DIGESTION RATE ON FISH
Name : Anang Yanuar Ramadhan
Student ID : B1B015015
Entourage :I
Group :4
Assistant : Rosewitha Anggraeni Privieshanti H.
PRACTICAL REPORT OF ANIMAL PHYSIOLOGY II
MINISTRY OF EDUCATION AND CULTURE
JENDERAL SOEDIRMAN UNIVERSITY
FACULTY OF BIOLOGY
PURWOKERTO
2020
I. INTRODUCTION
A. Background
Digestion rate is time that needed to break food from complex molecules to
simpler molecules (Juansang et al., 2012). Digestion breaks down flour and
protein or biopolymers into monomer units using enzymes (Qiao et al., 2016).
Digestion rate is generally correlated with the rate of fish metabolism. The longer
the time, the stomach contents will be reduced so that the body weight of the fish
is reduced. Gastric emptying time is also influenced by the food consumed by fish
(Diniarti & Astriana, 2019).
The factors that influence the rate of digestion are water temperature because
fish are poikiloterm. Digestion rate or rate of emptying the stomach is not only
influenced by the temperature of the water but also by the food consumed.
Differences in feed quality will cause differences in the constituent components,
and this difference will result in differences in the rate and ability of feed
digestion. Fish food is food that is specially made or produced to be easy and
available to eat. Fish food is very influential on the growth and survival of fish
bodies (Zidni et al., 2018).
Catfish (Clarias gariepinus) are used in this laboratory activity because
catfish have a true stomach so it is easy to observe. Besides that catfish are
classified as eaters of all (omnivorous). Catfish are able to eat all types of food
and are also classified as scavengers. It is naturally nocturnal and catfish it is able
to adapt. The other characteristic or behavior that stands out is when it encounters
a new atmosphere, for example a sudden change of atmosphere from a dark to a
bright place (Fujaya, 2002).
B. Purpose
The objectives of this laboratory activity are
1. The shape of empty gastric and gastric contains feed
2. Obtain a skill of isolating the fish gastric
3. Calculate the rate of gastric emptying
II. MATERIAL AND METHODS
A. Material
The materials that used in this practice are catfish, fish feed (in the form of
pellet).
The tools that used in this practice are analytical scale, aquarium, dissection
kit, and specimen tray.
B. Methods
1. Aquarium is prepared then filled with water as high as 25 cm.
2. Fish is spread in the aquarium.
3. Pellet is inserted into aquarium as much as 2,5 % from the fish weight, and
fish is let to consume the feed for 15-20 minutes.
4. Fish weight is weighted on analytical scale.
5. Fish is dissected to take out the gastric of fish, and do the weighting also.
The obtained gastric weight is stated as 0 minute after eating (Bx).
6. Step 4 and 5 is re-perform after 30 menit (By) and 60 menit (Bz).
III. RESULT AND DISCUSSION
A. Result
Table 3.1. The Result of Digestion Rate Observation on Catfish (Clarias
gariepinus)
X (0’) Y (30’) Z (60’)
Group
Bx (gr) Bx (%) By (gr) By (%) Bz (gr) Bz (%)
1 2,06 1,61 1,87 1,43 2,07 1,51
2 0,61 0,44 1,75 1,25 1,94 1,37
3 2,26 1,5 2,22 1,55 1,23 0.98
4 1,6 1,36 2,27 1,75 2,26 1,49
5 2,08 1,58 2,11 1,56 1,97 1,56
Calculation of Group 4
Bx 1,6
Bx (%) = x 100% = x 100% = 1,36 %
Bxt 117
By 2,27
By (%) = x 100% = x 100% = 1,75 %
Byt 129
Bz 2,26
Bz (%) = x 100% = x 100% = 1,49 %
Bzt 151
1.8
1.6
1.4
1.2 Group 1
Group 2
1
Group 3
0.8 Group 4
Group 5
0.6
0.4
0.2
0
Bx% By% Bz%
Graphic 3.1 The Result of Digestion Rate Observation on Catfish (Clarias
gariepinus)
B. Discussion
Based on data obtained by group 4, Bx fish weight is obtained which is 117
gram, fish gastric weight is 1,6 gram, and fish gastric weight percentage is 1,36%. By
fish weight is 129 gram, fish gastric weight is 2,27 gram, and fish gastric weight
percentage is 1,75%. Bz fish weight is 151 gram, fish gastric weight is 2,26 gram,
and fish gastric weight percentage is 1,49%. Based on data obtained from group 1,
Bx fish gastric weight is 2,06 gram, and fish gastric weight percentage is 1,61%. By
fish gastric weight is 1,87 gram, and fish gastric weight percentage is 1,43%. Bz fish
gastric weight is 2,07 gram, and fish gastric weight percentage is 1,51%. Based on
data obtained from group 2, Bx fish gastric weight is 0,61 gram, and fish gastric
weight percentage is 0,44%. By fish gastric weight is 1,75 gram, and fish gastric
weight percentage is 1,25%. Bz fish gastric weight is 1,94 gram, and fish gastric
weight percentage is 1,37%.
Based on data obtained from group 3, Bx fish gastric weight is 2,26 gram,
and fish gastric weight percentage is 1,5%. By fish gastric weight is 2,22 gram, and
fish gastric weight percentage is 1,55%. Bz fish gastric weight is 1,23 gram, and fish
gastric weight percentage is 0,98%. Based on data obtained from group 5, Bx fish
gastric weight is 2,08 gram, and fish gastric weight percentage is 1,58%. By fish
gastric weight is 2,11 gram, and fish gastric weight percentage is 1,56%. Bz fish
gastric weight is 1,97 gram, and fish gastric weight percentage is 1,56%. An increase
and decrease in the rate of digestion is happened. This is likely to occur because each
group is given different catfish. The rate of digestion is also influenced by the food
consumed, because in the food consumed by fish there are many mineral contents
that will be absorbed by the intestines of fish through the digestive process that takes
place while the fish consume the food (Kimball, 1983).
Based on data obtained from group 4, Bx gram; Bx percentage; By gram; By
percentage; Bz gram and Bz percentage is different from another group because the
differences in gastric weight and fish weight. Digestion rate is generally correlated
with the metabolism rate on fish. Measuring the time during the laboratory activity
for 15 minutes and 30 minutes, so the results obtained during the laboratory activity
most likely do not constitute a digestion process due to the feed given during the
laboratory activity. Usually the more fish activity, it will require a lot of energy so
that the process of metabolism is high and requires food of a much better quality and
more in quantity. Some factors that influence the gastric weight include the size of
the organism that is vary because the less the organism, the less the organism eats
feed. Digestion rate reduction can also be caused by fish not constantly eating the
feed provided (Srichanun, 2012).
IV. CONCLUSION
Based on the result can be concluded that :
1. The fish, fish gastric weight, and fish gastric percentage on each group is
different.
2. Digestion rate on fish is correlated with metabolism rate on fish.
3. The digestion rate on Bx is 1,36%, on By is 1,75% and on Bz is 1,49%.
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