzyme activity between substrates

Substrate {1} - .00002 {2} - .00003 {3} - .00003
1 0.494741 0.968160
2 0.494741 0.640457
3 0.968160 0.640457

Table 3. Tukey HSD test on the enzyme activity between isolates

Isolate {1} - .00003 {2} - .00003 {3} - .00003
1 0.914775 0.997202
2 0.914775 0.883735
3 0.997202 0.883735