Xcal - TriPlus RSH User Guide

Thermo Xcalibur
TriPlus RSH Autosampler Setup

User Guide
P/N 31709650, Revision B, March 2013

© 2013 Thermo Fisher Scientific Inc. All rights reserved.
Xcalibur and TriPlus RSH are registered trademarks of Thermo Fisher Scientific Inc. in the United
States.Windows is a registered trademark of Microsoft Corporation in the United States and other countries.
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Vista, and Windows 7 are trademarks of Microsoft Corporation. Acrobat is a trademark of Adobe Systems. All
other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-
free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
result from any use of this document, even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of
Sale shall govern all conflicting information between the two documents.
Release history: Revision A, September 2011; Revisione B, March 2013
The Xcalibur manuals support Xcalibur version 2.2 and above.
For Research Use Only. Not for use in diagnostic procedures.

C
Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
System Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .viii
Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .viii
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .ix
Chapter 1 TriPlus RSH Configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Instrument Configuration Dialog Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Sequence Error Handling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Chapter 2 TriPlus RSH Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5

Road Map Home Page Status Tabs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Status View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Virtual Terminal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Chapter 3 TriPlus RSH Instrument Setup Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9

Menu Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
New Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Load Method Template. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Save Method Template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Load Custom Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Start Fiber Conditioning Cycle Off-line . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Start Syringe Clean Cycle Off-line. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Inject off-line samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Read Bar Code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Stop Sample Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Validate Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Edit Sampler Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Show Status View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Show Virtual Terminal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Show Tray Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Start Fiber Conditioning Cycle Off-line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Conditioning Port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Fiber Conditioning Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
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Contents
Start Syringe Clean Cycle Off-line. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Washes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Inject Off-line Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Module for Racks/Wells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
List of Added Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Chapter 4 Instrument Method Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21

Instrument Method Page. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Configuring GC Liquids Method Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Configuring GC Headspace Method Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Configuring SPME Method Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Creating a Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Chapter 5 GC Liquids Method Parameters Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27

GC Liquids Method Setup - General Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Injection Port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Injection Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Rapid Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Syringe Type . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Sampling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Sample Depth in Vial . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Sample Viscosity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
GC Liquids Method Setup - 2nd Inj.Port Page . . . . . . . . . . . . . . . . . . . . . . . . . 33
Injection Port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
GC Liquids Method Setup - Washes Page. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Washes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Pre-Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Rinse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Post-Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
GC Liquids Method Setup - Synch Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
GC Synchro Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
GC Liquids Method Setup - Synch / Int. Std Setup Page . . . . . . . . . . . . . . . . . 38
GC Synchro Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Internal Standard. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Internal Standard Depth in Vial . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
GC Liquids Method Setup - Synch / Solvent Flush Page. . . . . . . . . . . . . . . . . . 40
GC Synchro Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Solvent Flush. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
GC Liquids Method Setup - Enrichment Page . . . . . . . . . . . . . . . . . . . . . . . . . 42
Enrichment Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
GC Liquids Method Setup - Advanced Page . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Advanced Parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
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Contents
GC Liquids Method Setup - Cooled/Heated Tray Holder Page . . . . . . . . . . . . 44

Primary/Secondary Tray Temperature. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Chapter 6 GC Headspace Method Parameters Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45

GC Headspace Method - General Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Injection Port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Syringe Type . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Analysis Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Incubation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Sample. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Sampling Depth in Vial. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
DoublePro 2nd Injector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
GC Headspace Method - Syringe Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
GC Headspace Method - Syringe Solvent Washes Page. . . . . . . . . . . . . . . . . . . 52
Washes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
GC Headspace Method - Synchronization/Stand-by Page . . . . . . . . . . . . . . . . . 53
Wait for Readiness Flags . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Stand-by Syringe Flush . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
GC Synch Start Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Anticipated Synchronization Before Incubation End . . . . . . . . . . . . . . . . . . . 54
GC Headspace Method - Advanced Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Advanced Parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Chapter 7 SPME Method Parameters Setup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57

SPME Method Setup - Parameters Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Desorb Port. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Sample Vial Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Sampling Vial Depth. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Incubation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Analysis Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
GC Synch Start Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
SPME Method Setup - Fiber Conditioning Page. . . . . . . . . . . . . . . . . . . . . . . . 61
Derivatization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Conditioning Port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Fiber Conditioning Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Chapter 8 Custom Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63
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Contents
vi manual-name Thermo Scientific

P
Preface
This guide provides the instruction for the control of the TriPlus RSH autosampler through
Xcalibur.
Contents
• Related Documentation
• System Requirements
• Safety and Special Notices
• Safety and Special Notices
• Contacting Us
Related Documentation
Thermo Fisher Scientific provides these documents for Xcalibur:
• Xcalibur Getting Started (Quantitative Analysis)
• Acquisition and Processing User Guide
• Quantitative Analysis User Guide•Qualitative Analysis User Guide
• Creating and Searching Libraries User Guide
• XReport User Guide
• Xcalibur System and 21 CFR Part 11 Compliance Administrator Guide
• Help from within the software
 To view product manuals
Go to Start > Programs > Thermo Instruments | Manuals | <Product Name>
Thermo Scientific manual-name vii

Preface
System Requirements
 To open Help
• From the product-name window, choose Help > product-name Help.

• If available for a specific window or dialog box, click Help or press F1 for
information about setting parameters.
For more information, including upcoming application notes, visit

www.thermoscientific.com.
System Requirements
Xcalibur requires a license. In addition, your system must meet these minimum requirements.
System Requirements
PC • 2.4 GHz processor with 4GB RAM
• DVD/CD-ROM drive
• Video card and monitor capable of 1280x1024 resolution (XGA)
• 250 GB or greater available on the C: drive
• NTFS format
Software • Microsoft® Windows® XP Professional with Service Pack 3,
• Windows 7 Professional (32-bit) with Service Pack 1
• Microsoft Office™ 2007 or 2010
• Thermo Foundation 2.0 SP1 or higher (Thermo Scientific software)
• Xcalibur™ 2.2 or higher
Safety and Special Notices

Make sure you follow the precautionary statements presented in this guide. The safety and
other special notices appear in boxes.
Safety and special notices include the following:
CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION

notice is accompanied by an appropriate CAUTION symbol.
viii manual-name Thermo Scientific

Preface
Contacting Us
IMPORTANT Highlights information necessary to prevent damage to software, loss of

data, or invalid test results; or might contain information that is critical for optimal
performance of the system.
Note Highlights information of general interest.
Tip Highlights helpful information that can make a task easier.
Contacting Us
Thermo Fisher Scientific provides comprehensive technical assistance worldwide and is
dedicated to the quality of our customer relationships and services.
Use http://www.thermoscientific.com address for products information.address for products

information.
Use http://www.gc-gcms-customersupport.com/WebPage/Share/Default.aspx address to

contact your local Thermo Fisher Scientific office or affiliate GC-GC/MS Customer Support.
Thermo Scientific manual-name ix

Preface
Contacting Us
x manual-name Thermo Scientific

1
TriPlus RSH Configuration

This chapter contains the instructions to configure your TriPlus RSH for use with Xcalibur.
Contents
• Instrument Configuration Dialog Window
• Connection
• Mode
• Sequence Error Handling
Thermo Scientific TriPlus RSH Autosampler Setup 1

1 TriPlus RSH Configuration
Instrument Configuration Dialog Window

This dialog window is available from the Instrument Configuration window.
To open this view choose Start | Programs | Thermo Foundation | Instrument

Configuration.
Click on the TriPlus RSH button located in the Available Devices column. Click Add, then
Configure button. The TriPlus RSH Configuration window is visualized. See Figure 1.
Figure 1. TriPlus RSH Configuration Dialog Window
Configuration windows is subdivided into the following fields:

• Connection
• Mode
• Sequence Error Handling
Configuration window includes the following common buttons:

• Ok — Use this button to close the dialog box and confirm your selection.
• Cancel — Use this button to clear all modifications.
Connection
In this field you set the LAN communication between the TriPlus RSH and the data system.
The parameters are described below.
2 TriPlus RSH Autosampler Setup Thermo Scientific

Network Address
Enter the I.P. address to allow the LAN control of the TriPlus RSH through the Thermo data
systems.
The TriPlus RSH is shipped with a default IP address, which may not match the needs of the
LAN where the autosampler must be installed. To change the default values, contact your
LAN administrator and ask the IP address to be assigned, the netmask, and eventually the
port.
• The IP address is a 3 digits x 4 fields given by the network administrator e.g.
192.168.127.10
• The netmask is a 3 digits x 4 fields given by the network administrator e.g.
255.255.255.0
• The port is a 4 digits number given by the network administrator e.g. 4001 (4001 is the
CPU/LAN default).
• The default IP address of the TriPlus RSH CPU is 192.168.99.230
Mode
This field includes the following parameters:
Instrument
Configure the sampler to work with two different Data Systems, with each of them using its
own method and sequence of samples.
Configure the sampler as one of the following:

• Main — Select this option to configure the sampler as main instrument.The start signal is
generated at the output of the GC1 connector.
• Virtual (Secondary) — Select this option to operate with a second GC, controlled by a
second independent data system. The start signal is generated at the output of the GC2
connector.
IMPORTANT Main and Virtual functions are usable only with the TriPlus RSH in GC
Liquids mode.
In case of TriPlus RSH in GC Headspace and SPME modes, the Virtual function is not
usable. To inject in two GC units it is necessary to use the Constant DoublePro
incubation mode connecting the two start/stop cables to the two GC units separately.

Disable hand-held Control

Check this box if you want to disable the control from the Handheld Controller when
available.
Extract the Injection Volume from the Sequence

Check this box if you want using the injection volume set in the Xcalibur sample sequence
instead of the injection volume set in the Instrument Method
If not checked, the injection volume of the method is used.
Read Barcode
Check this box if you want the TriPlus RSH to read the sample barcode attached to the vial.
Sequence Error Handling

Configure how the TriPlus RSH will handle sequence errors such as missing vial.
• Error Mode — By default a recoverable error, such as missing vial, missing solvent, or
missing injector, a fake injection will be performed. There are cases in which the sequence
should not continue but instead operator intervention may be required.
Select the desired error handling option. The three possible selections are listed:
– Skip any error, and do fake injection
– Skip missing vial, and do fake injection
– Stop sequence for all errors

2
TriPlus RSH Status

This chapter describes how you can check on the current status of the TriPlus RSH while
working in Xcalibur.
Contents
• Road Map Home Page Status Tabs
• Status View
• Virtual Terminal

2 TriPlus RSH Status
Road Map Home Page Status Tabs
Road Map Home Page Status Tabs

Status pages are located on Xcalibur Roadmap-Home page. Just highlight TriPlus RSH from
the Roadmap Status tab scroll list and see the TriPlus RSH Autosampler tab. See Figure 2.
Figure 2. Status Tab
This tab comprises the following options:

• Status View
• Virtual Terminal
Status View
Select this option to display the current status, the firmware (F/W) and the hardware (H/W)
version of the autosampler.
A virtual image of the autosampler is displayed with all the components currently installed
and the syringe data.

Virtual Terminal
Virtual Terminal
Select this option to display the Virtual Terminal. See Figure 3.
Figure 3. Virtual Terminal
Close Button
Status View
Option Key
Default Option Key
Enter Key
Scroll Wheel
The virtual terminal is used to directly interact with the TriPlus RSH. The virtual terminal
operates in a similar fashion to the handheld controller option (when available).
• Back Key — Returns to the previous menu. If Back button is pressed continuously,, the
cursor will jump back to the Home screen (Start Page).
• Stop Key — Aborts the ongoing activities as soon as possible.
• Scroll Wheel — Rotate the outer knob to scroll through items in a menu.
• Enter Key — To select an highlighted item.
The control of the software is designed with three different Access Levels.
• User — The default level.
• Extended — The level for advanced operation of the sytem.
• Service — This level is for the service technician only.
The purpose of this distinction is to display only specific items and sections of the firmware
for each level.

Virtual Terminal
Using the Virtual Terminal, the Access Level can be changed by pressing the two keys A and B
on the computer keyboard at the same time.

3
TriPlus RSH Instrument Setup Menu

This chapter describes the Instrument Setup menu.
Contents
• Menu Description
• New Method
• Load Method Template
• Save Method Template
• Load Custom Method
• Start Fiber Conditioning Cycle Off-line
• Start Syringe Clean Cycle Off-line
• Inject Off-line Samples
• Read Bar Code
• Stop Sample Operation
• Validate Method
• Show Status View
• Show Virtual Terminal
• Show Tray Configuration

3 TriPlus RSH Instrument Setup Menu
Menu Description
Menu Description
The TriPlus RSH menu is located on the Instrument Setup menu bar. See Figure 4.
Figure 4. TriPlus RSH Menu
The pull-down menu contains the following options:

• New Method
• Load Method Template
• Save Method Template
• Load Custom Method
• Start Fiber Conditioning Cycle Off-line
• Start Syringe Clean Cycle Off-line
• Inject off-line samples
• Read Bar Code
• Stop Sample Operation
• Validate Method
• Edit Sampler Modules
• Show Status View
• Show Virtual Terminal
• Show Tray Configuration

Menu Description
New Method
This function creates a new sampler method starting from the data currently in memory or
from the default parameters.
Load Method Template

This function lets the user select a method template from available pre-installed system
templates or user-created method templates. The system asks for the Sampler method name.
All volumes, clean cycles and injection parameters stored in the given filename are
automatically loaded in memory.
Save Method Template

This function allows the user to save a sampler analytical method as a method template. It is
useful when several methods having a similar configuration must be created. You may find the
template method by using New Method option.
Load Custom Method

Select a custom method, which includes a means of creating a method, for example for sample
preparation and dilution steps. Custom methods can be loaded as needed to support these
operations.
Start Fiber Conditioning Cycle Off-line

This function is available only for the TriPlus RSH for SPME instrument.You can set
parameters for offline fiber conditioning. Refer to “Start Fiber Conditioning Cycle Off-line”
on page 15.
Start Syringe Clean Cycle Off-line

This selection starts a syringe cleaning cycle off-line. Refer to “Start Syringe Clean Cycle
Off-line” on page 16.
Inject off-line samples

Select this option to inject a sample off-line. See “Inject Off-line Samples” on page 18.
Read Bar Code

By selecting this option, the TriPlus RSH will read the barcode on the selected sample.

Menu Description
Stop Sample Operation

This function interrupts any current operation on the autosampler.
Validate Method
Select this function to check the compatibility between the analytical method and the system.
If the method is compatible, the message Method is valid will be displayed. See Figure 5.
Figure 5. Method Valid Message.
In the event of an invalid method, verify and correct the configuration of your system and the
parameters settings.
Edit Sampler Modules

Select this option to show the sampler modules set in the Instrument Setup according to the
configuration mode GC Liquids, GC Headspace or SPME. Edit the sampler modules as in
Instrument Setup. See Figure 6.
Note You must complete this step for each new method you create.
Figure 6. Sampler Modules Dialog Box

Menu Description
Show Status View

Select this option to show the virtual view of the sampler. See Figure 7.
Figure 7. Status View: Autosampler Tab
The Autosampler tab shows the image of your autosampler with all the components currently
installed and their movements in real time.
The Overlapping tab schematically shows the numbering overlapping of the samples.
In the lower section of the page, the module type, the parameter type and its value are
displayed. The status line indicates the current status of the instrument.

Menu Description
Show Virtual Terminal

Select this option to show the virtual Handheld Controller. Refer to Virtual Terminal for
details.
Figure 8. Virtual Terminal
Show Tray Configuration

Select this option to show the configuration of the tray. In Module for Racks/Wells box,
select the module configured. Choose StackDrawer or TryHolder accordingly. See the
example of Figure 9.
Figure 9. Example of Tray Configuration Dialog Box
• In Rack Type box select the relevant type rack installed.

• In Vial Type in Rack box select the type of vial inserted in the relevant rack.

This dialog window is available only for the TriPlus RSH in SPME mode.
Figure 10. Start Fiber Conditioning Dialog Window
The fields and the buttons in this page are described below.
• Conditioning Port
• Fiber Conditioning Station
Conditioning Port
This field contains parameters for Syringe ID, Port ID, and Desorption Time (min).
• Syringe ID — Select the type of SPME fiber to use.
• Port ID — Select the injector or fiber conditioning station to use for the fiber bake out.
• Desorption time (min) — Set the desorption time in minutes of the fiber in the selected
conditioning port. The range is from 0.1 to 600.
Fiber Conditioning Station

This field contains the parameter Conditioning Temperature (°C).

• Conditioning Temperature (°C) — Set the conditioning temperature in °C of the fiber

conditioning station. The available values for the temperature are in the range from 40 °C
to 350 °C.

Selecting this action allows you to perform a syringe cleaning cycle off-line. The following
dialog box is displayed.
Figure 11. Syringe Clean Dialog Box
The field in this page is described below.

• Washes
Washes
This field comprises the following parameters:
• Syringe ID — Select in this box the syringe type to wash.
• Number of solvent(s) — Select in this box the number of solvent to use for the washing
cycle. Choose between Single and Multiple.
– Select Single to enable single syringe solvent wash.
– Select Multiple for syringe solvent wash by using up to four solvents.
• Wash Station ID — Select in this box the type of washing station installed.

• Cleans — This parameter specifies the number of cleans for syringe solvent wash. The
range is from 0,1 to 15.
• Solvents — The parameter specifies the solvent(s) for syringe solvent wash. The range is
A, B, C and D, for each solvent, depending on the type of washing station configured on
the autosampler.
• Solvent Volume (μl) — This parameter specifies the volume in microliters of solvent for
syringe solvent wash. The range depends on the syringe volume.

Inject Off-line Samples

This function allows to start the sequence of a batch of samples. Figure 12 shows an example
of the Inject Off-line Samples dialog window displayed.
Figure 12. Sample Injection Off-line
The fields in this page are described below.

• Module for Racks/Wells
• List of Added Samples
• Buttons
Related Topic
• To Inject Off-line Samples
Module for Racks/Wells

In Module for Racks/Wells box, choose the module where the sample vials are located. The
graphical image of the numbered vials located in the module selected is visualized accordingly.
List of Added Samples

This box lists the number and the order sequence of all the samples to inject.

Buttons
• Add Selected Sample — Click on this button to add the selected sample vial in the List
of Added Samples box.
• Move Up and Move Down — Click on this button to modify the sequence order of the
sample to inject listed in the List of Added Samples box.
• Remove — Click on this button to remove a selected sample from the List of Added
Samples box.
• Clear All — Click on this button to clear the List of Added Samples box.
• Inject — Click on this button to start the sequence.
 To Inject Off-line Samples
1. In Module for Racks/Wells box, choose the module where the sample vial are located.
2. On the graphical image of the module, select the number of the first sample to inject.
3. Click on the Add Selected Sample button. The selected sample is added in the List of
Added Samples box.
4. Repeat step 2 and step 3 until all the samples to inject are listed in the List of Added
Samples box.
5. When ready, click on the Inject button to start the sample sequence.


4
Instrument Method Setup

This chapter provides instruction to create and save an Xcalibur instrument method for the
TriPlus RSH
Contents
• Instrument Method Page
• Configuring GC Liquids Method Mode
• Configuring GC Headspace Method Mode
• Configuring SPME Method Mode
• Creating a Method

4 Instrument Method Setup
Instrument Method Page
Instrument Method Page

You can set a TriPlus RSH instrument to work in any one of three available modes namely
GC Liquids (AS), GC Headspace (HS) and SPME on the Instrument Setup page. See
Figure 13.
Figure 13. Method Setup Page
Use this page also to configure the modules installed on your TriPlus RSH. The modules to
configure vary depending on the working mode associated.
• Configuring GC Liquids Method Mode
• Configuring GC Headspace Method Mode
• Configuring SPME Method Mode

Configuring GC Liquids Method Mode
Configuring GC Liquids Method Mode

In this page you configure the modules associated to the GC Liquids method mode. See
Figure 14. Also refer to GC Liquids Method Parameters Setup.
Figure 14. GC Liquids Method Setup Page
This page comprises the following fields:

• Method — In this field select the GC Liquids method mode.
• Injectors — In this field use the Injector Ports A/B/C/D list boxes to select the injectors
to use.
• Syringes — In this field use the Syringe-ID list box to select the syringe.
• Wash Station — In this field use the Wash Station-ID list box to select the washing
station to use.
• Internal Standard / Solvent Station — In this field use the relevant list box to select the
internal standard and the solvent station to use.
• Cooled / Heated Trays — In this field use the relevant list box to select the cooled/heated
Primary Tray holder and the cooled/heated Secondary Tray holders.

Configuring GC Headspace Method Mode
Configuring GC Headspace Method Mode

In this page you configure the modules associated to the GC Headspace method mode. See
Figure 15. Also refer to GC Headspace Method Parameters Setup.
Figure 15. GC Headspace Method Setup Page

• Method — In this field select the GC Headspace method mode.
• Injectors — In this field use the Injector Ports A/B/C/D list boxes to select the injectors
to use.
• Wash Station — In this field use the Wash Station-ID list box to select the washing
station to use.
• Agitator and Adapter — In this field use the relevant list box to select the headspace
agitator (incubation oven) and the multiple headspace extraction (MHE) adapter to use.

Configuring SPME Method Mode
Configuring SPME Method Mode

In this page you configure the modules associated to the SPME (Solid Phase Micro
Extraction) method mode. See Figure 16. Also refer to SPME Method Parameters Setup.
Figure 16. SPME Method Setup Page

• Method — In this field select the SPME method mode.
• Injectors — In this field use the Injector Ports A/B/C/D list boxes and the Fiber
Conditioning Port to select the injection ports to use.
• Internal Standard Station — In this field use the Internal Standard Station-ID list box
to select the station to use.
• Agitator — In this field use the Agitator-ID list box to select the agitator (incubation
oven) to use.

Creating a Method
Creating a Method
To create a method proceed as follows:
1. Select the method mode among GC Liquids, GC Headspace and SPME.
2. Configure the related modules.
3. Click the Create a Method button.
The instrument methods and parameters settings for the methods vary depending on the
working mode associated.
According to the method mode of your TriPlus RSH, click on the appropriate topic to open
the relevant Method Setup page.
• GC Liquids Method Parameters Setup
• GC Headspace Method Parameters Setup
• SPME Method Parameters Setup

5
GC Liquids Method Parameters Setup

This chapter covers information for your TriPlus RSH autosampler working in GC Liquids
method mode.
The GC Liquids Method Parameters Setup pages are available if you have selected GC
Liquids as method mode in the Instrument setup. Refer to Configuring GC Liquids Method
Mode.
On the right section of the GC Liquids Method Setup - General Page, the syringe filling is
schematized according to the injection mode selected. A specific parameter corresponds to
every colored segment.
GC Liquids Method Setup Page is composed of several subsidiary pages (tabs) some of which
will only be visualized following the selected options. According to the type of injector, the
syringe installed and the desired injection mode, the tab will show the suggested values that
can be changed by the operator.
GC Liquids Method Parameters Setup comprises the following tabs:

• GC Liquids Method Setup - General Page
• GC Liquids Method Setup - 2nd Inj.Port Page
• GC Liquids Method Setup - Washes Page
• GC Liquids Method Setup - Synch Page
• GC Liquids Method Setup - Synch / Int. Std Setup Page
• GC Liquids Method Setup - Synch / Solvent Flush Page
• GC Liquids Method Setup - Enrichment Page
• GC Liquids Method Setup - Advanced Page
• GC Liquids Method Setup - Cooled/Heated Tray Holder Page

5 GC Liquids Method Parameters Setup
GC Liquids Method Setup - General Page

Plan in this page the parameters of sampling and injection.
Figure 17. GC Liquids Method Setup - General Page
This page comprises the following fields

• Injection Port
• Injection Mode
• Rapid Mode
• Syringe Type
• Sampling
• Injection
• Sample Depth in Vial
• Sample Viscosity
Injection Port
This field comprises the following options:
• Injector — Select the injector to use in the method choosing among the options listed in
the combo box.

• Type — Select the type of injection type choosing between the following options:
– Single: Select the option when the sampling will be performed in a single injector.
– Double: Select this option when the sampling will be performed in two injectors. In
this case the 2nd Inj. Port page will be displayed where the required parameters for
the second injector should be set. Refer to GC Liquids Method Setup - 2nd Inj.Port
Page.
Injection Mode
Select the injection Mode of interest choosing among the options listed in the combo box.
• Basic
• Enrichment
• Enrichment needle solvent wash
• Internal standard double
• Internal standard post
• Needle solvent wash
• Solvent flush double
• Solvent flush post
The syringe filling scheme, displayed on the right side of the page, depends on the selected
injection mode. Each colored segment represents a component of the syringe filling (air,
Internal Standard, sample, solvent etc.) and characterizes the relevant parameter (see
Figure 18).
Figure 18. Syringe Filling Scheme for the Various Injection Modes

Rapid Mode
This parameter specifies the setting for the wait ready signal from the GC after having
performed the preliminary operations. This option is disabled when serving two GC units.
Select the Rapid Mode of interest choosing among the options listed in the combo box.
• Disabled — Disables the Rapid Mode function.
• After sample rinse — Sets the wait ready signal after the sample rinse.
• After bubble elimination — Sets the wait ready signal after bubble elimination.
• After sample asp. in home — Sets the wait ready signal after sample asp. in home.
Syringe Type
Select the syringe to use in the method choosing among the options listed in the combo boxes.
• Syringe Volume (μL) — This parameter specifies the syringe volume in microliters.
• Needle Length (mm) — This parameter specifies the length of the syringe needle.
Sampling
This field comprises the following parameters.
• Sample Volume (μL) — Specify the sample volume to be pulled up into the syringe and
subsequently injected into the GC inlet. This volume is a function of the syringe
installed.
• Plunger Strokes — Specify the number of plunger strokes to eliminate air bubbles
forming during sample drawing. The range is 0 - 15.
• Air and Filling Mode — This parameter is enabled when Basic or Enrichment injection
mode has been selected. Set the parameter choosing one of the following options:
– Auto: Select this option when the automatic control of the volumes of air and sample
to be pulled up into the syringe is desired.
– Custom: Select this option when it is desired the manual control of the volumes of
air and sample to be pulled up into the syringe. This enables Air volume (μL) and
Filling volume (μL) boxes.

• Air Volume (μL) — This parameter is enabled if the Custom option has been selected in
Air and filling volumes combo box. Set the desired volume of air to be pulled up after the
needle is moved out of the vial.
• Filling Volume (μL) — This parameter is enabled if the Custom option has been
selected in Air and filling volumes combo box. Set the desired volume of sample to be
used for the syringe cleaning.
Injection
• Injection Depth — Defines the penetration depth of the syringe needle into the injector.
Set the parameter choosing one of the following options:
– Standard (default value): The syringe needle goes into the injector to the maximum
possible depth according to the injector used and to the needle length. When this
option is selected, Pre and Post-Injection Dwell Time(s) boxes are enabled.
– Minimum: The syringe needle enters the injector and stops immediately beyond the
septum. Use Minimum only if injecting with cold needle (Cold Needle technique).
When this option is selected, Pre and Post-Injection Dwell Time(s) boxes are not
enabled.
– Custom: Select Custom to be able to set the penetration depth and the injection
speed. The Fast Injection, Injection depth (mm), Penetration Depth (mm), and
Injection Speed (μl/s) boxes are enabled.
• Pre-Injection Dwell Time (s) — Specifies how long the syringe needle must remain
inside the injector before injection. The needle is heated before the sample injection (Hot
Needle Injection). It is active when Standard has been selected in Injection Depth combo
box.
• Post Injection Dwell Time (s) — Specifies how long the syringe needle must remain
inside the injector after injection. This allows the safe sample removal from the needle
before it is withdrawn. It is active when Standard has been selected in Injection Depth
combo box.
• Fast Injection — Enabled when Custom has been selected in Injection Depth combo
box. When this check box is selected, only the Injection Depth (mm) box remains active.

Fast Injection is a liquid injection technique to minimize sample discrimination generated

by the syringe needle in the Cold Needle Technique. The total dwell time of the needle into
the injector during the injection process is about 100 ms.
Fast Injections are only for use with 10 μL, 57 mm needle length syringe types.
• Injection Depth (mm) — Enabled when Custom has been selected in Injection Depth
combo box. Select the desired penetration depth of the syringe needle into the injector.
• Penetration speed (mm/s) — Specify the penetration speed of the syringe needle into
the injector.
• Injection Speed (μL/s) — This parameter specifies the transfer speed of the sample from
the syringe to the injector. This parameter is enabled when Custom has been selected in
Injection Depth combo box.
Sample Depth in Vial

• Bottom sense — Check this button to enable vial bottom sensing mode, where the
needle will go down to the bottom of the sample vial. The value height from bottom
defines the final distance between needle tip and vial bottom.
• Height from bottom (mm) — This parameter specifies the hight, from the bottom of
the vial, at which the syringe needle stops the penetration into the vial. It is enabled when
Bottom sense check box is selected.
• Sample vial depth (mm) — Select the desired penetration depth of the syringe needle
into the vial. It is enabled when Bottom sense check box is not selected.
Sample Viscosity
This field defines the sample drawing speed as a function of the sample viscosity.

GC Liquids Method Setup - 2nd Inj.Port Page
• Sample Type — This parameters defines the viscosity of the sample. Choose one of the
following options.
– Non Viscous: Select this option when the sample has low viscosity.
– Viscous: Select this option when the sample has high viscosity.
– Custom: Select this option to set the desired drawing parameters.
The boxes Sample Pull-up Speed (μL/s), Delay After Plug Strokes and Viscosity
Delay will be enabled.
• Sample Pull-up speed (μL/s) — Enabled when Custom has been selected in Sample
Type combo box. It specifies the desired sample drawing speed.
• Delay after plunger strokes (s) — Enabled when Custom has been selected in Sample
Type combo box. It specifies how long the plunger will remain in the low position after
the last stroke to eliminate bubbles before the sample drawing.
• Viscosity delay — Enabled when Custom has been selected in Sample Type combo box.
It specifies how long the plunger will remain at the top of the stroke after the sample
drawing (to account for viscous samples). This allows the sample to fill in the syringe
barrel.

This page is visualized when Double type option has been selected in the GC Liquids Method
Setup - General Page to perform the sampling in two injectors. Set here the required
parameters for the second injector.
Figure 19. GC Liquids Method Setup - Second Injection Port Page

This page comprises the following fields
Injection Port
This field defines the second injector and its injection mode.

• Injector — Select the second injector.
• Injection Mode — Choose one of the following modalities of injection:
– Confirmation: When selected, the sampler injects the same sample into two
injectors.
– Double pro: When selected, the sampler injects a different sample into each injector.
The Sample delta box is enabled.
• Sample delta — This box is enabled only when Double pro injection is selected. Specify
the interval between two batches of vials containing different samples.
• Delay Inj. 1 to Inj. 2 (s) — Indicates the waiting time before the sample is injected into
the second injector.
• Use same Sampling and Injector parameters Parameters as the first injection port —
Check this box if the second injector will use the same sampling and injection parameters
of the first one. Otherwise the programming section for the second injector parameters
will be visualized.
Set the sampling and injection parameters for the second injector proceeding as described in
GC Liquids Method Setup - General Page.

GC Liquids Method Setup - Washes Page

In this page set the parameters of pre-washing and post-washing with solvent, and washing
with sample.
Figure 20. GC Liquids Method Setup - Washes Page
This page Includes the following fields:

• Washes
• Pre-Injection
• Rinse
• Post-Injection
Washes
The autosampler can use up to four different solvents for the syringe cleaning.
The washes options are:

• Number of solvent (s) — Select the number of the cleaning solvents choosing between
the following options:
– Single: Select this option when a single cleaning solvent should be used.
– Multiple: Select this option when more than one cleaning solvents should be used.
• Wash Station — Select the washing station to use.

Pre-Injection
In this field the parameters for pre-washing with solvent are specified.
• Solvent — Specify the solvent(s) to be used according to the Single or Multiple option
selected.
When a solvent vial is used as Internal Standard or as Solvent Flush it cannot be selected
as solvent wash.
• Cycles — Set how many syringe pre-washing cycles with solvent have to be run before
injection. Set a number between 0 and 15.
• Solvent volume (μL) — Specify the volume of the pre-rinse solvent. This depends on the
syringe volume.
Rinse
In this field, the parameters of the syringe washing with sample are set.
• Rinses — Set the number of syringe pre-washing cycles with sample to be run.
• Rinse volume (μL) — Specify the volume of pre-rinse sample. This depends on the
syringe volume.
Post-Injection
In this field, the parameters of post-washing with solvent are specified.
• Solvent — Specify the solvent(s) to be used according to the Single or Multiple option
selected.
When a solvent vial is used as Internal Standard or as Solvent Flush it cannot be selected
as solvent wash.
• Cycles — Set how many syringe post-washing cycles with solvent have to be run after
injection. Set a number between 0 and 15.
• Solvent volume (μL) — Specify the volume of the post-rinse solvent. The solvent
volume depends on the syringe volume.

GC Liquids Method Setup - Synch Page
GC Liquids Method Setup - Synch Page

In this page set in which step of the sampling phase the autosampler sends the Start signal to
the GC.
Figure 21. GC Liquids Method Setup - Synchronization Page
This page comprises the following field:
GC Synchro Start
In this field select in which step of the sampling phase the autosampler will send the Start
Signal to the GC. You may choose among four different synchronism options listed below.
• Standard — Start signal is sent at the beginning of the sample injection.
• Anticipated — Start signal is generated when the sliding plate touches the injector before
injection. The GC run must start before that the injection takes place.
• Delay — Start signal is generated when the sample injection is completed. It is
particularly useful in the Large Volume Injection Technique to avoid adjustment of the time
of the parameters related to the quantity of injected solvent or to the speed of injection.
• None — No signal is sent to the GC.

GC Liquids Method Setup - Synch / Int. Std Setup Page

This page is visualized when the injection mode Internal standard Post or Standard Internal
double has been selected in the GC Liquids Method Setup - General Page.
Figure 22. GC Liquids Method Setup - Synchronization and Internal Standard Page
The autosampler supports the Internal Standard injection technique. Quantitative or

qualitative analysis may be more accurate with the automatic addition of an internal standard.
The autosampler will automatically compensate for any factors that may affect the sample
sequence. This ensures precision and accuracy of the analytical results.
The Internal Standard technique consists of programmable volumes of an internal standard
and a sample. Both are sequentially drawn from the syringe and injected together. Air gaps
can be used to separate the sample from the internal standard.
• When Internal Standard Post mode is used, a single volume of air is drawn between the
internal standard or the solvent, and the sample.
• When Internal Standard Double mode is used, a volume of air is drawn twice. The
syringe body will contain a sequence consisting of: air, internal standard, air, and sample.
This page includes the following fields:

• GC Synchro Start
• Internal Standard
• Internal Standard Depth in Vial

GC Synchro Start
Signal to the GC. You may choose among four different synchronism options listed below.
injection. The GC run will start before that the injection takes place.
Internal Standard
In this field set the Internal standard parameters.
• Int. Std volume (μL) — Indicates the volume of the internal standard.
• Air gap volume - Air 2 (μL) — Indicates the air gap between internal standard and the
sample.
• Internal standard position — Select the position of the vial containing the internal
standard.
– Tray: Select this option if the vial containing the internal standard is located in the
sampler tray. It indicates the position number.
– Standard wash station: Select this option box if the vial containing the internal
standard is located in the standard wash station. It indicates the position A, B, C or
D.
– Large wash station: Select this option box if the vial containing the internal standard
is located in the large wash station. It indicates the position A, or B.
Internal Standard Depth in Vial

• Bottom sense — Checking this button the needle goes down to the bottom of the
internal standard vial.
• Height from bottom (mm) — This parameter specifies the high, from the bottom of the
internal standard vial, at which the syringe needle stops the penetration into the vial. It is
enabled when Bottom sense check box is selected.
• Sampling vial depth (mm)— Select the desired penetration depth of the syringe needle
into the vial. It is enabled when Bottom sense check box is not selected.

GC Liquids Method Setup - Synch / Solvent Flush Page

This page is visualized when the injection mode Needle Solvent Wash, Solvent Flush Post,
Solvent Flush Double or Enrichment Needle Solvent Wash has been selected in GC
Liquids Method Setup - General Page.
Figure 23. GC Liquids Method Setup - Synchronization and Solvent Flush Page
The autosampler supports the Solvent flush injection technique which uses a plug of solvent,
which is drawn before the sample. During the injection, the solvent flushes out the sample
from the syringe needle. This technique can reduce analyte discrimination during injection.
Air gaps can be used to separate the sample from the solvent.
• When Solvent Flush Post mode is used, a single volume of air is drawn between the
solvent and the sample.
• When Solvent Flush Double mode is used, a volume of air is drawn twice. The syringe
body will contain a sequence consisting of: air, solvent, air, and sample.

• GC Synchro Start
• Solvent Flush
GC Synchro Start
signal to the GC. You may choose among four different synchronization options listed below.


injection. The GC run must start before that the injection takes place.
Solvent Flush
In this field set the solvent flush parameters.
• Solvent volume (μL) — Indicates the volume of the solvent.
• Air gap volume - Air 2 (μL) — Indicates the gap between solvent and sample
• Solvent position — Select the position of the vial containing the solvent.
– Tray: Select this option if the vial containing the solvent is located in the sampler
tray. It indicates the position number.
– Standard wash station: Select this option box if the vial containing the solvent is
located in the standard wash station. It indicates the position A, B, C or D.
– Large wash station: Select this option box if the vial containing the solvent is located
in the large wash station. It indicates the position A, or B.
• Solvent vial — Select the position of the vial containing the solvent.
• Solvent vial depth% — This parameter is visualized when the injection mode Needle
solvent wash, or Enrichment needle solvent wash has been selected.

GC Liquids Method Setup - Enrichment Page
GC Liquids Method Setup - Enrichment Page

This page is visualized when the injection mode Enrichment has been selected in the GC
Liquids Method Setup - General Page. This method can be useful when the PTV injector is
used with properly packing of the liner for the injection of gases or liquids. This method can
be instead of the normal programmed injection speed for large volume of liquids.
Figure 24. GC Liquids Method Setup - Enrichment Page

• Enrichment Parameters
Enrichment Parameters
In this field set the sample enrichment parameters.
• Enrichment injection repetitions — This parameter establishes how many repeated
injections will be performed before the start signal is sent to the GC.
• Time between enrich. inj(s) — This parameter indicates the waiting time between an
enrichment injection and the next.

GC Liquids Method Setup - Advanced Page
GC Liquids Method Setup - Advanced Page

Use the parameters in this page to refine the method.
Figure 25. GC Liquids Method Setup - Advanced Page
This page includes the following field.
Advanced Parameters
In this field set the following advanced parameters.
• Wash solvent depth (mm)— Specify at which depth of the solvent vial the syringe needle
must penetrate.
• Waste depth (mm)— Specify the depth of the waste vial the syringe needle must
penetrate.
• Needle speed in vial (mm/s) — Specify the penetration speed of the syringe needle into
the sample vial. This is set according to the septa and needle characteristics.
• Solvent filling speed (μL/s) — Specify the speed the syringe plunger is pulled up to draw
the wash solvent into the syringe. This is related to the viscosity of the solvent and the
syringe type used.
• Bubble elimin. pullup (μL/s) — Specify the speed the syringe plunger is pulled up and
pushed down as the needle is held in the sample vial. This eliminates air from the syringe,
thereby clearing it of bubbles.
• Delay between strokes (s) — Specify the delay between each plunger stroke.

GC Liquids Method Setup - Cooled/Heated Tray Holder Page
GC Liquids Method Setup - Cooled/Heated Tray Holder Page

This page is visualized when a thermostatted tray holder are installed and configured.
Figure 26. GC Liquids Method Setup - Cooled/Heated Tray Holder Page
This page includes the following field.

• Primary/Secondary Tray Temperature
Primary/Secondary Tray Temperature

These frames allow to set the following parameters:
• Run Temperature (°C) — Check this box to enable the tray holder temperature control.
The temperature is thermoregulated from 4 to 70 °C.
• St-By Temperature (°C) — Set a temperature for the tray holder to stay while the
sampler is not running (stand-by condition). The temperature is thermoregulated from 4
to 70 °C.

6
GC Headspace Method Parameters Setup

This chapter covers information for your TriPlus RSH autosampler working in GC
Headspace method mode.
The GC Headspace Method Parameters Setup pages are available if you have selected GC-
Headspace as method mode in the Instrument setup. Refer to Configuring GC Headspace
Method Mode.
GC Headspace Method Setup Page is composed of several subsidiary pages (tabs) some of
which will only be visualized following the selected options. According to the type of injector,
the syringe installed and the desired injection mode, the tab will show the suggested values
that can be changed by the operator.
GC Headspace Method Parameters Setup comprises the following tabs:

• GC Headspace Method - General Page
• GC Headspace Method - Syringe Page
• GC Headspace Method - Syringe Solvent Washes Page
• GC Headspace Method - Synchronization/Stand-by Page
• GC Headspace Method - Advanced Page

6 GC Headspace Method Parameters Setup
GC Headspace Method - General Page

In this page you will set the sampling and incubation parameters.
Figure 27. GC Headspace Method - General Page

• Injection Port
• Mode
• Syringe Type
• Analysis Time
• Incubation
• Sample
• Sampling Depth in Vial
• DoublePro 2nd Injector
Injection Port
This field comprises the following options:
• Injector — Select the injector to use in the method choosing among the options listed in
the combo box.

Mode
In this field you specify how the sample must be conditioned.
Choose among these four incubation modes:

• Constant — In this mode, the sample is sequentially conditioned at a programmed
temperature with a constant conditioning time.
• Progressive — In this mode, the sample is conditioned at a programmed temperature
with a conditioning time that increases for each sample according to a programmed
additional time. This mode is useful for developing and refining headspace methods.
• Multiple Extraction — In this mode, automatic multiple extraction steps of headspace
from the same sample vial repeatedly are performed.
• Constant DoublePro — In this mode, two samples are simultaneously conditioned at a
programmed temperature with a constant conditioning time and injected into two
separated injection ports. See DoublePro 2nd Injector.
Syringe Type
Select the syringe to use in the method choosing among the options listed in the combo boxes.
• Syringe Volume (mL) — This parameter specifies the syringe volume in milliliters.
• Needle Length (mm) — This parameter specifies the length of the syringe needle.
Analysis Time
This field contains the full runtime of a single sample. It is used for calculation of multiple
sample incubation when the time is longer than the analysis runtime. The autosampler
automatically updates sample by sample with the last runtime.
Incubation
Specify the conditioning and shaking parameters. Vial shaking is used to decrease the time
necessary for sample equilibrium.
• Agitator Temperature (°C) — The temperature is thermo-regulated from 40 to 150 °C.
• Incubation Time — This parameter specifies the incubation time for all samples to be
analyzed with the method in used. The time here specified is in minutes.
• Progressive Incubation Time (min) — This parameter becomes available when the
incubation mode is Progressive. This parameter specifies the time in minutes
progressively added to the incubation time for all samples and analyzed with the
headspace method.

• Multiple Extraction Venting Time (s) — This parameter becomes available when the
incubation mode is Multiple extraction. This parameter specifies the venting/flushing
time in seconds of the headspace gas contained in the vial, to be performed after each
injection.
• Agitator On (s) — This parameter specifies the time in seconds for shaker on. Vial
shaking is used to decrease the time necessary for sample equilibrium.
• Agitator Off (s) — This box specifies the time for shaker off.
Sample
Set the parameters for the sample drawing.
• Sample Draw (mL) — This parameter specifies the volume of vapor to be drawn and
injected. The maximum injectable value is 10% less than the syringe capacity.
• Enrichment Counts (#) — This parameter becomes available when the incubation
Mode is Constant or Progressive. This parameter specifies the number of samplings to
be carried out from the same sample vial. When Enrichment Counts is greater than 1, the
specified number of headspace samplings will be injected into the specified injector.. The
start signal to the GC is sent after the last injection.
• Enrichment Delay (min) — This parameter becomes available when the incubation
mode is Constant or Progressive. This parameter specifies the delay time in minutes
between one enrichment and the next.
Sampling Depth in Vial

Set the penetration depth of the syringe needle into the vial.
• Standard — Select this option to set the penetration depth of the needle into the vial, in
millimeters, to a pre-set depth of 25 mm.
• Custom — Select this option for manual control of the penetration depth of the needle
into the vial. The Sampling Vial Depth (mm) parameter is enabled.
• Sampling Vial Depth (mm) — This parameter becomes available when the Custom
option is selected. With this parameter, you can set the penetration depth of the needle
into the vial in millimeters.
DoublePro 2nd Injector

This field becomes available only when Constant DoublePro mode has been selected. See
Constant DoublePro Mode.
In this mode, the autosampler injects a different sample into each injector respecting the
relevant incubation times.

• Injector — Select the second injector defined in the Sampler Set-up dialog window.
• Sample delta — Specify the interval between two batches of vials containing the different
samples.
• Delay Injection1 to Injection2 (s) — This parameter specifies the time in seconds
before injecting the sample into the second injection port. To have the correct
repeatability of the retention time, set the time at a value slightly longer than the time
necessary for the autosampler to prepare the sample to inject.

GC Headspace Method - Syringe Page

In this page set the syringe, injection and speed parameters.
Figure 28. GC Headspace Method - Syringe Page

• Syringe
• Speed
• Injection
Syringe
This field contains the syringe parameters:
• Syringe Temperature (°C) — Check this box to enable the control of the syringe
temperature. The temperature is thermo-regulated from 40 to 150 °C. If this box is
unchecked, the temperature below 40 °C is not thermo-regulated but the syringe is ready
only if its temperature is really below 40 °C.
• Enable Pre-Filling — Select this check box to pressurize the vial before sampling the
headspace vapors. When the check box is selected, the volume used to pressurize the vial
is the value of the Sample Draw Volume parameter.
• Filling Volume (mL) — This parameter specifies the sample volume in milliliters to be
initially drawn into the syringe for purging.

• Filling Count (#) — Set the number of syringe plunger strokes from the same vial, to
have a homogeneous phase between the headspace gas in the vial and the sample in the
syringe.
• Filling Delay (s) — Set the delay time in seconds between the plunger pull-up and the
sample ejection, when the syringe is filled with the sample.
• Pre-Injection Syringe Flush (Fixed 5 seconds) — Select this check box for a syringe
flush cycle of five seconds before injection.
• Post-Injection Syringe Flush (s) — Set the time in seconds to be used for syringe flush
after injection.
Speed
This fields contains parameters for setting the speed of sample drawing into the syringe and
the speed of injection into the inlet.
• Filling Speed (mL/min) — This parameter specifies the speed, in milliliters per minute,
at which the sample is drawn from the headspace into the syringe.
• Injection Speed (mL/min) —This parameter specifies the transfer speed of the sample,
in milliliters per minute, from the syringe to the inlet.
Injection
This field contains the parameters for the penetration depth and the amount of time the
syringe needle resides in the injector before or after the sample is injected.
• Injection Depth (mm) — This parameter specifies the penetration depth, in millimeters,
of the syringe needle into the injector.
• Pre-Injection Delay (s) — This parameter specifies the syringe needle waiting time, in
seconds, in the injector before the sample is injected.
• Post-Injection Delay — This parameter specifies the syringe needle waiting time, in
seconds, in the injector after the sample is injected.

GC Headspace Method - Syringe Solvent Washes Page
GC Headspace Method - Syringe Solvent Washes Page

This page contains the parameters for washing the syringe.
Figure 29. GC Headspace Method - Syringe Solvent Washes Page
This tag includes the following frame:

• Washes
Washes
The autosampler can use up to four different solvents for the syringe cleaning according to the
type of washing station installed.
• Number of solvent (s) — Select the number of the cleaning solvents choosing between
the following options:
– Single: Select this option button when a single cleaning solvent should be used.
– Multiple: Select this option button when more than one cleaning solvent should be
used.
• Wash Station — Select the washing station to use.
• Clean — This parameter allows to set how many syringe cleaning cycles with solvent
have to be run.
• Solvent — This parameter specifies the solvent(s) to be used according to the Single or
Multiple option selected.

GC Headspace Method - Synchronization/Stand-by Page
• Dry time (min) — This parameter specifies the time required to dry the syringe after
having performed the cleaning with the solvent.
• Wash frequency — Specify how many samples after which the syringe cleaning must be
performed.

In this page you can set the autosampler stand-by condition parameters and the sampling
phase step in which the autosampler sends the start signal to the GC
.
Figure 30. GC Headspace Method - Synchronization/Stand-by Page

• Wait for Readiness Flags
• Stand-by Syringe Flush
• GC Synch Start Mode
• Anticipated Synchronization Before Incubation End
Wait for Readiness Flags

In this field set the stand-by temperatures when the autosampler is not running (stand-by
condition).

• Syringe — Check this box to set the syringe temperature in °C, when the Autosampler is
not running (stand-by condition).The control of the stand-by agitator temperature is
enabled. The temperature is thermo-regulated from 40 to 150 °C. If this box is
unchecked, the temperature below 40 °C is not thermo-regulated but the agitator is ready
only if its temperature is actually below 40 °C.
• Agitator — Check this box for setting the temperature of the agitator (incubation oven)
in °C, when the sampler is not running (stand-by condition).The control of the stand-by
syringe temperature is enabled. The temperature is thermo-regulated from 40 to 150 °C.
If this box is unchecked, the temperature below 40 °C is not thermo-regulated but the
syringe is ready only if its temperature is actually below 40 °C.
Stand-by Syringe Flush

This field contains the parameter Syringe Flush to enable/disable syringe wash with inert gas
when the autosampler is not running (stand-by condition).
GC Synch Start Mode

This field contains parameters to specify the synchronisation mode between the autosampler
and the GC
• Normal — Select this option to start the GC at the end of sample injection.
• Anticipated — Select this option to start the GC before sample injection.
Anticipated Synchronization Before Incubation End

In the anticipated synchronization mode, the autosampler generates a pre-trigger signal before
the end of the sample conditioning time to start trap cooling. This minimizes the time the
trap remains at the trapping temperature, thus saving on cooling agent.
• Anticipated Time (min) — This parameter specifies the time in minutes required to
reach the cold trap temperature.

GC Headspace Method - Advanced Page

In this page you can set advanced parameters for refinement of the GC Headspace method.
Figure 31. GC Headspace Method - Advanced Page
This page includes the following field:

• Advanced Parameters
Advanced Parameters
In this field set the following parameter:
• Needle Speed In Vial (mm/s) — Set the penetration speed, in millimeters per second, of
the syringe needle into the sample vial.


7
SPME Method Parameters Setup

This chapter covers information for your TriPlus RSH autosampler working in SPME
method mode.
The SPME Method Parameters Setup pages are available if you have selected SPME as
method mode in the Instrument setup. Refer to Configuring SPME Method Mode.
SPME Method Setup Page comprises the following tabs:

• SPME Method Setup - Parameters Page
• SPME Method Setup - Fiber Conditioning Page

7 SPME Method Parameters Setup
SPME Method Setup - Parameters Page

In this page you set the parameters related to sampling, incubation and injection for the
SPME method.
Figure 32. SPME Method - Parameters Page

• Desorb Port
• Sample Vial Parameters
• Mode
• Sampling Vial Depth
• Incubation
• Injection
• Analysis Time
• GC Synch Start Mode
Desorb Port
This field comprises the following option:
• Injector — Select the injector to use in the method, choosing among the options listed in
the combo box.

Sample Vial Parameters

This field includes the following parameters.
• Needle Speed in Vial — This parameter specifies the speed, in millimeters per second, of
the protective needle into the vial.
• Extract Time — This parameter specifies the extraction time, in minutes, of the fiber
into the vial located in the incubation oven.
• Progressive Extract Time — This parameter becomes available when the incubation
mode is set to Progressive. This parameter specifies the time progressively added to the
extraction time of the fiber into the vial located in the incubation oven.. The time here
specified is in minutes.
Mode
This field specifies how the sample must be conditioned.
• Incubation Mode — Choose between two incubation modes:
– Constant: In this mode the sample is sequentially conditioned at a programmed
temperature with a constant conditioning time.
– Progressive: In this mode the sample is conditioned at a programmed temperature
with a conditioning time that increases for each sample according to a programmed
additional time.
Sampling Vial Depth

This field contains parameters to set the penetration depth of the protective needle into the
vial.
• Sampling Depth Mode
– Standard — Select this option to set the protective needle penetration into the vial at
a predefined depth.
– Custom — Select the option for manual control of the depth of penetration of the
protective needle into the vial. The parameter Sampling Vial Depth (mm) is enabled.
– Sampling Vial Depth — This parameter becomes available when the
Custom button is clicked. This parameter specifies the penetration
depth in millimeters of the SPME needle into the vial.
The value reflects the total length including the length of the fiber.

Incubation
This field contains the conditioning and shaking parameters. Vial shaking is used to decrease
the time necessary for the sample equilibrium.
• Agitator Temperature (°C) — Set a temperature for the agitator (incubation oven) while
the autosampler is not running (stand-by condition). The temperature is
thermo-regulated from 40 to 150 °C.
• Agitator wait for readiness — Check this box to enable the control of the temperature.
The temperature is thermo-regulated from 40 to 150 °C. If this box is unchecked, the
temperature below 40 °C is not thermo-regulated but the agitator is ready only if its
temperature is actually below 40 °C.
• Incubation Time — Specify the incubation time for all samples to be analyzed with the
method in use. This time is given in minutes.
• Agitator On — Specifies the time for shaker on. The vial shaking is used to decrease the
time in seconds necessary for the sample equilibrium.
• Agitator Off — Specifies the time for shaker off.
Injection
This field includes the following injection parameters.
• Injector Depth (mm) — Specifies the depth in millimeters of the protective
needle into the injector. The value reflects the total length including the
length of the fiber.
• Penetration speed (mm/s) — Set the penetration speed of the fiber into the injector.
This speed is set according to the septa and needle characteristics.
• Desorption Time (min) — This parameter specifies the desorption time in minutes of
the fiber into the injector.
Analysis Time
This box contains the full runtime of a single sample and it is used for calculation of the
multiple sample incubation times when the incubation time is longer than the analysis
runtime. The autosampler automatically sample by sample with the last runtime.
• Analysis Time (min) — Specifies the full runtime in minutes of a single sample.

SPME Method Setup - Fiber Conditioning Page
GC Synch Start Mode

This field includes the parameters to synchronize GC Start.
• When Needle enters Injector — Select this option to send the start signal to the GC at
the beginning of the protective needle penetration into the injector.
• Delayed — Select the option to send the start signal to the GC after the fiber is exposed.

In this page you can set the fiber conditioning parameters for the SPME method.
Figure 33. SPME Method - Fiber Conditioning Page

• Derivatization
• Conditioning Port
• Fiber Conditioning Station
Derivatization
This field includes the Derivatization parameters:

• Derivatization Station — Select the derivatization station.

• Derivatization Vial — Specify the position of the derivatization vial in the derivatization
station.
• Derivatization Ads. Time (min) — This parameter becomes available when the
Derivatisation Vial parameter is set. This parameter specifies the exposure time in
minutes of the fiber in the Derivatization.
• Derivatization Depth (mm) — This parameter becomes available when the
Derivatization Vial parameter is set. Select the desired derivatization depth
of the fiber into the vial. The value reflects the total length including the
length of the fiber.
Conditioning Port
This field includes the following parameters.
• Port ID — This parameter specifies the injection port or the fiber conditioning port to
be used for fiber back out.
• Pre Desorption Time (min) — This parameter specifies the pre desorption time in
minutes of the fiber in the conditioning port.
• Post Desorption Time (min) — This parameter specifies the post desorption time in
minutes of the fiber in the conditioning port.
Fiber Conditioning Station

This field includes the following parameter.
• Conditioning Temperature (°C) — Set the fiber conditioning temperature according to
the type of fiber in use. The temperature is thermo-regulated from 40 °C to 350 °C.

8
Custom Method
Custom Method automates basic sample preparation as Calibration Dilution, Sequential
Dilution, Sample Standard Addition, and Derivatization.
Custom Methods allow you to select a custom method from a set of methods. Opening a
custom method loads all of the necessary parameters. Navigate to the folder where custom
methods are installed to locate the desired method.
Note Initially, no custom methods come pre-installed with the TriPlus.RSH. However,
custom methods may be supplied to support specific requirements. These methods may
be loaded by selecting Load Custom Method from the TriPlus RSH menu.

8 Custom Method

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Thermo Xcalibur

TriPlus RSH Autosampler Setup

P/N 31709650, Revision B, March 2013

Release history: Revision A, September 2011; Revisione B, March 2013

The Xcalibur manuals support Xcalibur version 2.2 and above.

For Research Use Only. Not for use in diagnostic procedures.

Chapter 1 TriPlus RSH Configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Chapter 2 TriPlus RSH Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5

Chapter 3 TriPlus RSH Instrument Setup Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9

Thermo Scientific manual-name iii

Start Syringe Clean Cycle Off-line. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Chapter 4 Instrument Method Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21

Chapter 5 GC Liquids Method Parameters Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27

iv manual-name Thermo Scientific

GC Liquids Method Setup - Cooled/Heated Tray Holder Page . . . . . . . . . . . . 44

Chapter 6 GC Headspace Method Parameters Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45

Chapter 7 SPME Method Parameters Setup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57

Chapter 8 Custom Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63

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For more information, including upcoming application notes, visit

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CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION

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TriPlus RSH Configuration

Thermo Scientific TriPlus RSH Autosampler Setup 1

Instrument Configuration Dialog Window

To open this view choose Start | Programs | Thermo Foundation | Instrument

Figure 1. TriPlus RSH Configuration Dialog Window

Configuration windows is subdivided into the following fields:

Configuration window includes the following common buttons:

2 TriPlus RSH Autosampler Setup Thermo Scientific

Configure the sampler as one of the following:

Thermo Scientific TriPlus RSH Autosampler Setup 3

Disable hand-held Control

Extract the Injection Volume from the Sequence

If not checked, the injection volume of the method is used.

Sequence Error Handling

4 TriPlus RSH Autosampler Setup Thermo Scientific

TriPlus RSH Status

Thermo Scientific TriPlus RSH Autosampler Setup 5

Road Map Home Page Status Tabs

This tab comprises the following options:

6 TriPlus RSH Autosampler Setup Thermo Scientific

Thermo Scientific TriPlus RSH Autosampler Setup 7

8 TriPlus RSH Autosampler Setup Thermo Scientific

TriPlus RSH Instrument Setup Menu

Thermo Scientific TriPlus RSH Autosampler Setup 9

Figure 4. TriPlus RSH Menu

The pull-down menu contains the following options:

10 TriPlus RSH Autosampler Setup Thermo Scientific